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The map label for this gene is hsrA [H]

Identifier: 209549590

GI number: 209549590

Start: 2054202

End: 2055605

Strand: Direct

Name: hsrA [H]

Synonym: Rleg2_1997

Alternate gene names: 209549590

Gene position: 2054202-2055605 (Clockwise)

Preceding gene: 209549588

Following gene: 209549591

Centisome position: 45.27

GC content: 64.03

Gene sequence:

>1404_bases
ATGATCCTTGCGGTCGCCCTGTTCATGGAACAGATGGATTCGACCGTCATCGCCACCAGCCTGCCGGCGATCGCCGCCGA
TATCGGCACCTCGCCGATCGCGCTGAAGCTTGCTGTCACCAGCTATCTCGTGGCGCTGGCGATCTTCATCCCGATCAGCG
GCTGGATGTCCGATCGTTTCGGCGCGCGCAACATCTTCCGTTTGGCGATCTTCGTCTTCATGATCGGCTCGGTCGCCTGC
GCCTTTTCCAATTCGATCGCGGCCTTCGTCGTCTCGCGCCTGATCCAGGGCGCCGGCGGCTCGATGATGACGCCGGTCAG
CCGCCTGCTGCTGGTCCGGGGAACGCCGCGCCACGAACTCGTCGATGCCATGGCCTGGCTCACCATTCCCGCACTGATCG
GCCCGATCATGGGGCCGCCGATCGGCGGTTTCCTCACCACCTATCTCAGCTGGCACTGGATCTTCTGGATCAATGTCCCG
ATCGGCGTGCTCGGCATCTTCCTCGTCACGCGTTTCCTGCCGGCGGTCGAGCCGCGCAGTCCGCGGCCGATGGATTTTCC
GGGCTTCTTCCTTTGCGGCATCGGCTTTTCCGGCTTCGTTTTCGGCCTGTCGGTGATCAGCCTGCCGGCCGTGCCCGTCA
TCTACGGCTATGTCACGGTGGCCGTCGGCATCCTTGCCGGCCTCCTCTATCTCCTGCATGCCCGCCGCACGCCCTATCCG
CTGCTCGATCCGAAAATGTTCCGTTACCCGATGTTCCGGGCGGCGATCCTCGGCGCCTCGAATTTCCGCATGGGGCTCGG
CGCCCTGCCCTTTCTGATGCCGCTGATGCTCCAGCTCGGCTTCGGCCTGACGCCGCTGCAATCGGGCTCAGTCACCTTCG
TCAGCGCGCTCGGCTCCATGGGGTCGAAATTTGCCGCCTCGCGCACCTTCAATGCCTTCGGCTTCCGTACCGTCATATCG
ATCACCACCTTGCTGGCGGCAATCTGCCTCGGCATCAACGGCTTCTTCACCGCTGAAACGCCGCTGCCGCTGATCATGGC
CTGCCTGCTGATCGGCGGCCTTTTCCGCTCAATGGCCTTTTCGGGCGTCAACGCCATGGCCTTCGGCGATGTCGATGATG
CCGACAGCAGCCAGGCGACCGCGATCAACGCCGTTGCCCAGCGTATCTCCATGGCGATGGGCGTGGCGATCGCCGGCGGC
ATCCTCGAAATTTCGAGCAGCTTTCACGGAGGCAGGCTGCTGGTCTCGGATTTTCACACCGCCTTCTTCAGCGTCTCGGC
GATTTCGGCGCTCGCCTGCATCACCTTCCTGCGCCTGCCGCGCGATGCCGGCGCCGAATTGACCACGCGCCGCCGCAAAC
GCCACCAGCCCGAGCCCGAAGAGGCGATCGCGGAAAACAGCTGA

Upstream 100 bases:

>100_bases
TGCGCATCGCTGCCGTAGAAACGAACAGATCCCAACCGACGCCCGTGGCGCCGCAACTTTCCTCTTTGCACCCCGGTTGC
CCGTGCCCCGCCTGACACCC

Downstream 100 bases:

>100_bases
TCAAGCCAAATCGCCCGGCCGGGACGGCGGCGAAATCAGGCGCGTGTGCTCCGGCATCCCATTCTACTTTATGATTTATT
CACACATCAAAGACACACTG

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MILAVALFMEQMDSTVIATSLPAIAADIGTSPIALKLAVTSYLVALAIFIPISGWMSDRFGARNIFRLAIFVFMIGSVAC
AFSNSIAAFVVSRLIQGAGGSMMTPVSRLLLVRGTPRHELVDAMAWLTIPALIGPIMGPPIGGFLTTYLSWHWIFWINVP
IGVLGIFLVTRFLPAVEPRSPRPMDFPGFFLCGIGFSGFVFGLSVISLPAVPVIYGYVTVAVGILAGLLYLLHARRTPYP
LLDPKMFRYPMFRAAILGASNFRMGLGALPFLMPLMLQLGFGLTPLQSGSVTFVSALGSMGSKFAASRTFNAFGFRTVIS
ITTLLAAICLGINGFFTAETPLPLIMACLLIGGLFRSMAFSGVNAMAFGDVDDADSSQATAINAVAQRISMAMGVAIAGG
ILEISSSFHGGRLLVSDFHTAFFSVSAISALACITFLRLPRDAGAELTTRRRKRHQPEPEEAIAENS

Sequences:

>Translated_467_residues
MILAVALFMEQMDSTVIATSLPAIAADIGTSPIALKLAVTSYLVALAIFIPISGWMSDRFGARNIFRLAIFVFMIGSVAC
AFSNSIAAFVVSRLIQGAGGSMMTPVSRLLLVRGTPRHELVDAMAWLTIPALIGPIMGPPIGGFLTTYLSWHWIFWINVP
IGVLGIFLVTRFLPAVEPRSPRPMDFPGFFLCGIGFSGFVFGLSVISLPAVPVIYGYVTVAVGILAGLLYLLHARRTPYP
LLDPKMFRYPMFRAAILGASNFRMGLGALPFLMPLMLQLGFGLTPLQSGSVTFVSALGSMGSKFAASRTFNAFGFRTVIS
ITTLLAAICLGINGFFTAETPLPLIMACLLIGGLFRSMAFSGVNAMAFGDVDDADSSQATAINAVAQRISMAMGVAIAGG
ILEISSSFHGGRLLVSDFHTAFFSVSAISALACITFLRLPRDAGAELTTRRRKRHQPEPEEAIAENS
>Mature_467_residues
MILAVALFMEQMDSTVIATSLPAIAADIGTSPIALKLAVTSYLVALAIFIPISGWMSDRFGARNIFRLAIFVFMIGSVAC
AFSNSIAAFVVSRLIQGAGGSMMTPVSRLLLVRGTPRHELVDAMAWLTIPALIGPIMGPPIGGFLTTYLSWHWIFWINVP
IGVLGIFLVTRFLPAVEPRSPRPMDFPGFFLCGIGFSGFVFGLSVISLPAVPVIYGYVTVAVGILAGLLYLLHARRTPYP
LLDPKMFRYPMFRAAILGASNFRMGLGALPFLMPLMLQLGFGLTPLQSGSVTFVSALGSMGSKFAASRTFNAFGFRTVIS
ITTLLAAICLGINGFFTAETPLPLIMACLLIGGLFRSMAFSGVNAMAFGDVDDADSSQATAINAVAQRISMAMGVAIAGG
ILEISSSFHGGRLLVSDFHTAFFSVSAISALACITFLRLPRDAGAELTTRRRKRHQPEPEEAIAENS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=470, Percent_Identity=36.5957446808511, Blast_Score=314, Evalue=9e-87,
Organism=Escherichia coli, GI1788392, Length=459, Percent_Identity=34.4226579520697, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788710, Length=440, Percent_Identity=25, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI87081983, Length=264, Percent_Identity=27.6515151515151, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789042, Length=293, Percent_Identity=29.3515358361775, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788509, Length=137, Percent_Identity=30.6569343065693, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790146, Length=167, Percent_Identity=25.748502994012, Blast_Score=62, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=146, Percent_Identity=31.5068493150685, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=170, Percent_Identity=30, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=388, Percent_Identity=24.2268041237113, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49858; Mature: 49858

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILAVALFMEQMDSTVIATSLPAIAADIGTSPIALKLAVTSYLVALAIFIPISGWMSDRF
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCC
GARNIFRLAIFVFMIGSVACAFSNSIAAFVVSRLIQGAGGSMMTPVSRLLLVRGTPRHEL
CHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHH
VDAMAWLTIPALIGPIMGPPIGGFLTTYLSWHWIFWINVPIGVLGIFLVTRFLPAVEPRS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHCCCCCCC
PRPMDFPGFFLCGIGFSGFVFGLSVISLPAVPVIYGYVTVAVGILAGLLYLLHARRTPYP
CCCCCCCCHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LLDPKMFRYPMFRAAILGASNFRMGLGALPFLMPLMLQLGFGLTPLQSGSVTFVSALGSM
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
GSKFAASRTFNAFGFRTVISITTLLAAICLGINGFFTAETPLPLIMACLLIGGLFRSMAF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHH
SGVNAMAFGDVDDADSSQATAINAVAQRISMAMGVAIAGGILEISSSFHGGRLLVSDFHT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
AFFSVSAISALACITFLRLPRDAGAELTTRRRKRHQPEPEEAIAENS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHCCCC
>Mature Secondary Structure
MILAVALFMEQMDSTVIATSLPAIAADIGTSPIALKLAVTSYLVALAIFIPISGWMSDRF
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCC
GARNIFRLAIFVFMIGSVACAFSNSIAAFVVSRLIQGAGGSMMTPVSRLLLVRGTPRHEL
CHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHH
VDAMAWLTIPALIGPIMGPPIGGFLTTYLSWHWIFWINVPIGVLGIFLVTRFLPAVEPRS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHCCCCCCC
PRPMDFPGFFLCGIGFSGFVFGLSVISLPAVPVIYGYVTVAVGILAGLLYLLHARRTPYP
CCCCCCCCHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LLDPKMFRYPMFRAAILGASNFRMGLGALPFLMPLMLQLGFGLTPLQSGSVTFVSALGSM
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
GSKFAASRTFNAFGFRTVISITTLLAAICLGINGFFTAETPLPLIMACLLIGGLFRSMAF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHH
SGVNAMAFGDVDDADSSQATAINAVAQRISMAMGVAIAGGILEISSSFHGGRLLVSDFHT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
AFFSVSAISALACITFLRLPRDAGAELTTRRRKRHQPEPEEAIAENS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]