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The map label for this gene is maiA [H]

Identifier: 209549012

GI number: 209549012

Start: 1429784

End: 1430416

Strand: Direct

Name: maiA [H]

Synonym: Rleg2_1409

Alternate gene names: 209549012

Gene position: 1429784-1430416 (Clockwise)

Preceding gene: 209549011

Following gene: 209549015

Centisome position: 31.51

GC content: 60.66

Gene sequence:

>633_bases
ATGAGCGAGGTCGTTCTTTACGACTACTGGCGATCGTCGGCGAGCTATCGTGTCCGCATCGCTCTCAACCTGCTTGGCAT
CGACTACAGGACGGTGCCGGTCAACCTGCTTGAGGGAGCGCATCGGAAGCCGGACTATCTCACGCTCAACCCGCAAGGGC
TCGTGCCGACCCTGGTGATCGATGGAAAAACTCTCACCCAGTCCCTTGCCATCATCGAATATCTGGCCGAGCTTCGGCCG
GACCGCGGATTGCTGCCATCCGATAGCGCCGGCCGCCAGCATGTGCGCGCGCTGGCCTATGCGGTCGCCATGGACATCCA
TCCGATCTGCAATATGCATGTCGTGGCCCATCTCATGGCCGTGACGGAAAAGGAAGGTGTCCGCGAGGAATGGATGCAGT
ACTTCATCGCCGACGGGCTCCGCAAACTGGAGACGATGATAGGGGGACATGACGGCGCCTTCAGCTTTGGCGACGCGCCG
ACGATGGCAGACCTTTGCCTTATCCCGCAAGTTTACAACGCCCGCCGCTGGGGCGTCGATCTGAGCGATTTAAGGCGCAT
AGTGGATATCGATGCCCGGTGCGCCGAATTGCCGGCGTTTCAGGCAGCGCATCCTGACCGCGTGAAGCCGTGA

Upstream 100 bases:

>100_bases
TCGGCGATCAGGTCCGCATCGAGATGAAGGACCGTGCCGGCCATTCGATCTTTGGGGCGATCGAGCAGACCGTCGAACGT
TATGGATGAGAGGGGGCGCA

Downstream 100 bases:

>100_bases
CGTCGTCGTCGGGGGAGAGGCTCCTCCGGCAGCTGGACGGTTAGACTACTCGTCCCGCCACGAGTAAAATGTCGCAGCCT
GGCCCAACCGCTTGCCCGTT

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSEVVLYDYWRSSASYRVRIALNLLGIDYRTVPVNLLEGAHRKPDYLTLNPQGLVPTLVIDGKTLTQSLAIIEYLAELRP
DRGLLPSDSAGRQHVRALAYAVAMDIHPICNMHVVAHLMAVTEKEGVREEWMQYFIADGLRKLETMIGGHDGAFSFGDAP
TMADLCLIPQVYNARRWGVDLSDLRRIVDIDARCAELPAFQAAHPDRVKP

Sequences:

>Translated_210_residues
MSEVVLYDYWRSSASYRVRIALNLLGIDYRTVPVNLLEGAHRKPDYLTLNPQGLVPTLVIDGKTLTQSLAIIEYLAELRP
DRGLLPSDSAGRQHVRALAYAVAMDIHPICNMHVVAHLMAVTEKEGVREEWMQYFIADGLRKLETMIGGHDGAFSFGDAP
TMADLCLIPQVYNARRWGVDLSDLRRIVDIDARCAELPAFQAAHPDRVKP
>Mature_209_residues
SEVVLYDYWRSSASYRVRIALNLLGIDYRTVPVNLLEGAHRKPDYLTLNPQGLVPTLVIDGKTLTQSLAIIEYLAELRPD
RGLLPSDSAGRQHVRALAYAVAMDIHPICNMHVVAHLMAVTEKEGVREEWMQYFIADGLRKLETMIGGHDGAFSFGDAPT
MADLCLIPQVYNARRWGVDLSDLRRIVDIDARCAELPAFQAAHPDRVKP

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=205, Percent_Identity=46.8292682926829, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI22202622, Length=153, Percent_Identity=44.4444444444444, Blast_Score=121, Evalue=4e-28,
Organism=Homo sapiens, GI194440732, Length=205, Percent_Identity=37.0731707317073, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17551302, Length=204, Percent_Identity=44.1176470588235, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17510461, Length=208, Percent_Identity=39.4230769230769, Blast_Score=150, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17556142, Length=204, Percent_Identity=41.1764705882353, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45553325, Length=208, Percent_Identity=42.7884615384615, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24645375, Length=208, Percent_Identity=42.3076923076923, Blast_Score=166, Evalue=9e-42,
Organism=Drosophila melanogaster, GI21355859, Length=204, Percent_Identity=43.1372549019608, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21355857, Length=208, Percent_Identity=39.4230769230769, Blast_Score=153, Evalue=9e-38,
Organism=Drosophila melanogaster, GI17933730, Length=189, Percent_Identity=28.042328042328, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23525; Mature: 23394

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVVLYDYWRSSASYRVRIALNLLGIDYRTVPVNLLEGAHRKPDYLTLNPQGLVPTLVI
CCCEEEECCCCCCCCEEEEEEEEEECCCEEEECHHHHCCCCCCCCEEEECCCCCCEEEEE
DGKTLTQSLAIIEYLAELRPDRGLLPSDSAGRQHVRALAYAVAMDIHPICNMHVVAHLMA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VTEKEGVREEWMQYFIADGLRKLETMIGGHDGAFSFGDAPTMADLCLIPQVYNARRWGVD
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCC
LSDLRRIVDIDARCAELPAFQAAHPDRVKP
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEVVLYDYWRSSASYRVRIALNLLGIDYRTVPVNLLEGAHRKPDYLTLNPQGLVPTLVI
CCEEEECCCCCCCCEEEEEEEEEECCCEEEECHHHHCCCCCCCCEEEECCCCCCEEEEE
DGKTLTQSLAIIEYLAELRPDRGLLPSDSAGRQHVRALAYAVAMDIHPICNMHVVAHLMA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VTEKEGVREEWMQYFIADGLRKLETMIGGHDGAFSFGDAPTMADLCLIPQVYNARRWGVD
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCC
LSDLRRIVDIDARCAELPAFQAAHPDRVKP
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10220173; 11481430 [H]