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The map label for this gene is cmlA [H]

Identifier: 209548835

GI number: 209548835

Start: 1245396

End: 1246586

Strand: Reverse

Name: cmlA [H]

Synonym: Rleg2_1232

Alternate gene names: 209548835

Gene position: 1246586-1245396 (Counterclockwise)

Preceding gene: 209548837

Following gene: 209548834

Centisome position: 27.47

GC content: 61.29

Gene sequence:

>1191_bases
ATGTCTTATCCGAAAATTCCGTCCTGGACGTATTCCGTGCCGGCGACGTTGTTGCTGATGGCGCCTTTCGACATCCTGGC
CTCGCTTGCAATGGACATCTATCTGCCGGTTGTGCCGACCATGCCGCAGGCGCTTGGCACCTCGCCTGCCGTCATTCAAT
TGACCCTTAGCCTTTACATGCTTGTGCTTGGCGTCGGGCAGATCGTCTTTGGACCGATCTCCGATATCGTTGGGCGGCGG
CCCGTTCTGCTCGGCGGGGCGGCACTCTTTGCCGTTTCCTCCTTCCTACTTGCCGGAACCTCATCGGCCTCTCTCTTCAT
GGCCCTGCGATTGCTGCAGGCCATCGGCGCCTCGGCGGCGCTGGTCGCCACTTTCGCAACGGTGCGTGACGTTTATGCCG
AACGCCCGGAAAGCAGCGTGATCTATAGCCTGCTTGGTTCCATGCTGTCCTTTGTGCCCGCATTCGGGCCGATCGTTGGA
GCGGTGATCGCGGATCGGTACGGGTGGCGAGCCATCTTCGTCATCATCGGCGTGCTTTCGGTCGTCGCAATTCTCAACGC
CGGCATGCGGTGGCATGAAACCCGCCCTGCCGCCGCCACGGCGACCATCTCGTTCCGGCCGATTCTGACCAGCTTTCCGT
TCTGGGTCTACACAAGCGGCTTCAGCGCGGCGATGGGAGCCTTCTTCGTCTTCTTCTCGACGGCACCTCGCATCCTGATC
GATCAATCCGGTTTTTCCGGCATCACCTTCAGCTTCATCTTTGCCTCGGTGGCTTTGGTGATGATCGTGACGGCGCGCTT
TGCCAAAGGCTTCGTCACGCGCTGGGGTATCGCAGGCAGCCTTGCCAGAGGCATGGCGATGTTGCTTCTCGGCGCCGTGA
TGCTTGCCGCGGGAGAGATGTTTCTGCAGGCACCCCTCATCGGCTTCGTCCTGCCGATGTGGATCATCGCGATCGGCATC
GTCTTTGCGACGGCGGTCACCGCCAATGGTGCACTCAAGGCGTTCGACGATACCGCCGGAACGGCGGTGGCGCTCTATTT
CTGCATCGAAAGCCTGCTGGTCGGGGTCGCAGGCACCTTGTTCGTCGTATTGCTCGACGGCGACACCGCCTGGCCGCTGG
CAGCTTATGCCGCCGTGACGGCGCTGATCACCCTTGCCGGATTGCGGCGGCTTTCGTGCAAGACAGCCTGA

Upstream 100 bases:

>100_bases
AACAACTCTGCCCTGTTGTTGCGGCGTCTGTCCGGCCAATCCCGATCCTGACGAAAAGACAGATCGCCGCCGAATGAAAT
CATTTGAGCGGATCTTACCC

Downstream 100 bases:

>100_bases
CGAACTTGCTGCGGAAAGGCGGCAGCTCGAAAACCAGGAGCGGCCGTCAAGCGAAGGATCGCTTGAAATCTCGCCCCACC
TCGCCTACCCCTTTCCTGCC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSYPKIPSWTYSVPATLLLMAPFDILASLAMDIYLPVVPTMPQALGTSPAVIQLTLSLYMLVLGVGQIVFGPISDIVGRR
PVLLGGAALFAVSSFLLAGTSSASLFMALRLLQAIGASAALVATFATVRDVYAERPESSVIYSLLGSMLSFVPAFGPIVG
AVIADRYGWRAIFVIIGVLSVVAILNAGMRWHETRPAAATATISFRPILTSFPFWVYTSGFSAAMGAFFVFFSTAPRILI
DQSGFSGITFSFIFASVALVMIVTARFAKGFVTRWGIAGSLARGMAMLLLGAVMLAAGEMFLQAPLIGFVLPMWIIAIGI
VFATAVTANGALKAFDDTAGTAVALYFCIESLLVGVAGTLFVVLLDGDTAWPLAAYAAVTALITLAGLRRLSCKTA

Sequences:

>Translated_396_residues
MSYPKIPSWTYSVPATLLLMAPFDILASLAMDIYLPVVPTMPQALGTSPAVIQLTLSLYMLVLGVGQIVFGPISDIVGRR
PVLLGGAALFAVSSFLLAGTSSASLFMALRLLQAIGASAALVATFATVRDVYAERPESSVIYSLLGSMLSFVPAFGPIVG
AVIADRYGWRAIFVIIGVLSVVAILNAGMRWHETRPAAATATISFRPILTSFPFWVYTSGFSAAMGAFFVFFSTAPRILI
DQSGFSGITFSFIFASVALVMIVTARFAKGFVTRWGIAGSLARGMAMLLLGAVMLAAGEMFLQAPLIGFVLPMWIIAIGI
VFATAVTANGALKAFDDTAGTAVALYFCIESLLVGVAGTLFVVLLDGDTAWPLAAYAAVTALITLAGLRRLSCKTA
>Mature_395_residues
SYPKIPSWTYSVPATLLLMAPFDILASLAMDIYLPVVPTMPQALGTSPAVIQLTLSLYMLVLGVGQIVFGPISDIVGRRP
VLLGGAALFAVSSFLLAGTSSASLFMALRLLQAIGASAALVATFATVRDVYAERPESSVIYSLLGSMLSFVPAFGPIVGA
VIADRYGWRAIFVIIGVLSVVAILNAGMRWHETRPAAATATISFRPILTSFPFWVYTSGFSAAMGAFFVFFSTAPRILID
QSGFSGITFSFIFASVALVMIVTARFAKGFVTRWGIAGSLARGMAMLLLGAVMLAAGEMFLQAPLIGFVLPMWIIAIGIV
FATAVTANGALKAFDDTAGTAVALYFCIESLLVGVAGTLFVVLLDGDTAWPLAAYAAVTALITLAGLRRLSCKTA

Specific function: Appears to provoke a reduction of the content of the major porins ompA and ompC [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=228, Percent_Identity=27.6315789473684, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788509, Length=380, Percent_Identity=26.0526315789474, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI48994889, Length=337, Percent_Identity=24.3323442136499, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI87082312, Length=198, Percent_Identity=31.8181818181818, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790794, Length=259, Percent_Identity=24.3243243243243, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87081738, Length=160, Percent_Identity=33.75, Blast_Score=65, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=147, Percent_Identity=29.2517006802721, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322071, Length=175, Percent_Identity=25.7142857142857, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41619; Mature: 41488

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYPKIPSWTYSVPATLLLMAPFDILASLAMDIYLPVVPTMPQALGTSPAVIQLTLSLYM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHH
LVLGVGQIVFGPISDIVGRRPVLLGGAALFAVSSFLLAGTSSASLFMALRLLQAIGASAA
HHHHHHHHHHHCHHHHCCCCCEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHH
LVATFATVRDVYAERPESSVIYSLLGSMLSFVPAFGPIVGAVIADRYGWRAIFVIIGVLS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VVAILNAGMRWHETRPAAATATISFRPILTSFPFWVYTSGFSAAMGAFFVFFSTAPRILI
HHHHHHCCCCCCCCCCCHHEEEEEECHHHHHCCHHHEECCHHHHHHHHHHHHHCCCCEEE
DQSGFSGITFSFIFASVALVMIVTARFAKGFVTRWGIAGSLARGMAMLLLGAVMLAAGEM
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
FLQAPLIGFVLPMWIIAIGIVFATAVTANGALKAFDDTAGTAVALYFCIESLLVGVAGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
FVVLLDGDTAWPLAAYAAVTALITLAGLRRLSCKTA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYPKIPSWTYSVPATLLLMAPFDILASLAMDIYLPVVPTMPQALGTSPAVIQLTLSLYM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHH
LVLGVGQIVFGPISDIVGRRPVLLGGAALFAVSSFLLAGTSSASLFMALRLLQAIGASAA
HHHHHHHHHHHCHHHHCCCCCEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHH
LVATFATVRDVYAERPESSVIYSLLGSMLSFVPAFGPIVGAVIADRYGWRAIFVIIGVLS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VVAILNAGMRWHETRPAAATATISFRPILTSFPFWVYTSGFSAAMGAFFVFFSTAPRILI
HHHHHHCCCCCCCCCCCHHEEEEEECHHHHHCCHHHEECCHHHHHHHHHHHHHCCCCEEE
DQSGFSGITFSFIFASVALVMIVTARFAKGFVTRWGIAGSLARGMAMLLLGAVMLAAGEM
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
FLQAPLIGFVLPMWIIAIGIVFATAVTANGALKAFDDTAGTAVALYFCIESLLVGVAGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
FVVLLDGDTAWPLAAYAAVTALITLAGLRRLSCKTA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1648560; 1658833 [H]