BacMap

Definition	Rhizobium leguminosarum bv. trifolii WSM2304 chromosome, complete genome.
Accession	NC_011369
Length	4,537,948

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The map label for this gene is ygfF [C]

Identifier: 209550482

GI number: 209550482

Start: 2959697

End: 2960446

Strand: Reverse

Name: ygfF [C]

Synonym: Rleg2_2905

Alternate gene names: 209550482

Gene position: 2960446-2959697 (Counterclockwise)

Preceding gene: 209550484

Following gene: 209550477

Centisome position: 65.24

GC content: 65.6

Gene sequence:

>750_bases
ATGGCCAGCTTAGAGTTTTCCGGCAAGACAGCCCTTATCACCGGCGCCGGCAGCGGCATCGGGCGCAGCATCGCGATTGC
CTTCGCCAATGCCGGGGCTAGGGTGGTGTTGGCCGGGCGGCGGCTGGAGGAACTGGAAGAGAGCGCGCGCCTCATCCGTG
AGGGGAGTGGCGAAGCACTCGCCGTTCCAGCCGATGTGACCGATGAGCGGTCGGTCGGCGCCTTGGTCGACCAGGTGGGA
CCGTTGCATGTTGCCGTGAACGCCGCTGGTATCGTGCTCGGCGGCGCAGTCGCCGAGATGGAGGCTGAAGCGTTCGCCCG
TGTCTTTGATGTCAACGTGACCGGGCTTTGGCTGTCGATGAAGCACGAAATCCGCGTCATGAAGGCGACAGGCGGCGGTG
CGATCGTCAACATCGGCTCGAATATCGGCGCGCGGATCGTGCGCCCCAGCATGGGCGCCTATGCCGCCTCAAAGGCTGCC
GTCAGCAGCTTGACGCGGACGGCGGCGCTGGAAGCCATTCCTCATGGCGTCCGCATCAACTGCCTTTGCCCGGGGCCGGT
GGATACACCCCTTTCCATCCGTCCCGGTGAGGATCGAGCGGCGCGTGACGCGCGCGTGGCCGCCACCAATCCTTCCAAAC
GGGTTGCAACGACCGACGAGGTTGCCGCTGCCGCGCTTTGGCTCGCATCCACAGCCGCCTCCTATATCGTCGGTCAGGAT
ATCGTCATCGACGGCGGCGCCTCCGTGTGA

Upstream 100 bases:

>100_bases
CCATGGGTGCCTCATTCTGTGTCGATCAACATATAAATCTCTCGACAGGGTCGGTGCAATCACCTATTTCTGTGCTGATC
GACACATAAATAGGTGAGCC

Downstream 100 bases:

>100_bases
ACATCGACATTGGCGACGATACACCGCCGAAAACAATGTCGCCCCGCTGCCGATATCGTCGATGCTTTCGTCCATGATCT
GAAAAAGCGCCGGGGCTGAG

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MASLEFSGKTALITGAGSGIGRSIAIAFANAGARVVLAGRRLEELEESARLIREGSGEALAVPADVTDERSVGALVDQVG
PLHVAVNAAGIVLGGAVAEMEAEAFARVFDVNVTGLWLSMKHEIRVMKATGGGAIVNIGSNIGARIVRPSMGAYAASKAA
VSSLTRTAALEAIPHGVRINCLCPGPVDTPLSIRPGEDRAARDARVAATNPSKRVATTDEVAAAALWLASTAASYIVGQD
IVIDGGASV

Sequences:

>Translated_249_residues
MASLEFSGKTALITGAGSGIGRSIAIAFANAGARVVLAGRRLEELEESARLIREGSGEALAVPADVTDERSVGALVDQVG
PLHVAVNAAGIVLGGAVAEMEAEAFARVFDVNVTGLWLSMKHEIRVMKATGGGAIVNIGSNIGARIVRPSMGAYAASKAA
VSSLTRTAALEAIPHGVRINCLCPGPVDTPLSIRPGEDRAARDARVAATNPSKRVATTDEVAAAALWLASTAASYIVGQD
IVIDGGASV
>Mature_248_residues
ASLEFSGKTALITGAGSGIGRSIAIAFANAGARVVLAGRRLEELEESARLIREGSGEALAVPADVTDERSVGALVDQVGP
LHVAVNAAGIVLGGAVAEMEAEAFARVFDVNVTGLWLSMKHEIRVMKATGGGAIVNIGSNIGARIVRPSMGAYAASKAAV
SSLTRTAALEAIPHGVRINCLCPGPVDTPLSIRPGEDRAARDARVAATNPSKRVATTDEVAAAALWLASTAASYIVGQDI
VIDGGASV

Specific function: Unknown

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI66933014, Length=256, Percent_Identity=31.25, Blast_Score=110, Evalue=8e-25,
Organism=Homo sapiens, GI10190704, Length=247, Percent_Identity=36.0323886639676, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI32483357, Length=249, Percent_Identity=31.3253012048193, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI15277342, Length=249, Percent_Identity=32.1285140562249, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI19923817, Length=263, Percent_Identity=27.7566539923954, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=30.2040816326531, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI59889578, Length=233, Percent_Identity=31.7596566523605, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI7705925, Length=246, Percent_Identity=32.520325203252, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI5031737, Length=249, Percent_Identity=28.1124497991968, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI126723750, Length=255, Percent_Identity=25.4901960784314, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI40807363, Length=191, Percent_Identity=31.413612565445, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4503817, Length=196, Percent_Identity=30.1020408163265, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI109715829, Length=245, Percent_Identity=27.7551020408163, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4503301, Length=253, Percent_Identity=27.6679841897233, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI210032110, Length=196, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI31542939, Length=211, Percent_Identity=31.2796208530806, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI142976729, Length=198, Percent_Identity=29.2929292929293, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI126723191, Length=185, Percent_Identity=31.3513513513514, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI33667109, Length=195, Percent_Identity=30.2564102564103, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI7706318, Length=194, Percent_Identity=27.8350515463918, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI223718074, Length=188, Percent_Identity=32.9787234042553, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI209862901, Length=200, Percent_Identity=32, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI19923983, Length=200, Percent_Identity=32, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI20149619, Length=195, Percent_Identity=31.2820512820513, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI2367175, Length=246, Percent_Identity=37.3983739837398, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=36.046511627907, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786812, Length=257, Percent_Identity=34.6303501945525, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790717, Length=250, Percent_Identity=30, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1789378, Length=238, Percent_Identity=30.2521008403361, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1788459, Length=244, Percent_Identity=29.9180327868852, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=33.7301587301587, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI2367365, Length=242, Percent_Identity=35.1239669421488, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787335, Length=250, Percent_Identity=29.6, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082160, Length=252, Percent_Identity=26.1904761904762, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1789208, Length=255, Percent_Identity=27.0588235294118, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789057, Length=258, Percent_Identity=27.906976744186, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787526, Length=233, Percent_Identity=27.8969957081545, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=266, Percent_Identity=33.8345864661654, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17531453, Length=267, Percent_Identity=32.9588014981273, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17560220, Length=266, Percent_Identity=34.9624060150376, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17562908, Length=263, Percent_Identity=33.4600760456274, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994600, Length=253, Percent_Identity=33.9920948616601, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17560150, Length=261, Percent_Identity=32.183908045977, Blast_Score=113, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17562990, Length=260, Percent_Identity=31.5384615384615, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17551412, Length=263, Percent_Identity=33.4600760456274, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17560332, Length=267, Percent_Identity=32.2097378277154, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17562904, Length=262, Percent_Identity=32.0610687022901, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17562906, Length=268, Percent_Identity=31.7164179104478, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994604, Length=252, Percent_Identity=32.9365079365079, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17544670, Length=271, Percent_Identity=32.8413284132841, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17538480, Length=268, Percent_Identity=32.4626865671642, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17562910, Length=265, Percent_Identity=29.4339622641509, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI72000259, Length=261, Percent_Identity=31.8007662835249, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17565030, Length=263, Percent_Identity=31.5589353612167, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17555706, Length=252, Percent_Identity=32.9365079365079, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17559104, Length=262, Percent_Identity=30.1526717557252, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17538486, Length=264, Percent_Identity=31.4393939393939, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI115534694, Length=254, Percent_Identity=32.2834645669291, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17536651, Length=259, Percent_Identity=31.6602316602317, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17563726, Length=261, Percent_Identity=31.0344827586207, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17536025, Length=259, Percent_Identity=31.2741312741313, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=29.718875502008, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17561402, Length=250, Percent_Identity=27.6, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560676, Length=246, Percent_Identity=27.6422764227642, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17561272, Length=256, Percent_Identity=32.8125, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193203117, Length=192, Percent_Identity=30.7291666666667, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507613, Length=257, Percent_Identity=31.1284046692607, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32563809, Length=257, Percent_Identity=31.1284046692607, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193203115, Length=188, Percent_Identity=30.3191489361702, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17508895, Length=190, Percent_Identity=27.3684210526316, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17564282, Length=189, Percent_Identity=29.1005291005291, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71997099, Length=217, Percent_Identity=32.258064516129, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=194, Percent_Identity=30.4123711340206, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=257, Percent_Identity=30.3501945525292, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71982365, Length=192, Percent_Identity=28.125, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI212645366, Length=124, Percent_Identity=36.2903225806452, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=205, Percent_Identity=32.6829268292683, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324126, Length=249, Percent_Identity=27.3092369477912, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322861, Length=194, Percent_Identity=27.319587628866, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357041, Length=251, Percent_Identity=37.0517928286853, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI28571526, Length=250, Percent_Identity=37.2, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24644339, Length=252, Percent_Identity=35.7142857142857, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI23397609, Length=252, Percent_Identity=36.5079365079365, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24639444, Length=250, Percent_Identity=34, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=30.6451612903226, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24643142, Length=248, Percent_Identity=31.0483870967742, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24644337, Length=257, Percent_Identity=33.852140077821, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648944, Length=197, Percent_Identity=30.9644670050761, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21358495, Length=197, Percent_Identity=30.4568527918782, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24641232, Length=220, Percent_Identity=31.8181818181818, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364892, Length=168, Percent_Identity=32.7380952380952, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI116007236, Length=195, Percent_Identity=29.7435897435897, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24584752, Length=191, Percent_Identity=32.4607329842932, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281364494, Length=208, Percent_Identity=33.1730769230769, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356301, Length=208, Percent_Identity=25.4807692307692, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17737361, Length=197, Percent_Identity=31.9796954314721, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007238, Length=167, Percent_Identity=29.940119760479, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581726, Length=193, Percent_Identity=25.9067357512953, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24663911, Length=238, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI18859837, Length=195, Percent_Identity=29.2307692307692, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 25248; Mature: 25117

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLEFSGKTALITGAGSGIGRSIAIAFANAGARVVLAGRRLEELEESARLIREGSGEAL
CCCEECCCCEEEEEECCCCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCCEE
AVPADVTDERSVGALVDQVGPLHVAVNAAGIVLGGAVAEMEAEAFARVFDVNVTGLWLSM
EECCCCCCCCHHHHHHHCCCCEEEEEEECEEEECCHHHHHHHHHHHEEEECCEEEEEEEE
KHEIRVMKATGGGAIVNIGSNIGARIVRPSMGAYAASKAAVSSLTRTAALEAIPHGVRIN
EEEEEEEEECCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
CLCPGPVDTPLSIRPGEDRAARDARVAATNPSKRVATTDEVAAAALWLASTAASYIVGQD
EEECCCCCCCCEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCE
IVIDGGASV
EEEECCCCC
>Mature Secondary Structure 
ASLEFSGKTALITGAGSGIGRSIAIAFANAGARVVLAGRRLEELEESARLIREGSGEAL
CCEECCCCEEEEEECCCCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCCEE
AVPADVTDERSVGALVDQVGPLHVAVNAAGIVLGGAVAEMEAEAFARVFDVNVTGLWLSM
EECCCCCCCCHHHHHHHCCCCEEEEEEECEEEECCHHHHHHHHHHHEEEECCEEEEEEEE
KHEIRVMKATGGGAIVNIGSNIGARIVRPSMGAYAASKAAVSSLTRTAALEAIPHGVRIN
EEEEEEEEECCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
CLCPGPVDTPLSIRPGEDRAARDARVAATNPSKRVATTDEVAAAALWLASTAASYIVGQD
EEECCCCCCCCEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCE
IVIDGGASV
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]