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The map label for this gene is rhtB [H]

Identifier: 209550864

GI number: 209550864

Start: 3375754

End: 3376389

Strand: Reverse

Name: rhtB [H]

Synonym: Rleg2_3288

Alternate gene names: 209550864

Gene position: 3376389-3375754 (Counterclockwise)

Preceding gene: 209550865

Following gene: 209550860

Centisome position: 74.4

GC content: 63.52

Gene sequence:

>636_bases
ATGGATATCGTTACGCTCATGGCTTTTGCAGCCGTTTCCTTTGTCGGCATCGCCACGCCGGGGCCGACAGTGCTGCTGGC
GCTGACCAACGGTTCGCGGCATGGCCTGCGCCGGGCAATGGCCGGCATGATCGGTGCGGTCCTCTCCGATTTCGTGCTGA
TCGGTGCCGTGGCGGTCGGGCTCGGCGCGTTGCTGGCGGCATCGGAATTCTGGTTCTCGATGCTGAAATGGGCAGGTGTT
GCCTATCTCGCCTTTCTCGGCATCATGCTGCTGCGCTCGAAGGGCGGGATCGATGCGGCGTTGAAAGCAGGTGCGCCGGC
GGGGGCGACGTCGACTTTCTCGATCGGACTGAAGAGCTTCATGGTCGCGGCGACCAATCCGAAGGGTTATTTGTTCTTCT
CGGCCTTCCTGCCGCAATTCATCGATCCGGCGCTGCCGCAGGCAACGCAATATGCGCTGCTCGCGCTCACCTTTGCCGTG
CTCGATTTCCTCATCATGTTCGGCTACGCCTTCTTCGGCTCGCAGGCGGTGCGTTTCCTGAAAACCTCGGGCGCCCTATG
GCTGGAGCGGGCCTGCGGCGGCGCGCTGCTGGCGCTTGCCGGCTCGCTGGCCTTCTATCGCCGGGCCACCGCTTAA

Upstream 100 bases:

>100_bases
TCAACGAAGCGGCGGGGTTTTTCGTTGATGGCCCGGGCATTGCCAAGGGCGGCGGTGCATGCAAGTTTCCTTCCCTATAG
CCAAGTTGGGAGGGATCTTC

Downstream 100 bases:

>100_bases
GGTCAGGCTTAAGGCAGGGATAGGTGACCGACGCGATAGGCCTGCATCTGCTCAGCACTCTCGGGGATCTCTCGCCATAG
CCTGAAGCGCACCCGTGTCC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MDIVTLMAFAAVSFVGIATPGPTVLLALTNGSRHGLRRAMAGMIGAVLSDFVLIGAVAVGLGALLAASEFWFSMLKWAGV
AYLAFLGIMLLRSKGGIDAALKAGAPAGATSTFSIGLKSFMVAATNPKGYLFFSAFLPQFIDPALPQATQYALLALTFAV
LDFLIMFGYAFFGSQAVRFLKTSGALWLERACGGALLALAGSLAFYRRATA

Sequences:

>Translated_211_residues
MDIVTLMAFAAVSFVGIATPGPTVLLALTNGSRHGLRRAMAGMIGAVLSDFVLIGAVAVGLGALLAASEFWFSMLKWAGV
AYLAFLGIMLLRSKGGIDAALKAGAPAGATSTFSIGLKSFMVAATNPKGYLFFSAFLPQFIDPALPQATQYALLALTFAV
LDFLIMFGYAFFGSQAVRFLKTSGALWLERACGGALLALAGSLAFYRRATA
>Mature_211_residues
MDIVTLMAFAAVSFVGIATPGPTVLLALTNGSRHGLRRAMAGMIGAVLSDFVLIGAVAVGLGALLAASEFWFSMLKWAGV
AYLAFLGIMLLRSKGGIDAALKAGAPAGATSTFSIGLKSFMVAATNPKGYLFFSAFLPQFIDPALPQATQYALLALTFAV
LDFLIMFGYAFFGSQAVRFLKTSGALWLERACGGALLALAGSLAFYRRATA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=185, Percent_Identity=36.2162162162162, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788099, Length=169, Percent_Identity=28.4023668639053, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21980; Mature: 21980

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIVTLMAFAAVSFVGIATPGPTVLLALTNGSRHGLRRAMAGMIGAVLSDFVLIGAVAVG
CHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGALLAASEFWFSMLKWAGVAYLAFLGIMLLRSKGGIDAALKAGAPAGATSTFSIGLKSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHH
MVAATNPKGYLFFSAFLPQFIDPALPQATQYALLALTFAVLDFLIMFGYAFFGSQAVRFL
HEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTSGALWLERACGGALLALAGSLAFYRRATA
HHCCCHHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIVTLMAFAAVSFVGIATPGPTVLLALTNGSRHGLRRAMAGMIGAVLSDFVLIGAVAVG
CHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGALLAASEFWFSMLKWAGVAYLAFLGIMLLRSKGGIDAALKAGAPAGATSTFSIGLKSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHH
MVAATNPKGYLFFSAFLPQFIDPALPQATQYALLALTFAVLDFLIMFGYAFFGSQAVRFL
HEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTSGALWLERACGGALLALAGSLAFYRRATA
HHCCCHHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]