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The map label for this gene is thyA [H]

Identifier: 209550125

GI number: 209550125

Start: 2574870

End: 2575664

Strand: Reverse

Name: thyA [H]

Synonym: Rleg2_2543

Alternate gene names: 209550125

Gene position: 2575664-2574870 (Counterclockwise)

Preceding gene: 209550129

Following gene: 209550124

Centisome position: 56.76

GC content: 60.38

Gene sequence:

>795_bases
ATGAAGCAATATCTAGATCTCCTCAACCATGTGATGGAAAAGGGCTCCGACCGGGGCGACCGCACCGGCACCGGCACGCG
CTCGGTCTTCGGCTACCAGATGCGCTTCGACCTTCAGGAAGGCTTTCCCGTCACGACGACGAAGAAGCTGCATCTGCGCT
CGATCATCCACGAGCTTTTATGGTTCCTGAAGGGCGAGACGAATATCGGCTATCTCAAGGAGAACGGCGTTTCGATCTGG
GATGAGTGGGCCGACGAAAACGGCGATCTCGGGCCGGTCTACGGCGCCCAGTGGCGCTCCTGGCCGGCGCCGGATGGCGG
CCATATCGATCAGATCGCCAATCTCGTCAAAGGCATCGTCAACAACCCGAACTCGCGCCGCCATATCGTCTCGGCCTGGA
ATCCGGCCGAGGTCGACCAGATGGCGCTGCCGCCCTGCCATTGCCTGTTCCAGTTCTATGTGGCCGATGGCAAGCTCTCC
TGCCAGCTCTATCAGCGCTCGGCCGACATCTTCCTCGGGATTCCGTTCAACATCGCCTCCTATGCGCTTTTGACGATGAT
GGTGGCTGAGGTCACCGGGCTGAAGCCTGGCGATTTCGTCCATACGCTGGGCGACGCCCATCTCTACCACAACCATTTCG
ACCAGGCGAAGCTGCAGCTGGCGCGCCGGCCGAAGCCGCTGCCCTTCATGCGCATCAATCCCGATGTGAAGGACATCTTC
AGCTTCACCTTCGACGATTTCGAGCTGATCGGCTACGAGGCCGATGCCAGCATCAAGGCGCCGATCGCCGTCTGA

Upstream 100 bases:

>100_bases
AAGCGTTATGCGACCCATCAGAAACATTGGGAAAATGCCCGCCGGCGATTGGTTTCCTGCTGCCGGGAGCTTAAATTAGG
CCAAACGGAATCAGCGCGCC

Downstream 100 bases:

>100_bases
TCCGGGACATTCCATGGCCGATATCCGCAGGACCATTATCGCCGCCGTTGCCCGCAACGGCATCATCGGCCGCGATGGAG
ACATGCCCTGGCGGCTGTCG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLDLLNHVMEKGSDRGDRTGTGTRSVFGYQMRFDLQEGFPVTTTKKLHLRSIIHELLWFLKGETNIGYLKENGVSIW
DEWADENGDLGPVYGAQWRSWPAPDGGHIDQIANLVKGIVNNPNSRRHIVSAWNPAEVDQMALPPCHCLFQFYVADGKLS
CQLYQRSADIFLGIPFNIASYALLTMMVAEVTGLKPGDFVHTLGDAHLYHNHFDQAKLQLARRPKPLPFMRINPDVKDIF
SFTFDDFELIGYEADASIKAPIAV

Sequences:

>Translated_264_residues
MKQYLDLLNHVMEKGSDRGDRTGTGTRSVFGYQMRFDLQEGFPVTTTKKLHLRSIIHELLWFLKGETNIGYLKENGVSIW
DEWADENGDLGPVYGAQWRSWPAPDGGHIDQIANLVKGIVNNPNSRRHIVSAWNPAEVDQMALPPCHCLFQFYVADGKLS
CQLYQRSADIFLGIPFNIASYALLTMMVAEVTGLKPGDFVHTLGDAHLYHNHFDQAKLQLARRPKPLPFMRINPDVKDIF
SFTFDDFELIGYEADASIKAPIAV
>Mature_264_residues
MKQYLDLLNHVMEKGSDRGDRTGTGTRSVFGYQMRFDLQEGFPVTTTKKLHLRSIIHELLWFLKGETNIGYLKENGVSIW
DEWADENGDLGPVYGAQWRSWPAPDGGHIDQIANLVKGIVNNPNSRRHIVSAWNPAEVDQMALPPCHCLFQFYVADGKLS
CQLYQRSADIFLGIPFNIASYALLTMMVAEVTGLKPGDFVHTLGDAHLYHNHFDQAKLQLARRPKPLPFMRINPDVKDIF
SFTFDDFELIGYEADASIKAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=49.645390070922, Blast_Score=282, Evalue=2e-76,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=65.1515151515152, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=46.9964664310954, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=40.0684931506849, Blast_Score=203, Evalue=2e-53,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=51.9434628975265, Blast_Score=291, Evalue=4e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 29854; Mature: 29854

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLLNHVMEKGSDRGDRTGTGTRSVFGYQMRFDLQEGFPVTTTKKLHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
WFLKGETNIGYLKENGVSIWDEWADENGDLGPVYGAQWRSWPAPDGGHIDQIANLVKGIV
HHHCCCCCCCEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NNPNSRRHIVSAWNPAEVDQMALPPCHCLFQFYVADGKLSCQLYQRSADIFLGIPFNIAS
CCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YALLTMMVAEVTGLKPGDFVHTLGDAHLYHNHFDQAKLQLARRPKPLPFMRINPDVKDIF
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHH
SFTFDDFELIGYEADASIKAPIAV
HHCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLLNHVMEKGSDRGDRTGTGTRSVFGYQMRFDLQEGFPVTTTKKLHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
WFLKGETNIGYLKENGVSIWDEWADENGDLGPVYGAQWRSWPAPDGGHIDQIANLVKGIV
HHHCCCCCCCEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NNPNSRRHIVSAWNPAEVDQMALPPCHCLFQFYVADGKLSCQLYQRSADIFLGIPFNIAS
CCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YALLTMMVAEVTGLKPGDFVHTLGDAHLYHNHFDQAKLQLARRPKPLPFMRINPDVKDIF
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHH
SFTFDDFELIGYEADASIKAPIAV
HHCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA