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The map label for this gene is dhfrIII [H]

Identifier: 209550124

GI number: 209550124

Start: 2574338

End: 2574856

Strand: Reverse

Name: dhfrIII [H]

Synonym: Rleg2_2542

Alternate gene names: 209550124

Gene position: 2574856-2574338 (Counterclockwise)

Preceding gene: 209550125

Following gene: 209550123

Centisome position: 56.74

GC content: 65.7

Gene sequence:

>519_bases
ATGGCCGATATCCGCAGGACCATTATCGCCGCCGTTGCCCGCAACGGCATCATCGGCCGCGATGGAGACATGCCCTGGCG
GCTGTCGAGCGATCTCAAGCGCTTCAAGGCGCTGACATTGGGCAGGCCTGTCGTGATGGGCCGCAAGACCTATGATTCGA
TCGGCAAGCCGCTGCCGGGGCGGCCGAACGTCGTCATCTCCAGGCAGGCGGCGATAGACCATCCCGATGTTTCCATGGCG
CATTCGCTGCCGGAAGCGATGACGGCGGCCGAAAGGCTGGCGCGCGAAAGCGGCGTCGACGAGGTCTGCATCATCGGCGG
CGGTCAGGTCTATGCCCAGGCGATCGGCCTTGCCGACCGGATGTGCATCACCCATGTCGAGGCCGACCTCGACGGAGACG
CATCGTTTCCCGCGATCGATCCCGACATCTGGCGGGCAGGGGAGCCTATCGCGGTTCCGGCCGGCGAGAAGGACAGTCAC
CCCACGCGCTTCGTCGTCTACGAGCGCCGACACGCCTGA

Upstream 100 bases:

>100_bases
AAGGACATCTTCAGCTTCACCTTCGACGATTTCGAGCTGATCGGCTACGAGGCCGATGCCAGCATCAAGGCGCCGATCGC
CGTCTGATCCGGGACATTCC

Downstream 100 bases:

>100_bases
AAATGAACTATTTTCCAATTAAATTGACCAAGTTTCTCACGTTGCGTTGAAAGCCGCTGCGGCGATACTTATAACGGTCC
CAACGCATCCGCCGGTCAGC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MADIRRTIIAAVARNGIIGRDGDMPWRLSSDLKRFKALTLGRPVVMGRKTYDSIGKPLPGRPNVVISRQAAIDHPDVSMA
HSLPEAMTAAERLARESGVDEVCIIGGGQVYAQAIGLADRMCITHVEADLDGDASFPAIDPDIWRAGEPIAVPAGEKDSH
PTRFVVYERRHA

Sequences:

>Translated_172_residues
MADIRRTIIAAVARNGIIGRDGDMPWRLSSDLKRFKALTLGRPVVMGRKTYDSIGKPLPGRPNVVISRQAAIDHPDVSMA
HSLPEAMTAAERLARESGVDEVCIIGGGQVYAQAIGLADRMCITHVEADLDGDASFPAIDPDIWRAGEPIAVPAGEKDSH
PTRFVVYERRHA
>Mature_171_residues
ADIRRTIIAAVARNGIIGRDGDMPWRLSSDLKRFKALTLGRPVVMGRKTYDSIGKPLPGRPNVVISRQAAIDHPDVSMAH
SLPEAMTAAERLARESGVDEVCIIGGGQVYAQAIGLADRMCITHVEADLDGDASFPAIDPDIWRAGEPIAVPAGEKDSHP
TRFVVYERRHA

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=180, Percent_Identity=30, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1786233, Length=164, Percent_Identity=40.2439024390244, Blast_Score=127, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17505899, Length=149, Percent_Identity=29.5302013422819, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324810, Length=148, Percent_Identity=34.4594594594595, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI116008026, Length=175, Percent_Identity=34.8571428571429, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24647458, Length=175, Percent_Identity=34.8571428571429, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18649; Mature: 18517

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIRRTIIAAVARNGIIGRDGDMPWRLSSDLKRFKALTLGRPVVMGRKTYDSIGKPLPG
CCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHCCCCCC
RPNVVISRQAAIDHPDVSMAHSLPEAMTAAERLARESGVDEVCIIGGGQVYAQAIGLADR
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHH
MCITHVEADLDGDASFPAIDPDIWRAGEPIAVPAGEKDSHPTRFVVYERRHA
HHHHHHHCCCCCCCCCCCCCHHHHCCCCCEEECCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
ADIRRTIIAAVARNGIIGRDGDMPWRLSSDLKRFKALTLGRPVVMGRKTYDSIGKPLPG
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHCCCCCC
RPNVVISRQAAIDHPDVSMAHSLPEAMTAAERLARESGVDEVCIIGGGQVYAQAIGLADR
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHH
MCITHVEADLDGDASFPAIDPDIWRAGEPIAVPAGEKDSHPTRFVVYERRHA
HHHHHHHCCCCCCCCCCCCCHHHHCCCCCEEECCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]