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The map label for this gene is pyrB [H]

Identifier: 209548902

GI number: 209548902

Start: 1322333

End: 1323289

Strand: Reverse

Name: pyrB [H]

Synonym: Rleg2_1299

Alternate gene names: 209548902

Gene position: 1323289-1322333 (Counterclockwise)

Preceding gene: 209548904

Following gene: 209548901

Centisome position: 29.16

GC content: 64.79

Gene sequence:

>957_bases
ATGGTCTTTTTCCCCCACCGCCACCTCATCGGCATCAAGGGCCTCACCGAGCAGGATATCACCTATCTGCTCGACAAGGC
CGACGAGGCCGTCAAGATCAGCCGCCAGCGAGAGAAGAAGACGTCGACGCTGCGCGGGCTGACGCAGATCAACCTCTTTT
TCGAGGCATCGACCCGCACGCAGGCGTCGTTCGAGCTTGCCGGCAAGCGCCTCGGCGCCGACGTCATGAACATGTCGGTC
GGCAATTCCTCGGTGAAGAAGGGCGAGACGCTGATCGATACGGCGATGACGCTGAATGCGATGCGCCCCGACGTGCTGGT
CATCCGCCATTCGAGCGCGGGGGCGGCCGCCCTGCTTGCCCAGAAGGTCTCCTGCTCGGTCGTCAATGCCGGCGACGGGC
AGCATGAACATCCGACCCAGGCGCTGCTCGATGCACTGACGATCCGCCGCGCCAAGGGCAAGCTCTCGCGCATCATCGTG
GCGATCTGCGGCGACGTGCTACATTCGCGGGTGGCGCGCTCCAATATCCTGCTGCTGAACGCCATGGGCGCGCGCGTGCG
CGTCGTCGCCCCTGCCACTCTTCTTCCCGCCGGCATCGCCGATATGGGCGTCGAGGTCTTCCATTCGATGAAGGAAGGGC
TGAAGGACGCCGATGTGGTGATGATGCTGCGGCTGCAGCGCGAGCGCATGTCCGGCGCCTTCGTGCCTTCGGTGCGCGAA
TACTACCACTTCTACGGGCTCGACGCCGAAACGCTGAAGGCGGCGAAGGAGGATGCGCTGGTCATGCATCCGGGCCCGAT
GAACCGCGGCGTCGAGATCGCCTCCGAGGTGGCGGACGGTCCGCAGAGCGTGATCGCCGAACAGGTGGAAATGGGGGTCG
CGGTGCGCATGGCGGTGATGGAGACGCTGCTCGTCTCGCAGAACCAGGGCCCCCGAACCGATGGATTGAAGGCATGA

Upstream 100 bases:

>100_bases
GCGAAAATGCATGGGGACATAAGCCTTTGCCCACAGCGCATGCTTGCCCCAGCGGGCAGCACTCTTTATAGACCCGCCAG
AGACAGCAAGAGGCAGGGTC

Downstream 100 bases:

>100_bases
GCAACCCGATCGTCCTCCAGAACGTCCGCATCGTCGATCCCTCGCGCAATCTCGACGAGGTGGGCACGATCATCGCCGCA
AACGGCGTGATCCTTGCTGC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MVFFPHRHLIGIKGLTEQDITYLLDKADEAVKISRQREKKTSTLRGLTQINLFFEASTRTQASFELAGKRLGADVMNMSV
GNSSVKKGETLIDTAMTLNAMRPDVLVIRHSSAGAAALLAQKVSCSVVNAGDGQHEHPTQALLDALTIRRAKGKLSRIIV
AICGDVLHSRVARSNILLLNAMGARVRVVAPATLLPAGIADMGVEVFHSMKEGLKDADVVMMLRLQRERMSGAFVPSVRE
YYHFYGLDAETLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIAEQVEMGVAVRMAVMETLLVSQNQGPRTDGLKA

Sequences:

>Translated_318_residues
MVFFPHRHLIGIKGLTEQDITYLLDKADEAVKISRQREKKTSTLRGLTQINLFFEASTRTQASFELAGKRLGADVMNMSV
GNSSVKKGETLIDTAMTLNAMRPDVLVIRHSSAGAAALLAQKVSCSVVNAGDGQHEHPTQALLDALTIRRAKGKLSRIIV
AICGDVLHSRVARSNILLLNAMGARVRVVAPATLLPAGIADMGVEVFHSMKEGLKDADVVMMLRLQRERMSGAFVPSVRE
YYHFYGLDAETLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIAEQVEMGVAVRMAVMETLLVSQNQGPRTDGLKA
>Mature_318_residues
MVFFPHRHLIGIKGLTEQDITYLLDKADEAVKISRQREKKTSTLRGLTQINLFFEASTRTQASFELAGKRLGADVMNMSV
GNSSVKKGETLIDTAMTLNAMRPDVLVIRHSSAGAAALLAQKVSCSVVNAGDGQHEHPTQALLDALTIRRAKGKLSRIIV
AICGDVLHSRVARSNILLLNAMGARVRVVAPATLLPAGIADMGVEVFHSMKEGLKDADVVMMLRLQRERMSGAFVPSVRE
YYHFYGLDAETLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIAEQVEMGVAVRMAVMETLLVSQNQGPRTDGLKA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=37.012987012987, Blast_Score=183, Evalue=2e-46,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=34.9514563106796, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790703, Length=201, Percent_Identity=31.8407960199005, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=325, Percent_Identity=34.7692307692308, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=35.7142857142857, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24642586, Length=275, Percent_Identity=36.7272727272727, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34440; Mature: 34440

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFFPHRHLIGIKGLTEQDITYLLDKADEAVKISRQREKKTSTLRGLTQINLFFEASTRT
CEECCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCC
QASFELAGKRLGADVMNMSVGNSSVKKGETLIDTAMTLNAMRPDVLVIRHSSAGAAALLA
HHHHHHHHHHHCCHHHHCCCCCCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
QKVSCSVVNAGDGQHEHPTQALLDALTIRRAKGKLSRIIVAICGDVLHSRVARSNILLLN
HHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
AMGARVRVVAPATLLPAGIADMGVEVFHSMKEGLKDADVVMMLRLQRERMSGAFVPSVRE
ECCCEEEEEECHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHH
YYHFYGLDAETLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIAEQVEMGVAVRMAVM
HHHHHCCCHHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ETLLVSQNQGPRTDGLKA
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MVFFPHRHLIGIKGLTEQDITYLLDKADEAVKISRQREKKTSTLRGLTQINLFFEASTRT
CEECCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCC
QASFELAGKRLGADVMNMSVGNSSVKKGETLIDTAMTLNAMRPDVLVIRHSSAGAAALLA
HHHHHHHHHHHCCHHHHCCCCCCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
QKVSCSVVNAGDGQHEHPTQALLDALTIRRAKGKLSRIIVAICGDVLHSRVARSNILLLN
HHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
AMGARVRVVAPATLLPAGIADMGVEVFHSMKEGLKDADVVMMLRLQRERMSGAFVPSVRE
ECCCEEEEEECHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHH
YYHFYGLDAETLKAAKEDALVMHPGPMNRGVEIASEVADGPQSVIAEQVEMGVAVRMAVM
HHHHHCCCHHHHHHHHCCCEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ETLLVSQNQGPRTDGLKA
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA