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The map label for this gene is folD2 [H]

Identifier: 209549226

GI number: 209549226

Start: 1655817

End: 1656713

Strand: Direct

Name: folD2 [H]

Synonym: Rleg2_1627

Alternate gene names: 209549226

Gene position: 1655817-1656713 (Clockwise)

Preceding gene: 209549225

Following gene: 209549227

Centisome position: 36.49

GC content: 60.42

Gene sequence:

>897_bases
ATGGGAATGGATAAATTCACACGCGCGGCGAACATCGACGGCAAAGAATATGCCGCAGAGCTCCTCGAGCGCATCAGGGC
TGACGTCGAAACGCTGAAGGCAACGACAGGCGCCGTTCCCGGTCTGGCCGTCGTTCTGGTGGGCGACGATCCGGCAAGCG
CAATTTACGTGGCTTCAAAGCAGCGTCAGACGGTCGCTGCAGGTATGAAGTCCTTTGAGTATCGGCTCGCGGCGGAAACC
TCGCAGGACGAATTGCTCGAGCTCGTGCATCGCCTTAATGAGGATCCGGAAGTATATGGAATTCTTGTACAGTTGCCGCT
GCCGGTTCATCTCGATCCAAATGCCGTGATCCAGGCCATCAATCCCGACAAGGATGTCGATGGCTTCCATGTGTCGAATG
CCGGCCGCCTTGCCACGGGCAACCCGGCCCTGGTTCCTTGCACTCCTCTAGGCTGCATGATGCTTATCAAGGCGACGCTC
GGCGAAAACCTCTCCGGCCTTCATGCCGTCATCGTCGGCAAGTCCAATATCGTTGGAAAGCCAATGGCGCAGATGCTGAT
GAACGCACACTGTACCATCACGGTTGTGCATTCGCAGACAAGGAACGCGGCGGAGCTTTGCCGGGCGGCCGACATTCTTG
TCGTCGCGGCCGGTAGAGCGGGTCTCGTTCGCGGCGATTGGATCAAGCCTGGCGCCGTCGTGATCGACGTCGGTATCAAC
CGGATCCAGGACGGCGAAAAATCCCGGATCGTCGGCGATGTGGCATATGGCGAAGCCGGCCACGCACGCGCCATCACGCC
CGTGCCCGGCGGCGTCGGTCCGATGACGATCGCATGCCTGCTGTCCAACACGCTCACGGCATTCCGCCGCCACCACGCCA
TGGTCGCCACGGCCTGA

Upstream 100 bases:

>100_bases
GTTCCCGGTCTACGGTCACTGAGAATGCGGAAATGCCGTGCCGACATCTTAAAGGCGCGGCACAGCTTGCTGGTCGAGCT
TGGATGCAATGGAGCACCCC

Downstream 100 bases:

>100_bases
GAAGAAGAACAAGGATAGTAGATATGACCACCACGGCTCCTCGCTACGCTCTTCGTGTGGCATGCCCGTCAATCCGCGGC
GTGACGGCCGCCATCGCCAC

Product: methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MGMDKFTRAANIDGKEYAAELLERIRADVETLKATTGAVPGLAVVLVGDDPASAIYVASKQRQTVAAGMKSFEYRLAAET
SQDELLELVHRLNEDPEVYGILVQLPLPVHLDPNAVIQAINPDKDVDGFHVSNAGRLATGNPALVPCTPLGCMMLIKATL
GENLSGLHAVIVGKSNIVGKPMAQMLMNAHCTITVVHSQTRNAAELCRAADILVVAAGRAGLVRGDWIKPGAVVIDVGIN
RIQDGEKSRIVGDVAYGEAGHARAITPVPGGVGPMTIACLLSNTLTAFRRHHAMVATA

Sequences:

>Translated_298_residues
MGMDKFTRAANIDGKEYAAELLERIRADVETLKATTGAVPGLAVVLVGDDPASAIYVASKQRQTVAAGMKSFEYRLAAET
SQDELLELVHRLNEDPEVYGILVQLPLPVHLDPNAVIQAINPDKDVDGFHVSNAGRLATGNPALVPCTPLGCMMLIKATL
GENLSGLHAVIVGKSNIVGKPMAQMLMNAHCTITVVHSQTRNAAELCRAADILVVAAGRAGLVRGDWIKPGAVVIDVGIN
RIQDGEKSRIVGDVAYGEAGHARAITPVPGGVGPMTIACLLSNTLTAFRRHHAMVATA
>Mature_297_residues
GMDKFTRAANIDGKEYAAELLERIRADVETLKATTGAVPGLAVVLVGDDPASAIYVASKQRQTVAAGMKSFEYRLAAETS
QDELLELVHRLNEDPEVYGILVQLPLPVHLDPNAVIQAINPDKDVDGFHVSNAGRLATGNPALVPCTPLGCMMLIKATLG
ENLSGLHAVIVGKSNIVGKPMAQMLMNAHCTITVVHSQTRNAAELCRAADILVVAAGRAGLVRGDWIKPGAVVIDVGINR
IQDGEKSRIVGDVAYGEAGHARAITPVPGGVGPMTIACLLSNTLTAFRRHHAMVATA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=44.7916666666667, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=42.320819112628, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI94721354, Length=292, Percent_Identity=42.4657534246575, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI36796743, Length=199, Percent_Identity=25.6281407035176, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1786741, Length=282, Percent_Identity=50, Blast_Score=256, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI17568735, Length=287, Percent_Identity=40.7665505226481, Blast_Score=198, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=42.8571428571429, Blast_Score=229, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=40.8783783783784, Blast_Score=216, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6322933, Length=187, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI62472483, Length=305, Percent_Identity=43.9344262295082, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI45551871, Length=305, Percent_Identity=43.9344262295082, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24645718, Length=298, Percent_Identity=44.2953020134228, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI17137370, Length=298, Percent_Identity=44.2953020134228, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI17136816, Length=305, Percent_Identity=45.2459016393443, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=45.6081081081081, Blast_Score=226, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31403; Mature: 31272

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMDKFTRAANIDGKEYAAELLERIRADVETLKATTGAVPGLAVVLVGDDPASAIYVASK
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECC
QRQTVAAGMKSFEYRLAAETSQDELLELVHRLNEDPEVYGILVQLPLPVHLDPNAVIQAI
CHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCEEECHHHEEEEC
NPDKDVDGFHVSNAGRLATGNPALVPCTPLGCMMLIKATLGENLSGLHAVIVGKSNIVGK
CCCCCCCCEEECCCCCEECCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
PMAQMLMNAHCTITVVHSQTRNAAELCRAADILVVAAGRAGLVRGDWIKPGAVVIDVGIN
HHHHHHHCCCEEEEEEECCCCCHHHHHHHCCEEEEECCCCCCEECCCCCCCCEEEECCCC
RIQDGEKSRIVGDVAYGEAGHARAITPVPGGVGPMTIACLLSNTLTAFRRHHAMVATA
CCCCCCCCEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCEEEECC
>Mature Secondary Structure 
GMDKFTRAANIDGKEYAAELLERIRADVETLKATTGAVPGLAVVLVGDDPASAIYVASK
CCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECC
QRQTVAAGMKSFEYRLAAETSQDELLELVHRLNEDPEVYGILVQLPLPVHLDPNAVIQAI
CHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCEEECHHHEEEEC
NPDKDVDGFHVSNAGRLATGNPALVPCTPLGCMMLIKATLGENLSGLHAVIVGKSNIVGK
CCCCCCCCEEECCCCCEECCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
PMAQMLMNAHCTITVVHSQTRNAAELCRAADILVVAAGRAGLVRGDWIKPGAVVIDVGIN
HHHHHHHCCCEEEEEEECCCCCHHHHHHHCCEEEEECCCCCCEECCCCCCCCEEEECCCC
RIQDGEKSRIVGDVAYGEAGHARAITPVPGGVGPMTIACLLSNTLTAFRRHHAMVATA
CCCCCCCCEEEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA