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The map label for this gene is queA

Identifier: 209549347

GI number: 209549347

Start: 1791939

End: 1793078

Strand: Direct

Name: queA

Synonym: Rleg2_1751

Alternate gene names: 209549347

Gene position: 1791939-1793078 (Clockwise)

Preceding gene: 209549346

Following gene: 209549348

Centisome position: 39.49

GC content: 64.65

Gene sequence:

>1140_bases
ATGCGCGTAGACCTTTTCGATTTCGATCTGCCGGATGAACGCATCGCGTTGCGGCCTGCCGAGCCGCGCGACAGCGCGCG
CCTGCTCGTCGTCAATCCGCATGCGGAAAGCAGGCCCCATGCGGAAAGCGTACCCCATGCGGAAAGCAGGCCCCATGCGG
AAAGCGCGTTCTCCGATCACCGCGTCGGGGATCTGCCATCCTTCCTGCGGGCGGGCGATGCGCTGGTCTTCAACGACACA
AAGGTCATTCCCGCTCAGTTGGAAGGCATTCGCCATCGCGACGGCGCCGGCGGCCAGCAGGTGTCAGCAACGCTGCATAT
GCGCGTCGGCCCCAGCCGCTGGAAGGCCTTTGCCAAGCCCGGCAAGCGCATCAAGGAGGGCGACCGCATCGCCTTCGGTC
ATTCGGGTGAAAGCTGCTTGCTCGGTTCGCTCGATGCCACCGTCGAGGAAAAGGGCGAAGCGGGCGAAGTGACGCTTGCC
TTCGATCTTTCCGGCCCGGCGCTCGACGAGGCGATCGCCGCCGTCGGCCACATTCCGCTGCCGCCCTATATCGCCGCCAA
GCGTCCCGAGGACGAGCGCGACCGCGCGGACTATCAGACGATCTATGCCCGCGAGGAGGGCGCCGTCGCCGCGCCCACCG
CCGGCCTGCATTTCACGCCCGCGCTGTTCGAAGCGCTCGACAGAGCCGGCATAGAACGTCACTTCGTCACGCTGCATGTC
GGCGCCGGCACCTTCCTGCCCGTCAAGGCCGACGATACCGCCGATCACAAGATGCATCTGGAAAGCGGCACTGTCAGCGC
AGAGACCGCCGCCCGGCTGAACGCCGTCAAGGCAAGGGGCGGGCGCATCGTCTGCGTCGGCACCACCTCGCTGCGGCTGA
TCGAAAGTGCCGCGGAAGAAAGCGGCGAGATCCGCGGCTGGAGCGGCGCCACCGGCATTTTCATCACGCCCGGCTACCGT
TTCAAGGCTGTGGACATGCTGATGACCAATTTCCACCTGCCGCGCTCGACGCTGTTCATGCTGGTCTCGGCCTTTTCAGG
CTTCGAAACCATGCACGCGGCCTATAAACACGCCATTTCGACAGGCTACCGCTTCTACTCCTACGGCGACGCGAGCCTTC
TTTTCCGGAAAGACAAATGA

Upstream 100 bases:

>100_bases
ATGCGGGTTGCCGCCGACGTCTGATTGTGATTTCTGCTGAAACCCGCTCTTGCGCGTCAACCGCGTGGGGGCGGGTTTCG
CTTTGCCTGAAAGTGCCGTT

Downstream 100 bases:

>100_bases
CAGAGAACTTCCAATTCACGTTGAAGAAGACCGATGCCGGCGCGCGCCTCGGCGAAATCTCCATGCCGCGCGGCACGATC
CGCACGCCGGCCTTCATGCC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRVDLFDFDLPDERIALRPAEPRDSARLLVVNPHAESRPHAESVPHAESRPHAESAFSDHRVGDLPSFLRAGDALVFNDT
KVIPAQLEGIRHRDGAGGQQVSATLHMRVGPSRWKAFAKPGKRIKEGDRIAFGHSGESCLLGSLDATVEEKGEAGEVTLA
FDLSGPALDEAIAAVGHIPLPPYIAAKRPEDERDRADYQTIYAREEGAVAAPTAGLHFTPALFEALDRAGIERHFVTLHV
GAGTFLPVKADDTADHKMHLESGTVSAETAARLNAVKARGGRIVCVGTTSLRLIESAAEESGEIRGWSGATGIFITPGYR
FKAVDMLMTNFHLPRSTLFMLVSAFSGFETMHAAYKHAISTGYRFYSYGDASLLFRKDK

Sequences:

>Translated_379_residues
MRVDLFDFDLPDERIALRPAEPRDSARLLVVNPHAESRPHAESVPHAESRPHAESAFSDHRVGDLPSFLRAGDALVFNDT
KVIPAQLEGIRHRDGAGGQQVSATLHMRVGPSRWKAFAKPGKRIKEGDRIAFGHSGESCLLGSLDATVEEKGEAGEVTLA
FDLSGPALDEAIAAVGHIPLPPYIAAKRPEDERDRADYQTIYAREEGAVAAPTAGLHFTPALFEALDRAGIERHFVTLHV
GAGTFLPVKADDTADHKMHLESGTVSAETAARLNAVKARGGRIVCVGTTSLRLIESAAEESGEIRGWSGATGIFITPGYR
FKAVDMLMTNFHLPRSTLFMLVSAFSGFETMHAAYKHAISTGYRFYSYGDASLLFRKDK
>Mature_379_residues
MRVDLFDFDLPDERIALRPAEPRDSARLLVVNPHAESRPHAESVPHAESRPHAESAFSDHRVGDLPSFLRAGDALVFNDT
KVIPAQLEGIRHRDGAGGQQVSATLHMRVGPSRWKAFAKPGKRIKEGDRIAFGHSGESCLLGSLDATVEEKGEAGEVTLA
FDLSGPALDEAIAAVGHIPLPPYIAAKRPEDERDRADYQTIYAREEGAVAAPTAGLHFTPALFEALDRAGIERHFVTLHV
GAGTFLPVKADDTADHKMHLESGTVSAETAARLNAVKARGGRIVCVGTTSLRLIESAAEESGEIRGWSGATGIFITPGYR
FKAVDMLMTNFHLPRSTLFMLVSAFSGFETMHAAYKHAISTGYRFYSYGDASLLFRKDK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=377, Percent_Identity=41.9098143236074, Blast_Score=248, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_RHILW (B5ZPR8)

Other databases:

- EMBL:   CP001191
- RefSeq:   YP_002281264.1
- ProteinModelPortal:   B5ZPR8
- SMR:   B5ZPR8
- GeneID:   6980488
- GenomeReviews:   CP001191_GR
- KEGG:   rlt:Rleg2_1751
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 41152; Mature: 41152

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLFDFDLPDERIALRPAEPRDSARLLVVNPHAESRPHAESVPHAESRPHAESAFSDH
CEEEEECCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCC
RVGDLPSFLRAGDALVFNDTKVIPAQLEGIRHRDGAGGQQVSATLHMRVGPSRWKAFAKP
CCCCCHHHHHCCCEEEEECCEECHHHHCCCCCCCCCCCCEEEEEEEEEECHHHHHHHHCC
GKRIKEGDRIAFGHSGESCLLGSLDATVEEKGEAGEVTLAFDLSGPALDEAIAAVGHIPL
CCHHCCCCEEEECCCCCCEEEECCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCC
PPYIAAKRPEDERDRADYQTIYAREEGAVAAPTAGLHFTPALFEALDRAGIERHFVTLHV
CCCEECCCCCCHHHHCCCEEEEEECCCCEECCCCCCEECHHHHHHHHHCCCCEEEEEEEE
GAGTFLPVKADDTADHKMHLESGTVSAETAARLNAVKARGGRIVCVGTTSLRLIESAAEE
CCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHC
SGEIRGWSGATGIFITPGYRFKAVDMLMTNFHLPRSTLFMLVSAFSGFETMHAAYKHAIS
CCCEECCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
TGYRFYSYGDASLLFRKDK
CCEEEEEECCCEEEEEECC
>Mature Secondary Structure
MRVDLFDFDLPDERIALRPAEPRDSARLLVVNPHAESRPHAESVPHAESRPHAESAFSDH
CEEEEECCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCC
RVGDLPSFLRAGDALVFNDTKVIPAQLEGIRHRDGAGGQQVSATLHMRVGPSRWKAFAKP
CCCCCHHHHHCCCEEEEECCEECHHHHCCCCCCCCCCCCEEEEEEEEEECHHHHHHHHCC
GKRIKEGDRIAFGHSGESCLLGSLDATVEEKGEAGEVTLAFDLSGPALDEAIAAVGHIPL
CCHHCCCCEEEECCCCCCEEEECCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCC
PPYIAAKRPEDERDRADYQTIYAREEGAVAAPTAGLHFTPALFEALDRAGIERHFVTLHV
CCCEECCCCCCHHHHCCCEEEEEECCCCEECCCCCCEECHHHHHHHHHCCCCEEEEEEEE
GAGTFLPVKADDTADHKMHLESGTVSAETAARLNAVKARGGRIVCVGTTSLRLIESAAEE
CCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHC
SGEIRGWSGATGIFITPGYRFKAVDMLMTNFHLPRSTLFMLVSAFSGFETMHAAYKHAIS
CCCEECCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
TGYRFYSYGDASLLFRKDK
CCEEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA