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The map label for this gene is dctD [H]

Identifier: 209550745

GI number: 209550745

Start: 3253448

End: 3254782

Strand: Reverse

Name: dctD [H]

Synonym: Rleg2_3169

Alternate gene names: 209550745

Gene position: 3254782-3253448 (Counterclockwise)

Preceding gene: 209550746

Following gene: 209550744

Centisome position: 71.72

GC content: 65.09

Gene sequence:

>1335_bases
ATGAACGATGCGAAGATCCTGTTGGTCGACGACGAGGAGGAGTTGCGCCGCTCGACGGCGCAGGCGCTGGAGCTGTCCGG
CTTCAGCGTCGAGACCTTTTCGAACGGCGATCATGTATTGGAATTGATCGGCTACAGTTTTCCCGGCGTCGTCGTCAGCG
ACATCCGCATGCCCGGCATGGACGGCATGACGCTGATGCAGAAGATCCGCGAACTCGACCCGGAGGTGCCGGTCATTCTG
GTTACCGGTCACGGCGACGTTCAGCTTGCGGTGAAGGCGATGCGTGAAGGCGCCTATGATTTCATCGAGAAGCCGTTCAC
GCCGGAAATGCTTGCCGGCGTCATCCGCCGGGCGATGGAGCGGCGCGGCCTCGTGCTCGAAAACCGGCTGCTGAAAGCAG
TCGCCGGCAAGCGCGACGATCTCGAAGCGCGGCTGCCGGGGCGCACGCAGGTGATGGTGGATCTGCGCTACCGCATCCGG
GCGATCGGGGCGAGCGATGCCGATACGCTCATTGTCGGCGAGACCGGGGCCGGCAAGGAAGTGGTGGCGCGGGCGCTTCA
CGATATCAGCGCCAGGGCGAGCCGGCCGTTCATCGCCATCAATTGCGCCGCGCTGCCGGCAAACCTGATCGAAAGCGAGC
TCTTCGGCCATGAGGTGGGCGCCTTTCCCGGTGCGGTGAGGCCGCGCTACGGAAAATTCGAACACGGGCGCGGCGGCACC
ATCCTGCTCGACGAAATCGGCTCCATGCCCTTCGACCTGCAGGCGAAGTTCCTGCGGGTGCTGCAGGAGCGGGTGATTTC
CCGGTTGGGATCGAATGAGACGGTGGCGCTCGACGTGCGCTTTATCGCGACGAGCAAGGTAGATCTGGAGGCGGAGGTGG
CGGCTGGGCGCTTCCGCGCCGATCTCTTCTATCGGCTGAACGTTGCAACGCTGCATGTGCCGTCGCTGTCGCAGCGCCGG
GCGGATGTTCCGCTGCTCTTTCTGCAGCTGGTGCGGGAGGCGGCGGCCCGCTACGGCCGCGACGAGGTGGCCGTGCCGCC
CGAGGTGATCTCCGACATCGCCCAGCGCGACTGGCCAGGCAACGTGCGCGAACTCAGGAATGCCGCCGACCGTCTGGTTC
TCGGTCTCGACGATGGCGGGCCGCAGGCCGGGGAGGCGACCGGGCTTGCGGAGCGCGTCGCCGAATTCGAACGGGGGGTC
ATTGCCAGCGCGCTGGTGGCGCATGGCGGCAGTCTCAAGCCGGTTTATGAGACACTCGGCATTTCCCGGAAGACCCTCTA
CGAAAAGATGCAGAAATACGGGCTCGACAAGCGGATGCTGACCTCCGAAGGCTAA

Upstream 100 bases:

>100_bases
ATTTCGGCGGCAGCCTTTCCGCGGCCAATCATCCGGAAGGGGGCGCGGTGTTCCATATCGAGCTGCAATCGGCGGCGGGG
CTGATGCCGGAGGCGGCGGA

Downstream 100 bases:

>100_bases
TTCCGGCCGCTGCCCGTATTCGCGATGGGTGGAAATCCACCCATCGCATCAGCCGTTTGTTTCCAAATCGACCCATGCTG
CATCGCACTGTCGCAAAATC

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MNDAKILLVDDEEELRRSTAQALELSGFSVETFSNGDHVLELIGYSFPGVVVSDIRMPGMDGMTLMQKIRELDPEVPVIL
VTGHGDVQLAVKAMREGAYDFIEKPFTPEMLAGVIRRAMERRGLVLENRLLKAVAGKRDDLEARLPGRTQVMVDLRYRIR
AIGASDADTLIVGETGAGKEVVARALHDISARASRPFIAINCAALPANLIESELFGHEVGAFPGAVRPRYGKFEHGRGGT
ILLDEIGSMPFDLQAKFLRVLQERVISRLGSNETVALDVRFIATSKVDLEAEVAAGRFRADLFYRLNVATLHVPSLSQRR
ADVPLLFLQLVREAAARYGRDEVAVPPEVISDIAQRDWPGNVRELRNAADRLVLGLDDGGPQAGEATGLAERVAEFERGV
IASALVAHGGSLKPVYETLGISRKTLYEKMQKYGLDKRMLTSEG

Sequences:

>Translated_444_residues
MNDAKILLVDDEEELRRSTAQALELSGFSVETFSNGDHVLELIGYSFPGVVVSDIRMPGMDGMTLMQKIRELDPEVPVIL
VTGHGDVQLAVKAMREGAYDFIEKPFTPEMLAGVIRRAMERRGLVLENRLLKAVAGKRDDLEARLPGRTQVMVDLRYRIR
AIGASDADTLIVGETGAGKEVVARALHDISARASRPFIAINCAALPANLIESELFGHEVGAFPGAVRPRYGKFEHGRGGT
ILLDEIGSMPFDLQAKFLRVLQERVISRLGSNETVALDVRFIATSKVDLEAEVAAGRFRADLFYRLNVATLHVPSLSQRR
ADVPLLFLQLVREAAARYGRDEVAVPPEVISDIAQRDWPGNVRELRNAADRLVLGLDDGGPQAGEATGLAERVAEFERGV
IASALVAHGGSLKPVYETLGISRKTLYEKMQKYGLDKRMLTSEG
>Mature_444_residues
MNDAKILLVDDEEELRRSTAQALELSGFSVETFSNGDHVLELIGYSFPGVVVSDIRMPGMDGMTLMQKIRELDPEVPVIL
VTGHGDVQLAVKAMREGAYDFIEKPFTPEMLAGVIRRAMERRGLVLENRLLKAVAGKRDDLEARLPGRTQVMVDLRYRIR
AIGASDADTLIVGETGAGKEVVARALHDISARASRPFIAINCAALPANLIESELFGHEVGAFPGAVRPRYGKFEHGRGGT
ILLDEIGSMPFDLQAKFLRVLQERVISRLGSNETVALDVRFIATSKVDLEAEVAAGRFRADLFYRLNVATLHVPSLSQRR
ADVPLLFLQLVREAAARYGRDEVAVPPEVISDIAQRDWPGNVRELRNAADRLVLGLDDGGPQAGEATGLAERVAEFERGV
IASALVAHGGSLKPVYETLGISRKTLYEKMQKYGLDKRMLTSEG

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=453, Percent_Identity=40.6181015452539, Blast_Score=324, Evalue=7e-90,
Organism=Escherichia coli, GI1790299, Length=474, Percent_Identity=37.5527426160338, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1790437, Length=444, Percent_Identity=37.6126126126126, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=38.5135135135135, Blast_Score=284, Evalue=8e-78,
Organism=Escherichia coli, GI1789087, Length=342, Percent_Identity=40.3508771929825, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI87082117, Length=319, Percent_Identity=41.692789968652, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=38.4375, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87082152, Length=229, Percent_Identity=48.0349344978166, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1786524, Length=315, Percent_Identity=39.0476190476191, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1787583, Length=295, Percent_Identity=37.9661016949153, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87081872, Length=235, Percent_Identity=38.2978723404255, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87081858, Length=330, Percent_Identity=29.6969696969697, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789828, Length=223, Percent_Identity=35.8744394618834, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=31.404958677686, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788394, Length=127, Percent_Identity=30.7086614173228, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082012, Length=152, Percent_Identity=30.9210526315789, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI145693157, Length=120, Percent_Identity=31.6666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=28.099173553719, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=27.7310924369748, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48667; Mature: 48667

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAKILLVDDEEELRRSTAQALELSGFSVETFSNGDHVLELIGYSFPGVVVSDIRMPGM
CCCCEEEEECCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCEEEECCCCCCC
DGMTLMQKIRELDPEVPVILVTGHGDVQLAVKAMREGAYDFIEKPFTPEMLAGVIRRAME
CCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHH
RRGLVLENRLLKAVAGKRDDLEARLPGRTQVMVDLRYRIRAIGASDADTLIVGETGAGKE
HCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEHHEEECCCCCCEEEEECCCCCHH
VVARALHDISARASRPFIAINCAALPANLIESELFGHEVGAFPGAVRPRYGKFEHGRGGT
HHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
ILLDEIGSMPFDLQAKFLRVLQERVISRLGSNETVALDVRFIATSKVDLEAEVAAGRFRA
EEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHE
DLFYRLNVATLHVPSLSQRRADVPLLFLQLVREAAARYGRDEVAVPPEVISDIAQRDWPG
EEEEEEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
NVRELRNAADRLVLGLDDGGPQAGEATGLAERVAEFERGVIASALVAHGGSLKPVYETLG
CHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
ISRKTLYEKMQKYGLDKRMLTSEG
CCHHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure
MNDAKILLVDDEEELRRSTAQALELSGFSVETFSNGDHVLELIGYSFPGVVVSDIRMPGM
CCCCEEEEECCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCEEEECCCCCCC
DGMTLMQKIRELDPEVPVILVTGHGDVQLAVKAMREGAYDFIEKPFTPEMLAGVIRRAME
CCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHH
RRGLVLENRLLKAVAGKRDDLEARLPGRTQVMVDLRYRIRAIGASDADTLIVGETGAGKE
HCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEHHEEECCCCCCEEEEECCCCCHH
VVARALHDISARASRPFIAINCAALPANLIESELFGHEVGAFPGAVRPRYGKFEHGRGGT
HHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
ILLDEIGSMPFDLQAKFLRVLQERVISRLGSNETVALDVRFIATSKVDLEAEVAAGRFRA
EEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHE
DLFYRLNVATLHVPSLSQRRADVPLLFLQLVREAAARYGRDEVAVPPEVISDIAQRDWPG
EEEEEEEEEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
NVRELRNAADRLVLGLDDGGPQAGEATGLAERVAEFERGVIASALVAHGGSLKPVYETLG
CHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
ISRKTLYEKMQKYGLDKRMLTSEG
CCHHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]