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The map label for this gene is braE [H]

Identifier: 209550647

GI number: 209550647

Start: 3133119

End: 3134510

Strand: Reverse

Name: braE [H]

Synonym: Rleg2_3071

Alternate gene names: 209550647

Gene position: 3134510-3133119 (Counterclockwise)

Preceding gene: 209550648

Following gene: 209550646

Centisome position: 69.07

GC content: 58.62

Gene sequence:

>1392_bases
ATGGCAAACATTGAAAATTCTGCAGGCAAGCCCGATGCCGGACTTGTCCGTAAAGGCCTTACCGAAGCCCTTTTCGCCGC
CGTCCTTTCGTTCGGCATGTTCGTCCTGTATGTCGGCCTCAAGACCGACCAGAACATCAACAACGAGCTGATCATCGTCC
AGCGCTGGGGGCTGCTTGCGGTCTTCGTCGCTGTCGCCGCGATTGGCCGCTTCGCCACGGTGGTTTTCCTGCGGCCACAT
CTCGACAGCCGCAAGCTGGCAAAGGCGAGACAGGGCGAACTCGCCATCTCAACCGAGAAGAGCTTCTTCCACCGGCATTT
CCTAAAGATCGCGCTGATCGCGCTGCTGCTTTATCCTATGGTGGTGGTGGCGATCAAAGGTCCGCAGGGGTCGCTGACAT
ATGTCGACAATTTCGGCATCCAGATCTTGATCTATGTGATGCTTGCCTGGGGGCTGAACATCGTCGTCGGCCTCGCCGGT
CTTCTCGATCTCGGTTATGTCGCTTTCTATGCAGTCGGCGCCTATTCCTATGCGCTGCTTTCGAGCCATTTCGGCCTGTC
CTTCTGGGTTCTCCTCCCGCTCTCCGGCATTTTCGCCGCACTTTGGGGCGTTATTCTCGGCTTTCCGGTGCTGCGCCTGC
GCGGCGACTACCTTGCCATCGTGACGCTCGCATTCGGCGAAATCATCCGCCTTGTGCTCATCAACTGGACGGATGTCACC
AAGGGGACTTTCGGTATCTCCAGCATTCCCAAGGTGACGCTTTTCGGCATTCCCTTCGACGCGACGGCCGGCGGCTTCGC
CAAGCTCTTTCACCTGTCGATGTCCTCGGCTTACTACAAGATCTTCCTTTTTTACCTCATTCTGGCGCTCTGCATGCTGA
CCGCCTACGTGACCATCCAGCTGCGCCGCATGCCGATCGGGCGCGCCTGGGAAGCGCTGCGCGAAGACGAGATCGCCTGC
CGCTCGCTCGGCATCAATACGGTGACGACCAAGCTCACGGCCTTTGCGACGGGAGCGATGTTTGCCGGTTTCGCCGGCTC
CTTTTTTGCTGCCCGTCAGGGTTTCGTTTCGCCGGAATCTTTCGTCTTCTTGGAATCGGCTGTGATCCTCGCCATCGTCG
TCCTCGGCGGCATGGGTTCTCTGACCGGGATTGCGATCGCCGCAATCGTCATGGTCGGCGGCACCGAGCTGCTGCGCGAA
ATGGACTTCCTGAAAGTGATTTTCGGGCCGACTTTTACGCCAGAATTGTACCGCATGCTGATCTTCGGCCTCGCCATGGT
CGTGGTCATGCTGTTCAAGCCGCGCGGTTTCGTCGGTTCGCGTGAACCGACCGCCTTCCTCAAGGCGCGCAAGGCAATTT
CCGGAAGCTTCATCAAGGAGGGCCATGGTTGA

Upstream 100 bases:

>100_bases
GCGTACAAGGATGTCGCGACCTTCGCCATCCTCGCTTTCGTGCTGATCTTCAAGCCGACGGGTATCCTCGGACGGCCGGA
AGTCGAGAAGGTATAACGTC

Downstream 100 bases:

>100_bases
TGAGCCCCGTCACCAACACCATGTCGAGCGATACGCTTCTCAAGGTCGAACACCTGTCGATGAAGTTCGGCGGTCTGATG
GCCATCAACGACTTCTCCTT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MANIENSAGKPDAGLVRKGLTEALFAAVLSFGMFVLYVGLKTDQNINNELIIVQRWGLLAVFVAVAAIGRFATVVFLRPH
LDSRKLAKARQGELAISTEKSFFHRHFLKIALIALLLYPMVVVAIKGPQGSLTYVDNFGIQILIYVMLAWGLNIVVGLAG
LLDLGYVAFYAVGAYSYALLSSHFGLSFWVLLPLSGIFAALWGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWTDVT
KGTFGISSIPKVTLFGIPFDATAGGFAKLFHLSMSSAYYKIFLFYLILALCMLTAYVTIQLRRMPIGRAWEALREDEIAC
RSLGINTVTTKLTAFATGAMFAGFAGSFFAARQGFVSPESFVFLESAVILAIVVLGGMGSLTGIAIAAIVMVGGTELLRE
MDFLKVIFGPTFTPELYRMLIFGLAMVVVMLFKPRGFVGSREPTAFLKARKAISGSFIKEGHG

Sequences:

>Translated_463_residues
MANIENSAGKPDAGLVRKGLTEALFAAVLSFGMFVLYVGLKTDQNINNELIIVQRWGLLAVFVAVAAIGRFATVVFLRPH
LDSRKLAKARQGELAISTEKSFFHRHFLKIALIALLLYPMVVVAIKGPQGSLTYVDNFGIQILIYVMLAWGLNIVVGLAG
LLDLGYVAFYAVGAYSYALLSSHFGLSFWVLLPLSGIFAALWGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWTDVT
KGTFGISSIPKVTLFGIPFDATAGGFAKLFHLSMSSAYYKIFLFYLILALCMLTAYVTIQLRRMPIGRAWEALREDEIAC
RSLGINTVTTKLTAFATGAMFAGFAGSFFAARQGFVSPESFVFLESAVILAIVVLGGMGSLTGIAIAAIVMVGGTELLRE
MDFLKVIFGPTFTPELYRMLIFGLAMVVVMLFKPRGFVGSREPTAFLKARKAISGSFIKEGHG
>Mature_462_residues
ANIENSAGKPDAGLVRKGLTEALFAAVLSFGMFVLYVGLKTDQNINNELIIVQRWGLLAVFVAVAAIGRFATVVFLRPHL
DSRKLAKARQGELAISTEKSFFHRHFLKIALIALLLYPMVVVAIKGPQGSLTYVDNFGIQILIYVMLAWGLNIVVGLAGL
LDLGYVAFYAVGAYSYALLSSHFGLSFWVLLPLSGIFAALWGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWTDVTK
GTFGISSIPKVTLFGIPFDATAGGFAKLFHLSMSSAYYKIFLFYLILALCMLTAYVTIQLRRMPIGRAWEALREDEIACR
SLGINTVTTKLTAFATGAMFAGFAGSFFAARQGFVSPESFVFLESAVILAIVVLGGMGSLTGIAIAAIVMVGGTELLREM
DFLKVIFGPTFTPELYRMLIFGLAMVVVMLFKPRGFVGSREPTAFLKARKAISGSFIKEGHG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=311, Percent_Identity=54.3408360128617, Blast_Score=295, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 50331; Mature: 50200

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIENSAGKPDAGLVRKGLTEALFAAVLSFGMFVLYVGLKTDQNINNELIIVQRWGLLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEHHHHHH
VFVAVAAIGRFATVVFLRPHLDSRKLAKARQGELAISTEKSFFHRHFLKIALIALLLYPM
HHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
VVVAIKGPQGSLTYVDNFGIQILIYVMLAWGLNIVVGLAGLLDLGYVAFYAVGAYSYALL
HHEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSHFGLSFWVLLPLSGIFAALWGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWTDVT
HHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
KGTFGISSIPKVTLFGIPFDATAGGFAKLFHLSMSSAYYKIFLFYLILALCMLTAYVTIQ
CCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRRMPIGRAWEALREDEIACRSLGINTVTTKLTAFATGAMFAGFAGSFFAARQGFVSPES
EEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
FVFLESAVILAIVVLGGMGSLTGIAIAAIVMVGGTELLREMDFLKVIFGPTFTPELYRML
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHH
IFGLAMVVVMLFKPRGFVGSREPTAFLKARKAISGSFIKEGHG
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ANIENSAGKPDAGLVRKGLTEALFAAVLSFGMFVLYVGLKTDQNINNELIIVQRWGLLA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEEEHHHHHH
VFVAVAAIGRFATVVFLRPHLDSRKLAKARQGELAISTEKSFFHRHFLKIALIALLLYPM
HHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
VVVAIKGPQGSLTYVDNFGIQILIYVMLAWGLNIVVGLAGLLDLGYVAFYAVGAYSYALL
HHEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSHFGLSFWVLLPLSGIFAALWGVILGFPVLRLRGDYLAIVTLAFGEIIRLVLINWTDVT
HHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
KGTFGISSIPKVTLFGIPFDATAGGFAKLFHLSMSSAYYKIFLFYLILALCMLTAYVTIQ
CCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRRMPIGRAWEALREDEIACRSLGINTVTTKLTAFATGAMFAGFAGSFFAARQGFVSPES
EEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
FVFLESAVILAIVVLGGMGSLTGIAIAAIVMVGGTELLREMDFLKVIFGPTFTPELYRML
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHH
IFGLAMVVVMLFKPRGFVGSREPTAFLKARKAISGSFIKEGHG
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]