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The map label for this gene is yfhM [H]

Identifier: 209549645

GI number: 209549645

Start: 2114923

End: 2115957

Strand: Reverse

Name: yfhM [H]

Synonym: Rleg2_2052

Alternate gene names: 209549645

Gene position: 2115957-2114923 (Counterclockwise)

Preceding gene: 209549648

Following gene: 209549644

Centisome position: 46.63

GC content: 61.16

Gene sequence:

>1035_bases
ATGTCAGAGCAAATCAACCATCACCGCCGCCGCTTCTTCGGCATGACGGCAATCGCCCTTGCGGCCGTCGAATTCGGCGT
GGCCGGGACAGCCGTCGCCCAGTCGGCGCTGCCCGCCGTAAAGGCCGGAACCAATACGTCTTTCGAAGCGCTGAAGCAGG
TGAAGGCGGGCGTGCTCGATATTGGTTATGCCGAGGCCGGCAGCGCGGATGGTCCCGTTGTTCTGCTGCTGCATGGCTGG
CCCTACGATATTTATAGTTTCGTCGATGTTGCGCCGCTGCTGGCCTCGGCGGGTTACAGGGTGATCGTCCCCTATCTGCG
TGGTTACGGCACGACCCGCTTCCTGGACGACCAGACACCGCGCAACGGTCAGCCGTCGGCGCTGGCTGCCGATATGATCG
CGCTGCTCGATGCGCTCGATATCGAGAAGGCAGTGATTGCCGGCTATGACTGGGGCGGGAGGACGGCCAACATTATGGCG
GCGCTATGGCCGGAGCGCTGCAAGGCGATGGTCTCGGTGAGCGGCTACCTGATCGGCAGCCAGGAGGCTAATTTGAAGCC
GCTGCCGCCGAAGGCGGAACTGGCCTGGTGGTATCAGTTCTATTTTGCAACCGAACGTGGGCGGCTGGGTTACGAGAGCA
ATACGCATGATTTCGCAAAGCTCATCTGGCAGACGGCTTCGCCGAAGTGGAATTTCGACGATGCGACTTTCGACAGGTCG
GCGGCTGCCTTCGACAATCCCGACCATGTCGCGATCGTCATTCACAATTATCGCTGGCGCCTGGGGCTTGTCGAAGGCGA
GGCCAAGTACGATGCCTATGAGAAGACGCTTGCCGCATTGCCGATGATCTCCGTGCCGACGATCACCATGGAGGGGGATG
CAAACGGTGCGCCGCATCCGGAGCCATCCGCCTATGCCGGAAAATTCTCCGGCAAATACGAGCATCGCACGATTAATGGC
GGCATAGGCCACAACCTGCCGCAGGAAGCGCCGCAGGCCTTTGCGCAGGCGGTCATCGACGTCGACCGCTTCTGA

Upstream 100 bases:

>100_bases
CCGGAAGCTACATCAGCATTCAATAATGATTGCTCATGACATATCGCTGATACAAGTGGAACGTCTTTTGAGGACGTGAT
TCATTCGACAGGAGAGACCG

Downstream 100 bases:

>100_bases
TCGATGAGACCGGCCGCAGAGCCTCGTTGCCTGGGCCGGCGGAATATGCGTAGGTCGCATCAGCCGCCTCGATATCGAGG
AGGAGGGCGCGGCCGCTTCT

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSEQINHHRRRFFGMTAIALAAVEFGVAGTAVAQSALPAVKAGTNTSFEALKQVKAGVLDIGYAEAGSADGPVVLLLHGW
PYDIYSFVDVAPLLASAGYRVIVPYLRGYGTTRFLDDQTPRNGQPSALAADMIALLDALDIEKAVIAGYDWGGRTANIMA
ALWPERCKAMVSVSGYLIGSQEANLKPLPPKAELAWWYQFYFATERGRLGYESNTHDFAKLIWQTASPKWNFDDATFDRS
AAAFDNPDHVAIVIHNYRWRLGLVEGEAKYDAYEKTLAALPMISVPTITMEGDANGAPHPEPSAYAGKFSGKYEHRTING
GIGHNLPQEAPQAFAQAVIDVDRF

Sequences:

>Translated_344_residues
MSEQINHHRRRFFGMTAIALAAVEFGVAGTAVAQSALPAVKAGTNTSFEALKQVKAGVLDIGYAEAGSADGPVVLLLHGW
PYDIYSFVDVAPLLASAGYRVIVPYLRGYGTTRFLDDQTPRNGQPSALAADMIALLDALDIEKAVIAGYDWGGRTANIMA
ALWPERCKAMVSVSGYLIGSQEANLKPLPPKAELAWWYQFYFATERGRLGYESNTHDFAKLIWQTASPKWNFDDATFDRS
AAAFDNPDHVAIVIHNYRWRLGLVEGEAKYDAYEKTLAALPMISVPTITMEGDANGAPHPEPSAYAGKFSGKYEHRTING
GIGHNLPQEAPQAFAQAVIDVDRF
>Mature_343_residues
SEQINHHRRRFFGMTAIALAAVEFGVAGTAVAQSALPAVKAGTNTSFEALKQVKAGVLDIGYAEAGSADGPVVLLLHGWP
YDIYSFVDVAPLLASAGYRVIVPYLRGYGTTRFLDDQTPRNGQPSALAADMIALLDALDIEKAVIAGYDWGGRTANIMAA
LWPERCKAMVSVSGYLIGSQEANLKPLPPKAELAWWYQFYFATERGRLGYESNTHDFAKLIWQTASPKWNFDDATFDRSA
AAFDNPDHVAIVIHNYRWRLGLVEGEAKYDAYEKTLAALPMISVPTITMEGDANGAPHPEPSAYAGKFSGKYEHRTINGG
IGHNLPQEAPQAFAQAVIDVDRF

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=146, Percent_Identity=36.3013698630137, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324392, Length=125, Percent_Identity=36.8, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 37501; Mature: 37369

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQINHHRRRFFGMTAIALAAVEFGVAGTAVAQSALPAVKAGTNTSFEALKQVKAGVLD
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHHCEEE
IGYAEAGSADGPVVLLLHGWPYDIYSFVDVAPLLASAGYRVIVPYLRGYGTTRFLDDQTP
ECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCEEECCCCC
RNGQPSALAADMIALLDALDIEKAVIAGYDWGGRTANIMAALWPERCKAMVSVSGYLIGS
CCCCCHHHHHHHHHHHHHHCCCHHEEECCCCCCCCHHHHHHHCHHHHHHHHHEEEEEEEC
QEANLKPLPPKAELAWWYQFYFATERGRLGYESNTHDFAKLIWQTASPKWNFDDATFDRS
CCCCCCCCCCCCHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
AAAFDNPDHVAIVIHNYRWRLGLVEGEAKYDAYEKTLAALPMISVPTITMEGDANGAPHP
CCCCCCCCEEEEEEECCEEEEEEEECCHHHHHHHHHHHHCCCEECCEEEEECCCCCCCCC
EPSAYAGKFSGKYEHRTINGGIGHNLPQEAPQAFAQAVIDVDRF
CCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEQINHHRRRFFGMTAIALAAVEFGVAGTAVAQSALPAVKAGTNTSFEALKQVKAGVLD
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHHCEEE
IGYAEAGSADGPVVLLLHGWPYDIYSFVDVAPLLASAGYRVIVPYLRGYGTTRFLDDQTP
ECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCEEECCCCC
RNGQPSALAADMIALLDALDIEKAVIAGYDWGGRTANIMAALWPERCKAMVSVSGYLIGS
CCCCCHHHHHHHHHHHHHHCCCHHEEECCCCCCCCHHHHHHHCHHHHHHHHHEEEEEEEC
QEANLKPLPPKAELAWWYQFYFATERGRLGYESNTHDFAKLIWQTASPKWNFDDATFDRS
CCCCCCCCCCCCHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
AAAFDNPDHVAIVIHNYRWRLGLVEGEAKYDAYEKTLAALPMISVPTITMEGDANGAPHP
CCCCCCCCEEEEEEECCEEEEEEEECCHHHHHHHHHHHHCCCEECCEEEEECCCCCCCCC
EPSAYAGKFSGKYEHRTINGGIGHNLPQEAPQAFAQAVIDVDRF
CCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]