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The map label for this gene is topA [H]

Identifier: 209548898

GI number: 209548898

Start: 1316414

End: 1319068

Strand: Reverse

Name: topA [H]

Synonym: Rleg2_1295

Alternate gene names: 209548898

Gene position: 1319068-1316414 (Counterclockwise)

Preceding gene: 209548899

Following gene: 209548897

Centisome position: 29.07

GC content: 62.79

Gene sequence:

>2655_bases
ATGAATGTTGTAGTGGTGGAATCGCCTGCCAAGGCCAAGACGATCAACAAGTATCTGGGTTCGGGATACAAAGTGCTCGC
CTCTTTCGGCCATGTGCGCGATCTGCCTGCCAAGGATGGCTCGGTCCTCCCCGATCAGGATTTCGAAATGCTTTGGGAGG
TCGACGGGGCCTCGGCCAAGCGGATGAAGGACATCGCCGACGCGGTGAAATCGTCCGACGGCCTGTTTCTCGCAACCGAC
CCGGATCGCGAAGGCGAAGCGATTTCCTGGCACGTCCTCGATATGTTGAACAAGAAGCGGGTGCTGAACGGCAAGCCGGT
CAAGCGTGTCGTCTTCAACGCGATCACCAAGAAGGCGGTGCTCGACGCGATGGCCGATCCGCGCGACATCGACGTGCCGC
TGGTCGACGCCTATCTGGCGCGCCGCGCGCTCGACTATCTCGTCGGCTTCAATCTTTCGCCGGTCCTCTGGCGCAAGCTG
CCGGGCGCACGCTCGGCCGGCCGCGTCCAGTCGGTGGCGCTTCGCTTGGTCTGCGACCGCGAATCCGAAATCGAGCGCTT
CATTTCGGAAGAATACTGGAACATCTCAGCGCTTCTGAAGACGCCGCGCGGCGACGAGTTCGAGGCAAGGCTGGTTTCGG
CGAACGGCAAGCGGCTGCAGCCGCGCGCGATCGGCAACGGCGAGGATGCGGGCCGCCTGAAGGCATTGCTGGACGGCGCG
AGCTATGTCGTCGACACGGTCGAGGCCAAGCCGGTCAAGCGCAATCCGGGACCGCCATTTACGACCTCGACCCTGCAGCA
GGCCGCCTCCTCCAATCTTGGCTTCTCCGCCTCGCGCACCATGCAGATAGCCCAGAAGCTTTATGAAGGCGTCGATATCG
GCGGCGAGACCGTCGGTCTCATCACTTATATGCGAACCGACGGCGTGCAGATGGCGCCGGAGGCGATCGATGCCGCCCGC
AGCGCCATCGTCGACCAGTTCGGCGAGCGTTACATGCCCGAAAAGGCGCGCTTCTATTCCACCAAGGCCAAGAACGCCCA
GGAGGCGCACGAGGCGATCCGCCCGACCGATTTCTACCGCTCGCCTGACCGTGTGCGCCAATTCCTCGATGCCGACCAGA
TCCGGCTCTACGACCTGATCTGGAAGCGCGGCATCGCCAGCCAGATGGCCTCGGCCGAAATCGAGCGCACGACGGCTGAA
ATCACCGCCGACAACAAGGGCGAGAAGGCCGGGCTGCGCGCCGTCGGCTCGGTCATTCGCTTCGATGGCTTCATTGCCGC
CTACACCGATCAGAAGGAAGATGGCGAGCAGAGCGACGACGGCGACGAGGATGGCCGCCTGCCGGAGATCAATGCGCGCG
AGACGCTCGCCAAGCAGAAGATCAATTCGACCCAGCATTTTACCGAGCCGCCGCCGCGCTACTCGGAAGCCTCGCTGATC
AAGAAGATGGAAGAGCTCGGCATCGGCCGCCCCTCCACCTATGCCGCGACGCTGGCCACACTGCGCGACCGCGACTATGT
GACGATCGACAAGCGCAAGCTGATCCCGCAGGCCAAGGGCCGGCTTGTGACGGCTTTCCTCGAAAGCTTCTTCACCAAAT
ATGTCGAATACGACTTCACCGCCGATCTCGAGGAAAAGCTCGACCGGATTTCCGCCGGCGAGCTGAACTGGAAGCAGGTG
CTGCGCGATTTCTGGAAGGATTTCTTCGCCCAGATCGAAGACACCAAGGAACTGCGCGTCACCAATGTGCTCGATTCGCT
GAACGAGGCGCTGGCGCCGCTGGTCTTCCCGAAGCGGGAGGACGGCAGCGATCCCCGGATCTGCCAGGTCTGCGGCACCG
GCAACCTGTCGCTGAAGCTCGGCAAATATGGCGCCTTTGTCGGCTGCTCGAACTATCCGGAATGCAATTACACCCGCCAG
CTCTCCTCCGAAAACGGCGGAGAAGCGGATGGTGCTGCCATCAACGAACCGAAGAACCTCGGCACCGATCCGACGACCGG
CGAGGAACTGACGCTGCGATCCGGCCGCTTCGGCCCCTATATCCAGCGCGGCGACGGCAAGGAGGCCAAACGCGCTTCGC
TGCCGAAGGGCTGGAAGCCTGAGGACATCGACTACGAAAAGGCCATGGCGCTGATTTCGCTGCCGCGCGATATCGGCAAA
CATCCGGAATCCGGCAAGATGATCTCATCAGGCATCGGCCGCTATGGGCCGTTCCTGCTGCATGACGGTTCCTATGCCAA
TCTGGAAACGATCGAGGACGTGTTTTCGGTCGGGCTCAACCGCGCCGTGACCGTCATTGCCGAAAAGGCAAACCAGGCAC
CGGGCCGTGGTTCGCGCGGCACGCCGGCGGCGCTGAAGACGCTCGGCGATCATCCGGACGGCGGCGCAATCACCGTTCGC
GACGGCAAGTACGGGCCTTATGTCAACTGGGGCAAGGTCAATGCCACCCTGCCGAAGGGCAAGGATCCGCAGGCGATCAC
CGTCGAAGAGGCGCTTGCGCTGATCACGGAGAAGGCCGGTAAGGCGCCCGCCGGCAAGACGAGCAAGGCGAAAGCCAAGC
CGAAGGCGGCCGCCGCCGAAGCCAAGACCACCAAGACGGCGGCCAAGCCGAAGGCAACGAAGGCGAAAGCGCCCGCGAAA
TCGAAAAAGAGCTGA

Upstream 100 bases:

>100_bases
CAGCGATTTCCCTGTCCATGTCGGAGGGCCGGCATCCATGTTGCGGTGTGTGTTGCGCTGGTTTCAACGCTGGCGTCGTT
TCCTTTTTTAGAGAATCATC

Downstream 100 bases:

>100_bases
CGTGAGCAGAATACCGCGCGACAGAACGAACCCTGCGGGCAAGCGCCCGGGCAAGATCGGCCGTGCCGGCAAGGATGCTC
CCGCCGTCGAGCCGCTGAAC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 884; Mature: 884

Protein sequence:

>884_residues
MNVVVVESPAKAKTINKYLGSGYKVLASFGHVRDLPAKDGSVLPDQDFEMLWEVDGASAKRMKDIADAVKSSDGLFLATD
PDREGEAISWHVLDMLNKKRVLNGKPVKRVVFNAITKKAVLDAMADPRDIDVPLVDAYLARRALDYLVGFNLSPVLWRKL
PGARSAGRVQSVALRLVCDRESEIERFISEEYWNISALLKTPRGDEFEARLVSANGKRLQPRAIGNGEDAGRLKALLDGA
SYVVDTVEAKPVKRNPGPPFTTSTLQQAASSNLGFSASRTMQIAQKLYEGVDIGGETVGLITYMRTDGVQMAPEAIDAAR
SAIVDQFGERYMPEKARFYSTKAKNAQEAHEAIRPTDFYRSPDRVRQFLDADQIRLYDLIWKRGIASQMASAEIERTTAE
ITADNKGEKAGLRAVGSVIRFDGFIAAYTDQKEDGEQSDDGDEDGRLPEINARETLAKQKINSTQHFTEPPPRYSEASLI
KKMEELGIGRPSTYAATLATLRDRDYVTIDKRKLIPQAKGRLVTAFLESFFTKYVEYDFTADLEEKLDRISAGELNWKQV
LRDFWKDFFAQIEDTKELRVTNVLDSLNEALAPLVFPKREDGSDPRICQVCGTGNLSLKLGKYGAFVGCSNYPECNYTRQ
LSSENGGEADGAAINEPKNLGTDPTTGEELTLRSGRFGPYIQRGDGKEAKRASLPKGWKPEDIDYEKAMALISLPRDIGK
HPESGKMISSGIGRYGPFLLHDGSYANLETIEDVFSVGLNRAVTVIAEKANQAPGRGSRGTPAALKTLGDHPDGGAITVR
DGKYGPYVNWGKVNATLPKGKDPQAITVEEALALITEKAGKAPAGKTSKAKAKPKAAAAEAKTTKTAAKPKATKAKAPAK
SKKS

Sequences:

>Translated_884_residues
MNVVVVESPAKAKTINKYLGSGYKVLASFGHVRDLPAKDGSVLPDQDFEMLWEVDGASAKRMKDIADAVKSSDGLFLATD
PDREGEAISWHVLDMLNKKRVLNGKPVKRVVFNAITKKAVLDAMADPRDIDVPLVDAYLARRALDYLVGFNLSPVLWRKL
PGARSAGRVQSVALRLVCDRESEIERFISEEYWNISALLKTPRGDEFEARLVSANGKRLQPRAIGNGEDAGRLKALLDGA
SYVVDTVEAKPVKRNPGPPFTTSTLQQAASSNLGFSASRTMQIAQKLYEGVDIGGETVGLITYMRTDGVQMAPEAIDAAR
SAIVDQFGERYMPEKARFYSTKAKNAQEAHEAIRPTDFYRSPDRVRQFLDADQIRLYDLIWKRGIASQMASAEIERTTAE
ITADNKGEKAGLRAVGSVIRFDGFIAAYTDQKEDGEQSDDGDEDGRLPEINARETLAKQKINSTQHFTEPPPRYSEASLI
KKMEELGIGRPSTYAATLATLRDRDYVTIDKRKLIPQAKGRLVTAFLESFFTKYVEYDFTADLEEKLDRISAGELNWKQV
LRDFWKDFFAQIEDTKELRVTNVLDSLNEALAPLVFPKREDGSDPRICQVCGTGNLSLKLGKYGAFVGCSNYPECNYTRQ
LSSENGGEADGAAINEPKNLGTDPTTGEELTLRSGRFGPYIQRGDGKEAKRASLPKGWKPEDIDYEKAMALISLPRDIGK
HPESGKMISSGIGRYGPFLLHDGSYANLETIEDVFSVGLNRAVTVIAEKANQAPGRGSRGTPAALKTLGDHPDGGAITVR
DGKYGPYVNWGKVNATLPKGKDPQAITVEEALALITEKAGKAPAGKTSKAKAKPKAAAAEAKTTKTAAKPKATKAKAPAK
SKKS
>Mature_884_residues
MNVVVVESPAKAKTINKYLGSGYKVLASFGHVRDLPAKDGSVLPDQDFEMLWEVDGASAKRMKDIADAVKSSDGLFLATD
PDREGEAISWHVLDMLNKKRVLNGKPVKRVVFNAITKKAVLDAMADPRDIDVPLVDAYLARRALDYLVGFNLSPVLWRKL
PGARSAGRVQSVALRLVCDRESEIERFISEEYWNISALLKTPRGDEFEARLVSANGKRLQPRAIGNGEDAGRLKALLDGA
SYVVDTVEAKPVKRNPGPPFTTSTLQQAASSNLGFSASRTMQIAQKLYEGVDIGGETVGLITYMRTDGVQMAPEAIDAAR
SAIVDQFGERYMPEKARFYSTKAKNAQEAHEAIRPTDFYRSPDRVRQFLDADQIRLYDLIWKRGIASQMASAEIERTTAE
ITADNKGEKAGLRAVGSVIRFDGFIAAYTDQKEDGEQSDDGDEDGRLPEINARETLAKQKINSTQHFTEPPPRYSEASLI
KKMEELGIGRPSTYAATLATLRDRDYVTIDKRKLIPQAKGRLVTAFLESFFTKYVEYDFTADLEEKLDRISAGELNWKQV
LRDFWKDFFAQIEDTKELRVTNVLDSLNEALAPLVFPKREDGSDPRICQVCGTGNLSLKLGKYGAFVGCSNYPECNYTRQ
LSSENGGEADGAAINEPKNLGTDPTTGEELTLRSGRFGPYIQRGDGKEAKRASLPKGWKPEDIDYEKAMALISLPRDIGK
HPESGKMISSGIGRYGPFLLHDGSYANLETIEDVFSVGLNRAVTVIAEKANQAPGRGSRGTPAALKTLGDHPDGGAITVR
DGKYGPYVNWGKVNATLPKGKDPQAITVEEALALITEKAGKAPAGKTSKAKAKPKAAAAEAKTTKTAAKPKATKAKAPAK
SKKS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=606, Percent_Identity=26.4026402640264, Blast_Score=110, Evalue=6e-24,
Organism=Escherichia coli, GI1787529, Length=686, Percent_Identity=38.0466472303207, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1788061, Length=654, Percent_Identity=24.0061162079511, Blast_Score=116, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=584, Percent_Identity=25.6849315068493, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17555378, Length=519, Percent_Identity=24.8554913294798, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585251, Length=658, Percent_Identity=25.8358662613982, Blast_Score=137, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 96730; Mature: 96730

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVVVESPAKAKTINKYLGSGYKVLASFGHVRDLPAKDGSVLPDQDFEMLWEVDGASAK
CCEEEECCCHHHHHHHHHHCCCHHHHHHHCCHHCCCCCCCCCCCCCCHHHHEECCCCHHH
RMKDIADAVKSSDGLFLATDPDREGEAISWHVLDMLNKKRVLNGKPVKRVVFNAITKKAV
HHHHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LDAMADPRDIDVPLVDAYLARRALDYLVGFNLSPVLWRKLPGARSAGRVQSVALRLVCDR
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCC
ESEIERFISEEYWNISALLKTPRGDEFEARLVSANGKRLQPRAIGNGEDAGRLKALLDGA
HHHHHHHHHHHHCCEEHEEECCCCCCCEEEEEECCCCEECCCCCCCCCCHHHHHHHHCCH
SYVVDTVEAKPVKRNPGPPFTTSTLQQAASSNLGFSASRTMQIAQKLYEGVDIGGETVGL
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
ITYMRTDGVQMAPEAIDAARSAIVDQFGERYMPEKARFYSTKAKNAQEAHEAIRPTDFYR
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHCCCHHHCC
SPDRVRQFLDADQIRLYDLIWKRGIASQMASAEIERTTAEITADNKGEKAGLRAVGSVIR
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
FDGFIAAYTDQKEDGEQSDDGDEDGRLPEINARETLAKQKINSTQHFTEPPPRYSEASLI
HCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
KKMEELGIGRPSTYAATLATLRDRDYVTIDKRKLIPQAKGRLVTAFLESFFTKYVEYDFT
HHHHHHCCCCCCHHHHHHHHHCCCCEEEEHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCC
ADLEEKLDRISAGELNWKQVLRDFWKDFFAQIEDTKELRVTNVLDSLNEALAPLVFPKRE
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
DGSDPRICQVCGTGNLSLKLGKYGAFVGCSNYPECNYTRQLSSENGGEADGAAINEPKNL
CCCCCCEEEEECCCCEEEEECCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCC
GTDPTTGEELTLRSGRFGPYIQRGDGKEAKRASLPKGWKPEDIDYEKAMALISLPRDIGK
CCCCCCCCEEEEECCCCCCCEECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHCC
HPESGKMISSGIGRYGPFLLHDGSYANLETIEDVFSVGLNRAVTVIAEKANQAPGRGSRG
CCCCCCHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCC
TPAALKTLGDHPDGGAITVRDGKYGPYVNWGKVNATLPKGKDPQAITVEEALALITEKAG
CHHHHHHCCCCCCCCEEEEECCCCCCCEECCCEEEECCCCCCCCEEEHHHHHHHHHHHCC
KAPAGKTSKAKAKPKAAAAEAKTTKTAAKPKATKAKAPAKSKKS
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNVVVVESPAKAKTINKYLGSGYKVLASFGHVRDLPAKDGSVLPDQDFEMLWEVDGASAK
CCEEEECCCHHHHHHHHHHCCCHHHHHHHCCHHCCCCCCCCCCCCCCHHHHEECCCCHHH
RMKDIADAVKSSDGLFLATDPDREGEAISWHVLDMLNKKRVLNGKPVKRVVFNAITKKAV
HHHHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LDAMADPRDIDVPLVDAYLARRALDYLVGFNLSPVLWRKLPGARSAGRVQSVALRLVCDR
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCC
ESEIERFISEEYWNISALLKTPRGDEFEARLVSANGKRLQPRAIGNGEDAGRLKALLDGA
HHHHHHHHHHHHCCEEHEEECCCCCCCEEEEEECCCCEECCCCCCCCCCHHHHHHHHCCH
SYVVDTVEAKPVKRNPGPPFTTSTLQQAASSNLGFSASRTMQIAQKLYEGVDIGGETVGL
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
ITYMRTDGVQMAPEAIDAARSAIVDQFGERYMPEKARFYSTKAKNAQEAHEAIRPTDFYR
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHCCCHHHCC
SPDRVRQFLDADQIRLYDLIWKRGIASQMASAEIERTTAEITADNKGEKAGLRAVGSVIR
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
FDGFIAAYTDQKEDGEQSDDGDEDGRLPEINARETLAKQKINSTQHFTEPPPRYSEASLI
HCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
KKMEELGIGRPSTYAATLATLRDRDYVTIDKRKLIPQAKGRLVTAFLESFFTKYVEYDFT
HHHHHHCCCCCCHHHHHHHHHCCCCEEEEHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCC
ADLEEKLDRISAGELNWKQVLRDFWKDFFAQIEDTKELRVTNVLDSLNEALAPLVFPKRE
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
DGSDPRICQVCGTGNLSLKLGKYGAFVGCSNYPECNYTRQLSSENGGEADGAAINEPKNL
CCCCCCEEEEECCCCEEEEECCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCC
GTDPTTGEELTLRSGRFGPYIQRGDGKEAKRASLPKGWKPEDIDYEKAMALISLPRDIGK
CCCCCCCCEEEEECCCCCCCEECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHCC
HPESGKMISSGIGRYGPFLLHDGSYANLETIEDVFSVGLNRAVTVIAEKANQAPGRGSRG
CCCCCCHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCC
TPAALKTLGDHPDGGAITVRDGKYGPYVNWGKVNATLPKGKDPQAITVEEALALITEKAG
CHHHHHHCCCCCCCCEEEEECCCCCCCEECCCEEEECCCCCCCCEEEHHHHHHHHHHHCC
KAPAGKTSKAKAKPKAAAAEAKTTKTAAKPKATKAKAPAKSKKS
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA