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The map label for this gene is purR [H]

Identifier: 209548977

GI number: 209548977

Start: 1393669

End: 1394742

Strand: Direct

Name: purR [H]

Synonym: Rleg2_1374

Alternate gene names: 209548977

Gene position: 1393669-1394742 (Clockwise)

Preceding gene: 209548973

Following gene: 209548978

Centisome position: 30.71

GC content: 65.46

Gene sequence:

>1074_bases
ATGACAAAGGCGCGGGTAACCGTCATTGACATCGCAAAGGCCGCCGGCGTCTCCAAGTCGACGGTGTCGCTGGTGTTGCA
GGGCTCCTCCCTGGTCAATGAGGGGACGCGCTCCAAGGTCAATGCCGTGATGCGCGAACTGGGCTATGTCTATAATCGCG
GCGCCGCCAATCTTCGCCAGGCCGGTGCCAAATCGAAGATTATCGGCGTTGTCGTCAACGACCTGACGAACAGCTTTTTT
GCGGAGCTGGCGGTCGGCGTCGATATGGTCGTCCAGTCGGCCGGCTTCGTGCAGTTCCTGTCGAATACCGGCGAGAGCAT
CGACAGGCAGCGTGAGGTTGTCGCCTCGATGCGCGAGCACGGCATTTCCGGACTCATCGTCTCGCCGGCCCGCGCCACCG
ATGCCGCCGACTTCAAGCCGCTCGTTGCGGCAGGGATTCCAGTCGTGGTCGTCGTTCGAAACCTGCCCGGCGCCAAAGTC
TCGTCCATCGTTTCCGACAACCGGGCCGGCATGATGTCGGCCGTCAAGCATCTGGCCGGGCTCGGCCACAAGCGCATCGC
CTTTCTCGGCGGCTTTCCCGACACTGCCGTCTTCGACGACAGGCTTGCCGGTTATCGCAGCGGCGTGGAGGCGGCCGGGC
TCGATTATCATGAAGAACTCGTCATCTCCTCGGCGCCGTCGCGAGCCGGCGGGGTGGAGGCGATCGGAAAGGCGATCGCG
CTCGGCCGGCGGCCGACCGCAGCCGTCTGCTTCAACGATGCCGTGGCCTTCGGCGTTTGCGACGGGTTGCGCGCGCGCCG
CCTTGAGCCTGGCGCCGACTTCGCCGTGGTGGGCTTCGACGACGTCATCGAGGCGCAGGCGGCGGTTCCGGCGCTGACGA
CGGTTTCGGTCGACCCGCAGGGCATCGGGCGCCGCGGCGCGCAGATGCTGCTCAAGCAGATCAATGCCGGCAAGGCCGAG
GCGGAGACGGTGACGACAGCCGTTCGGTTGGTCGTTCGCGAAAGCTGCGGCGCCGGCAAGACCGCGCCGGCAAAGGCCGG
CAAGAGCGTAAAATCGAAACAGGACGCTGAGTAA

Upstream 100 bases:

>100_bases
AAAACGTTAGCATGAATGGCGCATGCGCAAATTTTCTCTTGACATAATTGGAACGTTCCAAATATTTATCCATCAGTCGA
CGTGATGGTCGGAGAGGGAA

Downstream 100 bases:

>100_bases
TGCACATGAAGAAGCATCTCACCCCAGCCGAAGCGGCCGCGCTCATCCCGGATGGCGTCGTCGTCACGGTATCGTCTTCC
AGCGGTCTCGGTTGCCCGGA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTKARVTVIDIAKAAGVSKSTVSLVLQGSSLVNEGTRSKVNAVMRELGYVYNRGAANLRQAGAKSKIIGVVVNDLTNSFF
AELAVGVDMVVQSAGFVQFLSNTGESIDRQREVVASMREHGISGLIVSPARATDAADFKPLVAAGIPVVVVVRNLPGAKV
SSIVSDNRAGMMSAVKHLAGLGHKRIAFLGGFPDTAVFDDRLAGYRSGVEAAGLDYHEELVISSAPSRAGGVEAIGKAIA
LGRRPTAAVCFNDAVAFGVCDGLRARRLEPGADFAVVGFDDVIEAQAAVPALTTVSVDPQGIGRRGAQMLLKQINAGKAE
AETVTTAVRLVVRESCGAGKTAPAKAGKSVKSKQDAE

Sequences:

>Translated_357_residues
MTKARVTVIDIAKAAGVSKSTVSLVLQGSSLVNEGTRSKVNAVMRELGYVYNRGAANLRQAGAKSKIIGVVVNDLTNSFF
AELAVGVDMVVQSAGFVQFLSNTGESIDRQREVVASMREHGISGLIVSPARATDAADFKPLVAAGIPVVVVVRNLPGAKV
SSIVSDNRAGMMSAVKHLAGLGHKRIAFLGGFPDTAVFDDRLAGYRSGVEAAGLDYHEELVISSAPSRAGGVEAIGKAIA
LGRRPTAAVCFNDAVAFGVCDGLRARRLEPGADFAVVGFDDVIEAQAAVPALTTVSVDPQGIGRRGAQMLLKQINAGKAE
AETVTTAVRLVVRESCGAGKTAPAKAGKSVKSKQDAE
>Mature_356_residues
TKARVTVIDIAKAAGVSKSTVSLVLQGSSLVNEGTRSKVNAVMRELGYVYNRGAANLRQAGAKSKIIGVVVNDLTNSFFA
ELAVGVDMVVQSAGFVQFLSNTGESIDRQREVVASMREHGISGLIVSPARATDAADFKPLVAAGIPVVVVVRNLPGAKVS
SIVSDNRAGMMSAVKHLAGLGHKRIAFLGGFPDTAVFDDRLAGYRSGVEAAGLDYHEELVISSAPSRAGGVEAIGKAIAL
GRRPTAAVCFNDAVAFGVCDGLRARRLEPGADFAVVGFDDVIEAQAAVPALTTVSVDPQGIGRRGAQMLLKQINAGKAEA
ETVTTAVRLVVRESCGAGKTAPAKAGKSVKSKQDAE

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787906, Length=293, Percent_Identity=32.4232081911263, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=30.7958477508651, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=28.2352941176471, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789068, Length=294, Percent_Identity=29.5918367346939, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=24.5454545454545, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=28.6144578313253, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1788474, Length=315, Percent_Identity=30.1587301587302, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=29.9684542586751, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786540, Length=357, Percent_Identity=29.6918767507003, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1790715, Length=335, Percent_Identity=26.865671641791, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787580, Length=334, Percent_Identity=25.4491017964072, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786268, Length=275, Percent_Identity=23.2727272727273, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790689, Length=326, Percent_Identity=25.4601226993865, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36927; Mature: 36796

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKARVTVIDIAKAAGVSKSTVSLVLQGSSLVNEGTRSKVNAVMRELGYVYNRGAANLRQ
CCCCEEEEEEHHHHCCCCHHEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
AGAKSKIIGVVVNDLTNSFFAELAVGVDMVVQSAGFVQFLSNTGESIDRQREVVASMREH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
GISGLIVSPARATDAADFKPLVAAGIPVVVVVRNLPGAKVSSIVSDNRAGMMSAVKHLAG
CCCEEEECCCCCCCCCCCCHHHHCCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHC
LGHKRIAFLGGFPDTAVFDDRLAGYRSGVEAAGLDYHEELVISSAPSRAGGVEAIGKAIA
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCCCHHHHHHHHH
LGRRPTAAVCFNDAVAFGVCDGLRARRLEPGADFAVVGFDDVIEAQAAVPALTTVSVDPQ
CCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHCCEEEEEECCCC
GIGRRGAQMLLKQINAGKAEAETVTTAVRLVVRESCGAGKTAPAKAGKSVKSKQDAE
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCC
>Mature Secondary Structure 
TKARVTVIDIAKAAGVSKSTVSLVLQGSSLVNEGTRSKVNAVMRELGYVYNRGAANLRQ
CCCEEEEEEHHHHCCCCHHEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
AGAKSKIIGVVVNDLTNSFFAELAVGVDMVVQSAGFVQFLSNTGESIDRQREVVASMREH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
GISGLIVSPARATDAADFKPLVAAGIPVVVVVRNLPGAKVSSIVSDNRAGMMSAVKHLAG
CCCEEEECCCCCCCCCCCCHHHHCCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHC
LGHKRIAFLGGFPDTAVFDDRLAGYRSGVEAAGLDYHEELVISSAPSRAGGVEAIGKAIA
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHEECCCCCCCCHHHHHHHHH
LGRRPTAAVCFNDAVAFGVCDGLRARRLEPGADFAVVGFDDVIEAQAAVPALTTVSVDPQ
CCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHCCEEEEEECCCC
GIGRRGAQMLLKQINAGKAEAETVTTAVRLVVRESCGAGKTAPAKAGKSVKSKQDAE
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA