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The map label for this gene is neo [H]

Identifier: 209550273

GI number: 209550273

Start: 2731811

End: 2732602

Strand: Reverse

Name: neo [H]

Synonym: Rleg2_2693

Alternate gene names: 209550273

Gene position: 2732602-2731811 (Counterclockwise)

Preceding gene: 209550274

Following gene: 209550272

Centisome position: 60.22

GC content: 63.76

Gene sequence:

>792_bases
ATGTCCCAACAGGATCAACCGCTGCCGGCGGGTGCGCTCGATGCGCGTCTTGCCGGTTACCGGTTCGAACGCGATGCGCT
GGGCCGTTCGGCTGCGAACGTCTTCCGGCTGGAAGCGGGCGGATTGCCGACGCTTTATCTGAAGGTCGAAGAGGCCGGCG
CTTTCGGCGAGCTTGCCGATGAAGCAGCGAGGCTTGACTGGCTCAAGGCTTCCGGTCTGCCCTGTCCCGAGGTCATTGCG
CGGGAAAGCGACGGCGGGCGCAACCGGCTGCTGATCAGCGCCCTGCCAGGTGCTGATCTCGCCAGCGCGTCGACGCTGAC
GCCGCTTCTGCGGGTCGAATTGCTGGCTGCGGCGCTTCTCGAGCTGCATCGACTGCCCATCGCATCCTGCCCCTTCGATC
ACCGGCTGGAGAAGCGCATGGCGGTCGCCAAAGCGCGGATGCAGGCTGGCATCATCGACGAGACCGATTTCGACGAGACG
CGGCTTGGCAAAAGTGCCGAGGCGCTGTTTGCCGAACTCGAAAGCAGAAGGCCGGGCCGCGAGGACCTCGTCGTCACCCA
TGGCGATGCCTGCCTGCCGAATTTCATCGCCTGCGAAGACGGGTTCTCCGGCTATATCGATTGCAGCCGGCTCGGCGTCG
CCGACCGCTATCAGGATATCGCGCTCGCCTGCCGCAGCATCACCTATAATTTCGGCGAGGCGCTGGTGCAGCCCTTCCTC
GATCGTTACGGGCTGACGGCTCTGGATCCGGCAAAGCTCGATTATTTCCAGCTGCTCGACGAATTCTTCTAG

Upstream 100 bases:

>100_bases
GCCGGCCGAAACCGTTCATTGACTTAGCGACGCGAGGTCTGTTGCATAATTTTCCTGCAGTCGGAATGCGATTTCCCGCC
CGTCGGAACGAGGCGCCCAG

Downstream 100 bases:

>100_bases
CCGTTGCGCCATCTCCCTCCGGCTGGCAGAGTGGCCGCAATCGAACCTGCGGACGGACGATGGCGAAGAACCAGTTCAGG
ATGCTGCGCTCGGCGCTGGC

Product: aminoglycoside phosphotransferase

Products: NA

Alternate protein names: APH(3')-II; APH(3')II; Kanamycin kinase, type II; Neomycin-kanamycin phosphotransferase type II [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSQQDQPLPAGALDARLAGYRFERDALGRSAANVFRLEAGGLPTLYLKVEEAGAFGELADEAARLDWLKASGLPCPEVIA
RESDGGRNRLLISALPGADLASASTLTPLLRVELLAAALLELHRLPIASCPFDHRLEKRMAVAKARMQAGIIDETDFDET
RLGKSAEALFAELESRRPGREDLVVTHGDACLPNFIACEDGFSGYIDCSRLGVADRYQDIALACRSITYNFGEALVQPFL
DRYGLTALDPAKLDYFQLLDEFF

Sequences:

>Translated_263_residues
MSQQDQPLPAGALDARLAGYRFERDALGRSAANVFRLEAGGLPTLYLKVEEAGAFGELADEAARLDWLKASGLPCPEVIA
RESDGGRNRLLISALPGADLASASTLTPLLRVELLAAALLELHRLPIASCPFDHRLEKRMAVAKARMQAGIIDETDFDET
RLGKSAEALFAELESRRPGREDLVVTHGDACLPNFIACEDGFSGYIDCSRLGVADRYQDIALACRSITYNFGEALVQPFL
DRYGLTALDPAKLDYFQLLDEFF
>Mature_262_residues
SQQDQPLPAGALDARLAGYRFERDALGRSAANVFRLEAGGLPTLYLKVEEAGAFGELADEAARLDWLKASGLPCPEVIAR
ESDGGRNRLLISALPGADLASASTLTPLLRVELLAAALLELHRLPIASCPFDHRLEKRMAVAKARMQAGIIDETDFDETR
LGKSAEALFAELESRRPGREDLVVTHGDACLPNFIACEDGFSGYIDCSRLGVADRYQDIALACRSITYNFGEALVQPFLD
RYGLTALDPAKLDYFQLLDEFF

Specific function: Resistance to kanamycin, neomycin, paromomycin, ribostamycin, butirosin and gentamicin B [H]

COG id: COG3231

COG function: function code J; Aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.95 [H]

Molecular weight: Translated: 28724; Mature: 28593

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQDQPLPAGALDARLAGYRFERDALGRSAANVFRLEAGGLPTLYLKVEEAGAFGELAD
CCCCCCCCCCCHHHHHHHCCCCCHHHHCHHHHHEEEEECCCCCEEEEEEECCCCHHHHHH
EAARLDWLKASGLPCPEVIARESDGGRNRLLISALPGADLASASTLTPLLRVELLAAALL
HHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
ELHRLPIASCPFDHRLEKRMAVAKARMQAGIIDETDFDETRLGKSAEALFAELESRRPGR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCC
EDLVVTHGDACLPNFIACEDGFSGYIDCSRLGVADRYQDIALACRSITYNFGEALVQPFL
CCEEEECCCCCCCCCEEECCCCCCCEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRYGLTALDPAKLDYFQLLDEFF
HHCCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure 
SQQDQPLPAGALDARLAGYRFERDALGRSAANVFRLEAGGLPTLYLKVEEAGAFGELAD
CCCCCCCCCCHHHHHHHCCCCCHHHHCHHHHHEEEEECCCCCEEEEEEECCCCHHHHHH
EAARLDWLKASGLPCPEVIARESDGGRNRLLISALPGADLASASTLTPLLRVELLAAALL
HHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
ELHRLPIASCPFDHRLEKRMAVAKARMQAGIIDETDFDETRLGKSAEALFAELESRRPGR
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCC
EDLVVTHGDACLPNFIACEDGFSGYIDCSRLGVADRYQDIALACRSITYNFGEALVQPFL
CCEEEECCCCCCCCCEEECCCCCCCEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRYGLTALDPAKLDYFQLLDEFF
HHCCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6295884; 3889831; 12628253 [H]