Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 209548893

GI number: 209548893

Start: 1309698

End: 1311071

Strand: Direct

Name: pleD [H]

Synonym: Rleg2_1290

Alternate gene names: 209548893

Gene position: 1309698-1311071 (Clockwise)

Preceding gene: 209548892

Following gene: 209548903

Centisome position: 28.86

GC content: 63.17

Gene sequence:

>1374_bases
ATGACTGCGCGAATACTGGTGGTTGACGATATTCCGGCCAATGTGAAGCTGCTCGAAGCGCGGCTGCTCGCGGAGTATTT
CGACGTGATGACCGCAGCCGACGGCTATACGGCGCTGGCGATCTGCGAGCGCAACCAGGTCGATCTGATCCTGCTCGACA
TCATGATGCCCGGCATAGACGGTTTCGAGGTCTGCGAGCGGCTGAAGGCCAACCGCAAGACCGCCCATATTCCTGTCGTC
ATGGTTACGGCACTGGATCAGCCGGCCGATCGCGTCCGCGGCCTGAAGGCCGGCGCCGACGATTTCCTCACCAAACCGGT
CAACGATCTTCAGCTGATCTCACGGGTGAAGAGCCTGCTGCGGCTGAAGACGTTGAGCGATGAGTTGCGCATCCGCGCGG
ACACCGCCCATACGATGGGCATCGACGATCTCATGCGGGCAGGCGAGGGCCGCGCCGACGAGACCGGTCAGATCCTGCTC
GTCGACGGCCGCGCCAATTCGCAGGAGCGCATCATTAAGGCGCTGAAGCCCGTCGCCGACGTGCTTGCCCTTTCCGATCC
GCAGGCAGCGCTCTTCGAGGCGGCCGAAAGCGCGTTCGATCTCGTCATCGTCAACGCCAATTTCGACGATTACGATCCGC
TGCGCCTCTGCTCGCAGCTGCGCTCGCTGGAGCGCACCCGCTTTCTGCCGATCCTGATCATCACTGAGCAGGGCGCGGAC
GAGATGGTCGTGCGCGCGCTCGATCTCGGCGTCAACGATTACATCATCCGCCCGGTCGATCCCAACGAGCTGGTTGCCCG
CAGCCTGACGCAGATCCGTCGCAAGCGCTGCAACGACCGCCTGCGCGCCAGCGTCAAGCAGACGATCGAGCTTGCCGTGA
CCGATCCGCTGACCGGCCTTTATAACCGGCGTTATCTCGACAATCACCTGAACGTGCTTTTCAACCGTTCGATGGCGCGG
GGCCGGCCGCTTTCGGTGCTGATCACCGATATCGATCGCTTCAAGCAGGTAAACGACACCTATGGCCATGACGGCGGCGA
CGAAGTGCTGCGGGAGTTTGCCAACCGCGTCCGCTCGACCATTCGCGGCGCCGACCTCGCTTGCCGCTACGGCGGGGAGG
AATTCGTCGTCGTCATGCCCGATACCTCGCCGGAAATTGCCGCCGCTGTTGCCGAGCGCCTGCGCGCGGCCGTCGAAAAC
GCGCCGTTCTTGCTGAAACATGCGGGAGAGGCGCTGAATGTCACCGCTTCCTTCGGCATCGCTTCGCGCATCGGCTCGGT
GCTGACCCCGGATCAGCTGATGAAACAGGCCGATCTTGCGCTTTACGAGGCCAAGAATACCGGCCGCAATCGCGTCGTCG
CCGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
CGGCTGCGAAGCCTATGTTTCCAAGCCGATCTCGGTTCCGAAATTCATCGAGACGATCAAGACCTATCTGGGCGATGCCT
GACGCATCGGAAAGACAGCT

Downstream 100 bases:

>100_bases
CGAATCACCTGTCGCCTTATGTGCCGTTTATGGGTTTTCCACCGTGGTAAGCGGATTGTTACCGATTTTTAATGCTCGCC
TGCACAGCGCGGCGTGAAAA

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTARILVVDDIPANVKLLEARLLAEYFDVMTAADGYTALAICERNQVDLILLDIMMPGIDGFEVCERLKANRKTAHIPVV
MVTALDQPADRVRGLKAGADDFLTKPVNDLQLISRVKSLLRLKTLSDELRIRADTAHTMGIDDLMRAGEGRADETGQILL
VDGRANSQERIIKALKPVADVLALSDPQAALFEAAESAFDLVIVNANFDDYDPLRLCSQLRSLERTRFLPILIITEQGAD
EMVVRALDLGVNDYIIRPVDPNELVARSLTQIRRKRCNDRLRASVKQTIELAVTDPLTGLYNRRYLDNHLNVLFNRSMAR
GRPLSVLITDIDRFKQVNDTYGHDGGDEVLREFANRVRSTIRGADLACRYGGEEFVVVMPDTSPEIAAAVAERLRAAVEN
APFLLKHAGEALNVTASFGIASRIGSVLTPDQLMKQADLALYEAKNTGRNRVVAAAA

Sequences:

>Translated_457_residues
MTARILVVDDIPANVKLLEARLLAEYFDVMTAADGYTALAICERNQVDLILLDIMMPGIDGFEVCERLKANRKTAHIPVV
MVTALDQPADRVRGLKAGADDFLTKPVNDLQLISRVKSLLRLKTLSDELRIRADTAHTMGIDDLMRAGEGRADETGQILL
VDGRANSQERIIKALKPVADVLALSDPQAALFEAAESAFDLVIVNANFDDYDPLRLCSQLRSLERTRFLPILIITEQGAD
EMVVRALDLGVNDYIIRPVDPNELVARSLTQIRRKRCNDRLRASVKQTIELAVTDPLTGLYNRRYLDNHLNVLFNRSMAR
GRPLSVLITDIDRFKQVNDTYGHDGGDEVLREFANRVRSTIRGADLACRYGGEEFVVVMPDTSPEIAAAVAERLRAAVEN
APFLLKHAGEALNVTASFGIASRIGSVLTPDQLMKQADLALYEAKNTGRNRVVAAAA
>Mature_456_residues
TARILVVDDIPANVKLLEARLLAEYFDVMTAADGYTALAICERNQVDLILLDIMMPGIDGFEVCERLKANRKTAHIPVVM
VTALDQPADRVRGLKAGADDFLTKPVNDLQLISRVKSLLRLKTLSDELRIRADTAHTMGIDDLMRAGEGRADETGQILLV
DGRANSQERIIKALKPVADVLALSDPQAALFEAAESAFDLVIVNANFDDYDPLRLCSQLRSLERTRFLPILIITEQGADE
MVVRALDLGVNDYIIRPVDPNELVARSLTQIRRKRCNDRLRASVKQTIELAVTDPLTGLYNRRYLDNHLNVLFNRSMARG
RPLSVLITDIDRFKQVNDTYGHDGGDEVLREFANRVRSTIRGADLACRYGGEEFVVVMPDTSPEIAAAVAERLRAAVENA
PFLLKHAGEALNVTASFGIASRIGSVLTPDQLMKQADLALYEAKNTGRNRVVAAAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=206, Percent_Identity=38.8349514563107, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI145693134, Length=165, Percent_Identity=43.6363636363636, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=40.8536585365854, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=38.5093167701863, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=35.672514619883, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=40.4761904761905, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=36.2573099415205, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=39.1304347826087, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788956, Length=179, Percent_Identity=36.3128491620112, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=34.5238095238095, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788394, Length=158, Percent_Identity=36.0759493670886, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081974, Length=111, Percent_Identity=36.036036036036, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=38.135593220339, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=40.495867768595, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI2367329, Length=161, Percent_Identity=37.2670807453416, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=34.7457627118644, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081977, Length=261, Percent_Identity=27.5862068965517, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787541, Length=183, Percent_Identity=31.6939890710383, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790863, Length=133, Percent_Identity=33.8345864661654, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=34.7457627118644, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=38.8349514563107, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787229, Length=120, Percent_Identity=35, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789149, Length=108, Percent_Identity=35.1851851851852, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790860, Length=117, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788550, Length=134, Percent_Identity=32.089552238806, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081921, Length=172, Percent_Identity=29.6511627906977, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50471; Mature: 50340

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARILVVDDIPANVKLLEARLLAEYFDVMTAADGYTALAICERNQVDLILLDIMMPGID
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEHHCCCCC
GFEVCERLKANRKTAHIPVVMVTALDQPADRVRGLKAGADDFLTKPVNDLQLISRVKSLL
HHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKTLSDELRIRADTAHTMGIDDLMRAGEGRADETGQILLVDGRANSQERIIKALKPVAD
HHHHCCHHHEEEECCCHHCCHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
VLALSDPQAALFEAAESAFDLVIVNANFDDYDPLRLCSQLRSLERTRFLPILIITEQGAD
HHHCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCH
EMVVRALDLGVNDYIIRPVDPNELVARSLTQIRRKRCNDRLRASVKQTIELAVTDPLTGL
HHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHH
YNRRYLDNHLNVLFNRSMARGRPLSVLITDIDRFKQVNDTYGHDGGDEVLREFANRVRST
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
IRGADLACRYGGEEFVVVMPDTSPEIAAAVAERLRAAVENAPFLLKHAGEALNVTASFGI
HCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEECCCH
ASRIGSVLTPDQLMKQADLALYEAKNTGRNRVVAAAA
HHHHCCCCCHHHHHHHHHHHHEECCCCCCCCEEEECC
>Mature Secondary Structure 
TARILVVDDIPANVKLLEARLLAEYFDVMTAADGYTALAICERNQVDLILLDIMMPGID
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEHHCCCCC
GFEVCERLKANRKTAHIPVVMVTALDQPADRVRGLKAGADDFLTKPVNDLQLISRVKSLL
HHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHH
RLKTLSDELRIRADTAHTMGIDDLMRAGEGRADETGQILLVDGRANSQERIIKALKPVAD
HHHHCCHHHEEEECCCHHCCHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
VLALSDPQAALFEAAESAFDLVIVNANFDDYDPLRLCSQLRSLERTRFLPILIITEQGAD
HHHCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCH
EMVVRALDLGVNDYIIRPVDPNELVARSLTQIRRKRCNDRLRASVKQTIELAVTDPLTGL
HHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHH
YNRRYLDNHLNVLFNRSMARGRPLSVLITDIDRFKQVNDTYGHDGGDEVLREFANRVRST
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
IRGADLACRYGGEEFVVVMPDTSPEIAAAVAERLRAAVENAPFLLKHAGEALNVTASFGI
HCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEECCCH
ASRIGSVLTPDQLMKQADLALYEAKNTGRNRVVAAAA
HHHHCCCCCHHHHHHHHHHHHEECCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA