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The map label for this gene is ycaN [H]

Identifier: 209548972

GI number: 209548972

Start: 1387894

End: 1388781

Strand: Direct

Name: ycaN [H]

Synonym: Rleg2_1369

Alternate gene names: 209548972

Gene position: 1387894-1388781 (Clockwise)

Preceding gene: 209548970

Following gene: 209548973

Centisome position: 30.58

GC content: 63.29

Gene sequence:

>888_bases
ATGCGGGCCACCGAACTGTCCGAACTCGCGGCTTTTGCGGCCGTTGCCCGGCATAGGAGTTTTCGCAAAGCCGGCGAGGA
GAGGGGTGTGACCGCCTCGGCGATCAGCCATGCGGTGCTCAACCTCGAAGACCGGATCGGCATTCGCCTGCTCAACCGCA
CGACGCGCAGCGTCTCGTTGACCGAGGCCGGCGAACTGCTGATCTCGCATCTCGACCCGGCCTTCGGCGAGATGGCGGCA
GCGCTCGATGCGCTGAACCGTTACCGAGACACGCCCTTCGGCAAGGTGAGGATCAACGTGCCGAATTCGATCGGCCCCTT
CGTCATCGGCCGCGTCATTGCCCCGCTGCTCCAACGCAATCCCAATCTGCAGCTGGAGATCAACGCGACCGACAGGCTGG
TCGATATCGTCGAGGAGGGCTTCGATGCCGGCATCCGTTTCGGCGAACGGGTGACCGAAGGCATGATCGCGCTGCGCATC
AAGCAGCGCATCCGCTTGGTCGTCGTCGGTTCGCCTGCCTATTTCGAGGTCCGGCCGAAGCCGGCGACGCCGCACGATCT
CAAGCGCCATCTTTGCATCCAGAACATGTTTCCGTCAGGCGCGCGTTATGCCTGGGAGTTCGAGAAGGATGGCCAGACGG
TCAGCTTTCAGCCGACAGGACCGCTGTCGCTCGATGATCACGAACTGATGCTGCAGGCAGCGCTCGGCGGCGTCGGCCTT
GCCTATATCTGGGAGCCGCGGGTGGAGAAGGCGATCGCCGACGGAGAACTGATCCAGGTGCTCCACGACTGGTGCCAGTC
GGAGGAGCCGCTCTATCTCTACTATCCGAGCCGACGCCACATGTCGGCGGGCTTCAGGGCGGTCATCGATGCGATGAAAG
CCGAGTAA

Upstream 100 bases:

>100_bases
CTCGGCGTCGCATCTCTGTGCTGACGGGTTGAATTTGACCGAAAATCCGTTGCTGTATAAGATTTGTTATTATTGAAACT
ATGATGAGCAATATTCAGTA

Downstream 100 bases:

>100_bases
AAAAGCTCAGGATTCGCATCCCTCACCCTTTTGACTCCAGCGCATGAACTGGCCAATGATGCTGCCATGGCCGCGATGGC
CAGCATCATCGGGAGAGGTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRATELSELAAFAAVARHRSFRKAGEERGVTASAISHAVLNLEDRIGIRLLNRTTRSVSLTEAGELLISHLDPAFGEMAA
ALDALNRYRDTPFGKVRINVPNSIGPFVIGRVIAPLLQRNPNLQLEINATDRLVDIVEEGFDAGIRFGERVTEGMIALRI
KQRIRLVVVGSPAYFEVRPKPATPHDLKRHLCIQNMFPSGARYAWEFEKDGQTVSFQPTGPLSLDDHELMLQAALGGVGL
AYIWEPRVEKAIADGELIQVLHDWCQSEEPLYLYYPSRRHMSAGFRAVIDAMKAE

Sequences:

>Translated_295_residues
MRATELSELAAFAAVARHRSFRKAGEERGVTASAISHAVLNLEDRIGIRLLNRTTRSVSLTEAGELLISHLDPAFGEMAA
ALDALNRYRDTPFGKVRINVPNSIGPFVIGRVIAPLLQRNPNLQLEINATDRLVDIVEEGFDAGIRFGERVTEGMIALRI
KQRIRLVVVGSPAYFEVRPKPATPHDLKRHLCIQNMFPSGARYAWEFEKDGQTVSFQPTGPLSLDDHELMLQAALGGVGL
AYIWEPRVEKAIADGELIQVLHDWCQSEEPLYLYYPSRRHMSAGFRAVIDAMKAE
>Mature_295_residues
MRATELSELAAFAAVARHRSFRKAGEERGVTASAISHAVLNLEDRIGIRLLNRTTRSVSLTEAGELLISHLDPAFGEMAA
ALDALNRYRDTPFGKVRINVPNSIGPFVIGRVIAPLLQRNPNLQLEINATDRLVDIVEEGFDAGIRFGERVTEGMIALRI
KQRIRLVVVGSPAYFEVRPKPATPHDLKRHLCIQNMFPSGARYAWEFEKDGQTVSFQPTGPLSLDDHELMLQAALGGVGL
AYIWEPRVEKAIADGELIQVLHDWCQSEEPLYLYYPSRRHMSAGFRAVIDAMKAE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=40.4844290657439, Blast_Score=230, Evalue=9e-62,
Organism=Escherichia coli, GI1787589, Length=294, Percent_Identity=36.3945578231293, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=33.5616438356164, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=30.2405498281787, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789440, Length=272, Percent_Identity=27.5735294117647, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI145693193, Length=280, Percent_Identity=30, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1789639, Length=290, Percent_Identity=26.2068965517241, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786448, Length=284, Percent_Identity=25.3521126760563, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789173, Length=279, Percent_Identity=27.5985663082437, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788748, Length=150, Percent_Identity=36.6666666666667, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=140, Percent_Identity=31.4285714285714, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790399, Length=123, Percent_Identity=29.2682926829268, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788706, Length=266, Percent_Identity=23.3082706766917, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786713, Length=278, Percent_Identity=25.8992805755396, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32867; Mature: 32867

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRATELSELAAFAAVARHRSFRKAGEERGVTASAISHAVLNLEDRIGIRLLNRTTRSVSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCCEEEH
TEAGELLISHLDPAFGEMAAALDALNRYRDTPFGKVRINVPNSIGPFVIGRVIAPLLQRN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCC
PNLQLEINATDRLVDIVEEGFDAGIRFGERVTEGMIALRIKQRIRLVVVGSPAYFEVRPK
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCEEEEECC
PATPHDLKRHLCIQNMFPSGARYAWEFEKDGQTVSFQPTGPLSLDDHELMLQAALGGVGL
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCE
AYIWEPRVEKAIADGELIQVLHDWCQSEEPLYLYYPSRRHMSAGFRAVIDAMKAE
EEEECHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRATELSELAAFAAVARHRSFRKAGEERGVTASAISHAVLNLEDRIGIRLLNRTTRSVSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCCEEEH
TEAGELLISHLDPAFGEMAAALDALNRYRDTPFGKVRINVPNSIGPFVIGRVIAPLLQRN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCC
PNLQLEINATDRLVDIVEEGFDAGIRFGERVTEGMIALRIKQRIRLVVVGSPAYFEVRPK
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCEEEEECC
PATPHDLKRHLCIQNMFPSGARYAWEFEKDGQTVSFQPTGPLSLDDHELMLQAALGGVGL
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCE
AYIWEPRVEKAIADGELIQVLHDWCQSEEPLYLYYPSRRHMSAGFRAVIDAMKAE
EEEECHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]