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The map label for this gene is pcm [H]

Identifier: 209549093

GI number: 209549093

Start: 1523437

End: 1524090

Strand: Direct

Name: pcm [H]

Synonym: Rleg2_1492

Alternate gene names: 209549093

Gene position: 1523437-1524090 (Clockwise)

Preceding gene: 209549092

Following gene: 209549094

Centisome position: 33.57

GC content: 60.55

Gene sequence:

>654_bases
TTGACGGCAAGACTGGCCGAGAAAGAGGGCTTTGCGGCGCTCGTCCTCAGATTGCGTGCCGAAGGCATCTCCGATCTCGA
TCTGCTGACCGCGGTCGAGCAGACGCAGCGCTCGCTTTTCGTGCCGCCGCAATTTTACGATGACGCCTATTCGAGCCGGA
CGATCCCGATCGAATGCGGCTCCTTCCTTGAAGGCATCGATTTTGTCGTCCGCATCCTGCATCACCTGAAGCTGAAGCCA
GGACAGCGCGTTCTGGAAATCGGTACCGGCAGCGGCTTTACCGCCGCCGTCATGGGTCGCATGGCTGAGCGCGTTCTGTC
TATCGACCGCTACAAGACGCTGACATCGGCTGCGCAGCGGCGCATGGAATCGCTTAGTCTGCGCAACGTCATCATCCGCC
ACGCAGACGGCAGTGCCGGCATGCAGGGCGAGGGCACCTTCGACCGCATCCTGGTGACGGCGGCCTTCAATGCGATGCCA
CGCTTTTATACCGATCAGCTTGTTTCCGGCGGTTCGATGATCGCGCCGCTGATGATCTCCGAGAACGAATGCCGCATGGT
GCGGCTGACGAAAACCGGCAGCCGTTTCGAACGCGAGGAACTGTTCGAAGCCCCTTATCTGCCGATCGTTCCGCGCCTTG
CCGCGCTGCTATAA

Upstream 100 bases:

>100_bases
TCAAGCATAATAAGATTTCGGTAACGCCTTTGAAACTGGATCTGACCGATTATTCCGTGACGGACCGCGTGGCGCGGGCC
CTGGGATACGGAGCACAGGT

Downstream 100 bases:

>100_bases
GCTCTACGATTTTTCACCCGTAAGCTATGGTTATCAACTTCTCAAAAATATCGCACTGATTCCAGCATCTTAACTGCGTG
GTAATACTAACGCGTTTTAA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTARLAEKEGFAALVLRLRAEGISDLDLLTAVEQTQRSLFVPPQFYDDAYSSRTIPIECGSFLEGIDFVVRILHHLKLKP
GQRVLEIGTGSGFTAAVMGRMAERVLSIDRYKTLTSAAQRRMESLSLRNVIIRHADGSAGMQGEGTFDRILVTAAFNAMP
RFYTDQLVSGGSMIAPLMISENECRMVRLTKTGSRFEREELFEAPYLPIVPRLAALL

Sequences:

>Translated_217_residues
MTARLAEKEGFAALVLRLRAEGISDLDLLTAVEQTQRSLFVPPQFYDDAYSSRTIPIECGSFLEGIDFVVRILHHLKLKP
GQRVLEIGTGSGFTAAVMGRMAERVLSIDRYKTLTSAAQRRMESLSLRNVIIRHADGSAGMQGEGTFDRILVTAAFNAMP
RFYTDQLVSGGSMIAPLMISENECRMVRLTKTGSRFEREELFEAPYLPIVPRLAALL
>Mature_216_residues
TARLAEKEGFAALVLRLRAEGISDLDLLTAVEQTQRSLFVPPQFYDDAYSSRTIPIECGSFLEGIDFVVRILHHLKLKPG
QRVLEIGTGSGFTAAVMGRMAERVLSIDRYKTLTSAAQRRMESLSLRNVIIRHADGSAGMQGEGTFDRILVTAAFNAMPR
FYTDQLVSGGSMIAPLMISENECRMVRLTKTGSRFEREELFEAPYLPIVPRLAALL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=198, Percent_Identity=35.8585858585859, Blast_Score=123, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI193207222, Length=193, Percent_Identity=25.9067357512953, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71983477, Length=192, Percent_Identity=26.0416666666667, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24115; Mature: 23984

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARLAEKEGFAALVLRLRAEGISDLDLLTAVEQTQRSLFVPPQFYDDAYSSRTIPIECG
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCHHHHHHHCCCCCEECHH
SFLEGIDFVVRILHHLKLKPGQRVLEIGTGSGFTAAVMGRMAERVLSIDRYKTLTSAAQR
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMESLSLRNVIIRHADGSAGMQGEGTFDRILVTAAFNAMPRFYTDQLVSGGSMIAPLMIS
HHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEC
ENECRMVRLTKTGSRFEREELFEAPYLPIVPRLAALL
CCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHC
>Mature Secondary Structure 
TARLAEKEGFAALVLRLRAEGISDLDLLTAVEQTQRSLFVPPQFYDDAYSSRTIPIECG
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCHHHHHHHCCCCCEECHH
SFLEGIDFVVRILHHLKLKPGQRVLEIGTGSGFTAAVMGRMAERVLSIDRYKTLTSAAQR
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMESLSLRNVIIRHADGSAGMQGEGTFDRILVTAAFNAMPRFYTDQLVSGGSMIAPLMIS
HHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEEC
ENECRMVRLTKTGSRFEREELFEAPYLPIVPRLAALL
CCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9304864; 11481430 [H]