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The map label for this gene is purF [H]

Identifier: 209548645

GI number: 209548645

Start: 1060279

End: 1061769

Strand: Reverse

Name: purF [H]

Synonym: Rleg2_1042

Alternate gene names: 209548645

Gene position: 1061769-1060279 (Counterclockwise)

Preceding gene: 209548646

Following gene: 209548644

Centisome position: 23.4

GC content: 62.17

Gene sequence:

>1491_bases
ATGAACCAGTCCCATTCCTTGCCCACCGACGATCCGCTCGACGGAGATACGCTGCATGAAGAATGCGGGGTTTTCGGAAT
TCTGGGACATCCCGATGCCGCGGCACTCACGGCTCTCGGCCTGCATGCTCTGCAGCATCGCGGGCAGGAAGCGGCCGGCA
TCGTCTCCTTCGATGGCAAGCGCTTCTACCAGGAGCGCCATATGGGCCTTGTCGGCGACCACTATACCAATCCGATGACA
CTCGCCCGCCTGCCCGGCAGCATATCGATCGGTCATACGCGCTACTCGACAACTGGCGAAGTGGCGATGCGCAACGTGCA
GCCGCTCTTTGCCGAACTTGAGGAAGGCGGCATTGCCGTTGCCCATAACGGCAATTTCACCAACGGGCTGACGCTGCGCC
GCCAGATCATCGCGACCGGCGCCATCTGTCAGTCGACCTCCGATACCGAAGTGGTCCTCCACCTCATCGCCCGCTCCCGT
CACGCCTCCACATCCGACCGCTTCATCGACGCCATCCGCCAGATGGAGGGCGGCTATTCGATGCTCGCGATGACGCGCAC
CAAGCTGATTGCCGCACGTGACCCCACAGGCATCCGCCCGCTTGTGATGGGCGAACTCGATGGTAAGCCGATCTTCTGCT
CGGAAACCTGCGCGCTCGACATTATCGGCGCCAAGTTCATCCGCGATGTCGAAAATGGCGAGGTCGTCATCTGCGAAATC
CAGCCCGACGGCTCGATCAGCATCGATGCGCGCAAGCCCGGCAAGCCGCAGCCGGAGCGCCTCTGCCTGTTCGAATATGT
CTATTTCGCCCGGCCCGACTCAGTCGTCGGCGGCCGCAACGTCTATACGACGCGCAAGAATATGGGCATGAACCTCGCCA
AGGAGTCGCCTGTCGAGGCCGACGTGGTCGTGCCGGTGCCGGATGGCGGCACGCCGGCGGCGCTCGGCTATGCACAGGAA
AGCGGCATTCCCTTCGAATACGGCATCATCCGTAACCACTATGTCGGCCGCACCTTCATCGAGCCGACCCAGCAGATCCG
CGCCTTCGGCGTGAAGCTGAAGCATTCCGCCAACCGGGCGATGATCGAAGGCAAGCGCGTGGTGCTGGTCGATGATTCCA
TCGTGCGCGGCACGACGTCGCTGAAGATCGTGCAGATGATCCGTGAGGCCGGCGCGCGCGAAGTTCATCTCCGCGTCGCC
AGCCCGATGATCTTCTTCCCCGACTTCTACGGCATCGACACGCCCGATGCCGAAAAGCTCTTGGCCAACCAGTATGCCGA
TGTCGAAGCCATGGCCAAATATATCGGCGCGGATTCGCTGGCCTTCCTGTCGATCAACGGGCTCTACCGCGCCGTCGGCG
GCGAGGATCGCAATTCGGCCCGCCCGCAGTTCACCGACCACTACTTCACCGGTGACTATCCGACCCGCCTGCTCGACAAG
AATGGCGAGTCGATGGGCAACAAGCTTTCGATGCTTGCCAGCAACGGCTGA

Upstream 100 bases:

>100_bases
GGGACGAAATGAGGGGTCCGGCCGGGCATTCCAGCTCTGTCGGGCTTCAGCTATTTCTGGAAATGCCGATCCATTCTTGT
GAGAGCAGAGGCCCGCAGAA

Downstream 100 bases:

>100_bases
GCCGCTTCCGTTTTGACTTTCCTATCAGGGGCGCTTATTGCGCCCCTTACCTTTTCAAGCACAGCCGGGACCGGAGCAGA
TGAACATCAATCTTGAAGGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MNQSHSLPTDDPLDGDTLHEECGVFGILGHPDAAALTALGLHALQHRGQEAAGIVSFDGKRFYQERHMGLVGDHYTNPMT
LARLPGSISIGHTRYSTTGEVAMRNVQPLFAELEEGGIAVAHNGNFTNGLTLRRQIIATGAICQSTSDTEVVLHLIARSR
HASTSDRFIDAIRQMEGGYSMLAMTRTKLIAARDPTGIRPLVMGELDGKPIFCSETCALDIIGAKFIRDVENGEVVICEI
QPDGSISIDARKPGKPQPERLCLFEYVYFARPDSVVGGRNVYTTRKNMGMNLAKESPVEADVVVPVPDGGTPAALGYAQE
SGIPFEYGIIRNHYVGRTFIEPTQQIRAFGVKLKHSANRAMIEGKRVVLVDDSIVRGTTSLKIVQMIREAGAREVHLRVA
SPMIFFPDFYGIDTPDAEKLLANQYADVEAMAKYIGADSLAFLSINGLYRAVGGEDRNSARPQFTDHYFTGDYPTRLLDK
NGESMGNKLSMLASNG

Sequences:

>Translated_496_residues
MNQSHSLPTDDPLDGDTLHEECGVFGILGHPDAAALTALGLHALQHRGQEAAGIVSFDGKRFYQERHMGLVGDHYTNPMT
LARLPGSISIGHTRYSTTGEVAMRNVQPLFAELEEGGIAVAHNGNFTNGLTLRRQIIATGAICQSTSDTEVVLHLIARSR
HASTSDRFIDAIRQMEGGYSMLAMTRTKLIAARDPTGIRPLVMGELDGKPIFCSETCALDIIGAKFIRDVENGEVVICEI
QPDGSISIDARKPGKPQPERLCLFEYVYFARPDSVVGGRNVYTTRKNMGMNLAKESPVEADVVVPVPDGGTPAALGYAQE
SGIPFEYGIIRNHYVGRTFIEPTQQIRAFGVKLKHSANRAMIEGKRVVLVDDSIVRGTTSLKIVQMIREAGAREVHLRVA
SPMIFFPDFYGIDTPDAEKLLANQYADVEAMAKYIGADSLAFLSINGLYRAVGGEDRNSARPQFTDHYFTGDYPTRLLDK
NGESMGNKLSMLASNG
>Mature_496_residues
MNQSHSLPTDDPLDGDTLHEECGVFGILGHPDAAALTALGLHALQHRGQEAAGIVSFDGKRFYQERHMGLVGDHYTNPMT
LARLPGSISIGHTRYSTTGEVAMRNVQPLFAELEEGGIAVAHNGNFTNGLTLRRQIIATGAICQSTSDTEVVLHLIARSR
HASTSDRFIDAIRQMEGGYSMLAMTRTKLIAARDPTGIRPLVMGELDGKPIFCSETCALDIIGAKFIRDVENGEVVICEI
QPDGSISIDARKPGKPQPERLCLFEYVYFARPDSVVGGRNVYTTRKNMGMNLAKESPVEADVVVPVPDGGTPAALGYAQE
SGIPFEYGIIRNHYVGRTFIEPTQQIRAFGVKLKHSANRAMIEGKRVVLVDDSIVRGTTSLKIVQMIREAGAREVHLRVA
SPMIFFPDFYGIDTPDAEKLLANQYADVEAMAKYIGADSLAFLSINGLYRAVGGEDRNSARPQFTDHYFTGDYPTRLLDK
NGESMGNKLSMLASNG

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=465, Percent_Identity=40.4301075268817, Blast_Score=333, Evalue=2e-91,
Organism=Escherichia coli, GI1788651, Length=481, Percent_Identity=40.1247401247401, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1790167, Length=220, Percent_Identity=30.4545454545455, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=484, Percent_Identity=39.0495867768595, Blast_Score=297, Evalue=7e-81,
Organism=Saccharomyces cerevisiae, GI6323958, Length=507, Percent_Identity=35.3057199211045, Blast_Score=243, Evalue=6e-65,
Organism=Drosophila melanogaster, GI28573187, Length=525, Percent_Identity=39.8095238095238, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24659598, Length=499, Percent_Identity=39.6793587174349, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24659604, Length=471, Percent_Identity=40.1273885350318, Blast_Score=325, Evalue=6e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54088; Mature: 54088

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSHSLPTDDPLDGDTLHEECGVFGILGHPDAAALTALGLHALQHRGQEAAGIVSFDGK
CCCCCCCCCCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCH
RFYQERHMGLVGDHYTNPMTLARLPGSISIGHTRYSTTGEVAMRNVQPLFAELEEGGIAV
HHHHHHCCCCCCCCCCCCEEEEECCCCEEECCEECCCCHHHHHHHHHHHHHHHHCCCEEE
AHNGNFTNGLTLRRQIIATGAICQSTSDTEVVLHLIARSRHASTSDRFIDAIRQMEGGYS
EECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCE
MLAMTRTKLIAARDPTGIRPLVMGELDGKPIFCSETCALDIIGAKFIRDVENGEVVICEI
EEEEHHEEEEEECCCCCCCEEEEECCCCCEEEECCCCEEEHHHHHHHHHCCCCCEEEEEE
QPDGSISIDARKPGKPQPERLCLFEYVYFARPDSVVGGRNVYTTRKNMGMNLAKESPVEA
CCCCCEEEECCCCCCCCHHHEEHHHHHHHCCCCCCCCCCCEEEEHHCCCCCCCCCCCCCC
DVVVPVPDGGTPAALGYAQESGIPFEYGIIRNHYVGRTFIEPTQQIRAFGVKLKHSANRA
EEEEECCCCCCCCEECCHHHCCCCEEECCHHCCCCCCHHCCHHHHHHHHCEEEECCCCCE
MIEGKRVVLVDDSIVRGTTSLKIVQMIREAGAREVHLRVASPMIFFPDFYGIDTPDAEKL
EECCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCHHHH
LANQYADVEAMAKYIGADSLAFLSINGLYRAVGGEDRNSARPQFTDHYFTGDYPTRLLDK
HHHHCCCHHHHHHHHCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEECCCCCHHHHCC
NGESMGNKLSMLASNG
CCHHHCCEEEEEECCC
>Mature Secondary Structure
MNQSHSLPTDDPLDGDTLHEECGVFGILGHPDAAALTALGLHALQHRGQEAAGIVSFDGK
CCCCCCCCCCCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCH
RFYQERHMGLVGDHYTNPMTLARLPGSISIGHTRYSTTGEVAMRNVQPLFAELEEGGIAV
HHHHHHCCCCCCCCCCCCEEEEECCCCEEECCEECCCCHHHHHHHHHHHHHHHHCCCEEE
AHNGNFTNGLTLRRQIIATGAICQSTSDTEVVLHLIARSRHASTSDRFIDAIRQMEGGYS
EECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCE
MLAMTRTKLIAARDPTGIRPLVMGELDGKPIFCSETCALDIIGAKFIRDVENGEVVICEI
EEEEHHEEEEEECCCCCCCEEEEECCCCCEEEECCCCEEEHHHHHHHHHCCCCCEEEEEE
QPDGSISIDARKPGKPQPERLCLFEYVYFARPDSVVGGRNVYTTRKNMGMNLAKESPVEA
CCCCCEEEECCCCCCCCHHHEEHHHHHHHCCCCCCCCCCCEEEEHHCCCCCCCCCCCCCC
DVVVPVPDGGTPAALGYAQESGIPFEYGIIRNHYVGRTFIEPTQQIRAFGVKLKHSANRA
EEEEECCCCCCCCEECCHHHCCCCEEECCHHCCCCCCHHCCHHHHHHHHCEEEECCCCCE
MIEGKRVVLVDDSIVRGTTSLKIVQMIREAGAREVHLRVASPMIFFPDFYGIDTPDAEKL
EECCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCHHHH
LANQYADVEAMAKYIGADSLAFLSINGLYRAVGGEDRNSARPQFTDHYFTGDYPTRLLDK
HHHHCCCHHHHHHHHCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEECCCCCHHHHCC
NGESMGNKLSMLASNG
CCHHHCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA