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The map label for this gene is cobW [H]

Identifier: 209549725

GI number: 209549725

Start: 2191639

End: 2192688

Strand: Reverse

Name: cobW [H]

Synonym: Rleg2_2132

Alternate gene names: 209549725

Gene position: 2192688-2191639 (Counterclockwise)

Preceding gene: 209549726

Following gene: 209549724

Centisome position: 48.32

GC content: 63.14

Gene sequence:

>1050_bases
ATGAACCAGCAAAAGATTCCCGCAACCGTCATCACCGGCTTCCTCGGCGCCGGCAAGACGACGATGATCCGCAATCTGCT
GACCAATGCCGGCGGCAAAAAGATCGCGCTAATCATCAATGAATTCGGCGATCTCGGTGTCGACGGCGAGGTGCTGAAGG
GCTGCGGCGCGGAGAACTGCAGCGAGGACGATATTATCGAGCTGACCAATGGCTGCATCTGCTGCACCGTTGCCGACGAT
TTCATTCCGACGATGACGAAGCTTCTGGAGCGCGAACAGCGGCCCGACCATATCGTCATCGAAACCTCGGGCCTCGCTCT
GCCGCAGCCGCTGGTCGCCGCCTTCAACTGGCCTGACATTCGCACCCAGGTGACCGTCGACGGCGTCATCACGGTGGTCG
ACAGCGCGGCTGTCGCCGCCGGCCGTTTCGCCGATGACCATGATGCCGTCGAGGCGGCGCGCAGCGAGGATCAATCGCTC
GATCACGAGAGCCCGATCGAGGAACTGTTCGAGGACCAGCTCACCTGCGCCGATCTCATCGTGCTCAACAAGACCGACCT
GATCGACGTGGCCGGCCTTGGCCGGGTGCGCGACGAGGTCGCCGCCCGCACCCAGCGCAAGCCGGTGATGATCGAGGCGA
AAAACGGCGAGGTGCCGGCCGGCATCCTGCTCGGCCTCGGCATCGGCACGGAGGACGATATCGCCAACCGCAAGTCCCAT
CACGAACTGGAGCATGAGGACGGCACGCCGCACGATCATGACGAATTCGACAGCTTCGTCGTTGATCTCGGCCCCGTTGC
CGATCCGGCCGGCTTCGTCGAGGCGCTGAAGAGCATCATATCAGCCCATGACGTGCTGCGCCTCAAGGGCTTCGTCGATG
TTTCGGGCAAGCCGATGCGTCTGCAGCTCCAGGCCGTCGGCAGCCGTATCGACCACTATTTCGATCGCGCCTGGGCGCCT
GGGGAAAAGCGCGGCACGCGCCTCGTGGTCATTGGTCTGCATGAGATGGATCAGGGTGCGGTGAAGGCGGCGATCGAGGC
ACTGGCGTAA

Upstream 100 bases:

>100_bases
CGCGATCATGCCGGCCGGCTCCACCAGATGATCGCGGCAAAGGCCGCCGAAGTCTATTTTATCGCGGCAGGCCTGCCGCT
GAAAATGAAGGGTTAAATCC

Downstream 100 bases:

>100_bases
GCCCATGCATCTGCTTCTGGCCCAACAGGGAACGATCAGCGACGGCGAAGAGGCAATCGATCTCGGTCAGACGCCGGGCG
ATATCCTGTTCCTGTCGGCC

Product: cobalamin biosynthesis protein CobW

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MNQQKIPATVITGFLGAGKTTMIRNLLTNAGGKKIALIINEFGDLGVDGEVLKGCGAENCSEDDIIELTNGCICCTVADD
FIPTMTKLLEREQRPDHIVIETSGLALPQPLVAAFNWPDIRTQVTVDGVITVVDSAAVAAGRFADDHDAVEAARSEDQSL
DHESPIEELFEDQLTCADLIVLNKTDLIDVAGLGRVRDEVAARTQRKPVMIEAKNGEVPAGILLGLGIGTEDDIANRKSH
HELEHEDGTPHDHDEFDSFVVDLGPVADPAGFVEALKSIISAHDVLRLKGFVDVSGKPMRLQLQAVGSRIDHYFDRAWAP
GEKRGTRLVVIGLHEMDQGAVKAAIEALA

Sequences:

>Translated_349_residues
MNQQKIPATVITGFLGAGKTTMIRNLLTNAGGKKIALIINEFGDLGVDGEVLKGCGAENCSEDDIIELTNGCICCTVADD
FIPTMTKLLEREQRPDHIVIETSGLALPQPLVAAFNWPDIRTQVTVDGVITVVDSAAVAAGRFADDHDAVEAARSEDQSL
DHESPIEELFEDQLTCADLIVLNKTDLIDVAGLGRVRDEVAARTQRKPVMIEAKNGEVPAGILLGLGIGTEDDIANRKSH
HELEHEDGTPHDHDEFDSFVVDLGPVADPAGFVEALKSIISAHDVLRLKGFVDVSGKPMRLQLQAVGSRIDHYFDRAWAP
GEKRGTRLVVIGLHEMDQGAVKAAIEALA
>Mature_349_residues
MNQQKIPATVITGFLGAGKTTMIRNLLTNAGGKKIALIINEFGDLGVDGEVLKGCGAENCSEDDIIELTNGCICCTVADD
FIPTMTKLLEREQRPDHIVIETSGLALPQPLVAAFNWPDIRTQVTVDGVITVVDSAAVAAGRFADDHDAVEAARSEDQSL
DHESPIEELFEDQLTCADLIVLNKTDLIDVAGLGRVRDEVAARTQRKPVMIEAKNGEVPAGILLGLGIGTEDDIANRKSH
HELEHEDGTPHDHDEFDSFVVDLGPVADPAGFVEALKSIISAHDVLRLKGFVDVSGKPMRLQLQAVGSRIDHYFDRAWAP
GEKRGTRLVVIGLHEMDQGAVKAAIEALA

Specific function: Might be involved in cobalt reduction leading to cobalt(I) corrinoids [H]

COG id: COG0523

COG function: function code R; Putative GTPases (G3E family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cobW C-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI33469141, Length=343, Percent_Identity=27.9883381924198, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI126722884, Length=345, Percent_Identity=27.8260869565217, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI146231952, Length=345, Percent_Identity=28.1159420289855, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI223941779, Length=345, Percent_Identity=28.4057971014493, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI148727351, Length=345, Percent_Identity=27.8260869565217, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI223941776, Length=334, Percent_Identity=26.3473053892216, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI119120938, Length=136, Percent_Identity=39.7058823529412, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI87082430, Length=351, Percent_Identity=29.9145299145299, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1788499, Length=181, Percent_Identity=32.0441988950276, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324356, Length=192, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012824
- InterPro:   IPR003495
- InterPro:   IPR011629 [H]

Pfam domain/function: PF02492 cobW; PF07683 CobW_C [H]

EC number: NA

Molecular weight: Translated: 37561; Mature: 37561

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQKIPATVITGFLGAGKTTMIRNLLTNAGGKKIALIINEFGDLGVDGEVLKGCGAENC
CCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCCCCC
SEDDIIELTNGCICCTVADDFIPTMTKLLEREQRPDHIVIETSGLALPQPLVAAFNWPDI
CCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCCC
RTQVTVDGVITVVDSAAVAAGRFADDHDAVEAARSEDQSLDHESPIEELFEDQLTCADLI
CEEEEECCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHEE
VLNKTDLIDVAGLGRVRDEVAARTQRKPVMIEAKNGEVPAGILLGLGIGTEDDIANRKSH
EECCCCEEEHHCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCHHHHCCHHH
HELEHEDGTPHDHDEFDSFVVDLGPVADPAGFVEALKSIISAHDVLRLKGFVDVSGKPMR
CCCCCCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCCEE
LQLQAVGSRIDHYFDRAWAPGEKRGTRLVVIGLHEMDQGAVKAAIEALA
EEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNQQKIPATVITGFLGAGKTTMIRNLLTNAGGKKIALIINEFGDLGVDGEVLKGCGAENC
CCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCCCCC
SEDDIIELTNGCICCTVADDFIPTMTKLLEREQRPDHIVIETSGLALPQPLVAAFNWPDI
CCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCCC
RTQVTVDGVITVVDSAAVAAGRFADDHDAVEAARSEDQSLDHESPIEELFEDQLTCADLI
CEEEEECCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHEE
VLNKTDLIDVAGLGRVRDEVAARTQRKPVMIEAKNGEVPAGILLGLGIGTEDDIANRKSH
EECCCCEEEHHCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCHHHHCCHHH
HELEHEDGTPHDHDEFDSFVVDLGPVADPAGFVEALKSIISAHDVLRLKGFVDVSGKPMR
CCCCCCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCCEE
LQLQAVGSRIDHYFDRAWAPGEKRGTRLVVIGLHEMDQGAVKAAIEALA
EEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1655697 [H]