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The map label for this gene is est [H]

Identifier: 209548973

GI number: 209548973

Start: 1388883

End: 1389926

Strand: Direct

Name: est [H]

Synonym: Rleg2_1370

Alternate gene names: 209548973

Gene position: 1388883-1389926 (Clockwise)

Preceding gene: 209548972

Following gene: 209548977

Centisome position: 30.61

GC content: 64.75

Gene sequence:

>1044_bases
ATGAACGGTCGGATTTTGCCAGCTGTCAAACGAAGCGCATCAAAGCGGCTCGGCGGTCTGTTGCCCGCATTCGCTGCGGT
GATGCTGCTGGCCTCGGCGGCAGCCGGAAAGGACATTGAACCGCAAAGCCAGGCCTCGCGCGATCAGGTCGAAAAACTCG
TCGGCCTCTGGAAGGATCATTTCACCGGCGCTGATGGCCTGCAGCAGCGGCGGACAGCCTTCCGGACGCTGATGGAAACG
ACGCCGGGGCCGACACGCATCCAGGTCCGGCAGGTCGATGCCGACGGCGTCGATGCCGAGCTGATGTGGCCCGCCCGTCT
TCATCATCCGATCGGCCAAAGGGTGATCCTCTATATTCATGGCGGCGGCTTTTCCGGCGGCTCCATCCGCACGCACAGCC
TGCTTGCCGGCTCGCTCGCCAAGGCCGCATCCAGCGACGTGCTTCTGATCGAGTATCGGCTGATGCCCGAATATACCTAT
CCCGCCCAGATCAATGACGCGCTGACGGCCTATCGCTGGCTTCTCGACAACGGCTATCGCGCCGAGAATGTCGTCGTGGC
GGGAGACGGCGCCGGCGGCAATATCGCGCTGGAGACGGTGCTGCGGCAGAGGCAGGCGGCAAAGCCGCTGCCGGCGGCGG
TCATTGCGCTCAGCCCCATCACCGATCTTGCCGCGACCGGCGGATCGATGACATCGAATGCCGACAGCGATCCGCTGGTC
GGCAGGGCGACGATCGAGACCTTGCGCAAGGCCTATCTCGGCAGCCGGTCGCCGAGCGATCCCCAGGCATCGCCGCTTTA
TGCCGACCTGACCGGCTTTCCGCCGCTGCTGCTGCAGGTCGGCTCCGGCGAAGTGCTGCTGGACGATACGCTGCGGCTCG
CCGACAAGGCGCGTCAGGCCGGGGTGGATGTCACCACCGAGATTTGGCCGGGCATGCCGCATCAGTGGCAGCTGTTTCCG
TCGCTGCTCGACGATGCCGACCGGTCGAGCCAGAATATCGCCGAATTTGCGATCCGGCATTTCGCAGACAAGCCGCAGGA
GTAG

Upstream 100 bases:

>100_bases
AAAGCTCAGGATTCGCATCCCTCACCCTTTTGACTCCAGCGCATGAACTGGCCAATGATGCTGCCATGGCCGCGATGGCC
AGCATCATCGGGAGAGGTTG

Downstream 100 bases:

>100_bases
GGCCGCGAGGCAGAGGCCTCACGTCCTCGCCGTCCGGATCATTGCGATGTGCCACGCGACGGTGACGGCGCCAGAGGCCG
CTCGACCTCCCGACCGACGC

Product: alpha/beta hydrolase fold-3 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNGRILPAVKRSASKRLGGLLPAFAAVMLLASAAAGKDIEPQSQASRDQVEKLVGLWKDHFTGADGLQQRRTAFRTLMET
TPGPTRIQVRQVDADGVDAELMWPARLHHPIGQRVILYIHGGGFSGGSIRTHSLLAGSLAKAASSDVLLIEYRLMPEYTY
PAQINDALTAYRWLLDNGYRAENVVVAGDGAGGNIALETVLRQRQAAKPLPAAVIALSPITDLAATGGSMTSNADSDPLV
GRATIETLRKAYLGSRSPSDPQASPLYADLTGFPPLLLQVGSGEVLLDDTLRLADKARQAGVDVTTEIWPGMPHQWQLFP
SLLDDADRSSQNIAEFAIRHFADKPQE

Sequences:

>Translated_347_residues
MNGRILPAVKRSASKRLGGLLPAFAAVMLLASAAAGKDIEPQSQASRDQVEKLVGLWKDHFTGADGLQQRRTAFRTLMET
TPGPTRIQVRQVDADGVDAELMWPARLHHPIGQRVILYIHGGGFSGGSIRTHSLLAGSLAKAASSDVLLIEYRLMPEYTY
PAQINDALTAYRWLLDNGYRAENVVVAGDGAGGNIALETVLRQRQAAKPLPAAVIALSPITDLAATGGSMTSNADSDPLV
GRATIETLRKAYLGSRSPSDPQASPLYADLTGFPPLLLQVGSGEVLLDDTLRLADKARQAGVDVTTEIWPGMPHQWQLFP
SLLDDADRSSQNIAEFAIRHFADKPQE
>Mature_347_residues
MNGRILPAVKRSASKRLGGLLPAFAAVMLLASAAAGKDIEPQSQASRDQVEKLVGLWKDHFTGADGLQQRRTAFRTLMET
TPGPTRIQVRQVDADGVDAELMWPARLHHPIGQRVILYIHGGGFSGGSIRTHSLLAGSLAKAASSDVLLIEYRLMPEYTY
PAQINDALTAYRWLLDNGYRAENVVVAGDGAGGNIALETVLRQRQAAKPLPAAVIALSPITDLAATGGSMTSNADSDPLV
GRATIETLRKAYLGSRSPSDPQASPLYADLTGFPPLLLQVGSGEVLLDDTLRLADKARQAGVDVTTEIWPGMPHQWQLFP
SLLDDADRSSQNIAEFAIRHFADKPQE

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=200, Percent_Identity=26.5, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI68051721, Length=120, Percent_Identity=32.5, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=126, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533410, Length=126, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=162, Percent_Identity=33.9506172839506, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71996133, Length=134, Percent_Identity=32.089552238806, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540028, Length=134, Percent_Identity=32.8358208955224, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24656084, Length=101, Percent_Identity=36.6336633663366, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24656076, Length=101, Percent_Identity=36.6336633663366, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20130169, Length=101, Percent_Identity=36.6336633663366, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 37304; Mature: 37304

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGRILPAVKRSASKRLGGLLPAFAAVMLLASAAAGKDIEPQSQASRDQVEKLVGLWKDH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
FTGADGLQQRRTAFRTLMETTPGPTRIQVRQVDADGVDAELMWPARLHHPIGQRVILYIH
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEECHHHHCCCCCCEEEEEEE
GGGFSGGSIRTHSLLAGSLAKAASSDVLLIEYRLMPEYTYPAQINDALTAYRWLLDNGYR
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCC
AENVVVAGDGAGGNIALETVLRQRQAAKPLPAAVIALSPITDLAATGGSMTSNADSDPLV
CCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCC
GRATIETLRKAYLGSRSPSDPQASPLYADLTGFPPLLLQVGSGEVLLDDTLRLADKARQA
HHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCEEEEECCCCEEEHHHHHHHHHHHHC
GVDVTTEIWPGMPHQWQLFPSLLDDADRSSQNIAEFAIRHFADKPQE
CCCEEHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNGRILPAVKRSASKRLGGLLPAFAAVMLLASAAAGKDIEPQSQASRDQVEKLVGLWKDH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
FTGADGLQQRRTAFRTLMETTPGPTRIQVRQVDADGVDAELMWPARLHHPIGQRVILYIH
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEECHHHHCCCCCCEEEEEEE
GGGFSGGSIRTHSLLAGSLAKAASSDVLLIEYRLMPEYTYPAQINDALTAYRWLLDNGYR
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCC
AENVVVAGDGAGGNIALETVLRQRQAAKPLPAAVIALSPITDLAATGGSMTSNADSDPLV
CCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCC
GRATIETLRKAYLGSRSPSDPQASPLYADLTGFPPLLLQVGSGEVLLDDTLRLADKARQA
HHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCEEEEECCCCEEEHHHHHHHHHHHHC
GVDVTTEIWPGMPHQWQLFPSLLDDADRSSQNIAEFAIRHFADKPQE
CCCEEHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]