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The map label for this gene is amiC [H]

Identifier: 209548906

GI number: 209548906

Start: 1329265

End: 1330560

Strand: Direct

Name: amiC [H]

Synonym: Rleg2_1303

Alternate gene names: 209548906

Gene position: 1329265-1330560 (Clockwise)

Preceding gene: 209548903

Following gene: 209548907

Centisome position: 29.29

GC content: 63.27

Gene sequence:

>1296_bases
ATGCAGATAGGGTGTGGCGGTTTTTTGTTTAAGAGGGCTCGGATCGCGGCGAAATCCGCAGGGCGGCGATCGACATTCGC
AAGGCGGATTCTGACGGCGCTTCTGGCCGTCGGTCTGCTGTCGGCCGTTGCCGGTTCTGTCACCGCTGGAGATCCGCTGC
TTGCCTATGGCGCGCGCATCGTCGGCGACGATGCAAGGACCCGCATCGTCATCGATTTCGATCGCGAACCGCGCTTCTCG
GTGCACTATATCGCCAATCCGGAACGGATCGTCATCGATCTGCCGGCAACCGCCTTCGGTTTTCCGGCCAAGGATCTCGC
CGCCCGCGGCCTGTTCAAGGATATCCGTTACGGCAAGATGGACGAGGAAAGCGCCCGCATCGTCCTGACGACGGCAGGGC
CGGTGAAGCTGGCGCTCGCCAAGGTGCAGGCCGACGAGGCCGGCAAGGGGCATCGTCTCGTGCTCGATGCCGAGATGATC
GACAAGCAGGCCTTTTCGGAGCTGGTGAAAACGCAATCCTGGAGCGATCGAGCCTCGACCGATCAGGCCGATGCGGCCCA
GACGACGAGCGCCATTCCGGCACCTGAGAAAGCCGCCCCCGGCGATTTCGTCATCGCCGTCGACGCCGGTCATGGCGGCA
TCGATACCGGCGCGATCGGGGTCGATACCAAAACCGAGGAAAAGCAGGTGACGCTTGCCTTCGCCAAGGCCCTGACCGAG
CGGCTGAACAAGGAGCCGGGCATCAAGGCTTTCCTGACGCGCGAAGATGACGAGTTCCTGTCGCTGTCGCAGCGTGTGCT
GATCGCCCGCCAGAACCATGCCGGGTTGTTCATTTCGCTGCATGCCGACACGCTGAAGCAGAAGGATATCCGCGGCGCCA
CCGTCTACACCATCTCCGACAAGGCCTCCGACAAGCTCGCCGCCGACCTTGCCGAACGCGAAAACCTCTCCGACCAGATT
GCCGGCAAGGAGACGGTCGCCGAGCCGCCCGAGGTCGCCGATATCCTGCTCGATCTGACCCGGCGCGAGACCCAGGCCTT
CTCGATCTCGCTCGCCGAGAGCGTGCTGAATTCTTTCAAGGACCAGGTCGGCACCATCAACAATCCGCACCGTCATGCCG
GTTTCCGCGTGCTGCAGGCCCCCGACGTGCCGTCGATCCTCCTCGAGCTCGGCTTCCTCTCGAATGCCGACGATGAGAAA
CTGCTGCTCGACGAGACCTGGCGCGGCAAGATCGTCGGTCTTTTGACCGACGCAGTGAAGCGCTACCGCGCCGCCGTCAT
CGCGAATGGCGGCTGA

Upstream 100 bases:

>100_bases
TAACGCATAGAAACTGGGGATAAAGTGATTGCGATTCGGCAGGCCGCAAAGGGTGTTTCGTCCAGGCGACGTTCGACGCG
GCCGCGTCGGCTCCATCGCA

Downstream 100 bases:

>100_bases
TGCCTGCCAACCCCATAAATCCATAGGGGGTGCGGGTCGGCGAATCCATGAAAACAAAGACTTAATGTGCATCGCATGAC
TCAAAGTCTGAGCAGGATGG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQIGCGGFLFKRARIAAKSAGRRSTFARRILTALLAVGLLSAVAGSVTAGDPLLAYGARIVGDDARTRIVIDFDREPRFS
VHYIANPERIVIDLPATAFGFPAKDLAARGLFKDIRYGKMDEESARIVLTTAGPVKLALAKVQADEAGKGHRLVLDAEMI
DKQAFSELVKTQSWSDRASTDQADAAQTTSAIPAPEKAAPGDFVIAVDAGHGGIDTGAIGVDTKTEEKQVTLAFAKALTE
RLNKEPGIKAFLTREDDEFLSLSQRVLIARQNHAGLFISLHADTLKQKDIRGATVYTISDKASDKLAADLAERENLSDQI
AGKETVAEPPEVADILLDLTRRETQAFSISLAESVLNSFKDQVGTINNPHRHAGFRVLQAPDVPSILLELGFLSNADDEK
LLLDETWRGKIVGLLTDAVKRYRAAVIANGG

Sequences:

>Translated_431_residues
MQIGCGGFLFKRARIAAKSAGRRSTFARRILTALLAVGLLSAVAGSVTAGDPLLAYGARIVGDDARTRIVIDFDREPRFS
VHYIANPERIVIDLPATAFGFPAKDLAARGLFKDIRYGKMDEESARIVLTTAGPVKLALAKVQADEAGKGHRLVLDAEMI
DKQAFSELVKTQSWSDRASTDQADAAQTTSAIPAPEKAAPGDFVIAVDAGHGGIDTGAIGVDTKTEEKQVTLAFAKALTE
RLNKEPGIKAFLTREDDEFLSLSQRVLIARQNHAGLFISLHADTLKQKDIRGATVYTISDKASDKLAADLAERENLSDQI
AGKETVAEPPEVADILLDLTRRETQAFSISLAESVLNSFKDQVGTINNPHRHAGFRVLQAPDVPSILLELGFLSNADDEK
LLLDETWRGKIVGLLTDAVKRYRAAVIANGG
>Mature_431_residues
MQIGCGGFLFKRARIAAKSAGRRSTFARRILTALLAVGLLSAVAGSVTAGDPLLAYGARIVGDDARTRIVIDFDREPRFS
VHYIANPERIVIDLPATAFGFPAKDLAARGLFKDIRYGKMDEESARIVLTTAGPVKLALAKVQADEAGKGHRLVLDAEMI
DKQAFSELVKTQSWSDRASTDQADAAQTTSAIPAPEKAAPGDFVIAVDAGHGGIDTGAIGVDTKTEEKQVTLAFAKALTE
RLNKEPGIKAFLTREDDEFLSLSQRVLIARQNHAGLFISLHADTLKQKDIRGATVYTISDKASDKLAADLAERENLSDQI
AGKETVAEPPEVADILLDLTRRETQAFSISLAESVLNSFKDQVGTINNPHRHAGFRVLQAPDVPSILLELGFLSNADDEK
LLLDETWRGKIVGLLTDAVKRYRAAVIANGG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=413, Percent_Identity=32.4455205811138, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788776, Length=238, Percent_Identity=37.3949579831933, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790611, Length=411, Percent_Identity=27.7372262773723, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46408; Mature: 46408

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGCGGFLFKRARIAAKSAGRRSTFARRILTALLAVGLLSAVAGSVTAGDPLLAYGARI
CEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEE
VGDDARTRIVIDFDREPRFSVHYIANPERIVIDLPATAFGFPAKDLAARGLFKDIRYGKM
ECCCCCEEEEEEECCCCCEEEEEECCCCEEEEECCCHHCCCCHHHHHHHHHHHHHCCCCC
DEESARIVLTTAGPVKLALAKVQADEAGKGHRLVLDAEMIDKQAFSELVKTQSWSDRAST
CCCCCEEEEEECCCCEEEEEEHCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCC
DQADAAQTTSAIPAPEKAAPGDFVIAVDAGHGGIDTGAIGVDTKTEEKQVTLAFAKALTE
CCCHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHH
RLNKEPGIKAFLTREDDEFLSLSQRVLIARQNHAGLFISLHADTLKQKDIRGATVYTISD
HHCCCCCCEEEEECCCHHHHHHHHHEEEEECCCCCEEEEEECHHHHHCCCCCCEEEEECC
KASDKLAADLAERENLSDQIAGKETVAEPPEVADILLDLTRRETQAFSISLAESVLNSFK
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQVGTINNPHRHAGFRVLQAPDVPSILLELGFLSNADDEKLLLDETWRGKIVGLLTDAVK
HHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHH
RYRAAVIANGG
HHHHHEEECCC
>Mature Secondary Structure
MQIGCGGFLFKRARIAAKSAGRRSTFARRILTALLAVGLLSAVAGSVTAGDPLLAYGARI
CEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEE
VGDDARTRIVIDFDREPRFSVHYIANPERIVIDLPATAFGFPAKDLAARGLFKDIRYGKM
ECCCCCEEEEEEECCCCCEEEEEECCCCEEEEECCCHHCCCCHHHHHHHHHHHHHCCCCC
DEESARIVLTTAGPVKLALAKVQADEAGKGHRLVLDAEMIDKQAFSELVKTQSWSDRAST
CCCCCEEEEEECCCCEEEEEEHCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCC
DQADAAQTTSAIPAPEKAAPGDFVIAVDAGHGGIDTGAIGVDTKTEEKQVTLAFAKALTE
CCCHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHH
RLNKEPGIKAFLTREDDEFLSLSQRVLIARQNHAGLFISLHADTLKQKDIRGATVYTISD
HHCCCCCCEEEEECCCHHHHHHHHHEEEEECCCCCEEEEEECHHHHHCCCCCCEEEEECC
KASDKLAADLAERENLSDQIAGKETVAEPPEVADILLDLTRRETQAFSISLAESVLNSFK
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQVGTINNPHRHAGFRVLQAPDVPSILLELGFLSNADDEKLLLDETWRGKIVGLLTDAVK
HHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHH
RYRAAVIANGG
HHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]