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The map label for this gene is purU [H]

Identifier: 209549227

GI number: 209549227

Start: 1656737

End: 1657633

Strand: Direct

Name: purU [H]

Synonym: Rleg2_1628

Alternate gene names: 209549227

Gene position: 1656737-1657633 (Clockwise)

Preceding gene: 209549226

Following gene: 209549228

Centisome position: 36.51

GC content: 59.53

Gene sequence:

>897_bases
ATGACCACCACGGCTCCTCGCTACGCTCTTCGTGTGGCATGCCCGTCAATCCGCGGCGTGACGGCCGCCATCGCCACCTA
TCTGTCGCAGAGCGGTTGCAACATCTCGGACAGTGCGCAATTCGATGACGCGGACACCGGAAGATATTTCATGCGGATCA
GCTTCCAGCCGCAGGACGGACATACGCTTGGGCAGCTTCGTGACGGCTTCGAGCCGATCGCCGACAGGTTCGAGGCGAAC
GCCGAATTCTTCGACGAAGCCGAAAAGAAGAAGGTGATCCTCATGGTCAGCCGCTTCGGACACTGCCTGAACGATCTTCT
CTACCGCTGGCGGATCGGGGCGCTTCCGATCGACATCGTCGGCGTGATCTCCAACCACATGGATTACCAGCGGATCGTCG
TGAACCACGACATCCCGTTCCACTGCATCAAGGTCACTAGGGAAAATAAGCCGGAGGCCGAGGCGAAACAGATGCAGATC
GTCGAGGGCTCGGGAGCCGAACTCGTCGTACTGGCTCGATATATGCAGGTCCTGTCCGACGAAATGTGCCGCAAGATGTC
CGGCAGGATCATCAATATTCACCACTCGTTTCTGCCGAGCTTCAAGGGCGCCAATCCTTACAAGCAGGCGTTCGAACGGG
GCGTGAAGCTCATCGGCGCGACGTCGCACTACGTGACGGCAGACCTCGACGAAGGTCCGATCATCGAGCAGGATATCGTC
CGCGTCACGCATGCGCAGAGCGGCGAGGACTATGTGAGCCTCGGCCGCGACGTCGAAAGCCAGGTACTTGCCCGAGCCAT
CCACGCCCACATCCATGGCCGTGTGTTCATCAACGGCAACAAGACAGTTGTATTCCCCGCTTCACCGGGCTCCTACGCAT
CGGAGCGCATGGGCTGA

Upstream 100 bases:

>100_bases
CGATGACGATCGCATGCCTGCTGTCCAACACGCTCACGGCATTCCGCCGCCACCACGCCATGGTCGCCACGGCCTGAGAA
GAAGAACAAGGATAGTAGAT

Downstream 100 bases:

>100_bases
CCGCGGCATAGCCTATCCTCAAATCAGCGGGAGCGGGATCACCCATCGAACTCCCCGCGTCGGCTGTGGGCAACTCTCCT
TACTGAGGGACCACGTTCAT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTTTAPRYALRVACPSIRGVTAAIATYLSQSGCNISDSAQFDDADTGRYFMRISFQPQDGHTLGQLRDGFEPIADRFEAN
AEFFDEAEKKKVILMVSRFGHCLNDLLYRWRIGALPIDIVGVISNHMDYQRIVVNHDIPFHCIKVTRENKPEAEAKQMQI
VEGSGAELVVLARYMQVLSDEMCRKMSGRIINIHHSFLPSFKGANPYKQAFERGVKLIGATSHYVTADLDEGPIIEQDIV
RVTHAQSGEDYVSLGRDVESQVLARAIHAHIHGRVFINGNKTVVFPASPGSYASERMG

Sequences:

>Translated_298_residues
MTTTAPRYALRVACPSIRGVTAAIATYLSQSGCNISDSAQFDDADTGRYFMRISFQPQDGHTLGQLRDGFEPIADRFEAN
AEFFDEAEKKKVILMVSRFGHCLNDLLYRWRIGALPIDIVGVISNHMDYQRIVVNHDIPFHCIKVTRENKPEAEAKQMQI
VEGSGAELVVLARYMQVLSDEMCRKMSGRIINIHHSFLPSFKGANPYKQAFERGVKLIGATSHYVTADLDEGPIIEQDIV
RVTHAQSGEDYVSLGRDVESQVLARAIHAHIHGRVFINGNKTVVFPASPGSYASERMG
>Mature_297_residues
TTTAPRYALRVACPSIRGVTAAIATYLSQSGCNISDSAQFDDADTGRYFMRISFQPQDGHTLGQLRDGFEPIADRFEANA
EFFDEAEKKKVILMVSRFGHCLNDLLYRWRIGALPIDIVGVISNHMDYQRIVVNHDIPFHCIKVTRENKPEAEAKQMQIV
EGSGAELVVLARYMQVLSDEMCRKMSGRIINIHHSFLPSFKGANPYKQAFERGVKLIGATSHYVTADLDEGPIIEQDIVR
VTHAQSGEDYVSLGRDVESQVLARAIHAHIHGRVFINGNKTVVFPASPGSYASERMG

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=217, Percent_Identity=29.4930875576037, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI209869995, Length=217, Percent_Identity=29.4930875576037, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI209869993, Length=217, Percent_Identity=29.4930875576037, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1787483, Length=281, Percent_Identity=39.8576512455516, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1788846, Length=114, Percent_Identity=34.2105263157895, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=163, Percent_Identity=31.2883435582822, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24582400, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33216; Mature: 33085

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTAPRYALRVACPSIRGVTAAIATYLSQSGCNISDSAQFDDADTGRYFMRISFQPQDG
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
HTLGQLRDGFEPIADRFEANAEFFDEAEKKKVILMVSRFGHCLNDLLYRWRIGALPIDIV
CHHHHHHCCHHHHHHHHCCCHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHH
GVISNHMDYQRIVVNHDIPFHCIKVTRENKPEAEAKQMQIVEGSGAELVVLARYMQVLSD
HHHHCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHH
EMCRKMSGRIINIHHSFLPSFKGANPYKQAFERGVKLIGATSHYVTADLDEGPIIEQDIV
HHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHHCCEEEECCCCEEEEECCCCCCCHHHHH
RVTHAQSGEDYVSLGRDVESQVLARAIHAHIHGRVFINGNKTVVFPASPGSYASERMG
HHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCEEEEECCCEEEEEECCCCCCHHHCCC
>Mature Secondary Structure 
TTTAPRYALRVACPSIRGVTAAIATYLSQSGCNISDSAQFDDADTGRYFMRISFQPQDG
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
HTLGQLRDGFEPIADRFEANAEFFDEAEKKKVILMVSRFGHCLNDLLYRWRIGALPIDIV
CHHHHHHCCHHHHHHHHCCCHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHH
GVISNHMDYQRIVVNHDIPFHCIKVTRENKPEAEAKQMQIVEGSGAELVVLARYMQVLSD
HHHHCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHH
EMCRKMSGRIINIHHSFLPSFKGANPYKQAFERGVKLIGATSHYVTADLDEGPIIEQDIV
HHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHHCCEEEECCCCEEEEECCCCCCCHHHHH
RVTHAQSGEDYVSLGRDVESQVLARAIHAHIHGRVFINGNKTVVFPASPGSYASERMG
HHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCEEEEECCCEEEEEECCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]