BacMap

Definition	Rhizobium leguminosarum bv. trifolii WSM2304 chromosome, complete genome.
Accession	NC_011369
Length	4,537,948

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The map label for this gene is braF [H]

Identifier: 209550646

GI number: 209550646

Start: 3132244

End: 3133119

Strand: Reverse

Name: braF [H]

Synonym: Rleg2_3070

Alternate gene names: 209550646

Gene position: 3133119-3132244 (Counterclockwise)

Preceding gene: 209550647

Following gene: 209550645

Centisome position: 69.04

GC content: 60.05

Gene sequence:

>876_bases
ATGAGCCCCGTCACCAACACCATGTCGAGCGATACGCTTCTCAAGGTCGAACACCTGTCGATGAAGTTCGGCGGTCTGAT
GGCCATCAACGACTTCTCCTTCGAAGCCAAGCGCGGCGATATCACCGCGCTGATCGGGCCGAACGGCGCCGGCAAGACCA
CCGTCTTCAACTGCATCACCGGCTTCTACAAGCCGACGATGGGCATGATCACGCTTAACCAGAAGAGCGGCAAGCAGTAC
CTGCTTGAGCGTCTGCCGGACTTTCGCATCACCAAAGAAGCCAAGGTGGCGCGCACCTTCCAGAACATCCGGCTGTTCTC
CGGCCTGACGGTTCTCGAAAACCTGCTCGTCGCCCAGCACAACAAGCTGATGAAAGCCTCAGGTTATACGATCCTCGGCC
TCATCGGCATCGGTCCCTACAAACGGGAGGCGGCCGCATCGATCGAGCTGGCGCGCCATTGGCTAGAAAAGGCCGATCTG
ATCGACCGCGCCGACGATCCGGCAGGCGATCTGCCCTACGGCGCCCAGCGGCGTCTCGAGATCGCCCGCGCCATGTGCAC
CGGCCCCGAGCTGCTTTGCCTGGACGAACCGGCCGCCGGCCTGAACCCGCGGGAATCGGCAACGCTCAATGCGCTGCTGC
AGAGCATTCGTGCCGAAACCGGAACATCTATCCTGCTCATCGAGCATGACATGTCGGTGGTCATGGAAATCTCCGACCAT
GTCGTCGTGCTCGAATACGGCCAGAAGATTTCTGATGGCACGCCGGATCACGTGAAGAACGATCCGAAGGTCATCGCGGC
CTATCTCGGTGTCGAAGATGAAGAAGTGGAAGAGGTGATCGCAACCGTCGAGCAGCTCGAAGGAGGCGCAAACTGA

Upstream 100 bases:

>100_bases
TGTTCAAGCCGCGCGGTTTCGTCGGTTCGCGTGAACCGACCGCCTTCCTCAAGGCGCGCAAGGCAATTTCCGGAAGCTTC
ATCAAGGAGGGCCATGGTTG

Downstream 100 bases:

>100_bases
TGGGCGATGAAGTAATGACGGGTCAAGCGCTCCTTCAGGTGAATGGCGTCGAAACCTATTACGGCAATATTCGTGCGCTG
GCCGGCATCGATGTCCACGT

Product: ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSPVTNTMSSDTLLKVEHLSMKFGGLMAINDFSFEAKRGDITALIGPNGAGKTTVFNCITGFYKPTMGMITLNQKSGKQY
LLERLPDFRITKEAKVARTFQNIRLFSGLTVLENLLVAQHNKLMKASGYTILGLIGIGPYKREAAASIELARHWLEKADL
IDRADDPAGDLPYGAQRRLEIARAMCTGPELLCLDEPAAGLNPRESATLNALLQSIRAETGTSILLIEHDMSVVMEISDH
VVVLEYGQKISDGTPDHVKNDPKVIAAYLGVEDEEVEEVIATVEQLEGGAN

Sequences:

>Translated_291_residues
MSPVTNTMSSDTLLKVEHLSMKFGGLMAINDFSFEAKRGDITALIGPNGAGKTTVFNCITGFYKPTMGMITLNQKSGKQY
LLERLPDFRITKEAKVARTFQNIRLFSGLTVLENLLVAQHNKLMKASGYTILGLIGIGPYKREAAASIELARHWLEKADL
IDRADDPAGDLPYGAQRRLEIARAMCTGPELLCLDEPAAGLNPRESATLNALLQSIRAETGTSILLIEHDMSVVMEISDH
VVVLEYGQKISDGTPDHVKNDPKVIAAYLGVEDEEVEEVIATVEQLEGGAN
>Mature_290_residues
SPVTNTMSSDTLLKVEHLSMKFGGLMAINDFSFEAKRGDITALIGPNGAGKTTVFNCITGFYKPTMGMITLNQKSGKQYL
LERLPDFRITKEAKVARTFQNIRLFSGLTVLENLLVAQHNKLMKASGYTILGLIGIGPYKREAAASIELARHWLEKADLI
DRADDPAGDLPYGAQRRLEIARAMCTGPELLCLDEPAAGLNPRESATLNALLQSIRAETGTSILLIEHDMSVVMEISDHV
VVLEYGQKISDGTPDHVKNDPKVIAAYLGVEDEEVEEVIATVEQLEGGAN

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI47078218, Length=258, Percent_Identity=29.0697674418605, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI45446740, Length=258, Percent_Identity=29.0697674418605, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI31657092, Length=236, Percent_Identity=29.6610169491525, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI27881501, Length=237, Percent_Identity=26.5822784810127, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI30795238, Length=237, Percent_Identity=26.5822784810127, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI153792144, Length=234, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI105990541, Length=239, Percent_Identity=26.7782426778243, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI21536376, Length=270, Percent_Identity=25.9259259259259, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI46592956, Length=218, Percent_Identity=27.0642201834862, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592978, Length=218, Percent_Identity=27.0642201834862, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592915, Length=218, Percent_Identity=27.0642201834862, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592971, Length=218, Percent_Identity=27.0642201834862, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592964, Length=218, Percent_Identity=27.0642201834862, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592898, Length=218, Percent_Identity=27.0642201834862, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI27477115, Length=216, Percent_Identity=28.2407407407407, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI150417984, Length=244, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI27436953, Length=239, Percent_Identity=28.8702928870293, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI21536378, Length=234, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1789864, Length=261, Percent_Identity=50.1915708812261, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1788225, Length=260, Percent_Identity=28.8461538461538, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787029, Length=255, Percent_Identity=27.4509803921569, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790525, Length=242, Percent_Identity=29.7520661157025, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788761, Length=284, Percent_Identity=28.169014084507, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1789672, Length=258, Percent_Identity=24.4186046511628, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786398, Length=242, Percent_Identity=28.5123966942149, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI87081782, Length=256, Percent_Identity=30.46875, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI87082268, Length=259, Percent_Identity=28.957528957529, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788644, Length=267, Percent_Identity=30.3370786516854, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786872, Length=255, Percent_Identity=26.6666666666667, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI48994997, Length=256, Percent_Identity=27.34375, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081709, Length=265, Percent_Identity=23.3962264150943, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789032, Length=251, Percent_Identity=25.4980079681275, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786563, Length=269, Percent_Identity=27.5092936802974, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788210, Length=261, Percent_Identity=27.2030651340996, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789593, Length=258, Percent_Identity=25.968992248062, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789991, Length=249, Percent_Identity=28.9156626506024, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1787370, Length=250, Percent_Identity=26.8, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1787712, Length=262, Percent_Identity=25.1908396946565, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787089, Length=233, Percent_Identity=27.0386266094421, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48995001, Length=243, Percent_Identity=26.3374485596708, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788450, Length=250, Percent_Identity=24.8, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081791, Length=265, Percent_Identity=23.3962264150943, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790544, Length=267, Percent_Identity=24.3445692883895, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790190, Length=245, Percent_Identity=26.530612244898, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788472, Length=257, Percent_Identity=26.0700389105058, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48994943, Length=257, Percent_Identity=25.6809338521401, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789962, Length=243, Percent_Identity=28.8065843621399, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082267, Length=260, Percent_Identity=25.3846153846154, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787500, Length=274, Percent_Identity=27.007299270073, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787759, Length=270, Percent_Identity=27.4074074074074, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1789873, Length=237, Percent_Identity=26.1603375527426, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786319, Length=240, Percent_Identity=26.25, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790162, Length=259, Percent_Identity=24.3243243243243, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790467, Length=259, Percent_Identity=22.7799227799228, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786253, Length=264, Percent_Identity=26.1363636363636, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081834, Length=210, Percent_Identity=25.7142857142857, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786345, Length=252, Percent_Identity=25.3968253968254, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787164, Length=252, Percent_Identity=25, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786803, Length=251, Percent_Identity=23.1075697211155, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786703, Length=246, Percent_Identity=26.8292682926829, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787143, Length=232, Percent_Identity=28.448275862069, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787792, Length=272, Percent_Identity=25.3676470588235, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788506, Length=253, Percent_Identity=27.6679841897233, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789963, Length=267, Percent_Identity=24.3445692883895, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786981, Length=219, Percent_Identity=32.4200913242009, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17510237, Length=243, Percent_Identity=25.5144032921811, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17565586, Length=238, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=221, Percent_Identity=30.316742081448, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115534520, Length=251, Percent_Identity=23.5059760956175, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17569145, Length=232, Percent_Identity=25, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24648314, Length=254, Percent_Identity=27.1653543307087, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24666092, Length=236, Percent_Identity=29.6610169491525, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI221512771, Length=246, Percent_Identity=27.2357723577236, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920532, Length=218, Percent_Identity=27.9816513761468, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24580930, Length=218, Percent_Identity=27.9816513761468, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI161076682, Length=219, Percent_Identity=27.3972602739726, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161076684, Length=219, Percent_Identity=27.3972602739726, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581617, Length=219, Percent_Identity=27.3972602739726, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24581615, Length=219, Percent_Identity=27.3972602739726, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221500365, Length=243, Percent_Identity=25.5144032921811, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI116007184, Length=243, Percent_Identity=25.5144032921811, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI85724858, Length=220, Percent_Identity=30, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI85724856, Length=220, Percent_Identity=30, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580547, Length=220, Percent_Identity=30, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580551, Length=220, Percent_Identity=30, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580549, Length=220, Percent_Identity=30, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580545, Length=220, Percent_Identity=30, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574744, Length=218, Percent_Identity=27.0642201834862, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 31732; Mature: 31601

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVTNTMSSDTLLKVEHLSMKFGGLMAINDFSFEAKRGDITALIGPNGAGKTTVFNCIT
CCCCCCCCCCCCEEEHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHH
GFYKPTMGMITLNQKSGKQYLLERLPDFRITKEAKVARTFQNIRLFSGLTVLENLLVAQH
HHHCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKLMKASGYTILGLIGIGPYKREAAASIELARHWLEKADLIDRADDPAGDLPYGAQRRLE
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
IARAMCTGPELLCLDEPAAGLNPRESATLNALLQSIRAETGTSILLIEHDMSVVMEISDH
HHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCC
VVVLEYGQKISDGTPDHVKNDPKVIAAYLGVEDEEVEEVIATVEQLEGGAN
EEEEECCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SPVTNTMSSDTLLKVEHLSMKFGGLMAINDFSFEAKRGDITALIGPNGAGKTTVFNCIT
CCCCCCCCCCCEEEHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHH
GFYKPTMGMITLNQKSGKQYLLERLPDFRITKEAKVARTFQNIRLFSGLTVLENLLVAQH
HHHCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKLMKASGYTILGLIGIGPYKREAAASIELARHWLEKADLIDRADDPAGDLPYGAQRRLE
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
IARAMCTGPELLCLDEPAAGLNPRESATLNALLQSIRAETGTSILLIEHDMSVVMEISDH
HHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCC
VVVLEYGQKISDGTPDHVKNDPKVIAAYLGVEDEEVEEVIATVEQLEGGAN
EEEEECCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]