Click here to switch to the map view.

The map label for this gene is glyA [H]

Identifier: 209548780

GI number: 209548780

Start: 1188557

End: 1189855

Strand: Direct

Name: glyA [H]

Synonym: Rleg2_1177

Alternate gene names: 209548780

Gene position: 1188557-1189855 (Clockwise)

Preceding gene: 209548779

Following gene: 209548781

Centisome position: 26.19

GC content: 62.28

Gene sequence:

>1299_bases
ATGACCAATGCTTCCACCGAGTCCTTCTTCAATCGCTCGCTTGCGGACGTCGATCCCGAGATTTTCGGCGCGATCGGAAA
GGAACTCGGTCGCCAACGGCACGAGATCGAACTGATCGCTTCCGAGAACATCGTCTCCCGCGCCGTGCTGGAAGCTCAGG
GCTCCATCATGACCAACAAATATGCCGAGGGTTATCCCGGCAAGCGTTATTACGGCGGCTGCCAGTTCGTCGATATCGCC
GAGGAGCTGGCGATCGAGCGCGCCAAGAAGCTGTTCGGCGTCAATTTCGCCAACGTCCAGCCGAATTCGGGCTCGCAGAT
GAACCAGGCCGTGTTCCTGGCGCTGCTGCAGCCGGGCGACACCTTCATGGGTCTCGACCTGAATTCGGGCGGCCACCTCA
CGCATGGTTCGCCGGTCAACATGTCCGGCAAGTGGTTCAACGTCGTCTCCTACGGCGTGCGCGAAGGCGACAACCTGCTC
GACATGGATGAAGTCGAGCGCAAAGCCAAGGAAACCAAGCCGAAGCTGATCATCGCCGGCGGCACCGCCTATTCCCGCAT
CTGGGACTGGAAGCGCTTCCGCGAGATCGCCGATTCTGTCGGTGCCTATCTGATGGTCGACATGGCCCACATCGCCGGCC
TCGTCGCCGGCGGCCAGCATCCGTCGCCGTTCCCGCATTGCCATGTCGCGACGACGACGACCCACAAGTCGCTGCGCGGC
CCGCGTGGCGGCGTCATCCTCACCAATGAAGAGGATCTGGCGAAGAAGTTCAATTCGGCCGTTTTCCCCGGCCTGCAGGG
TGGTCCGCTGATGCACATCATCGCCGCCAAAGCCGTCGCTTTCGGCGAGGCGCTGCAGCCCGAGTTCAAGGATTACGCCG
CCCAGGTCGTCAAGAACGCCAAGGCGCTGGCCGAAACGCTGATCGCTGGTGGTCTCGACGTCGTCTCCGGTGGCACCGAC
AACCATCTGATGCTGGTCGACCTGCGCAAGAAGAACGCCACCGGCAAACGCGCCGAAGCTGCTCTCGGCCGCGCCTACGT
CACCTGCAACAAGAACGGCATTCCCTTCGATCCGGAGAAGCCCTTCGTCACCTCCGGTGTGCGCCTCGGCGCGCCGGCCG
GCACCACCCGCGGCTTCAAGGAAGCCGAATTCCGCGAAATCGGCAATCTCATCGTCGAGGTTCTCGACGGCCTGAAGGCT
GCCAATTCCGATGAAGGCAACGCCGCCGTCGAAGCCGCCGTGCGCGGCAAGGTGGTCAACCTCACTGACCGCTTCCCAAT
GTATGACTACATGGGATAA

Upstream 100 bases:

>100_bases
GACGGGAGCGCGCGCTTTGCTCAGCAAATGTCGTTCTCCGTATTGCCCTTGCCACGGCGGAAGGCTAATACCTCAGCGCA
TTCCAGTTGAGGAGCCCGCC

Downstream 100 bases:

>100_bases
GGAGTAGCGATGCGCTGCCCCTATTGCGGTTCGGAAGATACACAGGTTAAAGATTCGCGTCCGGCGGAGGACAATACGTC
CATCCGCCGGCGGCGTATCT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTNASTESFFNRSLADVDPEIFGAIGKELGRQRHEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGGCQFVDIA
EELAIERAKKLFGVNFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREGDNLL
DMDEVERKAKETKPKLIIAGGTAYSRIWDWKRFREIADSVGAYLMVDMAHIAGLVAGGQHPSPFPHCHVATTTTHKSLRG
PRGGVILTNEEDLAKKFNSAVFPGLQGGPLMHIIAAKAVAFGEALQPEFKDYAAQVVKNAKALAETLIAGGLDVVSGGTD
NHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEKPFVTSGVRLGAPAGTTRGFKEAEFREIGNLIVEVLDGLKA
ANSDEGNAAVEAAVRGKVVNLTDRFPMYDYMG

Sequences:

>Translated_432_residues
MTNASTESFFNRSLADVDPEIFGAIGKELGRQRHEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGGCQFVDIA
EELAIERAKKLFGVNFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREGDNLL
DMDEVERKAKETKPKLIIAGGTAYSRIWDWKRFREIADSVGAYLMVDMAHIAGLVAGGQHPSPFPHCHVATTTTHKSLRG
PRGGVILTNEEDLAKKFNSAVFPGLQGGPLMHIIAAKAVAFGEALQPEFKDYAAQVVKNAKALAETLIAGGLDVVSGGTD
NHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEKPFVTSGVRLGAPAGTTRGFKEAEFREIGNLIVEVLDGLKA
ANSDEGNAAVEAAVRGKVVNLTDRFPMYDYMG
>Mature_431_residues
TNASTESFFNRSLADVDPEIFGAIGKELGRQRHEIELIASENIVSRAVLEAQGSIMTNKYAEGYPGKRYYGGCQFVDIAE
ELAIERAKKLFGVNFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREGDNLLD
MDEVERKAKETKPKLIIAGGTAYSRIWDWKRFREIADSVGAYLMVDMAHIAGLVAGGQHPSPFPHCHVATTTTHKSLRGP
RGGVILTNEEDLAKKFNSAVFPGLQGGPLMHIIAAKAVAFGEALQPEFKDYAAQVVKNAKALAETLIAGGLDVVSGGTDN
HLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEKPFVTSGVRLGAPAGTTRGFKEAEFREIGNLIVEVLDGLKAA
NSDEGNAAVEAAVRGKVVNLTDRFPMYDYMG

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=404, Percent_Identity=48.2673267326733, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI261862350, Length=404, Percent_Identity=48.2673267326733, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI261862348, Length=404, Percent_Identity=48.2673267326733, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI19923315, Length=404, Percent_Identity=48.2673267326733, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI261862346, Length=404, Percent_Identity=47.7722772277228, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI22547186, Length=464, Percent_Identity=45.2586206896552, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI22547189, Length=450, Percent_Identity=42.6666666666667, Blast_Score=310, Evalue=2e-84,
Organism=Escherichia coli, GI1788902, Length=418, Percent_Identity=60.5263157894737, Blast_Score=494, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI25144729, Length=424, Percent_Identity=48.3490566037736, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25144732, Length=412, Percent_Identity=49.5145631067961, Blast_Score=366, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319739, Length=425, Percent_Identity=44.2352941176471, Blast_Score=355, Evalue=6e-99,
Organism=Saccharomyces cerevisiae, GI6323087, Length=401, Percent_Identity=46.6334164588529, Blast_Score=339, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24640005, Length=424, Percent_Identity=46.4622641509434, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=418, Percent_Identity=46.88995215311, Blast_Score=360, Evalue=1e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 46549; Mature: 46418

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNASTESFFNRSLADVDPEIFGAIGKELGRQRHEIELIASENIVSRAVLEAQGSIMTNK
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHEEEEEECCCHHHHHHHHHCCCEEECC
YAEGYPGKRYYGGCQFVDIAEELAIERAKKLFGVNFANVQPNSGSQMNQAVFLALLQPGD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCHHHHEEEEEEECCCC
TFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREGDNLLDMDEVERKAKETKPKLIIAG
EEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEC
GTAYSRIWDWKRFREIADSVGAYLMVDMAHIAGLVAGGQHPSPFPHCHVATTTTHKSLRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHCC
PRGGVILTNEEDLAKKFNSAVFPGLQGGPLMHIIAAKAVAFGEALQPEFKDYAAQVVKNA
CCCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KALAETLIAGGLDVVSGGTDNHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEK
HHHHHHHHHCCCCEECCCCCCEEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCCCCCC
PFVTSGVRLGAPAGTTRGFKEAEFREIGNLIVEVLDGLKAANSDEGNAAVEAAVRGKVVN
CCEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEE
LTDRFPMYDYMG
ECCCCCCHHCCC
>Mature Secondary Structure 
TNASTESFFNRSLADVDPEIFGAIGKELGRQRHEIELIASENIVSRAVLEAQGSIMTNK
CCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHEEEEEECCCHHHHHHHHHCCCEEECC
YAEGYPGKRYYGGCQFVDIAEELAIERAKKLFGVNFANVQPNSGSQMNQAVFLALLQPGD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCHHHHEEEEEEECCCC
TFMGLDLNSGGHLTHGSPVNMSGKWFNVVSYGVREGDNLLDMDEVERKAKETKPKLIIAG
EEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEC
GTAYSRIWDWKRFREIADSVGAYLMVDMAHIAGLVAGGQHPSPFPHCHVATTTTHKSLRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHCC
PRGGVILTNEEDLAKKFNSAVFPGLQGGPLMHIIAAKAVAFGEALQPEFKDYAAQVVKNA
CCCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KALAETLIAGGLDVVSGGTDNHLMLVDLRKKNATGKRAEAALGRAYVTCNKNGIPFDPEK
HHHHHHHHHCCCCEECCCCCCEEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCCCCCC
PFVTSGVRLGAPAGTTRGFKEAEFREIGNLIVEVLDGLKAANSDEGNAAVEAAVRGKVVN
CCEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEE
LTDRFPMYDYMG
ECCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA