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The map label for this gene is nasA [H]

Identifier: 209549053

GI number: 209549053

Start: 1472793

End: 1475450

Strand: Reverse

Name: nasA [H]

Synonym: Rleg2_1450

Alternate gene names: 209549053

Gene position: 1475450-1472793 (Counterclockwise)

Preceding gene: 209549054

Following gene: 209549050

Centisome position: 32.51

GC content: 65.43

Gene sequence:

>2658_bases
ATGGCGGTCGAGGTCAAGACCACCTGCCCCTATTGCGGCGTCGGCTGCGGCGTTATCGCCACGGTCGATGAGGCAGGCGC
CGTCAGCGTCAAGGGCGATCCCGAGCATCCGTCGAATTTTGGCCGGCTGTGCTCGAAGGGTTCGGCGCTTGCCGAAACCA
TCGATCTCGACGGCCGGCTTCTTTATCCCGAAATCGCCGGCGCACGCAGCGGCTGGGACGAGGCGCTTAATCTGGTCGCC
CGGCGTTTTTCCGAAACGATCGCCGAACACGGGCCGGATTCCGTCGCCTTCTACGTCTCCGGCCAATTGCTGACCGAGGA
TTATTACCTCGCCAACAAGCTGATGAAGGGCTTTATCGGCTCGGCCAATATCGACACCAATTCAAGGCTCTGCATGTCCT
CGTCCGTCGCCGGGCATCGCCGCGCCTTCGGCGCCGATACGGTGCCCGGAACCTATGAGGATATCGAACTCGCGGATCTC
GTGGTGCTCACCGGCTCCAACCTCGCCTGGTGCCATCCCGTCATCTACCAGCGGCTGGCCGCCGCAAAAGCGGCGCGGCC
GAACATGCGGATCGTCGTCATCGATCCGCGCCGGACGATGACATGCGATATCGCCGATCTGCATCTCGCCATCCGCCCAG
ACGGCGATGTCGCGCTGTTCATGGGATTGCTTGCCCATCTCGCGACAAGCTCCGCGATCGACCAGAATTATATCGCCTCC
CATACGGAAGGTTTCGCCGGCGCCTTTGCTTCAGCCGCAGCACTCGATATCAACGAACTCCTGGAGCGGACCGGCCTGCC
GGCCATGCAGATCCGCGAGTTCTTCCGCCTGTTCGAGACGACGTCGAAGGTGGTGACCTGCTACAGCCAGGGCGTCAACC
AATCCTCCTCCGGCACCGACAAGGTCAATGCCATCCTCAACTGCCATCTGGCGACCGGCCGCATCGGCCGCCCTGGCATG
GGACCCTTCTCGCTGACCGGCCAGCCGAATGCCATGGGCGGGCGCGAGGTCGGCGGTCTTGCCAATATGCTCGCCGCCCA
TATGGCGATCGAAAATGCCGATGACCGCGACCGCGTGCAGCGCTTCTGGAAATCGCCTGTTATCGCCGCCAAACCGGGCC
TGAAGGCGGTCGACATGTTTCGCGCGGTGGCCGACGGGCGCATCAAGGCGCTCTGGATCATCGCCACCAATCCCGTCGTC
TCGATGCCGGATGCCGACAGCGTCGAAAGCGCGATCGCCGCCTGTCCCTTCGTCGTCGTCTCCGACATTCTGCGAGAGAC
GGATACGGCCCGGCACGCAGATGTGCTTCTGCCCTCGCTCGGCTGGGGCGAGAAGGACGGCACCGTCACCAATTCCGAAC
GGCGCATTTCCCGGCAGCGGCCGTTTCTCGACCTGCCCGGCGATGCCAAGGCCGACTGGTGGCAGCTTGCGGAGGTTGCG
CGCCGCATGGGCTTTGCCGCCGCCTTCGATTTCGCTGCGCCGGCCGACATCTTCGCCGAACACGCCGCCCTGTCCGCGTT
CGAAAACAACGGCAGCCGCGATTTCGACATCGGCGCCCGCGTCGGCATGACCGACGCCGCCTATAGCGAGCTGTCACCTT
TCCAATGGCCGCAGGCAGCAGGAGCAGAACCCGGGGTCACCCGCTTCTTCGCCGATGGCGGTTTTTACCATGCCGATGGC
AAGGCGCGCTTCGTCGCGGTGGAGCCGCCGGCAACGGACCGCACCAATGCCGACTTTCCCTTCACGCTGAATACCGGGCG
CATCCGCGACCAGTGGCACACGATGACGCGAACCGGAAAGAGCGCGCGGCTTTCCGCCCATATCGCCGAACCCTTTGCGG
AGATCCATCCGCGCGACGCGATCGAGACCGGCATATCAAGCGCCGGCCTCGTCGAGATCGACAGCCCGCACGGCAAGGCA
ATCGTCCGGGCGCTGGTGACCGACCGCCAGGCGCGCGGCGGCATCTTCGCGCCGATGCACTGGAACGACCAGTTCGCGGC
AAAAGCGCGGATCGACGCCGTGGTGGCACCGATCACCGATCCCGTATCCGGTCAGCCGGCGTCGAAGAATGTCGCCGTCG
CCGTCCGACCCTTCCGCGCCGCCCATTACGGCTTCGCCGTCTCGGCGACAAAACCCGTAACACCTGACGCGGCCTATTGG
GCCCTGGCGAAGGCTGAGGGCGGCTGGCGGCTGGAGCTTGCCTTCGAAGAAGCGGTCGAAGACTGGACGGCATGGTGCCG
GGCGGTTTTTGCCATTCCCGATGAAATCGAGCCGCTCGGTTATGCCGACCGGCAATCCGGCGACCTCAGGCTTGCCTTCT
TCGACGGCGAGACCCTGCTTGCCGCAGTGTTCCTCGCCCGCGAGCCCGTCGCCGTCGCGCGCAACTGGGCGATCTCGCAG
CTCTCCGCCGCGCATGATGATCTGAGGAAACGTTTTGCGCTCGTCGCAGGCCGTCCCGGCGCCGGCAGGCCGGATCCGGG
CGCCACCGTCTGCTCCTGCTTCAGCGTCGGGGTCAACCAGATCGCCGCCGCCGTGCGCGGCGGATGCCACAGCGTCGAGG
CGGTCGGCAAGGAAACGAGCGCCGGAACCAATTGCGGCTCCTGCCGCGGCGAAATCGGCAGGATCATCGATCGCTGTCTT
GCCGCCGCCGCGGAGTGA

Upstream 100 bases:

>100_bases
CGCTCGGCGCCGACCAGGGCGAGGTGCGGACCATACCGGTCCGCAACGAGGACGGCGCGCTGTTCATCGCGCTGGAAAGC
CTGTTGATGGCAGCTGAATA

Downstream 100 bases:

>100_bases
AAGGCAGGACTTATCCGGCCGCCTTCGGCACGATGAACATTTTATGGTCGGCCGAGATGCGGACCGAGATCTCGTCGTAG
GATGACAGCGTCGGATGCGG

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 885; Mature: 884

Protein sequence:

>885_residues
MAVEVKTTCPYCGVGCGVIATVDEAGAVSVKGDPEHPSNFGRLCSKGSALAETIDLDGRLLYPEIAGARSGWDEALNLVA
RRFSETIAEHGPDSVAFYVSGQLLTEDYYLANKLMKGFIGSANIDTNSRLCMSSSVAGHRRAFGADTVPGTYEDIELADL
VVLTGSNLAWCHPVIYQRLAAAKAARPNMRIVVIDPRRTMTCDIADLHLAIRPDGDVALFMGLLAHLATSSAIDQNYIAS
HTEGFAGAFASAAALDINELLERTGLPAMQIREFFRLFETTSKVVTCYSQGVNQSSSGTDKVNAILNCHLATGRIGRPGM
GPFSLTGQPNAMGGREVGGLANMLAAHMAIENADDRDRVQRFWKSPVIAAKPGLKAVDMFRAVADGRIKALWIIATNPVV
SMPDADSVESAIAACPFVVVSDILRETDTARHADVLLPSLGWGEKDGTVTNSERRISRQRPFLDLPGDAKADWWQLAEVA
RRMGFAAAFDFAAPADIFAEHAALSAFENNGSRDFDIGARVGMTDAAYSELSPFQWPQAAGAEPGVTRFFADGGFYHADG
KARFVAVEPPATDRTNADFPFTLNTGRIRDQWHTMTRTGKSARLSAHIAEPFAEIHPRDAIETGISSAGLVEIDSPHGKA
IVRALVTDRQARGGIFAPMHWNDQFAAKARIDAVVAPITDPVSGQPASKNVAVAVRPFRAAHYGFAVSATKPVTPDAAYW
ALAKAEGGWRLELAFEEAVEDWTAWCRAVFAIPDEIEPLGYADRQSGDLRLAFFDGETLLAAVFLAREPVAVARNWAISQ
LSAAHDDLRKRFALVAGRPGAGRPDPGATVCSCFSVGVNQIAAAVRGGCHSVEAVGKETSAGTNCGSCRGEIGRIIDRCL
AAAAE

Sequences:

>Translated_885_residues
MAVEVKTTCPYCGVGCGVIATVDEAGAVSVKGDPEHPSNFGRLCSKGSALAETIDLDGRLLYPEIAGARSGWDEALNLVA
RRFSETIAEHGPDSVAFYVSGQLLTEDYYLANKLMKGFIGSANIDTNSRLCMSSSVAGHRRAFGADTVPGTYEDIELADL
VVLTGSNLAWCHPVIYQRLAAAKAARPNMRIVVIDPRRTMTCDIADLHLAIRPDGDVALFMGLLAHLATSSAIDQNYIAS
HTEGFAGAFASAAALDINELLERTGLPAMQIREFFRLFETTSKVVTCYSQGVNQSSSGTDKVNAILNCHLATGRIGRPGM
GPFSLTGQPNAMGGREVGGLANMLAAHMAIENADDRDRVQRFWKSPVIAAKPGLKAVDMFRAVADGRIKALWIIATNPVV
SMPDADSVESAIAACPFVVVSDILRETDTARHADVLLPSLGWGEKDGTVTNSERRISRQRPFLDLPGDAKADWWQLAEVA
RRMGFAAAFDFAAPADIFAEHAALSAFENNGSRDFDIGARVGMTDAAYSELSPFQWPQAAGAEPGVTRFFADGGFYHADG
KARFVAVEPPATDRTNADFPFTLNTGRIRDQWHTMTRTGKSARLSAHIAEPFAEIHPRDAIETGISSAGLVEIDSPHGKA
IVRALVTDRQARGGIFAPMHWNDQFAAKARIDAVVAPITDPVSGQPASKNVAVAVRPFRAAHYGFAVSATKPVTPDAAYW
ALAKAEGGWRLELAFEEAVEDWTAWCRAVFAIPDEIEPLGYADRQSGDLRLAFFDGETLLAAVFLAREPVAVARNWAISQ
LSAAHDDLRKRFALVAGRPGAGRPDPGATVCSCFSVGVNQIAAAVRGGCHSVEAVGKETSAGTNCGSCRGEIGRIIDRCL
AAAAE
>Mature_884_residues
AVEVKTTCPYCGVGCGVIATVDEAGAVSVKGDPEHPSNFGRLCSKGSALAETIDLDGRLLYPEIAGARSGWDEALNLVAR
RFSETIAEHGPDSVAFYVSGQLLTEDYYLANKLMKGFIGSANIDTNSRLCMSSSVAGHRRAFGADTVPGTYEDIELADLV
VLTGSNLAWCHPVIYQRLAAAKAARPNMRIVVIDPRRTMTCDIADLHLAIRPDGDVALFMGLLAHLATSSAIDQNYIASH
TEGFAGAFASAAALDINELLERTGLPAMQIREFFRLFETTSKVVTCYSQGVNQSSSGTDKVNAILNCHLATGRIGRPGMG
PFSLTGQPNAMGGREVGGLANMLAAHMAIENADDRDRVQRFWKSPVIAAKPGLKAVDMFRAVADGRIKALWIIATNPVVS
MPDADSVESAIAACPFVVVSDILRETDTARHADVLLPSLGWGEKDGTVTNSERRISRQRPFLDLPGDAKADWWQLAEVAR
RMGFAAAFDFAAPADIFAEHAALSAFENNGSRDFDIGARVGMTDAAYSELSPFQWPQAAGAEPGVTRFFADGGFYHADGK
ARFVAVEPPATDRTNADFPFTLNTGRIRDQWHTMTRTGKSARLSAHIAEPFAEIHPRDAIETGISSAGLVEIDSPHGKAI
VRALVTDRQARGGIFAPMHWNDQFAAKARIDAVVAPITDPVSGQPASKNVAVAVRPFRAAHYGFAVSATKPVTPDAAYWA
LAKAEGGWRLELAFEEAVEDWTAWCRAVFAIPDEIEPLGYADRQSGDLRLAFFDGETLLAAVFLAREPVAVARNWAISQL
SAAHDDLRKRFALVAGRPGAGRPDPGATVCSCFSVGVNQIAAAVRGGCHSVEAVGKETSAGTNCGSCRGEIGRIIDRCLA
AAAE

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=794, Percent_Identity=28.9672544080605, Blast_Score=325, Evalue=8e-90,
Organism=Escherichia coli, GI3868721, Length=710, Percent_Identity=29.0140845070423, Blast_Score=288, Evalue=1e-78,
Organism=Escherichia coli, GI3868719, Length=610, Percent_Identity=23.4426229508197, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI3868720, Length=265, Percent_Identity=30.9433962264151, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1787778, Length=427, Percent_Identity=25.7611241217799, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787870, Length=527, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 94565; Mature: 94433

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEVKTTCPYCGVGCGVIATVDEAGAVSVKGDPEHPSNFGRLCSKGSALAETIDLDGRL
CEEEEECCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHCCCCHHHHHCCCCCEE
LYPEIAGARSGWDEALNLVARRFSETIAEHGPDSVAFYVSGQLLTEDYYLANKLMKGFIG
ECHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEECHHHHHHHHHHHHHCC
SANIDTNSRLCMSSSVAGHRRAFGADTVPGTYEDIELADLVVLTGSNLAWCHPVIYQRLA
CCCCCCCCCEEECCCCCCHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHH
AAKAARPNMRIVVIDPRRTMTCDIADLHLAIRPDGDVALFMGLLAHLATSSAIDQNYIAS
HHHHCCCCCEEEEECCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCHHHHHH
HTEGFAGAFASAAALDINELLERTGLPAMQIREFFRLFETTSKVVTCYSQGVNQSSSGTD
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
KVNAILNCHLATGRIGRPGMGPFSLTGQPNAMGGREVGGLANMLAAHMAIENADDRDRVQ
HEEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
RFWKSPVIAAKPGLKAVDMFRAVADGRIKALWIIATNPVVSMPDADSVESAIAACPFVVV
HHHHCCCEEECCCCHHHHHHHHHHCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHH
SDILRETDTARHADVLLPSLGWGEKDGTVTNSERRISRQRPFLDLPGDAKADWWQLAEVA
HHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEECCCCCCHHHHHHHHHH
RRMGFAAAFDFAAPADIFAEHAALSAFENNGSRDFDIGARVGMTDAAYSELSPFQWPQAA
HHHCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
GAEPGVTRFFADGGFYHADGKARFVAVEPPATDRTNADFPFTLNTGRIRDQWHTMTRTGK
CCCCCCEEEEECCCEEECCCCEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCC
SARLSAHIAEPFAEIHPRDAIETGISSAGLVEIDSPHGKAIVRALVTDRQARGGIFAPMH
CCEEHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEECC
WNDQFAAKARIDAVVAPITDPVSGQPASKNVAVAVRPFRAAHYGFAVSATKPVTPDAAYW
CCCCHHHHHHHHEEEECCCCCCCCCCCCCCEEEEEECCHHHHCCEEEECCCCCCCCHHHE
ALAKAEGGWRLELAFEEAVEDWTAWCRAVFAIPDEIEPLGYADRQSGDLRLAFFDGETLL
EEEECCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
AAVFLAREPVAVARNWAISQLSAAHDDLRKRFALVAGRPGAGRPDPGATVCSCFSVGVNQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
IAAAVRGGCHSVEAVGKETSAGTNCGSCRGEIGRIIDRCLAAAAE
HHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVEVKTTCPYCGVGCGVIATVDEAGAVSVKGDPEHPSNFGRLCSKGSALAETIDLDGRL
EEEEECCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHCCCCHHHHHCCCCCEE
LYPEIAGARSGWDEALNLVARRFSETIAEHGPDSVAFYVSGQLLTEDYYLANKLMKGFIG
ECHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEECHHHHHHHHHHHHHCC
SANIDTNSRLCMSSSVAGHRRAFGADTVPGTYEDIELADLVVLTGSNLAWCHPVIYQRLA
CCCCCCCCCEEECCCCCCHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHH
AAKAARPNMRIVVIDPRRTMTCDIADLHLAIRPDGDVALFMGLLAHLATSSAIDQNYIAS
HHHHCCCCCEEEEECCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCHHHHHH
HTEGFAGAFASAAALDINELLERTGLPAMQIREFFRLFETTSKVVTCYSQGVNQSSSGTD
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
KVNAILNCHLATGRIGRPGMGPFSLTGQPNAMGGREVGGLANMLAAHMAIENADDRDRVQ
HEEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
RFWKSPVIAAKPGLKAVDMFRAVADGRIKALWIIATNPVVSMPDADSVESAIAACPFVVV
HHHHCCCEEECCCCHHHHHHHHHHCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHH
SDILRETDTARHADVLLPSLGWGEKDGTVTNSERRISRQRPFLDLPGDAKADWWQLAEVA
HHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEECCCCCCHHHHHHHHHH
RRMGFAAAFDFAAPADIFAEHAALSAFENNGSRDFDIGARVGMTDAAYSELSPFQWPQAA
HHHCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
GAEPGVTRFFADGGFYHADGKARFVAVEPPATDRTNADFPFTLNTGRIRDQWHTMTRTGK
CCCCCCEEEEECCCEEECCCCEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCC
SARLSAHIAEPFAEIHPRDAIETGISSAGLVEIDSPHGKAIVRALVTDRQARGGIFAPMH
CCEEHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEECC
WNDQFAAKARIDAVVAPITDPVSGQPASKNVAVAVRPFRAAHYGFAVSATKPVTPDAAYW
CCCCHHHHHHHHEEEECCCCCCCCCCCCCCEEEEEECCHHHHCCEEEECCCCCCCCHHHE
ALAKAEGGWRLELAFEEAVEDWTAWCRAVFAIPDEIEPLGYADRQSGDLRLAFFDGETLL
EEEECCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
AAVFLAREPVAVARNWAISQLSAAHDDLRKRFALVAGRPGAGRPDPGATVCSCFSVGVNQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
IAAAVRGGCHSVEAVGKETSAGTNCGSCRGEIGRIIDRCLAAAAE
HHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]