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The map label for this gene is bcr [H]

Identifier: 209549534

GI number: 209549534

Start: 1983737

End: 1984984

Strand: Direct

Name: bcr [H]

Synonym: Rleg2_1940

Alternate gene names: 209549534

Gene position: 1983737-1984984 (Clockwise)

Preceding gene: 209549533

Following gene: 209549535

Centisome position: 43.71

GC content: 60.42

Gene sequence:

>1248_bases
GTGACAGATTCATCACAAGCCGTGTCCGCGGGCCGCCTGCCCATGGGCAAGCGAGAATTTATCGCGCTCGCCGCCTTCCT
GATGGCCGTCAACTCTCTGGCGATCGACATCATGCTGCCGGCGTTGCAGCAGATCGGCGCGAGCCTCGGCGTCGAGAGCG
AAAATCATCGCCAGTTCGTCGTTTCCACCTATCTGCTCGGCTTCGGTTGTGCGCAGTTGTTCTACGGGCCGCTGTCTGAC
CGCTTCGGCCGCCGCACGCCGCTTTTGATCGGTCTCGTCATCTACATTCTCTCCGCCATCAGTATCGTCTTCGTCCCCAC
CTTCGCAGGCTTGCTTGCCCTGCGCTTTGTCCAGGGCATCGGTTCGGCGGCAACCCGCGTCATCACCGTCTCCATTGTCC
GCGACATCTATGGCGGCAGGCAGATGGCCGAAGTCATGTCGCTGATCATGATGGTCTTCATGATCGTGCCGGTGATTGCA
CCAGGCACCGGCCAGATCGTGATGTTCTTCGGCAACTGGCACCTGATCTTCCTTTTCATCGCCGCCATGGCGATTGCAAT
CGCCGTCTGGGCTTACATCCGCCTGCCGGAAACCCTGCATCCACAGAATGTCCGCCCCTTCACCGCCCGCTCGATCTTCG
GCGCCTTCAAGCTGGTGCTGACCAATCGCATCGCGCTTTGCTATACCATCGCCAGCACCTTCATCTTCGGCGCGCTGTTC
GGCTTCATCAATTCGGCCCAGCAGGTCTATGTCGGCATTTACGATCTCGGCGTCTATTTCCCCTTCGCCTTTGCCGGCGT
GGCGATCTTCATGTCGCTGTCGTCGTTTTTCAACTCACGCTTCGTCGGCAAACTCGGCATGCGTCGCCTGTCGCACGGCT
CGCTCATCGGTTTCATCACCATCAACACCATCTGGCTGATCGTCCAGATGGCGAGTTCCGAGCCGATGCCTTTCGCTCTG
TTCATCTCCTTCTTCGGCCTCGCCATGTTCCAGTTCGGCTGGATCGGCTCGAACTTCAATTCGCTCGCCATGGAGCCGCT
CGGTCATGTCGCCGGCACCGCCTCCTCCGTCCTCGGCTTCATGAGCACGGTCGGCGGCGCCCTCATCGGCGCCGGCATCG
GCCAGGCTTTCGACGGCACTGCGCTGCCGATGGTCGCCGGTTATTTCATCGTGTCGATCATCGGGCTGATCTTCGTGCTG
ATCGCCGAAAAGGGCCGCCTCTTCCAACAGCAGAACCCGGCCGTCTGA

Upstream 100 bases:

>100_bases
CTGACCGGTCACCGGGCGTCCGCCCGGTTTCCAAATCTGTAATCTTGAGACCGAGGCGCTGCGGCTAGCAGCGCCTTAGC
CTGTTTATGGGAATTTCATT

Downstream 100 bases:

>100_bases
ACGGCCCACCTCCTCTGAACGGCCTACCTCCTCCGCATCCAGGATTTCACCACATGACGCCGCCGCATAAACCGCACCAG
GAAAACCAGGGCTCCCGTCG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTDSSQAVSAGRLPMGKREFIALAAFLMAVNSLAIDIMLPALQQIGASLGVESENHRQFVVSTYLLGFGCAQLFYGPLSD
RFGRRTPLLIGLVIYILSAISIVFVPTFAGLLALRFVQGIGSAATRVITVSIVRDIYGGRQMAEVMSLIMMVFMIVPVIA
PGTGQIVMFFGNWHLIFLFIAAMAIAIAVWAYIRLPETLHPQNVRPFTARSIFGAFKLVLTNRIALCYTIASTFIFGALF
GFINSAQQVYVGIYDLGVYFPFAFAGVAIFMSLSSFFNSRFVGKLGMRRLSHGSLIGFITINTIWLIVQMASSEPMPFAL
FISFFGLAMFQFGWIGSNFNSLAMEPLGHVAGTASSVLGFMSTVGGALIGAGIGQAFDGTALPMVAGYFIVSIIGLIFVL
IAEKGRLFQQQNPAV

Sequences:

>Translated_415_residues
MTDSSQAVSAGRLPMGKREFIALAAFLMAVNSLAIDIMLPALQQIGASLGVESENHRQFVVSTYLLGFGCAQLFYGPLSD
RFGRRTPLLIGLVIYILSAISIVFVPTFAGLLALRFVQGIGSAATRVITVSIVRDIYGGRQMAEVMSLIMMVFMIVPVIA
PGTGQIVMFFGNWHLIFLFIAAMAIAIAVWAYIRLPETLHPQNVRPFTARSIFGAFKLVLTNRIALCYTIASTFIFGALF
GFINSAQQVYVGIYDLGVYFPFAFAGVAIFMSLSSFFNSRFVGKLGMRRLSHGSLIGFITINTIWLIVQMASSEPMPFAL
FISFFGLAMFQFGWIGSNFNSLAMEPLGHVAGTASSVLGFMSTVGGALIGAGIGQAFDGTALPMVAGYFIVSIIGLIFVL
IAEKGRLFQQQNPAV
>Mature_414_residues
TDSSQAVSAGRLPMGKREFIALAAFLMAVNSLAIDIMLPALQQIGASLGVESENHRQFVVSTYLLGFGCAQLFYGPLSDR
FGRRTPLLIGLVIYILSAISIVFVPTFAGLLALRFVQGIGSAATRVITVSIVRDIYGGRQMAEVMSLIMMVFMIVPVIAP
GTGQIVMFFGNWHLIFLFIAAMAIAIAVWAYIRLPETLHPQNVRPFTARSIFGAFKLVLTNRIALCYTIASTFIFGALFG
FINSAQQVYVGIYDLGVYFPFAFAGVAIFMSLSSFFNSRFVGKLGMRRLSHGSLIGFITINTIWLIVQMASSEPMPFALF
ISFFGLAMFQFGWIGSNFNSLAMEPLGHVAGTASSVLGFMSTVGGALIGAGIGQAFDGTALPMVAGYFIVSIIGLIFVLI
AEKGRLFQQQNPAV

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=378, Percent_Identity=31.7460317460317, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI48994889, Length=162, Percent_Identity=38.2716049382716, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082312, Length=357, Percent_Identity=29.1316526610644, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790146, Length=382, Percent_Identity=28.5340314136126, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790794, Length=178, Percent_Identity=31.4606741573034, Blast_Score=86, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324264, Length=168, Percent_Identity=27.9761904761905, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44865; Mature: 44734

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSSQAVSAGRLPMGKREFIALAAFLMAVNSLAIDIMLPALQQIGASLGVESENHRQFV
CCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VSTYLLGFGCAQLFYGPLSDRFGRRTPLLIGLVIYILSAISIVFVPTFAGLLALRFVQGI
HHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSAATRVITVSIVRDIYGGRQMAEVMSLIMMVFMIVPVIAPGTGQIVMFFGNWHLIFLFI
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHH
AAMAIAIAVWAYIRLPETLHPQNVRPFTARSIFGAFKLVLTNRIALCYTIASTFIFGALF
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFINSAQQVYVGIYDLGVYFPFAFAGVAIFMSLSSFFNSRFVGKLGMRRLSHGSLIGFIT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
INTIWLIVQMASSEPMPFALFISFFGLAMFQFGWIGSNFNSLAMEPLGHVAGTASSVLGF
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
MSTVGGALIGAGIGQAFDGTALPMVAGYFIVSIIGLIFVLIAEKGRLFQQQNPAV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCC
>Mature Secondary Structure 
TDSSQAVSAGRLPMGKREFIALAAFLMAVNSLAIDIMLPALQQIGASLGVESENHRQFV
CCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
VSTYLLGFGCAQLFYGPLSDRFGRRTPLLIGLVIYILSAISIVFVPTFAGLLALRFVQGI
HHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSAATRVITVSIVRDIYGGRQMAEVMSLIMMVFMIVPVIAPGTGQIVMFFGNWHLIFLFI
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHH
AAMAIAIAVWAYIRLPETLHPQNVRPFTARSIFGAFKLVLTNRIALCYTIASTFIFGALF
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFINSAQQVYVGIYDLGVYFPFAFAGVAIFMSLSSFFNSRFVGKLGMRRLSHGSLIGFIT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
INTIWLIVQMASSEPMPFALFISFFGLAMFQFGWIGSNFNSLAMEPLGHVAGTASSVLGF
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
MSTVGGALIGAGIGQAFDGTALPMVAGYFIVSIIGLIFVLIAEKGRLFQQQNPAV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]