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The map label for this gene is pyk [H]

Identifier: 209550865

GI number: 209550865

Start: 3376521

End: 3377960

Strand: Reverse

Name: pyk [H]

Synonym: Rleg2_3289

Alternate gene names: 209550865

Gene position: 3377960-3376521 (Counterclockwise)

Preceding gene: 209550866

Following gene: 209550864

Centisome position: 74.44

GC content: 64.65

Gene sequence:

>1440_bases
ATGAAGCGTAATCGCAAAATTAAAATCCTCGCCACCCTCGGGCCCGCCTCCGCTGAGGAATCGATGATCGAGAAGCTGCA
TCAGGCCGGTGCCGATGTCTTCCGCATCAATATGAGCCATGCGAGCCACGACGTGATGCGTACGCTCATCCAGCGCATCC
GCTCGGTCGAGGCGCGCTCCGGCCGGCCGATCGGCATTCTTGCCGACCTGCAGGGACCGAAACTGCGAGTCGGCAAGTTC
GTCGACAGCAAGGTCGACCTGAAACCCGGCCAGACCTTCACGCTCGACAACAACGAAGCGCTCGGCGATCAGAACCGCGT
CTATCTGCCGCACCCCGAGATCCTGGAATCGGTGCAGCCCGGCCACCGCCTGCTGATCGACGACGGCAAGCTCGCGCTGC
GCGCCGAAAAATGCGACGGCAAGAGCATCGTCACGACGGTTATTTCGGGCACGCGCATTTCCGACCGCAAGGGCGTCAGC
CTTCCCGACACGCTGCTCGGCGTCGGCGCGCTGACGGACAAGGACCGCGCCGATCTCGACGCTGTGCTTGCCACCGACGA
TGTCGACTGGGTGGCGCTGTCCTTCGTCCAGCGTCCTGATGACCTTGCCGAAGTGCGCAAGATCGCCCGTGGCCGTGTCG
GCCTGATGTCGAAGATCGAAAAACCGCAGGCTCTCGAGCGTATCGAGGAAATCATCGAGCTTTCCGACGCCTTGATGGTC
GCTCGTGGCGATCTCGGCGTCGAAATGCCGCTCGAATCGGTTCCCGGCATCCAGAAGCAGCTGATCCGCGCTTGCCGCCG
TTCGGGCAAGCCGGTGGTCGTCGCCACGCAGATGCTGGAATCGATGATCTCTGCGCCGGTGCCGACGCGCGCGGAAGTTT
CCGACGTCGCAACCGCCGTCTTCGAAGGCGCCGATGCCGTCATGCTCTCGGCCGAGTCGGCCTCGGGCGACTATCCGGTC
GAAGCCGTTTCGACCATGGCGTCGATTGCCACCGCCATCGAGCGCGAGCCGCATTATCCGGGCATCATCTATGCCCAGCG
CGCCCAGCCGGAAGCGACCGGTGCCGATGCGATTTCGCTCGCTGCGCGCCAGATCGCCGAGACGCTGAAGCTGTCGGCGA
TCGTCTGTTACACCTCGTCCGGCACGACAGGCCTTCGCGCCTCGCGTGAGCGTCCGCAGGTGCCGATCCTGGCGCTGTCG
CCGATCATCAAGACGGCACGCCGCCTTGCCGTCGTCTGGGGTCTGCATTGCGTCGTCACGCATGACGCGACCGATCTCGA
CGACATGGTCAACCGCGCCTGCCGCATCGTCGCCGACGAAGGCTTCGGCAAGCCCGGCGACCGCATCATCATCTCGGCCG
GCGTGCCGCTCGGCACACCCGGCGCCACCAATATGATCCGCATCGCCTATATCGGCTCCGACGGCCAGAGCGGCATCTGA

Upstream 100 bases:

>100_bases
GTTACCAGAAGATGGGTTTCAAGGAATATGACACGGGCCGCCAGGGCAGCTGGATGGTCCAACTCTCCGACACCCCAGGG
ACGCAAGAAAGCCAGAATTG

Downstream 100 bases:

>100_bases
TCCGATCGCATCCCTTCGAAACCCCGCCGCCTCAACGAAGCGGCGGGGTTTTTCGTTGATGGCCCGGGCATTGCCAAGGG
CGGCGGTGCATGCAAGTTTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKRNRKIKILATLGPASAEESMIEKLHQAGADVFRINMSHASHDVMRTLIQRIRSVEARSGRPIGILADLQGPKLRVGKF
VDSKVDLKPGQTFTLDNNEALGDQNRVYLPHPEILESVQPGHRLLIDDGKLALRAEKCDGKSIVTTVISGTRISDRKGVS
LPDTLLGVGALTDKDRADLDAVLATDDVDWVALSFVQRPDDLAEVRKIARGRVGLMSKIEKPQALERIEEIIELSDALMV
ARGDLGVEMPLESVPGIQKQLIRACRRSGKPVVVATQMLESMISAPVPTRAEVSDVATAVFEGADAVMLSAESASGDYPV
EAVSTMASIATAIEREPHYPGIIYAQRAQPEATGADAISLAARQIAETLKLSAIVCYTSSGTTGLRASRERPQVPILALS
PIIKTARRLAVVWGLHCVVTHDATDLDDMVNRACRIVADEGFGKPGDRIIISAGVPLGTPGATNMIRIAYIGSDGQSGI

Sequences:

>Translated_479_residues
MKRNRKIKILATLGPASAEESMIEKLHQAGADVFRINMSHASHDVMRTLIQRIRSVEARSGRPIGILADLQGPKLRVGKF
VDSKVDLKPGQTFTLDNNEALGDQNRVYLPHPEILESVQPGHRLLIDDGKLALRAEKCDGKSIVTTVISGTRISDRKGVS
LPDTLLGVGALTDKDRADLDAVLATDDVDWVALSFVQRPDDLAEVRKIARGRVGLMSKIEKPQALERIEEIIELSDALMV
ARGDLGVEMPLESVPGIQKQLIRACRRSGKPVVVATQMLESMISAPVPTRAEVSDVATAVFEGADAVMLSAESASGDYPV
EAVSTMASIATAIEREPHYPGIIYAQRAQPEATGADAISLAARQIAETLKLSAIVCYTSSGTTGLRASRERPQVPILALS
PIIKTARRLAVVWGLHCVVTHDATDLDDMVNRACRIVADEGFGKPGDRIIISAGVPLGTPGATNMIRIAYIGSDGQSGI
>Mature_479_residues
MKRNRKIKILATLGPASAEESMIEKLHQAGADVFRINMSHASHDVMRTLIQRIRSVEARSGRPIGILADLQGPKLRVGKF
VDSKVDLKPGQTFTLDNNEALGDQNRVYLPHPEILESVQPGHRLLIDDGKLALRAEKCDGKSIVTTVISGTRISDRKGVS
LPDTLLGVGALTDKDRADLDAVLATDDVDWVALSFVQRPDDLAEVRKIARGRVGLMSKIEKPQALERIEEIIELSDALMV
ARGDLGVEMPLESVPGIQKQLIRACRRSGKPVVVATQMLESMISAPVPTRAEVSDVATAVFEGADAVMLSAESASGDYPV
EAVSTMASIATAIEREPHYPGIIYAQRAQPEATGADAISLAARQIAETLKLSAIVCYTSSGTTGLRASRERPQVPILALS
PIIKTARRLAVVWGLHCVVTHDATDLDDMVNRACRIVADEGFGKPGDRIIISAGVPLGTPGATNMIRIAYIGSDGQSGI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=36.3449691991786, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=36.3449691991786, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=36.5503080082135, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=36.2903225806452, Blast_Score=258, Evalue=6e-69,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=36.2903225806452, Blast_Score=258, Evalue=7e-69,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=35.4166666666667, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=35.4166666666667, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=31.0924369747899, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=31.0924369747899, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI310128738, Length=213, Percent_Identity=30.9859154929577, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=38.8655462184874, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI1787965, Length=464, Percent_Identity=35.7758620689655, Blast_Score=273, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=35.0515463917526, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI71984406, Length=483, Percent_Identity=35.1966873706004, Blast_Score=248, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI71984413, Length=483, Percent_Identity=35.1966873706004, Blast_Score=248, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI17506829, Length=483, Percent_Identity=35.1966873706004, Blast_Score=248, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI17506831, Length=483, Percent_Identity=35.1966873706004, Blast_Score=247, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=33.2644628099174, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=33.0612244897959, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI28571814, Length=485, Percent_Identity=36.0824742268041, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24648964, Length=485, Percent_Identity=36.0824742268041, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24648966, Length=387, Percent_Identity=36.6925064599483, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=30.6584362139918, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24646914, Length=241, Percent_Identity=39.4190871369295, Blast_Score=159, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51400; Mature: 51400

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNRKIKILATLGPASAEESMIEKLHQAGADVFRINMSHASHDVMRTLIQRIRSVEARS
CCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCC
GRPIGILADLQGPKLRVGKFVDSKVDLKPGQTFTLDNNEALGDQNRVYLPHPEILESVQP
CCCEEEEECCCCCCEEECHHCCCCCCCCCCCEEEECCCCCCCCCCCEECCCHHHHHHCCC
GHRLLIDDGKLALRAEKCDGKSIVTTVISGTRISDRKGVSLPDTLLGVGALTDKDRADLD
CCEEEEECCCEEEEEECCCCCEEEHHHCCCCEECCCCCCCCCHHHHHCCCCCCCCCCCCE
AVLATDDVDWVALSFVQRPDDLAEVRKIARGRVGLMSKIEKPQALERIEEIIELSDALMV
EEEECCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEE
ARGDLGVEMPLESVPGIQKQLIRACRRSGKPVVVATQMLESMISAPVPTRAEVSDVATAV
EECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHH
FEGADAVMLSAESASGDYPVEAVSTMASIATAIEREPHYPGIIYAQRAQPEATGADAISL
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHH
AARQIAETLKLSAIVCYTSSGTTGLRASRERPQVPILALSPIIKTARRLAVVWGLHCVVT
HHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCEEEEEE
HDATDLDDMVNRACRIVADEGFGKPGDRIIISAGVPLGTPGATNMIRIAYIGSDGQSGI
CCCCCHHHHHHHHHEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MKRNRKIKILATLGPASAEESMIEKLHQAGADVFRINMSHASHDVMRTLIQRIRSVEARS
CCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCC
GRPIGILADLQGPKLRVGKFVDSKVDLKPGQTFTLDNNEALGDQNRVYLPHPEILESVQP
CCCEEEEECCCCCCEEECHHCCCCCCCCCCCEEEECCCCCCCCCCCEECCCHHHHHHCCC
GHRLLIDDGKLALRAEKCDGKSIVTTVISGTRISDRKGVSLPDTLLGVGALTDKDRADLD
CCEEEEECCCEEEEEECCCCCEEEHHHCCCCEECCCCCCCCCHHHHHCCCCCCCCCCCCE
AVLATDDVDWVALSFVQRPDDLAEVRKIARGRVGLMSKIEKPQALERIEEIIELSDALMV
EEEECCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEE
ARGDLGVEMPLESVPGIQKQLIRACRRSGKPVVVATQMLESMISAPVPTRAEVSDVATAV
EECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHH
FEGADAVMLSAESASGDYPVEAVSTMASIATAIEREPHYPGIIYAQRAQPEATGADAISL
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHH
AARQIAETLKLSAIVCYTSSGTTGLRASRERPQVPILALSPIIKTARRLAVVWGLHCVVT
HHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCEEEEEE
HDATDLDDMVNRACRIVADEGFGKPGDRIIISAGVPLGTPGATNMIRIAYIGSDGQSGI
CCCCCHHHHHHHHHEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA