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The map label for this gene is fixK [H]

Identifier: 209548603

GI number: 209548603

Start: 1022498

End: 1023250

Strand: Direct

Name: fixK [H]

Synonym: Rleg2_1000

Alternate gene names: 209548603

Gene position: 1022498-1023250 (Clockwise)

Preceding gene: 209548602

Following gene: 209548604

Centisome position: 22.53

GC content: 57.37

Gene sequence:

>753_bases
ATGGTGACGTCGAGATCGATCAATTCCTTCAAGACTCCTTGCCAGCAATGTCCTTTGCGGCCGCTGCCGCATTTCCGGGA
ATTCAGCCGCGACGAACTGGAATTCGTTTCCAATTTCAAGAGAGGTGAACTTGCCGTCGATGCCGGTTCCACCATCCTCG
TCGAGGGTGCGCATAGCGCGCATCTTTTCACTGTGCTTGCCGGTTGGGGCTTTCGCTACAAGATGCTGGAGGATGGGCGC
CGCCAGATACTGAATTATATCATGCCGGGCGATCTGATCGGCCTGCAGGGAACGATCGCCGGCGAGATGCAGCATTCCGT
CGAAGCGCTGTCACCGGTTTCTCTCTGCGTCTTCGAGCGCGACAGGCTGATGACGCTCTATAACAAACACCCCTCGCTCG
CCTTCGACATCACCTGGATCGCGGCGCGCGAGGAGCGAATCTTGGATGAACACCTGCTCAGCATCGGCCGTCGCACGGCG
TTGGAAAGAGCAGCCTATCTGATCGCTTTTCTGTTTGAGCGCGGCCGCAAGCTTAATCTCTTCAATGGCCGCCAATTCAT
CCCCATCACCCAGCAACACATTGCCGATACGCTCGGCCTTTCCATCGTCCACACCAACAAGACCTTGAAAAAGCTCGGTG
AACGCGGGTTGATCCGCTGGCAGGAGCGCGGCTGCGAGGTTCTGAACGGCGAGGAATTGATGGCGATTGCCGGCTGGGAA
GGGCTCGGAGAGAGCAAACGTCCCTTCATCTAG

Upstream 100 bases:

>100_bases
AAAAACCGCATCGCTCACGGAACAATTCGGTAACTTGTGCATTCCTTGATCAACGGCGCGCCAAAGACGGCGTGCCAGTT
TTGCGACGGCAGGGGCGGGC

Downstream 100 bases:

>100_bases
CCCCGGCCTTATGTCTTTTTTAAATGCGCATTAGCATCAGGGAAAATAAGCTCGGTCGTCGGCTTCCTCCGATTTGATTC
TTATCGAACGGGCAGAAAAA

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MVTSRSINSFKTPCQQCPLRPLPHFREFSRDELEFVSNFKRGELAVDAGSTILVEGAHSAHLFTVLAGWGFRYKMLEDGR
RQILNYIMPGDLIGLQGTIAGEMQHSVEALSPVSLCVFERDRLMTLYNKHPSLAFDITWIAAREERILDEHLLSIGRRTA
LERAAYLIAFLFERGRKLNLFNGRQFIPITQQHIADTLGLSIVHTNKTLKKLGERGLIRWQERGCEVLNGEELMAIAGWE
GLGESKRPFI

Sequences:

>Translated_250_residues
MVTSRSINSFKTPCQQCPLRPLPHFREFSRDELEFVSNFKRGELAVDAGSTILVEGAHSAHLFTVLAGWGFRYKMLEDGR
RQILNYIMPGDLIGLQGTIAGEMQHSVEALSPVSLCVFERDRLMTLYNKHPSLAFDITWIAAREERILDEHLLSIGRRTA
LERAAYLIAFLFERGRKLNLFNGRQFIPITQQHIADTLGLSIVHTNKTLKKLGERGLIRWQERGCEVLNGEELMAIAGWE
GLGESKRPFI
>Mature_250_residues
MVTSRSINSFKTPCQQCPLRPLPHFREFSRDELEFVSNFKRGELAVDAGSTILVEGAHSAHLFTVLAGWGFRYKMLEDGR
RQILNYIMPGDLIGLQGTIAGEMQHSVEALSPVSLCVFERDRLMTLYNKHPSLAFDITWIAAREERILDEHLLSIGRRTA
LERAAYLIAFLFERGRKLNLFNGRQFIPITQQHIADTLGLSIVHTNKTLKKLGERGLIRWQERGCEVLNGEELMAIAGWE
GLGESKRPFI

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=228, Percent_Identity=23.2456140350877, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 28423; Mature: 28423

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSRSINSFKTPCQQCPLRPLPHFREFSRDELEFVSNFKRGELAVDAGSTILVEGAHSA
CCCCCCCHHHCCHHHHCCCCCCCHHHHCCHHHHHHHHCCCCCCEEEECCCEEEEECCCCH
HLFTVLAGWGFRYKMLEDGRRQILNYIMPGDLIGLQGTIAGEMQHSVEALSPVSLCVFER
HHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCHHHHEEECC
DRLMTLYNKHPSLAFDITWIAAREERILDEHLLSIGRRTALERAAYLIAFLFERGRKLNL
CHHHHHHCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FNGRQFIPITQQHIADTLGLSIVHTNKTLKKLGERGLIRWQERGCEVLNGEELMAIAGWE
CCCCEECCCHHHHHHHHHCEEEEECCHHHHHHHHCCCEEEHHCCCCEECCCEEEEEECCC
GLGESKRPFI
CCCCCCCCCC
>Mature Secondary Structure
MVTSRSINSFKTPCQQCPLRPLPHFREFSRDELEFVSNFKRGELAVDAGSTILVEGAHSA
CCCCCCCHHHCCHHHHCCCCCCCHHHHCCHHHHHHHHCCCCCCEEEECCCEEEEECCCCH
HLFTVLAGWGFRYKMLEDGRRQILNYIMPGDLIGLQGTIAGEMQHSVEALSPVSLCVFER
HHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCHHHHEEECC
DRLMTLYNKHPSLAFDITWIAAREERILDEHLLSIGRRTALERAAYLIAFLFERGRKLNL
CHHHHHHCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FNGRQFIPITQQHIADTLGLSIVHTNKTLKKLGERGLIRWQERGCEVLNGEELMAIAGWE
CCCCEECCCHHHHHHHHHCEEEEECCHHHHHHHHCCCEEEHHCCCCEECCCEEEEEECCC
GLGESKRPFI
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]