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The map label for this gene is ksgA [H]

Identifier: 209548666

GI number: 209548666

Start: 1080724

End: 1081551

Strand: Reverse

Name: ksgA [H]

Synonym: Rleg2_1063

Alternate gene names: 209548666

Gene position: 1081551-1080724 (Counterclockwise)

Preceding gene: 209548667

Following gene: 209548658

Centisome position: 23.83

GC content: 65.82

Gene sequence:

>828_bases
ATGGCAGCACTCGATGGCCTGCCGCCGCTTCGCGACGTCATCCAGCGTCACGGCCTCGATGCGCGCAAGGCGCTCGGGCA
GAACTTCCTGCTCGACCTTAACCTCACGCAGAAGATCGCCCGCACGGCGGGTGCCCTCGAAGAGGCGACCATCTTCGAGG
TCGGCCCCGGCCCCGGCGGGCTGACACGGGCGATCCTGGCGCTCGGCGCCCGCAAGGTCATCGCCGTCGAGCGCGATACG
CGCTGCCTGCCGGCACTCGCCGAGATCGCCGATCACTATCCCGGCCGGCTCGAGGTGATCGAAGGCGATGCGCTGAAGAC
CGATTTCGAGGCCTTGGCGCCTGATGGACCGATCAAGATCATCGCCAACCTCCCCTACAACGTCGGTACGCAGCTTCTGG
TCAACTGGCTGCTGCCGAAAGCCTGGCCGCCATTCTGGCAGTCGCTGACGCTGATGTTTCAGAAGGAAGTCGGCGAGCGT
ATCGTCGCCTCTGAAGACGACGACCATTATGGCCGGCTCGGCGTGCTCTGCGGCTGGCGGACGCAGACGCGCATGGCCTT
CGACGTGCCGCCGCAAGCCTTCACGCCGCCGCCGAAGGTCACGTCGACGGTCGTGCATCTGACGCCGCGCGAGAACCCCA
TCCCCTGCGCTGTCGGTGATCTGGAAAAGGTAACGCAGGCAGCCTTCGGCCAGCGCCGCAAGATGCTGCGCCAAAGCCTG
AAACCGCTTGGCGGCGAGAGGCTTCTCGTCAAGGCCGGGATCGATCCGGCGCGCCGGGCGGAGACCTTGTCAGTCGAGGA
ATTCTGCCTTCTCGCAAACAGCCTCTAA

Upstream 100 bases:

>100_bases
CTTTCGGCATCGCCGGCAAGGGACTGGCTCGCGAACAGAGCCTGGTTGCAGCGCTGAAGCTCGCCGCCCAGCTCGGCCGC
ACTGCGGAAAGCCACCGCTG

Downstream 100 bases:

>100_bases
AGCGACGGCGTCTCTTCCAGCAGCTCGCGGACGAAATCGAACATGCCGTGGCGGCGGTCGCGGCGCAAGCGTTCGGCCTT
GACGATCGACTGGACGGCGT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MAALDGLPPLRDVIQRHGLDARKALGQNFLLDLNLTQKIARTAGALEEATIFEVGPGPGGLTRAILALGARKVIAVERDT
RCLPALAEIADHYPGRLEVIEGDALKTDFEALAPDGPIKIIANLPYNVGTQLLVNWLLPKAWPPFWQSLTLMFQKEVGER
IVASEDDDHYGRLGVLCGWRTQTRMAFDVPPQAFTPPPKVTSTVVHLTPRENPIPCAVGDLEKVTQAAFGQRRKMLRQSL
KPLGGERLLVKAGIDPARRAETLSVEEFCLLANSL

Sequences:

>Translated_275_residues
MAALDGLPPLRDVIQRHGLDARKALGQNFLLDLNLTQKIARTAGALEEATIFEVGPGPGGLTRAILALGARKVIAVERDT
RCLPALAEIADHYPGRLEVIEGDALKTDFEALAPDGPIKIIANLPYNVGTQLLVNWLLPKAWPPFWQSLTLMFQKEVGER
IVASEDDDHYGRLGVLCGWRTQTRMAFDVPPQAFTPPPKVTSTVVHLTPRENPIPCAVGDLEKVTQAAFGQRRKMLRQSL
KPLGGERLLVKAGIDPARRAETLSVEEFCLLANSL
>Mature_274_residues
AALDGLPPLRDVIQRHGLDARKALGQNFLLDLNLTQKIARTAGALEEATIFEVGPGPGGLTRAILALGARKVIAVERDTR
CLPALAEIADHYPGRLEVIEGDALKTDFEALAPDGPIKIIANLPYNVGTQLLVNWLLPKAWPPFWQSLTLMFQKEVGERI
VASEDDDHYGRLGVLCGWRTQTRMAFDVPPQAFTPPPKVTSTVVHLTPRENPIPCAVGDLEKVTQAAFGQRRKMLRQSLK
PLGGERLLVKAGIDPARRAETLSVEEFCLLANSL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=291, Percent_Identity=40.893470790378, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI7657198, Length=236, Percent_Identity=30.9322033898305, Blast_Score=104, Evalue=9e-23,
Organism=Escherichia coli, GI1786236, Length=266, Percent_Identity=36.4661654135338, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25141369, Length=304, Percent_Identity=34.2105263157895, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25146882, Length=216, Percent_Identity=31.9444444444444, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=193, Percent_Identity=34.1968911917098, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21357273, Length=289, Percent_Identity=34.2560553633218, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21358017, Length=232, Percent_Identity=32.7586206896552, Blast_Score=107, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30074; Mature: 29943

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALDGLPPLRDVIQRHGLDARKALGQNFLLDLNLTQKIARTAGALEEATIFEVGPGPGG
CCCCCCCCHHHHHHHHCCCHHHHHCCCCEEEECCHHHHHHHHHCCCCCCEEEEECCCCCH
LTRAILALGARKVIAVERDTRCLPALAEIADHYPGRLEVIEGDALKTDFEALAPDGPIKI
HHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHCCCCCEEE
IANLPYNVGTQLLVNWLLPKAWPPFWQSLTLMFQKEVGERIVASEDDDHYGRLGVLCGWR
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
TQTRMAFDVPPQAFTPPPKVTSTVVHLTPRENPIPCAVGDLEKVTQAAFGQRRKMLRQSL
CCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KPLGGERLLVKAGIDPARRAETLSVEEFCLLANSL
CCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
AALDGLPPLRDVIQRHGLDARKALGQNFLLDLNLTQKIARTAGALEEATIFEVGPGPGG
CCCCCCCHHHHHHHHCCCHHHHHCCCCEEEECCHHHHHHHHHCCCCCCEEEEECCCCCH
LTRAILALGARKVIAVERDTRCLPALAEIADHYPGRLEVIEGDALKTDFEALAPDGPIKI
HHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHCCCCCEEE
IANLPYNVGTQLLVNWLLPKAWPPFWQSLTLMFQKEVGERIVASEDDDHYGRLGVLCGWR
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
TQTRMAFDVPPQAFTPPPKVTSTVVHLTPRENPIPCAVGDLEKVTQAAFGQRRKMLRQSL
CCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KPLGGERLLVKAGIDPARRAETLSVEEFCLLANSL
CCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA