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The map label for this gene is mscS [H]

Identifier: 209548613

GI number: 209548613

Start: 1032241

End: 1033119

Strand: Reverse

Name: mscS [H]

Synonym: Rleg2_1010

Alternate gene names: 209548613

Gene position: 1033119-1032241 (Counterclockwise)

Preceding gene: 209548614

Following gene: 209548610

Centisome position: 22.77

GC content: 62.57

Gene sequence:

>879_bases
ATGCAACAACAAGCAGCCGACGCCCTTCTTGCCACGCAAACGGCGCTCAGCCAGGCGAGCGCGCTGGCGGTGCAATATTC
TTTTTCCGTCCTTGGGGCGGTGATCCTGCTCGTTCTCGGCTGGGCGCTTGCCGGTTTTGCCAGCCGCTGGGCCTATGAAG
GCCTGTCGCGCGTCCATGGCATCGATGAGACGCTGGCGCGATTCTTCACCAATGTGCTGCGATACGCGCTGCTCATCCTG
GTGTTCATCACCGTGCTCGGCCAGTTCGGTGTCCAGACCGCCTCGATCATCGCGGCCCTCGGCGCAGCCGGCCTGGCGAT
CGGACTGGCGCTGCAGGGAACGCTGCAGAATATCGCTGCCGGCATCATGCTGCTGATCCTCAGGCCGTTCCGGGTCGGGG
AATATATCGAGACCAGCAGCGTGGCCGGCACGGTGCGCGAAATCGGGCTGTTTGCGACCGAACTCAGGACTGCCGACGGG
CTCTACCGGTTGGCGCCGAATTCGACGCTCTGGAACACGCCGATCACCAATTTCAGCCGCGAGGCGACGCGGCGGAACGA
GTTGAAGATCAAGGTCGAGGATGACATCGATCAGTCGATGGAAACATTGATGGACCTTGCCAAAGCCGATTCCCGGGTGC
TGAAGTCACCGGAACCGAGCGTCTTCATCGACGGCCTCGGTGACGGCGCGATCTTCGTGACGCTGCGCTATTGGGCAAAG
ACCGGTGACTGGTGGACGGTCAGCCGGGACATGGCAAAACGCGTAAAACTCGCCTTCGACGAGAACAATCCGGCCGCGTT
GCCGGATGCACCGAACGCCGCGCCGGCAAAGACGTCCAACGGCAGGTCGGCCGCCGAAAAGCCGGCACTGAGACAGTGA

Upstream 100 bases:

>100_bases
GGTAACGCCCCCTTCGTGCTTGCGATCGGCGAGCCCGCCTGCGGTTGACGCTGCCGCCATCTGCCCCATCTAGGCCCGAC
CATGTAGAAAAGGCCAGATC

Downstream 100 bases:

>100_bases
TCCAGGAACTAATGCAGCAGACTATTGAATGACGCCGCAGGACAATCTCTCCCCGGCATCACCCGAAGGCTGGCTGCGAT
AGTCATCCGAATTGGCGTGC

Product: MscS Mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MQQQAADALLATQTALSQASALAVQYSFSVLGAVILLVLGWALAGFASRWAYEGLSRVHGIDETLARFFTNVLRYALLIL
VFITVLGQFGVQTASIIAALGAAGLAIGLALQGTLQNIAAGIMLLILRPFRVGEYIETSSVAGTVREIGLFATELRTADG
LYRLAPNSTLWNTPITNFSREATRRNELKIKVEDDIDQSMETLMDLAKADSRVLKSPEPSVFIDGLGDGAIFVTLRYWAK
TGDWWTVSRDMAKRVKLAFDENNPAALPDAPNAAPAKTSNGRSAAEKPALRQ

Sequences:

>Translated_292_residues
MQQQAADALLATQTALSQASALAVQYSFSVLGAVILLVLGWALAGFASRWAYEGLSRVHGIDETLARFFTNVLRYALLIL
VFITVLGQFGVQTASIIAALGAAGLAIGLALQGTLQNIAAGIMLLILRPFRVGEYIETSSVAGTVREIGLFATELRTADG
LYRLAPNSTLWNTPITNFSREATRRNELKIKVEDDIDQSMETLMDLAKADSRVLKSPEPSVFIDGLGDGAIFVTLRYWAK
TGDWWTVSRDMAKRVKLAFDENNPAALPDAPNAAPAKTSNGRSAAEKPALRQ
>Mature_292_residues
MQQQAADALLATQTALSQASALAVQYSFSVLGAVILLVLGWALAGFASRWAYEGLSRVHGIDETLARFFTNVLRYALLIL
VFITVLGQFGVQTASIIAALGAAGLAIGLALQGTLQNIAAGIMLLILRPFRVGEYIETSSVAGTVREIGLFATELRTADG
LYRLAPNSTLWNTPITNFSREATRRNELKIKVEDDIDQSMETLMDLAKADSRVLKSPEPSVFIDGLGDGAIFVTLRYWAK
TGDWWTVSRDMAKRVKLAFDENNPAALPDAPNAAPAKTSNGRSAAEKPALRQ

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=242, Percent_Identity=42.1487603305785, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787591, Length=191, Percent_Identity=25.6544502617801, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786670, Length=216, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367355, Length=219, Percent_Identity=22.8310502283105, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 31502; Mature: 31502

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQAADALLATQTALSQASALAVQYSFSVLGAVILLVLGWALAGFASRWAYEGLSRVHG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IDETLARFFTNVLRYALLILVFITVLGQFGVQTASIIAALGAAGLAIGLALQGTLQNIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIMLLILRPFRVGEYIETSSVAGTVREIGLFATELRTADGLYRLAPNSTLWNTPITNFSR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
EATRRNELKIKVEDDIDQSMETLMDLAKADSRVLKSPEPSVFIDGLGDGAIFVTLRYWAK
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEEHHC
TGDWWTVSRDMAKRVKLAFDENNPAALPDAPNAAPAKTSNGRSAAEKPALRQ
CCCEEEECHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MQQQAADALLATQTALSQASALAVQYSFSVLGAVILLVLGWALAGFASRWAYEGLSRVHG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IDETLARFFTNVLRYALLILVFITVLGQFGVQTASIIAALGAAGLAIGLALQGTLQNIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIMLLILRPFRVGEYIETSSVAGTVREIGLFATELRTADGLYRLAPNSTLWNTPITNFSR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
EATRRNELKIKVEDDIDQSMETLMDLAKADSRVLKSPEPSVFIDGLGDGAIFVTLRYWAK
HHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEEHHC
TGDWWTVSRDMAKRVKLAFDENNPAALPDAPNAAPAKTSNGRSAAEKPALRQ
CCCEEEECHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]