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The map label for this gene is dctB [H]

Identifier: 209550746

GI number: 209550746

Start: 3254779

End: 3256572

Strand: Reverse

Name: dctB [H]

Synonym: Rleg2_3170

Alternate gene names: 209550746

Gene position: 3256572-3254779 (Counterclockwise)

Preceding gene: 209550748

Following gene: 209550745

Centisome position: 71.76

GC content: 63.38

Gene sequence:

>1794_bases
GTGCCGCTGATCATCTTCGCCTTCTTCACCTATGGAAGCGCAATCGCGACACGCGCCTATATGGGGGAGGCCTCGGCGCA
GGCGGGAACGGCGCTGCGCCTTGCCGTCTCGGCGCTCAGCGGTCACCTCAACCGCTACGAGGCGCTGCCGGCGCTGATCG
CCGACCACGACGACATCAAGGAACTGGTGAGCGCGCCCGACGACACAGCCTTGCGCGACGCGGCCAATCTCTATCTCAAG
GAGATCAACGGGCTGCTGAAATCCTCCGATATTTATGTGGTCAAGCCGGATGGAGAGACGATCGCGGCCAGCAATTACGA
CGGACCGGGAAGCTTCGTCGGACAGAATTTCAGCTATCGGCCGTATTTCCAGGATGCGATCGAAGGCCGGCAGGCGCGGT
TCTATGCGCTCGGCACGACCTCGCTGAAGCGCGGCTATTATTTCGCTGCGCCGATCAGGACCGGCGCGGATATTCGCGGC
GTCATCGTCTTCAAGGTCGATATCGACATGATCGAATCCTCCTGGGGCGGTGGCGAATACAAGATCTTCGTCTCCGATCC
TGAAGGCATCATCTTCATGTCCGGCAGCCCGGAATGGCTTTACGGCGCAATCCTGCCGCTGACGGCCGACCGTATCGCAC
GCACGGAAGCATCGCGCCGTTATGCCAATGCCAGGCTGACGGAGTTGCCGGTGACGCGCCAGCGCTTTGAGCCGCATGAA
CTGATGACCCTTGTCGGCGAACGGGGCTCAAGCGAATATCTGGTGCTTTCGCACTACATGTCGGCCGAGGACTGGACGGT
GAACGTGCTGATGGAGACCAGCTCCATCCGCACCCAGGCGCGCACCGCGCTTGCGGCGGTCTTTCTCATCCTCTGCATTG
CCGGGCTCACGGTCGCGGTTCTGCGCCAGCGGCGGGCGCGGCTTGCCGAGCGCATGCAGCTGCAAACAGAGGCCCGCAAC
GAGCTGGAGCGGCGCGTCGAAGAGCGCACTGCCGATCTTGCCCGCGTCAACAGCCGGATCGAAGAGGAGATATCAGAACG
GCGTCTGACCGAGCAGCAGCTCCGTCAGACGCAGGCCGATCTGATCCAGGCGGGCAAGCTTGCCGGCCTGGGGCAGATGT
CGGCGGCCCTTTCGCATGAGTTCAATCAGCCGCTCGCCGCCGCCAAGACCTATACGGACAGCGCCTCCGTTCTCATCGAT
CGCGGCCGGACGGAGGAGGCGCGGGACAATATCCGGCGTATCGGCGGGCTGATCGACCGCATGGCCGCGATCAGCCGGCA
CCTGCGCAATTTCGCCCGCAAACCGAATGAGAAGCTCGGCTCTGTTTCTCTCGACGAAGCGATGCATGACACGCTGGAGA
TCATCGCTTGGCGCCTGAAGGCGGCCGATGCCGAGCTCCGGCTTGATCTGGGAACCCGGCCGCCGGTGGTGCGTGCCGGT
TCGGTGCGTCTGCAGCAAGTGCTGGTGAATGTGATTTCCAATGCGGCCGATGCGGTGGAAGGGCTGGAGGACAGACGGAT
CGAGGTTTCGGCCTTCGAGGAGGCAGGCAAGGTGGTGCTGACAGTGCGGGACCACGGGCCGGGCGTGCCGGCGGCAATCG
CCGAGCGTATCTTCGATCCCTTCTTCACGACCAAGGGTGTCGGCAAGGGGCTCGGTCTCGGGCTGTCGATCTCCTACAAT
ATCATCAAGGATTTCGGCGGCAGCCTTTCCGCGGCCAATCATCCGGAAGGGGGCGCGGTGTTCCATATCGAGCTGCAATC
GGCGGCGGGGCTGATGCCGGAGGCGGCGGAATGA

Upstream 100 bases:

>100_bases
AACTTGAGACCGTCGGGTATGCCTCGCTGGGGAGTGGAGGCGGAAAGGGGAGGAGCCGGACCTGTCTTCGAGATATCACG
CGATAGCGGCGTTGCTGGTG

Downstream 100 bases:

>100_bases
ACGATGCGAAGATCCTGTTGGTCGACGACGAGGAGGAGTTGCGCCGCTCGACGGCGCAGGCGCTGGAGCTGTCCGGCTTC
AGCGTCGAGACCTTTTCGAA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MPLIIFAFFTYGSAIATRAYMGEASAQAGTALRLAVSALSGHLNRYEALPALIADHDDIKELVSAPDDTALRDAANLYLK
EINGLLKSSDIYVVKPDGETIAASNYDGPGSFVGQNFSYRPYFQDAIEGRQARFYALGTTSLKRGYYFAAPIRTGADIRG
VIVFKVDIDMIESSWGGGEYKIFVSDPEGIIFMSGSPEWLYGAILPLTADRIARTEASRRYANARLTELPVTRQRFEPHE
LMTLVGERGSSEYLVLSHYMSAEDWTVNVLMETSSIRTQARTALAAVFLILCIAGLTVAVLRQRRARLAERMQLQTEARN
ELERRVEERTADLARVNSRIEEEISERRLTEQQLRQTQADLIQAGKLAGLGQMSAALSHEFNQPLAAAKTYTDSASVLID
RGRTEEARDNIRRIGGLIDRMAAISRHLRNFARKPNEKLGSVSLDEAMHDTLEIIAWRLKAADAELRLDLGTRPPVVRAG
SVRLQQVLVNVISNAADAVEGLEDRRIEVSAFEEAGKVVLTVRDHGPGVPAAIAERIFDPFFTTKGVGKGLGLGLSISYN
IIKDFGGSLSAANHPEGGAVFHIELQSAAGLMPEAAE

Sequences:

>Translated_597_residues
MPLIIFAFFTYGSAIATRAYMGEASAQAGTALRLAVSALSGHLNRYEALPALIADHDDIKELVSAPDDTALRDAANLYLK
EINGLLKSSDIYVVKPDGETIAASNYDGPGSFVGQNFSYRPYFQDAIEGRQARFYALGTTSLKRGYYFAAPIRTGADIRG
VIVFKVDIDMIESSWGGGEYKIFVSDPEGIIFMSGSPEWLYGAILPLTADRIARTEASRRYANARLTELPVTRQRFEPHE
LMTLVGERGSSEYLVLSHYMSAEDWTVNVLMETSSIRTQARTALAAVFLILCIAGLTVAVLRQRRARLAERMQLQTEARN
ELERRVEERTADLARVNSRIEEEISERRLTEQQLRQTQADLIQAGKLAGLGQMSAALSHEFNQPLAAAKTYTDSASVLID
RGRTEEARDNIRRIGGLIDRMAAISRHLRNFARKPNEKLGSVSLDEAMHDTLEIIAWRLKAADAELRLDLGTRPPVVRAG
SVRLQQVLVNVISNAADAVEGLEDRRIEVSAFEEAGKVVLTVRDHGPGVPAAIAERIFDPFFTTKGVGKGLGLGLSISYN
IIKDFGGSLSAANHPEGGAVFHIELQSAAGLMPEAAE
>Mature_596_residues
PLIIFAFFTYGSAIATRAYMGEASAQAGTALRLAVSALSGHLNRYEALPALIADHDDIKELVSAPDDTALRDAANLYLKE
INGLLKSSDIYVVKPDGETIAASNYDGPGSFVGQNFSYRPYFQDAIEGRQARFYALGTTSLKRGYYFAAPIRTGADIRGV
IVFKVDIDMIESSWGGGEYKIFVSDPEGIIFMSGSPEWLYGAILPLTADRIARTEASRRYANARLTELPVTRQRFEPHEL
MTLVGERGSSEYLVLSHYMSAEDWTVNVLMETSSIRTQARTALAAVFLILCIAGLTVAVLRQRRARLAERMQLQTEARNE
LERRVEERTADLARVNSRIEEEISERRLTEQQLRQTQADLIQAGKLAGLGQMSAALSHEFNQPLAAAKTYTDSASVLIDR
GRTEEARDNIRRIGGLIDRMAAISRHLRNFARKPNEKLGSVSLDEAMHDTLEIIAWRLKAADAELRLDLGTRPPVVRAGS
VRLQQVLVNVISNAADAVEGLEDRRIEVSAFEEAGKVVLTVRDHGPGVPAAIAERIFDPFFTTKGVGKGLGLGLSISYNI
IKDFGGSLSAANHPEGGAVFHIELQSAAGLMPEAAE

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=268, Percent_Identity=29.4776119402985, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790436, Length=240, Percent_Identity=28.75, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI87081816, Length=220, Percent_Identity=30.4545454545455, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1789149, Length=278, Percent_Identity=25.8992805755396, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786912, Length=223, Percent_Identity=28.6995515695067, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 65446; Mature: 65315

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLIIFAFFTYGSAIATRAYMGEASAQAGTALRLAVSALSGHLNRYEALPALIADHDDIK
CCEEEEEHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ELVSAPDDTALRDAANLYLKEINGLLKSSDIYVVKPDGETIAASNYDGPGSFVGQNFSYR
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCC
PYFQDAIEGRQARFYALGTTSLKRGYYFAAPIRTGADIRGVIVFKVDIDMIESSWGGGEY
CCHHHHHCCCCEEEEEEECCHHHCCEEEEECCCCCCCEEEEEEEEEEEEHHHCCCCCCEE
KIFVSDPEGIIFMSGSPEWLYGAILPLTADRIARTEASRRYANARLTELPVTRQRFEPHE
EEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHCCHHH
LMTLVGERGSSEYLVLSHYMSAEDWTVNVLMETSSIRTQARTALAAVFLILCIAGLTVAV
HHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRQRRARLAERMQLQTEARNELERRVEERTADLARVNSRIEEEISERRLTEQQLRQTQAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIQAGKLAGLGQMSAALSHEFNQPLAAAKTYTDSASVLIDRGRTEEARDNIRRIGGLIDR
HHHHCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
MAAISRHLRNFARKPNEKLGSVSLDEAMHDTLEIIAWRLKAADAELRLDLGTRPPVVRAG
HHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCC
SVRLQQVLVNVISNAADAVEGLEDRRIEVSAFEEAGKVVLTVRDHGPGVPAAIAERIFDP
HHHHHHHHHHHHHHHHHHHHCHHHCCEEEEEHHCCCCEEEEEECCCCCCHHHHHHHHHHH
FFTTKGVGKGLGLGLSISYNIIKDFGGSLSAANHPEGGAVFHIELQSAAGLMPEAAE
HHHCCCCCCCCCCCEEEEHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
PLIIFAFFTYGSAIATRAYMGEASAQAGTALRLAVSALSGHLNRYEALPALIADHDDIK
CEEEEEHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ELVSAPDDTALRDAANLYLKEINGLLKSSDIYVVKPDGETIAASNYDGPGSFVGQNFSYR
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCC
PYFQDAIEGRQARFYALGTTSLKRGYYFAAPIRTGADIRGVIVFKVDIDMIESSWGGGEY
CCHHHHHCCCCEEEEEEECCHHHCCEEEEECCCCCCCEEEEEEEEEEEEHHHCCCCCCEE
KIFVSDPEGIIFMSGSPEWLYGAILPLTADRIARTEASRRYANARLTELPVTRQRFEPHE
EEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHCCHHH
LMTLVGERGSSEYLVLSHYMSAEDWTVNVLMETSSIRTQARTALAAVFLILCIAGLTVAV
HHHHHHCCCCCCEEEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRQRRARLAERMQLQTEARNELERRVEERTADLARVNSRIEEEISERRLTEQQLRQTQAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIQAGKLAGLGQMSAALSHEFNQPLAAAKTYTDSASVLIDRGRTEEARDNIRRIGGLIDR
HHHHCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
MAAISRHLRNFARKPNEKLGSVSLDEAMHDTLEIIAWRLKAADAELRLDLGTRPPVVRAG
HHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEECCC
SVRLQQVLVNVISNAADAVEGLEDRRIEVSAFEEAGKVVLTVRDHGPGVPAAIAERIFDP
HHHHHHHHHHHHHHHHHHHHCHHHCCEEEEEHHCCCCEEEEEECCCCCCHHHHHHHHHHH
FFTTKGVGKGLGLGLSISYNIIKDFGGSLSAANHPEGGAVFHIELQSAAGLMPEAAE
HHHCCCCCCCCCCCEEEEHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]