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The map label for this gene is glyA [H]

Identifier: 209549225

GI number: 209549225

Start: 1654461

End: 1655738

Strand: Direct

Name: glyA [H]

Synonym: Rleg2_1626

Alternate gene names: 209549225

Gene position: 1654461-1655738 (Clockwise)

Preceding gene: 209549219

Following gene: 209549226

Centisome position: 36.46

GC content: 61.97

Gene sequence:

>1278_bases
ATGACCAACGCTGCAAGGGTGCATTTCAACACCACCGTCGAAAAGGCTGATCCACTTGTCGCCGATGCTCTCGCCGCCGA
GCGGGCCCGACAGCAGAACCAGATTGAACTCATCGCCTCGGAGAATATCGTCAGCCGCGCGGTTCTGGACGCCTTGGGGC
ACGAGATCACCAACAAGACGCTCGAAGGTTATCCGGGCAACCGTTTTCACGGTGGCGGACAGTTTGTCGACATTGCAGAG
CAGGCCGCGATCGACCGGGCGAAGCAGCTCTTCAGCTGCGGTTATGCCAACGTCCAGCCGCACTCGGGGACACAGGCCAA
CCTTGCCGTCTTCTTCCTGCTTCTGAAGCCGGGCGAAAAGGTCCTGTCGCTGGACCTCGCGGCTGGCGGCCACCTCTCGC
ACGGCATGAAGGCGAACCTGTCGGGCCGCTGGTTCGACGCCGCCAATTACAACGTCAATCCGCAGAACGAGGTCATCGAC
CTTGATGAGATGGAACGCATCGCCGAAGAGATCCGCCCGAAGCTGCTGATCACCGGTGGCTCCGCCTATCCGCGCGAACT
CGACTTCGAGCGCATGTCGGGGATCGCCAAGAAGGTCGGCGCCTACTTCCTGGTGGATATGGCCCACATCGCCGGCCTCG
TTGCAGGCGGCGTCCATCCTTCGCCATTCCCGCATGCCGACATTGTGACCTGCACCACTACCAAGACGCTGCGCGGTCCG
CGTGGCGGCCTGATCCTCACCAACAATGAGGAATGGTATAAGAAGCTGCAAGCCGCCGTGTTTCCCGGCGTCCAGGGGTC
GCTGCACAGCAACGTCCTGGCTGCGAAGGCGATCTGCTTGGGCGAAGCTCTCCGCGACGACTTCAAAGTCTACGCTCGTC
AGGTCGTAGCCAACGCGAGGGTGCTTGCCGAAACGCTTGCCGATCGTGGCGTTCGTATCGTTTCTGGCGGCACCGACACC
CACATCGTTCTGCTCGATCTATCGAGCAAAGGGTTGATTGGAAAGCAGGCCGAGGCGCTGCTCGCCAAGGCGAACATCAC
CTCCAACAAGAACCCGATCCCCGGCGACAGTCCCCGTCCTCCGGAGTGGGTCGGCATGCGGCTCGGTTCGTCCGCAGCGA
CGACACGCGGCCTGAAGGAAGAGGAATTCCGGGTTCTCGGCCACGTCATCGCGGATCTTATCGACGCCGAAACGAAGGGC
GAGGCGGACGAGATCGTCGGCGCGGCCAAGGCGAAGATCGCGGAACTGACCGAGAAGTTCCCGGTCTACGGTCACTGA

Upstream 100 bases:

>100_bases
ATCATTAGCGCGTCTGAACGTATAAGCAAATAATATTAACTTTTTCTGGTGTGTTGCTCGATCTAGGTTCCTCACAAATT
TCGAGATTTGGAGAACTCAG

Downstream 100 bases:

>100_bases
GAATGCGGAAATGCCGTGCCGACATCTTAAAGGCGCGGCACAGCTTGCTGGTCGAGCTTGGATGCAATGGAGCACCCCAT
GGGAATGGATAAATTCACAC

Product: glycine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTNAARVHFNTTVEKADPLVADALAAERARQQNQIELIASENIVSRAVLDALGHEITNKTLEGYPGNRFHGGGQFVDIAE
QAAIDRAKQLFSCGYANVQPHSGTQANLAVFFLLLKPGEKVLSLDLAAGGHLSHGMKANLSGRWFDAANYNVNPQNEVID
LDEMERIAEEIRPKLLITGGSAYPRELDFERMSGIAKKVGAYFLVDMAHIAGLVAGGVHPSPFPHADIVTCTTTKTLRGP
RGGLILTNNEEWYKKLQAAVFPGVQGSLHSNVLAAKAICLGEALRDDFKVYARQVVANARVLAETLADRGVRIVSGGTDT
HIVLLDLSSKGLIGKQAEALLAKANITSNKNPIPGDSPRPPEWVGMRLGSSAATTRGLKEEEFRVLGHVIADLIDAETKG
EADEIVGAAKAKIAELTEKFPVYGH

Sequences:

>Translated_425_residues
MTNAARVHFNTTVEKADPLVADALAAERARQQNQIELIASENIVSRAVLDALGHEITNKTLEGYPGNRFHGGGQFVDIAE
QAAIDRAKQLFSCGYANVQPHSGTQANLAVFFLLLKPGEKVLSLDLAAGGHLSHGMKANLSGRWFDAANYNVNPQNEVID
LDEMERIAEEIRPKLLITGGSAYPRELDFERMSGIAKKVGAYFLVDMAHIAGLVAGGVHPSPFPHADIVTCTTTKTLRGP
RGGLILTNNEEWYKKLQAAVFPGVQGSLHSNVLAAKAICLGEALRDDFKVYARQVVANARVLAETLADRGVRIVSGGTDT
HIVLLDLSSKGLIGKQAEALLAKANITSNKNPIPGDSPRPPEWVGMRLGSSAATTRGLKEEEFRVLGHVIADLIDAETKG
EADEIVGAAKAKIAELTEKFPVYGH
>Mature_424_residues
TNAARVHFNTTVEKADPLVADALAAERARQQNQIELIASENIVSRAVLDALGHEITNKTLEGYPGNRFHGGGQFVDIAEQ
AAIDRAKQLFSCGYANVQPHSGTQANLAVFFLLLKPGEKVLSLDLAAGGHLSHGMKANLSGRWFDAANYNVNPQNEVIDL
DEMERIAEEIRPKLLITGGSAYPRELDFERMSGIAKKVGAYFLVDMAHIAGLVAGGVHPSPFPHADIVTCTTTKTLRGPR
GGLILTNNEEWYKKLQAAVFPGVQGSLHSNVLAAKAICLGEALRDDFKVYARQVVANARVLAETLADRGVRIVSGGTDTH
IVLLDLSSKGLIGKQAEALLAKANITSNKNPIPGDSPRPPEWVGMRLGSSAATTRGLKEEEFRVLGHVIADLIDAETKGE
ADEIVGAAKAKIAELTEKFPVYGH

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=431, Percent_Identity=43.1554524361949, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI261862350, Length=431, Percent_Identity=43.1554524361949, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI261862348, Length=431, Percent_Identity=43.1554524361949, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI19923315, Length=431, Percent_Identity=43.1554524361949, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI22547186, Length=433, Percent_Identity=42.4942263279446, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI261862346, Length=431, Percent_Identity=42.4593967517401, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI22547189, Length=419, Percent_Identity=42.0047732696897, Blast_Score=291, Evalue=6e-79,
Organism=Escherichia coli, GI1788902, Length=409, Percent_Identity=48.6552567237164, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25144732, Length=415, Percent_Identity=43.1325301204819, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI25144729, Length=415, Percent_Identity=43.1325301204819, Blast_Score=325, Evalue=3e-89,
Organism=Saccharomyces cerevisiae, GI6319739, Length=423, Percent_Identity=40.8983451536643, Blast_Score=319, Evalue=6e-88,
Organism=Saccharomyces cerevisiae, GI6323087, Length=398, Percent_Identity=44.2211055276382, Blast_Score=308, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24640005, Length=463, Percent_Identity=38.8768898488121, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI221329721, Length=463, Percent_Identity=38.8768898488121, Blast_Score=319, Evalue=3e-87,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45631; Mature: 45499

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAARVHFNTTVEKADPLVADALAAERARQQNQIELIASENIVSRAVLDALGHEITNKT
CCCCEEEEEECCHHHCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCH
LEGYPGNRFHGGGQFVDIAEQAAIDRAKQLFSCGYANVQPHSGTQANLAVFFLLLKPGEK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCE
VLSLDLAAGGHLSHGMKANLSGRWFDAANYNVNPQNEVIDLDEMERIAEEIRPKLLITGG
EEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEECHHHHHHHHHHCCCEEEEECC
SAYPRELDFERMSGIAKKVGAYFLVDMAHIAGLVAGGVHPSPFPHADIVTCTTTKTLRGP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHCCCC
RGGLILTNNEEWYKKLQAAVFPGVQGSLHSNVLAAKAICLGEALRDDFKVYARQVVANAR
CCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAETLADRGVRIVSGGTDTHIVLLDLSSKGLIGKQAEALLAKANITSNKNPIPGDSPRP
HHHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
PEWVGMRLGSSAATTRGLKEEEFRVLGHVIADLIDAETKGEADEIVGAAKAKIAELTEKF
CHHHHEECCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
PVYGH
CCCCC
>Mature Secondary Structure 
TNAARVHFNTTVEKADPLVADALAAERARQQNQIELIASENIVSRAVLDALGHEITNKT
CCCEEEEEECCHHHCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCH
LEGYPGNRFHGGGQFVDIAEQAAIDRAKQLFSCGYANVQPHSGTQANLAVFFLLLKPGEK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCE
VLSLDLAAGGHLSHGMKANLSGRWFDAANYNVNPQNEVIDLDEMERIAEEIRPKLLITGG
EEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEECHHHHHHHHHHCCCEEEEECC
SAYPRELDFERMSGIAKKVGAYFLVDMAHIAGLVAGGVHPSPFPHADIVTCTTTKTLRGP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHCCCC
RGGLILTNNEEWYKKLQAAVFPGVQGSLHSNVLAAKAICLGEALRDDFKVYARQVVANAR
CCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAETLADRGVRIVSGGTDTHIVLLDLSSKGLIGKQAEALLAKANITSNKNPIPGDSPRP
HHHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
PEWVGMRLGSSAATTRGLKEEEFRVLGHVIADLIDAETKGEADEIVGAAKAKIAELTEKF
CHHHHEECCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
PVYGH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA