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The map label for this gene is cpdB [H]

Identifier: 209549032

GI number: 209549032

Start: 1453355

End: 1455343

Strand: Reverse

Name: cpdB [H]

Synonym: Rleg2_1429

Alternate gene names: 209549032

Gene position: 1455343-1453355 (Counterclockwise)

Preceding gene: 209549033

Following gene: 209549030

Centisome position: 32.07

GC content: 60.73

Gene sequence:

>1989_bases
ATGTCTTCCATTTTCGATGTGGGTGTGATGAGTCGCCGTTCCCTGCTTGGCGGCCTTGCCGCCACCTCCGCCCTGGTGCT
GCTGCACCCCTTCAGCGCGCGCGCCAGCGCCAACCAGGCCCATCTCCGGCTGATGGAAACGACGGATATTCACGTCAACG
TCTTCCCCTATGATTATTACGCCGACAAGCCGAACGACACGATGGGGCTTTCCCGCACCGGGACGATCATCGACAATATC
CGCGCCGAGGCCGTCAACTCGCTGCTGATTGACAATGGCGACGTGCTGCAGGGCAATCCGATGGGCGACTACATGGCCTA
CCAGCACGGCATGAAGGACGGCGACGTCCATCCCGTGATCAAGGCGATGAACACACTCGGCTACACGGTGGGAACGCTCG
GCAATCACGAGTTCAATTACGGGCTCGACTTCATGTTCAAGGTGCTATCGGGCGCGAACTTCCCCTTCGTCTGCGCCAAT
CTGACCAAGGGCCAGCTCGCCTCGGACCCAAAGCAGGACGATCTGTTCTTCAAGCCGTACATGATCGTGGAAAAACAGAT
CAAGGACGGCGCCGGCAATGAGAGCCCTGTCAAGATCGGCTTCATCGGCTTCGTGCCGCCGCAGATTATGCTCTGGGACA
TCAAGAACCTCGAAGGCAAGGCGCAGACACGTGATATCGTCGAGGCCGCCAAGGCCTGGGTTCCGGCCATGAAGGAAGCC
GGCGCCGATATCGTCATCGCGCTTTCCCATTCCGGCATCGATGGAGCCGCGCCGTCCGAAAAGATGGAAAACGCCTCGCT
GCATCTCGCCGCCGTCGAGGGGATCGACGCGATCTTCACCGGCCATCAGCATCTCGTCTTCCCCGGGCCGAAGAGCTGGG
ACGGCATTGCCAATGCCGATCCGGTCAAAGGCACGCTGCATGGCAAACCCGCCGTGATGGCAGGCTTCTGGGGCTCGCAT
CTCGGCCTTATCGACCTGCTGCTGGAAAAGGACGGCAACAGCTGGAAGATCGTCGACTTCACCTCGGAAGCACGGCCGAT
CTACCATCGTGACGACAAGAAGAAGGTGGTTGCCGACTACGCCGACAAGAAGGAAGTGGTCGAGGCGGCAAAGGCCGAAC
ACGAGGCGACGCTTGCCTATGTCCGCACCCCCGTCGGCAAGACGTCGGCGCCGCTCTATTCCTACTTCGCGCTCGTCGCC
GACGATCCTTCCGTGCAGGTCGTCAGCCAGGCTCAGACCTGGTACATCAAGCAGATGCTGGCCGATACCGCGTTCAACGA
TCTGCCGGTGCTTTCAGCCGCTGCCCCCTTCAAGGCCGGCGGCCGCGGCGGCGCCGATTACTATACCGACGTTCCAGCCG
GCGATATCGCCATCAAGAACGTCTCCGACCTCTATCTCTACCCGAATACGGTGCAGGCTGTCGCCATCACCGGCGCCCAG
GTGAAGAACTGGCTGGAAATGTCGGCCGGCATGTTCAATCATATCGAGCCCGGCTCGAAGGATGCGCCGCTGCTCAACAA
CGACTTCCCGTCCTATAATTTCGACGTCATCGACGGCGTGACCTACCAGATCGATCTATCGCAGCCGCCGAAATATGATT
CCTCCGGCAAGGCGATCAATCCGGATGCCAACCGCATCCAGAACCTCGCCTTCAGCGGCAAGCCGATCGATCCCGCCCAG
AAATTCGTCGTCGTCACCAACAATTATCGCGCCGGCGGCGGCGGCAGTTTCCCGGAGATCGCTGCTGATAAGGTGATCTT
CCAGGCGCCGGACACCAACCGTGACGTCATCGTCCGCTATGTCCACGACCAGGGCACGATCAATCCGTCGGCCGATGCGA
ACTGGACCTTCAAGCCGCTGCCGGGCACGACGGCGACCTTCGAGAGCGGCCCGAAGGCCAAGCAGTTCCTCGCTGCGGTC
AAGAGCGTCAAGATCGAAGATGCCGGCGACGGTGCCGACGGCTTCTCGAAGTTCAGGCTGGTGCTTTAA

Upstream 100 bases:

>100_bases
TCATTGTTTTGCCGTCCTGTCATTTTTGCAGTGTACTCCTCGCCAAATCGTCCTAATTCTGACGGGCCGGACGATGCATG
ATCCTTCAAGGAGAGTTTCG

Downstream 100 bases:

>100_bases
GAATAAAAGGAAGGCGCGTCGGCCGCGCCTTCTTTATTCAGCCGCGAGCGGGATTGCGGGTTCACGCAGTTCCGCGATCT
GGGCGTGGAAATCCGCCTGG

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MSSIFDVGVMSRRSLLGGLAATSALVLLHPFSARASANQAHLRLMETTDIHVNVFPYDYYADKPNDTMGLSRTGTIIDNI
RAEAVNSLLIDNGDVLQGNPMGDYMAYQHGMKDGDVHPVIKAMNTLGYTVGTLGNHEFNYGLDFMFKVLSGANFPFVCAN
LTKGQLASDPKQDDLFFKPYMIVEKQIKDGAGNESPVKIGFIGFVPPQIMLWDIKNLEGKAQTRDIVEAAKAWVPAMKEA
GADIVIALSHSGIDGAAPSEKMENASLHLAAVEGIDAIFTGHQHLVFPGPKSWDGIANADPVKGTLHGKPAVMAGFWGSH
LGLIDLLLEKDGNSWKIVDFTSEARPIYHRDDKKKVVADYADKKEVVEAAKAEHEATLAYVRTPVGKTSAPLYSYFALVA
DDPSVQVVSQAQTWYIKQMLADTAFNDLPVLSAAAPFKAGGRGGADYYTDVPAGDIAIKNVSDLYLYPNTVQAVAITGAQ
VKNWLEMSAGMFNHIEPGSKDAPLLNNDFPSYNFDVIDGVTYQIDLSQPPKYDSSGKAINPDANRIQNLAFSGKPIDPAQ
KFVVVTNNYRAGGGGSFPEIAADKVIFQAPDTNRDVIVRYVHDQGTINPSADANWTFKPLPGTTATFESGPKAKQFLAAV
KSVKIEDAGDGADGFSKFRLVL

Sequences:

>Translated_662_residues
MSSIFDVGVMSRRSLLGGLAATSALVLLHPFSARASANQAHLRLMETTDIHVNVFPYDYYADKPNDTMGLSRTGTIIDNI
RAEAVNSLLIDNGDVLQGNPMGDYMAYQHGMKDGDVHPVIKAMNTLGYTVGTLGNHEFNYGLDFMFKVLSGANFPFVCAN
LTKGQLASDPKQDDLFFKPYMIVEKQIKDGAGNESPVKIGFIGFVPPQIMLWDIKNLEGKAQTRDIVEAAKAWVPAMKEA
GADIVIALSHSGIDGAAPSEKMENASLHLAAVEGIDAIFTGHQHLVFPGPKSWDGIANADPVKGTLHGKPAVMAGFWGSH
LGLIDLLLEKDGNSWKIVDFTSEARPIYHRDDKKKVVADYADKKEVVEAAKAEHEATLAYVRTPVGKTSAPLYSYFALVA
DDPSVQVVSQAQTWYIKQMLADTAFNDLPVLSAAAPFKAGGRGGADYYTDVPAGDIAIKNVSDLYLYPNTVQAVAITGAQ
VKNWLEMSAGMFNHIEPGSKDAPLLNNDFPSYNFDVIDGVTYQIDLSQPPKYDSSGKAINPDANRIQNLAFSGKPIDPAQ
KFVVVTNNYRAGGGGSFPEIAADKVIFQAPDTNRDVIVRYVHDQGTINPSADANWTFKPLPGTTATFESGPKAKQFLAAV
KSVKIEDAGDGADGFSKFRLVL
>Mature_661_residues
SSIFDVGVMSRRSLLGGLAATSALVLLHPFSARASANQAHLRLMETTDIHVNVFPYDYYADKPNDTMGLSRTGTIIDNIR
AEAVNSLLIDNGDVLQGNPMGDYMAYQHGMKDGDVHPVIKAMNTLGYTVGTLGNHEFNYGLDFMFKVLSGANFPFVCANL
TKGQLASDPKQDDLFFKPYMIVEKQIKDGAGNESPVKIGFIGFVPPQIMLWDIKNLEGKAQTRDIVEAAKAWVPAMKEAG
ADIVIALSHSGIDGAAPSEKMENASLHLAAVEGIDAIFTGHQHLVFPGPKSWDGIANADPVKGTLHGKPAVMAGFWGSHL
GLIDLLLEKDGNSWKIVDFTSEARPIYHRDDKKKVVADYADKKEVVEAAKAEHEATLAYVRTPVGKTSAPLYSYFALVAD
DPSVQVVSQAQTWYIKQMLADTAFNDLPVLSAAAPFKAGGRGGADYYTDVPAGDIAIKNVSDLYLYPNTVQAVAITGAQV
KNWLEMSAGMFNHIEPGSKDAPLLNNDFPSYNFDVIDGVTYQIDLSQPPKYDSSGKAINPDANRIQNLAFSGKPIDPAQK
FVVVTNNYRAGGGGSFPEIAADKVIFQAPDTNRDVIVRYVHDQGTINPSADANWTFKPLPGTTATFESGPKAKQFLAAVK
SVKIEDAGDGADGFSKFRLVL

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=624, Percent_Identity=47.7564102564103, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1786687, Length=624, Percent_Identity=21.9551282051282, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 71516; Mature: 71384

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIFDVGVMSRRSLLGGLAATSALVLLHPFSARASANQAHLRLMETTDIHVNVFPYDYY
CCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCEEEEEEEECEEEEEEEEEEEE
ADKPNDTMGLSRTGTIIDNIRAEAVNSLLIDNGDVLQGNPMGDYMAYQHGMKDGDVHPVI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCEEECCCCCHHHHHHCCCCCCCHHHHH
KAMNTLGYTVGTLGNHEFNYGLDFMFKVLSGANFPFVCANLTKGQLASDPKQDDLFFKPY
HHHHHHCEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEECCH
MIVEKQIKDGAGNESPVKIGFIGFVPPQIMLWDIKNLEGKAQTRDIVEAAKAWVPAMKEA
HHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GADIVIALSHSGIDGAAPSEKMENASLHLAAVEGIDAIFTGHQHLVFPGPKSWDGIANAD
CCCEEEEEECCCCCCCCCHHHHCCCCEEEEEECCCCEEEECCEEEEECCCCCCCCCCCCC
PVKGTLHGKPAVMAGFWGSHLGLIDLLLEKDGNSWKIVDFTSEARPIYHRDDKKKVVADY
CCCEEECCCCEEEEECCCCHHHHEEHEEECCCCCEEEEEECCCCCCCEECCCCHHHHHHH
ADKKEVVEAAKAEHEATLAYVRTPVGKTSAPLYSYFALVADDPSVQVVSQAQTWYIKQML
CCHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHEEEEEECCCCEEEEHHHHHHHHHHHH
ADTAFNDLPVLSAAAPFKAGGRGGADYYTDVPAGDIAIKNVSDLYLYPNTVQAVAITGAQ
HHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCEEEEEECHHH
VKNWLEMSAGMFNHIEPGSKDAPLLNNDFPSYNFDVIDGVTYQIDLSQPPKYDSSGKAIN
HHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCEEC
PDANRIQNLAFSGKPIDPAQKFVVVTNNYRAGGGGSFPEIAADKVIFQAPDTNRDVIVRY
CCHHHHHHHCCCCCCCCCCCEEEEEECCEECCCCCCCCCHHCCCEEEECCCCCCCEEEEE
VHDQGTINPSADANWTFKPLPGTTATFESGPKAKQFLAAVKSVKIEDAGDGADGFSKFRL
EECCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCEEE
VL
EC
>Mature Secondary Structure 
SSIFDVGVMSRRSLLGGLAATSALVLLHPFSARASANQAHLRLMETTDIHVNVFPYDYY
CCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCEEEEEEEECEEEEEEEEEEEE
ADKPNDTMGLSRTGTIIDNIRAEAVNSLLIDNGDVLQGNPMGDYMAYQHGMKDGDVHPVI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCEEECCCCCHHHHHHCCCCCCCHHHHH
KAMNTLGYTVGTLGNHEFNYGLDFMFKVLSGANFPFVCANLTKGQLASDPKQDDLFFKPY
HHHHHHCEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEECCH
MIVEKQIKDGAGNESPVKIGFIGFVPPQIMLWDIKNLEGKAQTRDIVEAAKAWVPAMKEA
HHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
GADIVIALSHSGIDGAAPSEKMENASLHLAAVEGIDAIFTGHQHLVFPGPKSWDGIANAD
CCCEEEEEECCCCCCCCCHHHHCCCCEEEEEECCCCEEEECCEEEEECCCCCCCCCCCCC
PVKGTLHGKPAVMAGFWGSHLGLIDLLLEKDGNSWKIVDFTSEARPIYHRDDKKKVVADY
CCCEEECCCCEEEEECCCCHHHHEEHEEECCCCCEEEEEECCCCCCCEECCCCHHHHHHH
ADKKEVVEAAKAEHEATLAYVRTPVGKTSAPLYSYFALVADDPSVQVVSQAQTWYIKQML
CCHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHEEEEEECCCCEEEEHHHHHHHHHHHH
ADTAFNDLPVLSAAAPFKAGGRGGADYYTDVPAGDIAIKNVSDLYLYPNTVQAVAITGAQ
HHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCEEEEEECHHH
VKNWLEMSAGMFNHIEPGSKDAPLLNNDFPSYNFDVIDGVTYQIDLSQPPKYDSSGKAIN
HHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCEEC
PDANRIQNLAFSGKPIDPAQKFVVVTNNYRAGGGGSFPEIAADKVIFQAPDTNRDVIVRY
CCHHHHHHHCCCCCCCCCCCEEEEEECCEECCCCCCCCCHHCCCEEEECCCCCCCEEEEE
VHDQGTINPSADANWTFKPLPGTTATFESGPKAKQFLAAVKSVKIEDAGDGADGFSKFRL
EECCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCEEE
VL
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]