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The map label for this gene is cfa [H]

Identifier: 209549044

GI number: 209549044

Start: 1464769

End: 1466028

Strand: Reverse

Name: cfa [H]

Synonym: Rleg2_1441

Alternate gene names: 209549044

Gene position: 1466028-1464769 (Counterclockwise)

Preceding gene: 209549045

Following gene: 209549041

Centisome position: 32.31

GC content: 62.22

Gene sequence:

>1260_bases
ATGAGCAAGGCGCAGGATTGGAATGTGCTGGCCCGTGACGCGGTGACGCTGACGGCCGAAAATATATCCCGTCTGGTGAA
GGGGCTGCCGTTCAAGGCGAGGCTGGCGCTGCGGGGGCTTCTGCGCATGCAGCACGGCTCGCTTGCGGTCACCCTGCCCG
ACGGCCGCAGGCTGCTGATCGAGGGCAAGCAGGCCGGGCCGAAGGCGGCTCTCAGCCTCAACAACTGGAACCTTGCCTAT
CGAGCCCTGACGAGCGGCACGATCGGGGTTGCCGAAACCTATATGGACGGCGACTGGGACAGCCCCGACATTGCCGCCTT
CCTCGAACTTTTCCTCGTCAACGGCGAGGCCGTCTACAGCTATTCCAATGGCAAGGGCGGCATTGGCCGCCTCGTCGAGC
GCGTCCGCCATTGGATGAACACCAATACCAAGACGGGCTCGAAGCGTAACATTTCCGCCCATTACGATCTCGGCAATGAT
TTCTACAGGCAGTGGCTCGATCCGAGCATGACCTATTCCTCGGCACTCTATTCGACAGGGGCCAACGACCTGCAATCGGC
GCAGAATGCCAAATACCGCGCGCTCGCCGAGGCGACCGGCATCAGACCCGGCGATCACGTGCTGGAGATCGGCTGCGGCT
GGGGCGGGTTTGCCGAATTTGCAGCAGGCGAACTGAACTGCAAGGTGACCGGGCTGACGATCAGCCGCGAGCAGCTGGCC
TTTGCCGAGGAGCGCATCCGCAAGGCCGGTCTCGGCGACCGCGTCGAATTCCGTTTCCAGGATTACCGCGACGAGACCGG
GCTTTACGACCGGATCGTCTCGATCGAGATGTTCGAAGCGGTCGGGGAGAAATACTGGCCGTCCTATTTCTCCAAGCTCC
GGCAATGTCTGAAGCCGGGCGGCAAGGCCGGCCTGCAGATCATCACCATCCGGCCGGAGGCCTTCGACCAGTATCGCAGC
AACCCCGACTTCATCCAGAAATATGTCTTTCCCGGCGGCATGCTGCCGACGCGCAACCATCTGGCGGAACTTGCCGGCAA
GGTCGACCTGTCACTGGTCAGGGACTTCGGCTTCGGGCTCGACTATGCCCGCACGCTTGCCGAATGGCGCGAACGCTTCT
GGTCTGTCTGGGAGCGCATCCGCCCGATGGGCTTCGACGAGCGTTTCAAGCGGCTCTGGGAATTCTATCTGTTCTATTGC
GAGGCCGGCTTCCGCGCCCGCAATATCGATGTGCGCCAAGTGGTGTTCTCGCGCAGCTGA

Upstream 100 bases:

>100_bases
TTATGCAGTAGCCTTGCCTAATAATGGCCGGAGGCCGCCCGAACATCGTCACGGATGTATCGTCGTCCCTAACCGGCAAA
AAAATGCTGGAGGAGGAAAG

Downstream 100 bases:

>100_bases
TCATTCCGCCGGATGCGGTGTCGCCGGATGGTGACCAGCCGATGACGGCCGCTCGCGCCAGCCGGTCACGCGGTTCACCA
CCATGAAATAGGCGCTGTAG

Product: Cyclopropane-fatty-acyl-phospholipid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSKAQDWNVLARDAVTLTAENISRLVKGLPFKARLALRGLLRMQHGSLAVTLPDGRRLLIEGKQAGPKAALSLNNWNLAY
RALTSGTIGVAETYMDGDWDSPDIAAFLELFLVNGEAVYSYSNGKGGIGRLVERVRHWMNTNTKTGSKRNISAHYDLGND
FYRQWLDPSMTYSSALYSTGANDLQSAQNAKYRALAEATGIRPGDHVLEIGCGWGGFAEFAAGELNCKVTGLTISREQLA
FAEERIRKAGLGDRVEFRFQDYRDETGLYDRIVSIEMFEAVGEKYWPSYFSKLRQCLKPGGKAGLQIITIRPEAFDQYRS
NPDFIQKYVFPGGMLPTRNHLAELAGKVDLSLVRDFGFGLDYARTLAEWRERFWSVWERIRPMGFDERFKRLWEFYLFYC
EAGFRARNIDVRQVVFSRS

Sequences:

>Translated_419_residues
MSKAQDWNVLARDAVTLTAENISRLVKGLPFKARLALRGLLRMQHGSLAVTLPDGRRLLIEGKQAGPKAALSLNNWNLAY
RALTSGTIGVAETYMDGDWDSPDIAAFLELFLVNGEAVYSYSNGKGGIGRLVERVRHWMNTNTKTGSKRNISAHYDLGND
FYRQWLDPSMTYSSALYSTGANDLQSAQNAKYRALAEATGIRPGDHVLEIGCGWGGFAEFAAGELNCKVTGLTISREQLA
FAEERIRKAGLGDRVEFRFQDYRDETGLYDRIVSIEMFEAVGEKYWPSYFSKLRQCLKPGGKAGLQIITIRPEAFDQYRS
NPDFIQKYVFPGGMLPTRNHLAELAGKVDLSLVRDFGFGLDYARTLAEWRERFWSVWERIRPMGFDERFKRLWEFYLFYC
EAGFRARNIDVRQVVFSRS
>Mature_418_residues
SKAQDWNVLARDAVTLTAENISRLVKGLPFKARLALRGLLRMQHGSLAVTLPDGRRLLIEGKQAGPKAALSLNNWNLAYR
ALTSGTIGVAETYMDGDWDSPDIAAFLELFLVNGEAVYSYSNGKGGIGRLVERVRHWMNTNTKTGSKRNISAHYDLGNDF
YRQWLDPSMTYSSALYSTGANDLQSAQNAKYRALAEATGIRPGDHVLEIGCGWGGFAEFAAGELNCKVTGLTISREQLAF
AEERIRKAGLGDRVEFRFQDYRDETGLYDRIVSIEMFEAVGEKYWPSYFSKLRQCLKPGGKAGLQIITIRPEAFDQYRSN
PDFIQKYVFPGGMLPTRNHLAELAGKVDLSLVRDFGFGLDYARTLAEWRERFWSVWERIRPMGFDERFKRLWEFYLFYCE
AGFRARNIDVRQVVFSRS

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [H]

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=350, Percent_Identity=37.7142857142857, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI133904226, Length=386, Percent_Identity=39.119170984456, Blast_Score=257, Evalue=7e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 47543; Mature: 47411

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAQDWNVLARDAVTLTAENISRLVKGLPFKARLALRGLLRMQHGSLAVTLPDGRRLLI
CCCCCCCCHHHHHHHEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
EGKQAGPKAALSLNNWNLAYRALTSGTIGVAETYMDGDWDSPDIAAFLELFLVNGEAVYS
ECCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEE
YSNGKGGIGRLVERVRHWMNTNTKTGSKRNISAHYDLGNDFYRQWLDPSMTYSSALYSTG
ECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHCC
ANDLQSAQNAKYRALAEATGIRPGDHVLEIGCGWGGFAEFAAGELNCKVTGLTISREQLA
CHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCEEEEEEEEECHHHHH
FAEERIRKAGLGDRVEFRFQDYRDETGLYDRIVSIEMFEAVGEKYWPSYFSKLRQCLKPG
HHHHHHHHCCCCCCEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCC
GKAGLQIITIRPEAFDQYRSNPDFIQKYVFPGGMLPTRNHLAELAGKVDLSLVRDFGFGL
CCCCEEEEEECCHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
DYARTLAEWRERFWSVWERIRPMGFDERFKRLWEFYLFYCEAGFRARNIDVRQVVFSRS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHCCC
>Mature Secondary Structure 
SKAQDWNVLARDAVTLTAENISRLVKGLPFKARLALRGLLRMQHGSLAVTLPDGRRLLI
CCCCCCCHHHHHHHEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
EGKQAGPKAALSLNNWNLAYRALTSGTIGVAETYMDGDWDSPDIAAFLELFLVNGEAVYS
ECCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEE
YSNGKGGIGRLVERVRHWMNTNTKTGSKRNISAHYDLGNDFYRQWLDPSMTYSSALYSTG
ECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHCC
ANDLQSAQNAKYRALAEATGIRPGDHVLEIGCGWGGFAEFAAGELNCKVTGLTISREQLA
CHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCEEEEEEEEECHHHHH
FAEERIRKAGLGDRVEFRFQDYRDETGLYDRIVSIEMFEAVGEKYWPSYFSKLRQCLKPG
HHHHHHHHCCCCCCEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCC
GKAGLQIITIRPEAFDQYRSNPDFIQKYVFPGGMLPTRNHLAELAGKVDLSLVRDFGFGL
CCCCEEEEEECCHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
DYARTLAEWRERFWSVWERIRPMGFDERFKRLWEFYLFYCEAGFRARNIDVRQVVFSRS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]