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The map label for this gene is dus [H]

Identifier: 209549216

GI number: 209549216

Start: 1642341

End: 1643288

Strand: Direct

Name: dus [H]

Synonym: Rleg2_1617

Alternate gene names: 209549216

Gene position: 1642341-1643288 (Clockwise)

Preceding gene: 209549213

Following gene: 209549217

Centisome position: 36.19

GC content: 66.56

Gene sequence:

>948_bases
GTGCGGAACCGTGTTGTGCTGGCGCCGATGTCCGGCGTCACGGACATGCCCTTCCGCGAGCTCGCCTGGCGCTTCGGCGC
CGGCCTCGTCGTCACCGAAATGGTGGCGAGCCGCGAACTGGTCAACGACACGGCCGAATCCTGGTCGCGGCTGAGAGCAG
CGGGCTTCCGGCCGCACATGGTGCAGCTCGCCGGCCGGGAGGCGCACTGGATGGCGGAGGCGGCGAAGATCGCTGCCGAT
CACGGCGCCGATATCATCGACATCAATATGGGCTGCCCGGCAAAGAAGGTGATCGGCGGTTATTCCGGCTCGGCGCTGAT
GCGCGATCCCGATCACGCGCTCGGCCTCATCGAGGCGACGGTCAAGGCCGTCGATATTCCCGTGACGCTGAAAATGCGCC
TCGGCTGGGATGAGAACTCGATCAACGCGCCCGAGATCGCGCGCCGCGCCGAGGCGGCCGGCATTCAGCTCGTGACCATT
CATGGGCGCACCCGCATGCAATTCTATGAAGGCCGCGCCGATTGGGATGCGATCCGTCCCGTCCGCGACGTCATCTCCAT
CCCGCTGATCGCCAATGGCGATGTCGAGACCGCCGAAGATGCGCAGGAAATCCTGCGCCGCTCCGGCGCCGATGCCGTCA
TGATCGGCCGGGGCTGCCAGGGCAGGCCGTGGCATGCCGGTGTGCTCGCCGGTGGAGCGGAGCCGCGACGCGAAGACATT
GCCGATATTGCCGTCGAACATTATCGGATGATGCTCGATTTCTACGGCGAGGCGGTGGCGATCCGCCACGCCCGCAAGCA
CCTCGGCTGGTATCTCGAGCGTTTCGCCGCTGATCTTGCCGGCGGCGACAAGGCTGCGATCATGACCTCGCGCGATCCGC
GCGAGGTGGCCGCGCGCCTTTACGAGGCTTTGGCATCGGGTGTTGTCGACAGCCGGGAGGCGGCATGA

Upstream 100 bases:

>100_bases
GAACAATGGCTAAAAATCATGCACTTCCACAGTGTGCCCGAAAGATAATCATTTGATTTCCAAGGACCTCGCAGCGCCTT
TCCGAATAGGACCCGTATCG

Downstream 100 bases:

>100_bases
CGAAGGATACGACTTCTCCGCCCGATCAGGCCGGCGGAACCGTCGCCATGGCCGTGCTCAACGCCATCCAGAACCCCGTC
GTCATGGTCGACGAATCCGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRNRVVLAPMSGVTDMPFRELAWRFGAGLVVTEMVASRELVNDTAESWSRLRAAGFRPHMVQLAGREAHWMAEAAKIAAD
HGADIIDINMGCPAKKVIGGYSGSALMRDPDHALGLIEATVKAVDIPVTLKMRLGWDENSINAPEIARRAEAAGIQLVTI
HGRTRMQFYEGRADWDAIRPVRDVISIPLIANGDVETAEDAQEILRRSGADAVMIGRGCQGRPWHAGVLAGGAEPRREDI
ADIAVEHYRMMLDFYGEAVAIRHARKHLGWYLERFAADLAGGDKAAIMTSRDPREVAARLYEALASGVVDSREAA

Sequences:

>Translated_315_residues
MRNRVVLAPMSGVTDMPFRELAWRFGAGLVVTEMVASRELVNDTAESWSRLRAAGFRPHMVQLAGREAHWMAEAAKIAAD
HGADIIDINMGCPAKKVIGGYSGSALMRDPDHALGLIEATVKAVDIPVTLKMRLGWDENSINAPEIARRAEAAGIQLVTI
HGRTRMQFYEGRADWDAIRPVRDVISIPLIANGDVETAEDAQEILRRSGADAVMIGRGCQGRPWHAGVLAGGAEPRREDI
ADIAVEHYRMMLDFYGEAVAIRHARKHLGWYLERFAADLAGGDKAAIMTSRDPREVAARLYEALASGVVDSREAA
>Mature_315_residues
MRNRVVLAPMSGVTDMPFRELAWRFGAGLVVTEMVASRELVNDTAESWSRLRAAGFRPHMVQLAGREAHWMAEAAKIAAD
HGADIIDINMGCPAKKVIGGYSGSALMRDPDHALGLIEATVKAVDIPVTLKMRLGWDENSINAPEIARRAEAAGIQLVTI
HGRTRMQFYEGRADWDAIRPVRDVISIPLIANGDVETAEDAQEILRRSGADAVMIGRGCQGRPWHAGVLAGGAEPRREDI
ADIAVEHYRMMLDFYGEAVAIRHARKHLGWYLERFAADLAGGDKAAIMTSRDPREVAARLYEALASGVVDSREAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=261, Percent_Identity=31.0344827586207, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI239788462, Length=261, Percent_Identity=31.0344827586207, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI31742496, Length=279, Percent_Identity=27.9569892473118, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI40807366, Length=259, Percent_Identity=32.046332046332, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI8923374, Length=258, Percent_Identity=28.2945736434109, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1789660, Length=288, Percent_Identity=45.4861111111111, Blast_Score=238, Evalue=3e-64,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=38.0952380952381, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI145693211, Length=246, Percent_Identity=26.8292682926829, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=260, Percent_Identity=32.6923076923077, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25144369, Length=268, Percent_Identity=29.4776119402985, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17507177, Length=223, Percent_Identity=31.390134529148, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=241, Percent_Identity=28.6307053941909, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323560, Length=273, Percent_Identity=31.1355311355311, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323433, Length=252, Percent_Identity=28.968253968254, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=28.7610619469027, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24580595, Length=237, Percent_Identity=35.0210970464135, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19920448, Length=237, Percent_Identity=35.0210970464135, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24585320, Length=235, Percent_Identity=32.7659574468085, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI45549423, Length=241, Percent_Identity=31.5352697095436, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19921524, Length=223, Percent_Identity=30.4932735426009, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34430; Mature: 34430

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNRVVLAPMSGVTDMPFRELAWRFGAGLVVTEMVASRELVNDTAESWSRLRAAGFRPHM
CCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VQLAGREAHWMAEAAKIAADHGADIIDINMGCPAKKVIGGYSGSALMRDPDHALGLIEAT
HHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHH
VKAVDIPVTLKMRLGWDENSINAPEIARRAEAAGIQLVTIHGRTRMQFYEGRADWDAIRP
HHHEECCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEEECCEEEEEECCCCCHHHHHH
VRDVISIPLIANGDVETAEDAQEILRRSGADAVMIGRGCQGRPWHAGVLAGGAEPRREDI
HHHHHHCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCHHHHH
ADIAVEHYRMMLDFYGEAVAIRHARKHLGWYLERFAADLAGGDKAAIMTSRDPREVAARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
YEALASGVVDSREAA
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MRNRVVLAPMSGVTDMPFRELAWRFGAGLVVTEMVASRELVNDTAESWSRLRAAGFRPHM
CCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VQLAGREAHWMAEAAKIAADHGADIIDINMGCPAKKVIGGYSGSALMRDPDHALGLIEAT
HHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHH
VKAVDIPVTLKMRLGWDENSINAPEIARRAEAAGIQLVTIHGRTRMQFYEGRADWDAIRP
HHHEECCEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEEECCEEEEEECCCCCHHHHHH
VRDVISIPLIANGDVETAEDAQEILRRSGADAVMIGRGCQGRPWHAGVLAGGAEPRREDI
HHHHHHCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCHHHHH
ADIAVEHYRMMLDFYGEAVAIRHARKHLGWYLERFAADLAGGDKAAIMTSRDPREVAARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
YEALASGVVDSREAA
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]