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The map label for this gene is nuoB1 [H]

Identifier: 209548863

GI number: 209548863

Start: 1277203

End: 1277787

Strand: Direct

Name: nuoB1 [H]

Synonym: Rleg2_1260

Alternate gene names: 209548863

Gene position: 1277203-1277787 (Clockwise)

Preceding gene: 209548862

Following gene: 209548864

Centisome position: 28.14

GC content: 63.08

Gene sequence:

>585_bases
ATGGGAGTAGCCCCTGTGAGCAATCAGCCGCTCGTCGCCCAGCAGCCGAAGGGGATCATCGATCCCTCCACAGGCAAGCC
GATCGGCAGCAATGACGCGTTTTTCGGCGAGATCAACAATGAGCTCGCCGACAAGGGTTTCCTCGTCACCTCGACCGACG
AGCTGATCAACTGGGCTCGTACCGGCTCGCTGATGTGGATGACCTTCGGTCTCGCCTGCTGCGCCGTCGAAATGATGCAG
CTGTCGATGCCGCGTTACGACGTCGAGCGTTTCGGCTTTGCGCCGCGCGCCTCGCCGCGCCAGTCCGACGTCATGATCGT
CGCCGGCACGCTGACCAACAAGATGGCGCCCGCGCTCCGCAAGGTCTACGACCAGATGCCCGAGCCGCGCTACGTCATCT
CGATGGGTTCCTGCGCCAATGGCGGCGGTTACTATCATTATTCCTACTCGGTGGTGCGCGGCTGCGACCGCATCGTGCCG
ATCGACATCTACGTGCCGGGCTGTCCCCCCACGGCAGAGGCGCTGCTTTACGGCGTGCTTCTGCTGCAGAAGAAGATCCG
GCGCACCGGCACGATCGAACGCTAA

Upstream 100 bases:

>100_bases
CCTTCGGCGCCATCGGCTGGTTCGGCTTCTGGTCCATGATGGTCTTCCTCTTCGTGCTGACCATCGGCTTTATCTATGAA
TGGAAAAAGGGAGCCCTGGA

Downstream 100 bases:

>100_bases
GGGTTAAGGACAAGGCATATGAGTGAAGCCCTGACTGAGCTTGCGTCCTACCTTGGCGAAGCGCGCGGCAATCTGATCGC
CGCATCGCAGATGAAGTATG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 1; NDH-1 subunit B 1 [H]

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MGVAPVSNQPLVAQQPKGIIDPSTGKPIGSNDAFFGEINNELADKGFLVTSTDELINWARTGSLMWMTFGLACCAVEMMQ
LSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVP
IDIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER

Sequences:

>Translated_194_residues
MGVAPVSNQPLVAQQPKGIIDPSTGKPIGSNDAFFGEINNELADKGFLVTSTDELINWARTGSLMWMTFGLACCAVEMMQ
LSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVP
IDIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER
>Mature_193_residues
GVAPVSNQPLVAQQPKGIIDPSTGKPIGSNDAFFGEINNELADKGFLVTSTDELINWARTGSLMWMTFGLACCAVEMMQL
SMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPI
DIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=78.2312925170068, Blast_Score=252, Evalue=1e-67,
Organism=Escherichia coli, GI1788624, Length=168, Percent_Identity=45.2380952380952, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=42.8571428571429, Blast_Score=106, Evalue=9e-25,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=42.2764227642276, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=176, Percent_Identity=60.7954545454545, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI18859983, Length=159, Percent_Identity=71.0691823899371, Blast_Score=249, Evalue=9e-67,
Organism=Drosophila melanogaster, GI24642371, Length=159, Percent_Identity=71.0691823899371, Blast_Score=249, Evalue=9e-67,
Organism=Drosophila melanogaster, GI24651058, Length=192, Percent_Identity=61.4583333333333, Blast_Score=245, Evalue=1e-65,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 21278; Mature: 21147

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVAPVSNQPLVAQQPKGIIDPSTGKPIGSNDAFFGEINNELADKGFLVTSTDELINWAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHCHHHHCCCEEEECHHHHHHHHH
TGSLMWMTFGLACCAVEMMQLSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALR
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHH
KVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPIDIYVPGCPPTAEALLYGVL
HHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHH
LLQKKIRRTGTIER
HHHHHHHHCCCCCC
>Mature Secondary Structure 
GVAPVSNQPLVAQQPKGIIDPSTGKPIGSNDAFFGEINNELADKGFLVTSTDELINWAR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHCHHHHCCCEEEECHHHHHHHHH
TGSLMWMTFGLACCAVEMMQLSMPRYDVERFGFAPRASPRQSDVMIVAGTLTNKMAPALR
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHH
KVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPIDIYVPGCPPTAEALLYGVL
HHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHH
LLQKKIRRTGTIER
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA