Click here to switch to the map view.

The map label for this gene is ntrB [H]

Identifier: 209549217

GI number: 209549217

Start: 1643285

End: 1644439

Strand: Direct

Name: ntrB [H]

Synonym: Rleg2_1618

Alternate gene names: 209549217

Gene position: 1643285-1644439 (Clockwise)

Preceding gene: 209549216

Following gene: 209549218

Centisome position: 36.21

GC content: 64.33

Gene sequence:

>1155_bases
ATGACGAAGGATACGACTTCTCCGCCCGATCAGGCCGGCGGAACCGTCGCCATGGCCGTGCTCAACGCCATCCAGAACCC
CGTCGTCATGGTCGACGAATCCGGCTTCGTCGTTTTCGCCAATTGGGAGGCGGAAGCCTTCTTCGGCGCCAGCGCCCCGC
ATCTGGCGCGTTATCGCATCTCGACCTTCATTCCCTTCGGTAGCCCGCTGCTCGCCCTGATCGACCAGGTGCGGGAGCGC
AAGGCGCCGGTCAACGAATATCGCGTCGACCTGAGTTCGCCCCGCCTCGGCCAGGACAAGCTCGTCGATCTCTACGTCGC
CCCGGTGCTCAGCGAGCCCGGCGCCGTCGTCATCGTCTTTCAGGAACGCTCGATGGCCGACAAGATCGACCGGCAGCTGA
CGCATCGCGCCGCCGCCCGCTCGGTGACCGGTCTCGCCTCGATGCTGGCGCACGAAATCAAGAACCCGCTCTCCGGCATC
CGCGGCGCTGCCCAGCTGCTTGAACAATCCGCGGTCGACGACGACCGGGCGCTGACCCGGCTGATCTGCGACGAAACCGA
CCGGATCGTCTCGCTGGTCGATCGCATGGAAGTTTTTTCCGACGAACGTCCGGTCGACCGCATGCCGGTCAACATCCATT
CCGTGCTCGATCATGTGAAGGCCGTCGCCAGGGCGGGTTTTGCCCGCAACATCCGCGTGACCGAGAGTTACGACCCGTCG
CTGCCGGCCGTCTATGCCAATCGCGACCAGCTCGTGCAGGTCTTCCTCAATCTGGTGAAAAATGCTGCCGAAGCGGTCGG
CGATCGGCCGGATGGCGAGATCCTGCTGACGACGGCCTATCGTCCCGGCATCCGTCTCTCGGTCGCCGGCACGCGCGAGA
AAATCTCGCTGCCGCTCGAATTCTGCGTCCACGACAATGGCCCCGGCGTTCCCGCCGATCTGCTGCCGCATCTTTTCGAT
CCGTTCATCACCACCAAGACCAACGGCAGCGGCCTCGGCCTGGCGCTGGTCGCCAAGATCATCGGCGATCACGGCGGCAT
CATCGAATGCGACAGCCAGAACAGCCGCACGACATTCCGCGTCCTCATGCCGGCGTCGAAGGACGCCTCGCTCGAAGACG
CCTCTTCCGCAAGCTCGACAGGACCTTCTCGATGA

Upstream 100 bases:

>100_bases
GCGACAAGGCTGCGATCATGACCTCGCGCGATCCGCGCGAGGTGGCCGCGCGCCTTTACGAGGCTTTGGCATCGGGTGTT
GTCGACAGCCGGGAGGCGGC

Downstream 100 bases:

>100_bases
CAGCTACGATCCTCGTTGCAGATGATGATGCGGCCATCCGCACCGTGCTCAACCAGGCTTTGAGCCGCGCCGGCTATGAC
GTTCGCATCACCTCCAACGC

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTKDTTSPPDQAGGTVAMAVLNAIQNPVVMVDESGFVVFANWEAEAFFGASAPHLARYRISTFIPFGSPLLALIDQVRER
KAPVNEYRVDLSSPRLGQDKLVDLYVAPVLSEPGAVVIVFQERSMADKIDRQLTHRAAARSVTGLASMLAHEIKNPLSGI
RGAAQLLEQSAVDDDRALTRLICDETDRIVSLVDRMEVFSDERPVDRMPVNIHSVLDHVKAVARAGFARNIRVTESYDPS
LPAVYANRDQLVQVFLNLVKNAAEAVGDRPDGEILLTTAYRPGIRLSVAGTREKISLPLEFCVHDNGPGVPADLLPHLFD
PFITTKTNGSGLGLALVAKIIGDHGGIIECDSQNSRTTFRVLMPASKDASLEDASSASSTGPSR

Sequences:

>Translated_384_residues
MTKDTTSPPDQAGGTVAMAVLNAIQNPVVMVDESGFVVFANWEAEAFFGASAPHLARYRISTFIPFGSPLLALIDQVRER
KAPVNEYRVDLSSPRLGQDKLVDLYVAPVLSEPGAVVIVFQERSMADKIDRQLTHRAAARSVTGLASMLAHEIKNPLSGI
RGAAQLLEQSAVDDDRALTRLICDETDRIVSLVDRMEVFSDERPVDRMPVNIHSVLDHVKAVARAGFARNIRVTESYDPS
LPAVYANRDQLVQVFLNLVKNAAEAVGDRPDGEILLTTAYRPGIRLSVAGTREKISLPLEFCVHDNGPGVPADLLPHLFD
PFITTKTNGSGLGLALVAKIIGDHGGIIECDSQNSRTTFRVLMPASKDASLEDASSASSTGPSR
>Mature_383_residues
TKDTTSPPDQAGGTVAMAVLNAIQNPVVMVDESGFVVFANWEAEAFFGASAPHLARYRISTFIPFGSPLLALIDQVRERK
APVNEYRVDLSSPRLGQDKLVDLYVAPVLSEPGAVVIVFQERSMADKIDRQLTHRAAARSVTGLASMLAHEIKNPLSGIR
GAAQLLEQSAVDDDRALTRLICDETDRIVSLVDRMEVFSDERPVDRMPVNIHSVLDHVKAVARAGFARNIRVTESYDPSL
PAVYANRDQLVQVFLNLVKNAAEAVGDRPDGEILLTTAYRPGIRLSVAGTREKISLPLEFCVHDNGPGVPADLLPHLFDP
FITTKTNGSGLGLALVAKIIGDHGGIIECDSQNSRTTFRVLMPASKDASLEDASSASSTGPSR

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=363, Percent_Identity=31.404958677686, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1790436, Length=226, Percent_Identity=33.6283185840708, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788549, Length=348, Percent_Identity=27.2988505747126, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790567, Length=365, Percent_Identity=26.8493150684932, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788393, Length=236, Percent_Identity=28.3898305084746, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789808, Length=273, Percent_Identity=26.7399267399267, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41504; Mature: 41372

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDTTSPPDQAGGTVAMAVLNAIQNPVVMVDESGFVVFANWEAEAFFGASAPHLARYRI
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEECCCCCEEECCCCCHHHHHHH
STFIPFGSPLLALIDQVRERKAPVNEYRVDLSSPRLGQDKLVDLYVAPVLSEPGAVVIVF
HHCCCCCCHHHHHHHHHHHHCCCHHHHEEECCCCCCCCHHHHHHEEEHHHCCCCEEEEEE
QERSMADKIDRQLTHRAAARSVTGLASMLAHEIKNPLSGIRGAAQLLEQSAVDDDRALTR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LICDETDRIVSLVDRMEVFSDERPVDRMPVNIHSVLDHVKAVARAGFARNIRVTESYDPS
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCC
LPAVYANRDQLVQVFLNLVKNAAEAVGDRPDGEILLTTAYRPGIRLSVAGTREKISLPLE
CCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCEECCEE
FCVHDNGPGVPADLLPHLFDPFITTKTNGSGLGLALVAKIIGDHGGIIECDSQNSRTTFR
EEEECCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEE
VLMPASKDASLEDASSASSTGPSR
EEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKDTTSPPDQAGGTVAMAVLNAIQNPVVMVDESGFVVFANWEAEAFFGASAPHLARYRI
CCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEECCCCCEEECCCCCHHHHHHH
STFIPFGSPLLALIDQVRERKAPVNEYRVDLSSPRLGQDKLVDLYVAPVLSEPGAVVIVF
HHCCCCCCHHHHHHHHHHHHCCCHHHHEEECCCCCCCCHHHHHHEEEHHHCCCCEEEEEE
QERSMADKIDRQLTHRAAARSVTGLASMLAHEIKNPLSGIRGAAQLLEQSAVDDDRALTR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LICDETDRIVSLVDRMEVFSDERPVDRMPVNIHSVLDHVKAVARAGFARNIRVTESYDPS
HHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCC
LPAVYANRDQLVQVFLNLVKNAAEAVGDRPDGEILLTTAYRPGIRLSVAGTREKISLPLE
CCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCEECCEE
FCVHDNGPGVPADLLPHLFDPFITTKTNGSGLGLALVAKIIGDHGGIIECDSQNSRTTFR
EEEECCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCEEEE
VLMPASKDASLEDASSASSTGPSR
EEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]