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The map label for this gene is emrA [H]

Identifier: 209550680

GI number: 209550680

Start: 3170708

End: 3171931

Strand: Reverse

Name: emrA [H]

Synonym: Rleg2_3104

Alternate gene names: 209550680

Gene position: 3171931-3170708 (Counterclockwise)

Preceding gene: 209550681

Following gene: 209550679

Centisome position: 69.9

GC content: 62.5

Gene sequence:

>1224_bases
ATGGCTGATCAATCCCCCCTCCGCGTCGTTGCCGACGCCAATGCCAAGACACCTGTCGAAGAAGACCAAGCCAAACAGCA
GGAAACGGTAGCCGAAGCGCCTCCATCCAATTCAGCGCCGGCTGCCGCCGTGGCTGCGCCTGGCGGCAACAAGGTCCGCC
GCCGGCGCAGTCTCACGCGGCCGATCCTGTTCGCCCTGCTGCCGGTGGCGCTCGTCGTCGGCGGCTATTATTACGTCAAT
GGCGGTCAGGTGATGTCGACCGACAATGCCTATCTCCAGGCCGATATGGTCGGGCTCACCACCGATGTCTCGGGCATCGT
CGAAAAGATCGACGTGCATGAGAACGAGGCGGTCAAGGCAGGGCAGGTGCTCTTCAGTCTCAGGGCCGACTCCTTCAAGA
TCGCGCTCGATGGCGCCAAGGCGCAGCTCGGCGTGCAGCGCAACCAGATCATGAACCTCAAGGCCAGCTATCAGCAGTCG
CTGGCCGAGATCACGCAGGCGCAAGCCGATCTGCCCTATTACCAGGATCAGTTCGACCGGCAGCAGAACCTCGTCAACAA
TGGCAGCGCGACGCAATCGGCCTATGACGAGGCCAAGCACAATCTCGAGGCTGCGCAGCAGAAGGTGACCGTCGCCAAGG
CGGAAGCTGCAACCACGCTTGCCCAGCTCGGCGGCTCGGCCGACCAGCCGATCGAGGAGAACCCGCTCTACCTGCAGGCC
AAGTCGAATGTCGACAACGCCCAGCGCGAACTCGACCACAGCGTCGTCAAGGCGCCGTTCGACGGCATCGTCACCAATGT
CAACGCGCTGCAGGTCGGCTCCTACCTGCAGGCTTCGCAGCAGGCCTTCTCGCTTGTCGCCACCGATCACCTGTGGATCG
CCGCCAGCCCGAAGGAAACCGAGCTGACCTATGTCAAATCAGGCCAGACGGCGGAAATCTATGTCGACACCTATCCCGGC
GTGATGTGGAAGGGTAAGGTCGAGAGCATCAGCCCGGCCTCCGGCTCCAGCTTCTCGCTGCTGCCGGCGCAGAATACCAC
CGGCAACTGGGTGAAGGTCGTCCAGCGCATTCCGATGCGCGTCAGCATCGAGGATGTGCAAGGCAAGCCGCCGCTTCGTG
TCGGCATGAGCACGGTCGTCGATGTCGAAACCGGCCATGCCCGCGGCCTGCCCGATTTCGTCAATAAACTTCTGGGCAGA
CCCCAGGACAAGGGTCATGAGTAA

Upstream 100 bases:

>100_bases
ACGGCCGAGCAGCGCGAGCAGCTTTTCCACATCCTATCCGTAATGAAGACGAACCTGGTTCAGGCATGCCGGAGCCCGGT
CGACGAGAATGAGACGAACG

Downstream 100 bases:

>100_bases
CGCTTCCGCTTCCCCGGCGAGCCCGGTAGCCAATCGCGGTGCGATCACGGCCTGCGTCATCCTCGCCGTCATCATGCAGG
CGCTGGACACGACGATCGCC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MADQSPLRVVADANAKTPVEEDQAKQQETVAEAPPSNSAPAAAVAAPGGNKVRRRRSLTRPILFALLPVALVVGGYYYVN
GGQVMSTDNAYLQADMVGLTTDVSGIVEKIDVHENEAVKAGQVLFSLRADSFKIALDGAKAQLGVQRNQIMNLKASYQQS
LAEITQAQADLPYYQDQFDRQQNLVNNGSATQSAYDEAKHNLEAAQQKVTVAKAEAATTLAQLGGSADQPIEENPLYLQA
KSNVDNAQRELDHSVVKAPFDGIVTNVNALQVGSYLQASQQAFSLVATDHLWIAASPKETELTYVKSGQTAEIYVDTYPG
VMWKGKVESISPASGSSFSLLPAQNTTGNWVKVVQRIPMRVSIEDVQGKPPLRVGMSTVVDVETGHARGLPDFVNKLLGR
PQDKGHE

Sequences:

>Translated_407_residues
MADQSPLRVVADANAKTPVEEDQAKQQETVAEAPPSNSAPAAAVAAPGGNKVRRRRSLTRPILFALLPVALVVGGYYYVN
GGQVMSTDNAYLQADMVGLTTDVSGIVEKIDVHENEAVKAGQVLFSLRADSFKIALDGAKAQLGVQRNQIMNLKASYQQS
LAEITQAQADLPYYQDQFDRQQNLVNNGSATQSAYDEAKHNLEAAQQKVTVAKAEAATTLAQLGGSADQPIEENPLYLQA
KSNVDNAQRELDHSVVKAPFDGIVTNVNALQVGSYLQASQQAFSLVATDHLWIAASPKETELTYVKSGQTAEIYVDTYPG
VMWKGKVESISPASGSSFSLLPAQNTTGNWVKVVQRIPMRVSIEDVQGKPPLRVGMSTVVDVETGHARGLPDFVNKLLGR
PQDKGHE
>Mature_406_residues
ADQSPLRVVADANAKTPVEEDQAKQQETVAEAPPSNSAPAAAVAAPGGNKVRRRRSLTRPILFALLPVALVVGGYYYVNG
GQVMSTDNAYLQADMVGLTTDVSGIVEKIDVHENEAVKAGQVLFSLRADSFKIALDGAKAQLGVQRNQIMNLKASYQQSL
AEITQAQADLPYYQDQFDRQQNLVNNGSATQSAYDEAKHNLEAAQQKVTVAKAEAATTLAQLGGSADQPIEENPLYLQAK
SNVDNAQRELDHSVVKAPFDGIVTNVNALQVGSYLQASQQAFSLVATDHLWIAASPKETELTYVKSGQTAEIYVDTYPGV
MWKGKVESISPASGSSFSLLPAQNTTGNWVKVVQRIPMRVSIEDVQGKPPLRVGMSTVVDVETGHARGLPDFVNKLLGRP
QDKGHE

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=339, Percent_Identity=31.2684365781711, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788711, Length=341, Percent_Identity=28.7390029325513, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790519, Length=313, Percent_Identity=28.1150159744409, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789637, Length=328, Percent_Identity=25.9146341463415, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081951, Length=319, Percent_Identity=25.3918495297806, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790024, Length=279, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787013, Length=257, Percent_Identity=28.7937743190661, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43784; Mature: 43652

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQSPLRVVADANAKTPVEEDQAKQQETVAEAPPSNSAPAAAVAAPGGNKVRRRRSLTR
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHH
PILFALLPVALVVGGYYYVNGGQVMSTDNAYLQADMVGLTTDVSGIVEKIDVHENEAVKA
HHHHHHHHHHHHHCCEEEECCCEEEECCCCEEEEEHEECHHHHHHHHHHHHCCCCCHHHH
GQVLFSLRADSFKIALDGAKAQLGVQRNQIMNLKASYQQSLAEITQAQADLPYYQDQFDR
HHHHEEECCCCEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QQNLVNNGSATQSAYDEAKHNLEAAQQKVTVAKAEAATTLAQLGGSADQPIEENPLYLQA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
KSNVDNAQRELDHSVVKAPFDGIVTNVNALQVGSYLQASQQAFSLVATDHLWIAASPKET
CCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCEEEEECCCCC
ELTYVKSGQTAEIYVDTYPGVMWKGKVESISPASGSSFSLLPAQNTTGNWVKVVQRIPMR
EEEEEECCCEEEEEEECCCCEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCE
VSIEDVQGKPPLRVGMSTVVDVETGHARGLPDFVNKLLGRPQDKGHE
EEEECCCCCCCCEECCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ADQSPLRVVADANAKTPVEEDQAKQQETVAEAPPSNSAPAAAVAAPGGNKVRRRRSLTR
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHH
PILFALLPVALVVGGYYYVNGGQVMSTDNAYLQADMVGLTTDVSGIVEKIDVHENEAVKA
HHHHHHHHHHHHHCCEEEECCCEEEECCCCEEEEEHEECHHHHHHHHHHHHCCCCCHHHH
GQVLFSLRADSFKIALDGAKAQLGVQRNQIMNLKASYQQSLAEITQAQADLPYYQDQFDR
HHHHEEECCCCEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QQNLVNNGSATQSAYDEAKHNLEAAQQKVTVAKAEAATTLAQLGGSADQPIEENPLYLQA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
KSNVDNAQRELDHSVVKAPFDGIVTNVNALQVGSYLQASQQAFSLVATDHLWIAASPKET
CCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCEEEEECCCCC
ELTYVKSGQTAEIYVDTYPGVMWKGKVESISPASGSSFSLLPAQNTTGNWVKVVQRIPMR
EEEEEECCCEEEEEEECCCCEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCE
VSIEDVQGKPPLRVGMSTVVDVETGHARGLPDFVNKLLGRPQDKGHE
EEEECCCCCCCCEECCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]