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The map label for this gene is azoR1 [H]

Identifier: 209549030

GI number: 209549030

Start: 1451601

End: 1452215

Strand: Reverse

Name: azoR1 [H]

Synonym: Rleg2_1427

Alternate gene names: 209549030

Gene position: 1452215-1451601 (Counterclockwise)

Preceding gene: 209549032

Following gene: 209549028

Centisome position: 32.0

GC content: 62.28

Gene sequence:

>615_bases
ATGTCGTCCATCCTTCTTCTGACGTCTAGCCCGCGTGCCGAATCGCTCTCGACGCCGATCGCCGTCGAGCTCGCCGAGAA
GCTGAAGAACCAGAATCCGGGCAGCTTTCTCGTTCGCCGCGACCTTGCTGCCAGCCCGCTGCCGCATATCGACGACCTCT
TCACCGGCGCGATCCGCAAGCCGGCCGAGGCCCGCACCGCCGAGGAAGTCGCCGCCGTCAAGACCTCCGACGAACTCGTC
AATGAACTCTTTGCCGCCGACACGATCGTCATCAGCACCGGCCTCATCAACTTCAATATCTATTCGTCGCTGAAGACCTG
GATCGACAACGTCGCCCGCGCCGGCCTGACGTTCAAGTACACGGAAAGCGGCCCGGTTGGCCTGCTCACCGGCAAGAAGG
TTTACGTGGTGCTCACCTCGGGCGGCGTCTACTCGCAGGGACCGGCTGCCCCCTTGAACCACGCCGTACCGTACCTGAAG
TCGGTTTTCGGCTTCCTCGGCATCACCGACATCGAGACCATCTACGTCGAAGGCCTCGCCTTCGGTCCTGAAGCTGCCGA
AAAGGCCATCGACGCTGCCAAGATGCGCGTCCAGGAAATTGCGCTGGCCGCTTAA

Upstream 100 bases:

>100_bases
CACCGTTTTTTCGATAGCAAGCCAGACCTATTTTCCGCTTCGAGACTGCGGCTCACCTCCCAAAACGGGCCGACAAATCC
AATTCGAAAGGAAATGCACC

Downstream 100 bases:

>100_bases
TCGGTTCTTCAACGCTTACCATAGTGTAGCGGCCGGCATTCGCCGGCCGTTGTCATTTGTCGATAGCAGCGACAAAATCC
CGGACCGTCTCGAGAATTTC

Product: NAD(P)H dehydrogenase (quinone)

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1 [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSSILLLTSSPRAESLSTPIAVELAEKLKNQNPGSFLVRRDLAASPLPHIDDLFTGAIRKPAEARTAEEVAAVKTSDELV
NELFAADTIVISTGLINFNIYSSLKTWIDNVARAGLTFKYTESGPVGLLTGKKVYVVLTSGGVYSQGPAAPLNHAVPYLK
SVFGFLGITDIETIYVEGLAFGPEAAEKAIDAAKMRVQEIALAA

Sequences:

>Translated_204_residues
MSSILLLTSSPRAESLSTPIAVELAEKLKNQNPGSFLVRRDLAASPLPHIDDLFTGAIRKPAEARTAEEVAAVKTSDELV
NELFAADTIVISTGLINFNIYSSLKTWIDNVARAGLTFKYTESGPVGLLTGKKVYVVLTSGGVYSQGPAAPLNHAVPYLK
SVFGFLGITDIETIYVEGLAFGPEAAEKAIDAAKMRVQEIALAA
>Mature_203_residues
SSILLLTSSPRAESLSTPIAVELAEKLKNQNPGSFLVRRDLAASPLPHIDDLFTGAIRKPAEARTAEEVAAVKTSDELVN
ELFAADTIVISTGLINFNIYSSLKTWIDNVARAGLTFKYTESGPVGLLTGKKVYVVLTSGGVYSQGPAAPLNHAVPYLKS
VFGFLGITDIETIYVEGLAFGPEAAEKAIDAAKMRVQEIALAA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=204, Percent_Identity=42.156862745098, Blast_Score=156, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 21680; Mature: 21549

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSILLLTSSPRAESLSTPIAVELAEKLKNQNPGSFLVRRDLAASPLPHIDDLFTGAIRK
CCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCC
PAEARTAEEVAAVKTSDELVNELFAADTIVISTGLINFNIYSSLKTWIDNVARAGLTFKY
CHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEECEEEEHHHHHHHHHHHHHHHCCCEEEE
TESGPVGLLTGKKVYVVLTSGGVYSQGPAAPLNHAVPYLKSVFGFLGITDIETIYVEGLA
CCCCCEEEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEECCC
FGPEAAEKAIDAAKMRVQEIALAA
CCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSILLLTSSPRAESLSTPIAVELAEKLKNQNPGSFLVRRDLAASPLPHIDDLFTGAIRK
CCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCC
PAEARTAEEVAAVKTSDELVNELFAADTIVISTGLINFNIYSSLKTWIDNVARAGLTFKY
CHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEECEEEEHHHHHHHHHHHHHHHCCCEEEE
TESGPVGLLTGKKVYVVLTSGGVYSQGPAAPLNHAVPYLKSVFGFLGITDIETIYVEGLA
CCCCCEEEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEECCC
FGPEAAEKAIDAAKMRVQEIALAA
CCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA