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The map label for this gene is puuB [H]

Identifier: 209548466

GI number: 209548466

Start: 882498

End: 883790

Strand: Reverse

Name: puuB [H]

Synonym: Rleg2_0863

Alternate gene names: 209548466

Gene position: 883790-882498 (Counterclockwise)

Preceding gene: 209548467

Following gene: 209548463

Centisome position: 19.48

GC content: 65.27

Gene sequence:

>1293_bases
GTGGCATCTGATCTCGCTCTCAACGAAGCCGATCACGACCAGATGGTCAGCGGTCGATCGCTCTGGGGGAAAAGCGGCAT
TCGGCCGGAATGGCGGCCGATCGAGCAGAGTTTTTCCACCGACATCATCGTGATCGGCGGCGGCATCACCGGCGCCCTGG
TCGGCGAACATCTGACGGCGCGCGGTTTTTCCGTTGTTATCATCGACCGGGAGGAGCCCGGTTTCGGCAGCACCGCGGCA
AGCACGGCGATGCTGCAATGGGAAATCGACAGCACGCTCACCGAACTTGAGGACTATTACGGCTTCGAACGCGCCGCCGG
CATCTACCGCCGCAGCGGAGCAGCGGTCGCCGGCCTTTCCAAGCTGATCGCCGCGCACGGGATTGCCTGCGGCTTCCGGC
CGCGCAACACGCTGTATCTCGCCACCAACCAGGAAGGCGCGCGCGAGCTGCTGGAGGAGCGCCAGCTTCGCCGCCGGGCC
GGCCTGCCCGGCGTCTACCTCGAACATCCCGATCTCTTCACCCAGTTCGAGCTCGACCGGGATGGGGCAATCTATTCGCC
GGGTTCGGCCGAGGCCGATCCCCTGCTTCTGACCTGGGCATTGATCGAAATGTCCGTCCGGCGCGGCGCGCGGCTGGTCA
ATGCTTCCGTCACCGCGCTTCACAGCGAAGGCGATCACGTCACGGCGGAGACGGACGAAGGCCACGTCATCGAGGCGCGG
CATGCCGTGCTTGCCACCGGCTATTCGATGCCAGGCCTCGACATGCCGAAGCTGCACCGCACCAGTTCGAGCTGGGCGCT
CGCCACCGTAGCCCAGGACCCAGCAAATTTCTGGCGCGACAGGGCGCTGATCTGGGAGGACAGCCACCCCTATCTCTACA
TGCGCACGACCGAGGACAATCGCATCGTCGCCGGCGGCGAGGATGACGGCACGGCCGATCCCGAGGCCCGCGACCTCAAG
CTGCCGGCAAAGATCATGGCAATTCGCGAGAAGATAAAGCGTCTATGGCCGAAAGCCGATACGCGGGTGGACCACGCCTG
GTGCGGAACCTTCGGCGAAACGGCCGACGGCTTGCCGCTGATCGGCCCGGTGCCCAAGATGCCGCATGTGTTCGCAGCCT
ATGGCTACGGTGGCAACGGCATCACCTTTTCCTATCTCGCCGCCCAGATGATCGGCGCCATGCTGGCCGGCATGCATCGC
GACTGGTTCGAGGATTTCGCGCTGGACCGGGATGGGCCGGGGCTGCGGCGGTTTGGGGAGGATAGGTTGGGGGCTGGGAG
TGAGCTGCGGTAG

Upstream 100 bases:

>100_bases
CCTGCCGCTGTCGCTGATCGTCATCACAATCATCGAGGAATTGCTGATCGAGGAAAAGCCGCAGCCGCAATTGCCGAAAT
GATCAACCGGGGAGATAGTG

Downstream 100 bases:

>100_bases
AGGCTTCGTGGCTGTTGTGCCGTGGCAGCCCTTAAAGAATTTACTCAGTCGTCCGATCGTCACCCATATCCTCGACATCC
TCCAGACGAACGAACTCCGT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MASDLALNEADHDQMVSGRSLWGKSGIRPEWRPIEQSFSTDIIVIGGGITGALVGEHLTARGFSVVIIDREEPGFGSTAA
STAMLQWEIDSTLTELEDYYGFERAAGIYRRSGAAVAGLSKLIAAHGIACGFRPRNTLYLATNQEGARELLEERQLRRRA
GLPGVYLEHPDLFTQFELDRDGAIYSPGSAEADPLLLTWALIEMSVRRGARLVNASVTALHSEGDHVTAETDEGHVIEAR
HAVLATGYSMPGLDMPKLHRTSSSWALATVAQDPANFWRDRALIWEDSHPYLYMRTTEDNRIVAGGEDDGTADPEARDLK
LPAKIMAIREKIKRLWPKADTRVDHAWCGTFGETADGLPLIGPVPKMPHVFAAYGYGGNGITFSYLAAQMIGAMLAGMHR
DWFEDFALDRDGPGLRRFGEDRLGAGSELR

Sequences:

>Translated_430_residues
MASDLALNEADHDQMVSGRSLWGKSGIRPEWRPIEQSFSTDIIVIGGGITGALVGEHLTARGFSVVIIDREEPGFGSTAA
STAMLQWEIDSTLTELEDYYGFERAAGIYRRSGAAVAGLSKLIAAHGIACGFRPRNTLYLATNQEGARELLEERQLRRRA
GLPGVYLEHPDLFTQFELDRDGAIYSPGSAEADPLLLTWALIEMSVRRGARLVNASVTALHSEGDHVTAETDEGHVIEAR
HAVLATGYSMPGLDMPKLHRTSSSWALATVAQDPANFWRDRALIWEDSHPYLYMRTTEDNRIVAGGEDDGTADPEARDLK
LPAKIMAIREKIKRLWPKADTRVDHAWCGTFGETADGLPLIGPVPKMPHVFAAYGYGGNGITFSYLAAQMIGAMLAGMHR
DWFEDFALDRDGPGLRRFGEDRLGAGSELR
>Mature_429_residues
ASDLALNEADHDQMVSGRSLWGKSGIRPEWRPIEQSFSTDIIVIGGGITGALVGEHLTARGFSVVIIDREEPGFGSTAAS
TAMLQWEIDSTLTELEDYYGFERAAGIYRRSGAAVAGLSKLIAAHGIACGFRPRNTLYLATNQEGARELLEERQLRRRAG
LPGVYLEHPDLFTQFELDRDGAIYSPGSAEADPLLLTWALIEMSVRRGARLVNASVTALHSEGDHVTAETDEGHVIEARH
AVLATGYSMPGLDMPKLHRTSSSWALATVAQDPANFWRDRALIWEDSHPYLYMRTTEDNRIVAGGEDDGTADPEARDLKL
PAKIMAIREKIKRLWPKADTRVDHAWCGTFGETADGLPLIGPVPKMPHVFAAYGYGGNGITFSYLAAQMIGAMLAGMHRD
WFEDFALDRDGPGLRRFGEDRLGAGSELR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=385, Percent_Identity=24.1558441558442, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47153; Mature: 47021

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDLALNEADHDQMVSGRSLWGKSGIRPEWRPIEQSFSTDIIVIGGGITGALVGEHLTA
CCCCCCCCCCCHHHHHCCCHHCCCCCCCCCCCHHHHCCCCCEEEECCCCHHHHHHHHHHC
RGFSVVIIDREEPGFGSTAASTAMLQWEIDSTLTELEDYYGFERAAGIYRRSGAAVAGLS
CCEEEEEEECCCCCCCCCHHCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHH
KLIAAHGIACGFRPRNTLYLATNQEGARELLEERQLRRRAGLPGVYLEHPDLFTQFELDR
HHHHHHCCEECCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECC
DGAIYSPGSAEADPLLLTWALIEMSVRRGARLVNASVTALHSEGDHVTAETDEGHVIEAR
CCCEECCCCCCCCCHHHHHHHHHHHHHHCCEEEECEEEEEECCCCEEEEECCCCCEEEEC
HAVLATGYSMPGLDMPKLHRTSSSWALATVAQDPANFWRDRALIWEDSHPYLYMRTTEDN
CEEEEECCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHCCEEEEECCCCEEEEEECCCC
RIVAGGEDDGTADPEARDLKLPAKIMAIREKIKRLWPKADTRVDHAWCGTFGETADGLPL
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCC
IGPVPKMPHVFAAYGYGGNGITFSYLAAQMIGAMLAGMHRDWFEDFALDRDGPGLRRFGE
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCH
DRLGAGSELR
HHCCCCCCCC
>Mature Secondary Structure 
ASDLALNEADHDQMVSGRSLWGKSGIRPEWRPIEQSFSTDIIVIGGGITGALVGEHLTA
CCCCCCCCCCHHHHHCCCHHCCCCCCCCCCCHHHHCCCCCEEEECCCCHHHHHHHHHHC
RGFSVVIIDREEPGFGSTAASTAMLQWEIDSTLTELEDYYGFERAAGIYRRSGAAVAGLS
CCEEEEEEECCCCCCCCCHHCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHH
KLIAAHGIACGFRPRNTLYLATNQEGARELLEERQLRRRAGLPGVYLEHPDLFTQFELDR
HHHHHHCCEECCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECC
DGAIYSPGSAEADPLLLTWALIEMSVRRGARLVNASVTALHSEGDHVTAETDEGHVIEAR
CCCEECCCCCCCCCHHHHHHHHHHHHHHCCEEEECEEEEEECCCCEEEEECCCCCEEEEC
HAVLATGYSMPGLDMPKLHRTSSSWALATVAQDPANFWRDRALIWEDSHPYLYMRTTEDN
CEEEEECCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHCCEEEEECCCCEEEEEECCCC
RIVAGGEDDGTADPEARDLKLPAKIMAIREKIKRLWPKADTRVDHAWCGTFGETADGLPL
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCC
IGPVPKMPHVFAAYGYGGNGITFSYLAAQMIGAMLAGMHRDWFEDFALDRDGPGLRRFGE
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCH
DRLGAGSELR
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]