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The map label for this gene is purN [H]

Identifier: 209548697

GI number: 209548697

Start: 1113929

End: 1114600

Strand: Reverse

Name: purN [H]

Synonym: Rleg2_1094

Alternate gene names: 209548697

Gene position: 1114600-1113929 (Counterclockwise)

Preceding gene: 209548698

Following gene: 209548693

Centisome position: 24.56

GC content: 64.43

Gene sequence:

>672_bases
ATGAGCGCGAAGCGCAAACGCGTCGTTGTCCTCATATCCGGCAGCGGCTCCAACATGATGGCGCTGGTTGCGGCGGCCAA
GGCTGCCGACTATCCAGCCGAAATCGTCGGGGTGATCTCCGACAAGGCGGATGCCGGCGGACTTGCCAAGGCGGCGGCCG
AAGGCATCGCCACCTTCGCCTTCCCTCGCAAGGATTTTGCCAGCAAGGATGCGCATGAGGCGGCGATCTTTTCCGCACTC
GATGAGCTTTCTCCCGACATCCTCTGCCTCGCCGGCTATATGCGGCTGCTGACCGCGACCTTCATTCAGCGTTATGAGGG
CCGGATGCTCAACATCCACCCTTCCCTGCTGCCGCTGTTTCCCGGCCTGCACACCCATCAGCGCGCCATCGACGCCGGCA
TGCGCATCGCCGGCTGCACCGTGCATTTCGTCACCGAAGGCATGGACGAAGGACCGGTGATCGGCCAGGCGGCCGTCCCA
ATCCTTTCCGGTGACACGGCCGAAAGCCTCGCCGCGCGCGTGCTCACCGTCGAACACCAGATCTACCCGCAGGCGCTGCG
GCTCTTTGCCGAAGGCCGCGTGAGCATGGAAGGCGGAAAAGCCGTTGGCGCCGAAGCTTCGGCCGTTGCGCCCAAGGCTC
AGCTCATCTCGCTGATCGGCGATCGCGCTTAG

Upstream 100 bases:

>100_bases
CCGCACTCGAGGCCGAGGGCGAAACCGTCGTCACGCTCGGCCGCATGATCGCCCGAGACGAGGGTGCAGCCGGCACGGTC
TATCAGGGCACGCTTGCCCT

Downstream 100 bases:

>100_bases
GCCGCCAGCGCCCGCAACGGGTGCAGCGTGCCGTCGCTGTAGACCAAGCGGCCGGCGCGGAAGGTGGCGCGGATGCGGTT
GATGTCGCCCGGTTCGACCG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSAKRKRVVVLISGSGSNMMALVAAAKAADYPAEIVGVISDKADAGGLAKAAAEGIATFAFPRKDFASKDAHEAAIFSAL
DELSPDILCLAGYMRLLTATFIQRYEGRMLNIHPSLLPLFPGLHTHQRAIDAGMRIAGCTVHFVTEGMDEGPVIGQAAVP
ILSGDTAESLAARVLTVEHQIYPQALRLFAEGRVSMEGGKAVGAEASAVAPKAQLISLIGDRA

Sequences:

>Translated_223_residues
MSAKRKRVVVLISGSGSNMMALVAAAKAADYPAEIVGVISDKADAGGLAKAAAEGIATFAFPRKDFASKDAHEAAIFSAL
DELSPDILCLAGYMRLLTATFIQRYEGRMLNIHPSLLPLFPGLHTHQRAIDAGMRIAGCTVHFVTEGMDEGPVIGQAAVP
ILSGDTAESLAARVLTVEHQIYPQALRLFAEGRVSMEGGKAVGAEASAVAPKAQLISLIGDRA
>Mature_222_residues
SAKRKRVVVLISGSGSNMMALVAAAKAADYPAEIVGVISDKADAGGLAKAAAEGIATFAFPRKDFASKDAHEAAIFSALD
ELSPDILCLAGYMRLLTATFIQRYEGRMLNIHPSLLPLFPGLHTHQRAIDAGMRIAGCTVHFVTEGMDEGPVIGQAAVPI
LSGDTAESLAARVLTVEHQIYPQALRLFAEGRVSMEGGKAVGAEASAVAPKAQLISLIGDRA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=198, Percent_Identity=47.4747474747475, Blast_Score=187, Evalue=5e-48,
Organism=Homo sapiens, GI209869995, Length=198, Percent_Identity=47.4747474747475, Blast_Score=187, Evalue=5e-48,
Organism=Homo sapiens, GI209869993, Length=198, Percent_Identity=47.4747474747475, Blast_Score=187, Evalue=5e-48,
Organism=Escherichia coli, GI1788846, Length=195, Percent_Identity=46.1538461538462, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1787483, Length=200, Percent_Identity=29, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17567511, Length=183, Percent_Identity=40.9836065573771, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=34.6733668341709, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24582400, Length=194, Percent_Identity=46.9072164948454, Blast_Score=188, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23311; Mature: 23180

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKRKRVVVLISGSGSNMMALVAAAKAADYPAEIVGVISDKADAGGLAKAAAEGIATFA
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHC
FPRKDFASKDAHEAAIFSALDELSPDILCLAGYMRLLTATFIQRYEGRMLNIHPSLLPLF
CCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHC
PGLHTHQRAIDAGMRIAGCTVHFVTEGMDEGPVIGQAAVPILSGDTAESLAARVLTVEHQ
CCCCHHHHHHHHCCEEECEEEEEEECCCCCCCCCCHHCCCEECCCCHHHHHHHHHHHHHH
IYPQALRLFAEGRVSMEGGKAVGAEASAVAPKAQLISLIGDRA
HHHHHHHHHHCCCEEECCCCCCCCCCHHHCHHHHHHHHHCCCC
>Mature Secondary Structure 
SAKRKRVVVLISGSGSNMMALVAAAKAADYPAEIVGVISDKADAGGLAKAAAEGIATFA
CCCCCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHC
FPRKDFASKDAHEAAIFSALDELSPDILCLAGYMRLLTATFIQRYEGRMLNIHPSLLPLF
CCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHC
PGLHTHQRAIDAGMRIAGCTVHFVTEGMDEGPVIGQAAVPILSGDTAESLAARVLTVEHQ
CCCCHHHHHHHHCCEEECEEEEEEECCCCCCCCCCHHCCCEECCCCHHHHHHHHHHHHHH
IYPQALRLFAEGRVSMEGGKAVGAEASAVAPKAQLISLIGDRA
HHHHHHHHHHCCCEEECCCCCCCCCCHHHCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]