Click here to switch to the map view.

The map label for this gene is cobD [H]

Identifier: 209549714

GI number: 209549714

Start: 2179011

End: 2179991

Strand: Reverse

Name: cobD [H]

Synonym: Rleg2_2121

Alternate gene names: 209549714

Gene position: 2179991-2179011 (Counterclockwise)

Preceding gene: 209549715

Following gene: 209549713

Centisome position: 48.04

GC content: 65.24

Gene sequence:

>981_bases
ATGACGATCGACGAAAACCTTCTTGTGTTGCTTCTGGCGCTGCTGCTCGACCGGATCGCCGGGGATCCGCAATGGCTGTG
GTCACGCGTGCCGCATCCCGTCGTCATGTTCGGCGCGGCGATCTCCTATGCCGACCAGCAGCTCAATCCTTCAAGCCTTA
CCGGCTCCCAGCGCCGTATGAACGGCGTCGCCGCGATCCTGGCGCTGCTGCTGCTGTCGATTGCCGCTGGCTTCGTGTTC
GACCGGTTCTTCGCGCTCTTCGGCCTTGTCGGCCTCGTGCTGGAAGCCGGTCTGGTGGCGATCTTCCTGGCGCAGAAGAG
CCTTGCCGATCATGTCGCGGCCGTCGCCACCGCCTTGCGGGGCGAAGGGCTCGCCGGCGGCCGGAATGCCGTCTCCCGCA
TCGTCGGCCGCGATCCCGAAACGCTCGACGAGCCGGCCGTCTGCCGCGCTGCGATCGAAAGCCTTGCGGAAAATTTCTCC
GACGGCGTCGTGGCGCCGGCTCTCTGCTATGCCGTCTTCGGCCTGCCGGGTCTGCTTGCCTACAAGATGCTGAACACTGC
GGATTCGATGATCGGCCACAGATCGGAAAAATACATCGATTTCGGTTGGGCTGCGGCCCGGCTCGACGATATCGTCAACT
GGCCGGCCGCGCGGCTTTCCATCCTGCTGATCGCCGCCGGGGCCTGGATCCGGCGCGGCATAAGCCCCTGCCGCGAGGCG
ATCCGCGTGGCGATGCGGGACGGCGGCCTGCACCGATCGCCGAATTCCGGCAGGCCCGAGGCGGCCATGGCCGGCGCTCT
CAACGTCCAGCTCGCCGGCCCGCGCATCTATGGCGGTGTGATCGTGACGGAGCCGATGATCAACAATGCCGGCCGCGACA
TTGCCACCGCCGGCGACATTGAGGACGGCGTGTCGGTGTTCTATGCCAGCTGCATGGTGCTCACCGGTCTGATCTTCGGA
TTGTTCTTGTGTTTTCTGTAG

Upstream 100 bases:

>100_bases
TCCGCAAGTTCGATTATGCGCCGGATTGGCTGCGGTTCGGCTTGACGCCCGATCCGGCGGCGGACAGGCGGCTTGGCGAA
GCCCTTCAGGGATTTGAGCG

Downstream 100 bases:

>100_bases
GGTGCCACGCACCGAGTCCACCCCGGTGGACAGCTCAGTCTCAAAGGTTTATGCGAAGCGCCTCATATCTCATCAGACCG
GAAGCAGAGCGATGACAGTT

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTIDENLLVLLLALLLDRIAGDPQWLWSRVPHPVVMFGAAISYADQQLNPSSLTGSQRRMNGVAAILALLLLSIAAGFVF
DRFFALFGLVGLVLEAGLVAIFLAQKSLADHVAAVATALRGEGLAGGRNAVSRIVGRDPETLDEPAVCRAAIESLAENFS
DGVVAPALCYAVFGLPGLLAYKMLNTADSMIGHRSEKYIDFGWAAARLDDIVNWPAARLSILLIAAGAWIRRGISPCREA
IRVAMRDGGLHRSPNSGRPEAAMAGALNVQLAGPRIYGGVIVTEPMINNAGRDIATAGDIEDGVSVFYASCMVLTGLIFG
LFLCFL

Sequences:

>Translated_326_residues
MTIDENLLVLLLALLLDRIAGDPQWLWSRVPHPVVMFGAAISYADQQLNPSSLTGSQRRMNGVAAILALLLLSIAAGFVF
DRFFALFGLVGLVLEAGLVAIFLAQKSLADHVAAVATALRGEGLAGGRNAVSRIVGRDPETLDEPAVCRAAIESLAENFS
DGVVAPALCYAVFGLPGLLAYKMLNTADSMIGHRSEKYIDFGWAAARLDDIVNWPAARLSILLIAAGAWIRRGISPCREA
IRVAMRDGGLHRSPNSGRPEAAMAGALNVQLAGPRIYGGVIVTEPMINNAGRDIATAGDIEDGVSVFYASCMVLTGLIFG
LFLCFL
>Mature_325_residues
TIDENLLVLLLALLLDRIAGDPQWLWSRVPHPVVMFGAAISYADQQLNPSSLTGSQRRMNGVAAILALLLLSIAAGFVFD
RFFALFGLVGLVLEAGLVAIFLAQKSLADHVAAVATALRGEGLAGGRNAVSRIVGRDPETLDEPAVCRAAIESLAENFSD
GVVAPALCYAVFGLPGLLAYKMLNTADSMIGHRSEKYIDFGWAAARLDDIVNWPAARLSILLIAAGAWIRRGISPCREAI
RVAMRDGGLHRSPNSGRPEAAMAGALNVQLAGPRIYGGVIVTEPMINNAGRDIATAGDIEDGVSVFYASCMVLTGLIFGL
FLCFL

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34576; Mature: 34445

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDENLLVLLLALLLDRIAGDPQWLWSRVPHPVVMFGAAISYADQQLNPSSLTGSQRRM
CCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHCCCHHHHH
NGVAAILALLLLSIAAGFVFDRFFALFGLVGLVLEAGLVAIFLAQKSLADHVAAVATALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEGLAGGRNAVSRIVGRDPETLDEPAVCRAAIESLAENFSDGVVAPALCYAVFGLPGLLA
CCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
YKMLNTADSMIGHRSEKYIDFGWAAARLDDIVNWPAARLSILLIAAGAWIRRGISPCREA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHH
IRVAMRDGGLHRSPNSGRPEAAMAGALNVQLAGPRIYGGVIVTEPMINNAGRDIATAGDI
HHHHHHCCCCCCCCCCCCCHHHHHCCCEEEECCCEEECCEEEECHHHCCCCCCCCCCCCC
EDGVSVFYASCMVLTGLIFGLFLCFL
HHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TIDENLLVLLLALLLDRIAGDPQWLWSRVPHPVVMFGAAISYADQQLNPSSLTGSQRRM
CCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHCCCHHHHH
NGVAAILALLLLSIAAGFVFDRFFALFGLVGLVLEAGLVAIFLAQKSLADHVAAVATALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEGLAGGRNAVSRIVGRDPETLDEPAVCRAAIESLAENFSDGVVAPALCYAVFGLPGLLA
CCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
YKMLNTADSMIGHRSEKYIDFGWAAARLDDIVNWPAARLSILLIAAGAWIRRGISPCREA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHH
IRVAMRDGGLHRSPNSGRPEAAMAGALNVQLAGPRIYGGVIVTEPMINNAGRDIATAGDI
HHHHHHCCCCCCCCCCCCCHHHHHCCCEEEECCCEEECCEEEECHHHCCCCCCCCCCCCC
EDGVSVFYASCMVLTGLIFGLFLCFL
HHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2211520 [H]