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The map label for this gene is ramA [H]

Identifier: 209549699

GI number: 209549699

Start: 2166389

End: 2167123

Strand: Reverse

Name: ramA [H]

Synonym: Rleg2_2106

Alternate gene names: 209549699

Gene position: 2167123-2166389 (Counterclockwise)

Preceding gene: 209549700

Following gene: 209549698

Centisome position: 47.76

GC content: 58.91

Gene sequence:

>735_bases
GTGAGAAGCATCCGGATAGCCGCTGCGCAGACGCCGGAATTCCGGGAAAATATCGAGGCGGCCTTGGATTATGCCGTTGG
AGTCGCAGCCCAAGCCGAGACCGATGGCGTGGCCTTGCTGCTCTTTCCCGAAGGGTTTCTCCAGGGCTACCTGACCGATG
AACCATTGGCACGTCGCGTTGCGCTCGATCTCGCATCGACCGCGTTTGAGGCTGTCCTGCATCGGCTGCCGAAGTCGGGA
CCGATGCTGGTCATGGGGCTGATCGAGATCGATGACGGACGATTGTTCAACACAGCTGTTGTCGTCGAGCACGGCGTTCT
TCTCGGCCGATACCGCAAAGCCCACCTGTTGCGCGGCGAACAGGCCTTCGAGGCGGGAACGGAGAGCCCGCTCTTTGCGA
TCGGTGATCTGCGCTTTGGGATAAACATCTGCTACGACACCAACTTTCCCGAAGCGGCGGCAAAGCTCGCGGCGCTGGGC
GCATCGCTGATCTTATGTTGCTCCAACAATATGATGCCACGAGATAAGGCGGAAATATTCAAGGAGCAGCACAATGTCGT
TCGCGGTGAAAGATGCCGCGAAACCGGTCTCTGGATGATATCGTCCGACGTGACAGGCGAGCGCTATGGGCGCGTCGCCT
GGGGGCCGACGGCGGTTCTGAACTCGGAAGGGCAGGTCGTGGCGCAATTGCCTCTGGAAAAACCCGGCTTACTCATTTTC
GATTATCCTGCTTAG

Upstream 100 bases:

>100_bases
AGGCTTGCCGAGAAGAGATCGAGGAAAGCCCGGCCGTCGCGCCGGGCTTTTTCGTGGCTTCGGCCGCTCAGGCGGTGAGG
CGTTCGAAAGGGAGATCGAC

Downstream 100 bases:

>100_bases
CATCGGCAAGCGATCATGCATGAGGAGTTGACCGAGATGCTCATCAGATACGAAACGCCGGCAGATATCGATGCGATCCA
CGATCTGACCTCGGTCGCGT

Product: nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRSIRIAAAQTPEFRENIEAALDYAVGVAAQAETDGVALLLFPEGFLQGYLTDEPLARRVALDLASTAFEAVLHRLPKSG
PMLVMGLIEIDDGRLFNTAVVVEHGVLLGRYRKAHLLRGEQAFEAGTESPLFAIGDLRFGINICYDTNFPEAAAKLAALG
ASLILCCSNNMMPRDKAEIFKEQHNVVRGERCRETGLWMISSDVTGERYGRVAWGPTAVLNSEGQVVAQLPLEKPGLLIF
DYPA

Sequences:

>Translated_244_residues
MRSIRIAAAQTPEFRENIEAALDYAVGVAAQAETDGVALLLFPEGFLQGYLTDEPLARRVALDLASTAFEAVLHRLPKSG
PMLVMGLIEIDDGRLFNTAVVVEHGVLLGRYRKAHLLRGEQAFEAGTESPLFAIGDLRFGINICYDTNFPEAAAKLAALG
ASLILCCSNNMMPRDKAEIFKEQHNVVRGERCRETGLWMISSDVTGERYGRVAWGPTAVLNSEGQVVAQLPLEKPGLLIF
DYPA
>Mature_244_residues
MRSIRIAAAQTPEFRENIEAALDYAVGVAAQAETDGVALLLFPEGFLQGYLTDEPLARRVALDLASTAFEAVLHRLPKSG
PMLVMGLIEIDDGRLFNTAVVVEHGVLLGRYRKAHLLRGEQAFEAGTESPLFAIGDLRFGINICYDTNFPEAAAKLAALG
ASLILCCSNNMMPRDKAEIFKEQHNVVRGERCRETGLWMISSDVTGERYGRVAWGPTAVLNSEGQVVAQLPLEKPGLLIF
DYPA

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 26619; Mature: 26619

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIRIAAAQTPEFRENIEAALDYAVGVAAQAETDGVALLLFPEGFLQGYLTDEPLARRV
CCCEEEEECCCCHHHHHHHHHHHHHHCCEEECCCCCEEEEEECHHHHCCCCCCCHHHHHH
ALDLASTAFEAVLHRLPKSGPMLVMGLIEIDDGRLFNTAVVVEHGVLLGRYRKAHLLRGE
HHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCC
QAFEAGTESPLFAIGDLRFGINICYDTNFPEAAAKLAALGASLILCCSNNMMPRDKAEIF
HHHHCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHH
KEQHNVVRGERCRETGLWMISSDVTGERYGRVAWGPTAVLNSEGQVVAQLPLEKPGLLIF
HHHHHHHCCHHHHHCCCEEEECCCCCCCCCCEEECCEEEECCCCCEEEECCCCCCCEEEE
DYPA
ECCC
>Mature Secondary Structure
MRSIRIAAAQTPEFRENIEAALDYAVGVAAQAETDGVALLLFPEGFLQGYLTDEPLARRV
CCCEEEEECCCCHHHHHHHHHHHHHHCCEEECCCCCEEEEEECHHHHCCCCCCCHHHHHH
ALDLASTAFEAVLHRLPKSGPMLVMGLIEIDDGRLFNTAVVVEHGVLLGRYRKAHLLRGE
HHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCC
QAFEAGTESPLFAIGDLRFGINICYDTNFPEAAAKLAALGASLILCCSNNMMPRDKAEIF
HHHHCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHEEEEECCCCCCCHHHHHH
KEQHNVVRGERCRETGLWMISSDVTGERYGRVAWGPTAVLNSEGQVVAQLPLEKPGLLIF
HHHHHHHCCHHHHHCCCEEEECCCCCCCCCCEEECCEEEECCCCCEEEECCCCCCCEEEE
DYPA
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA