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The map label for this gene is emrB [H]

Identifier: 209548438

GI number: 209548438

Start: 854119

End: 855729

Strand: Direct

Name: emrB [H]

Synonym: Rleg2_0835

Alternate gene names: 209548438

Gene position: 854119-855729 (Clockwise)

Preceding gene: 209548437

Following gene: 209548439

Centisome position: 18.82

GC content: 62.51

Gene sequence:

>1611_bases
ATGGCCACTATTCAGACCGCCGCAAACAGCAATTCGCTCACACGCCCCGCCGCGGCGGCGCCCGCCGCCATCAACCCGGT
CCGCATGTGGATGGCCGTCGTCGGCTCCACCCTCGGCGCCTTCATGGCGGTCTTGAACATCCAGATCGTCAATGCCTCGC
TGGCCGATATCCAGGGCGCCATCGGCGCCGGCACGGATGACGGCGGCTGGATCTCGACCTCCTATCTGATCGCCGAGATC
GTCGTCATTCCCTTGAGCGGCTGGCTGACGCGGGTCTTCTCGCTGCGCAACTACCTGCTCGTCAACGCCATCCTCTTTCT
GATCTTCTCCGTCGCCTGCGCCTTTGCGGCCAATCTGCAGCAGATGATCGTCCTGCGCGCCATTCAGGGTTTTTCCGGCG
GCGTGCTGATCCCGATGGCCTTCACCATCATCATCACGCTGCTGCCCAAGGCGAGGCAGCCGATCGGGCTGGCGCTCTTT
GCCCTTTCGGCCACTTTCGCACCGGCGATCGGCCCGACCATCGGCGGTTATCTCACCGAGAACTGGGGCTGGGAATATAT
CTTCTATGTCAACCTGGTGCCCGGCGCGCTGATGATCGGCCTGCTCTTTGCCTCGCTCGACCGGGCGCCGATGAACCTGA
AGCTGCTCGCCAAGGGCGACTGGCCCGGCATCGTCACCATGGCGATCGGGTTGGCGGGCCTGCAGACGGTGCTGGAAGAG
GGCAACAAGGAAGACTGGTTCGGCTCCGATTTCATCCTGCGCCTGACTGTTATCGCTGCGGTTTCGCTGACCCTGTTCAT
TGTCATCGAGCTGAAGACGGCCCATCCGCTCCTGAACCTGCGTCTGCTGGTGCGCCGCAATTTCGGCTTCGGCATCGTTG
CCAACTTCCTGCTCGGCATCGCGCTTTACGGCTCGGTCTTCGTGCTGCCGATCTATCTCACCCGTATCCAGGGTTATAAT
TCCGAGCAGATCGGCATGGTGCTCGCGTGGACCGGCATCCCGCAGCTGCTGCTGATCCCACTGGTGCCGCGGCTGATGAA
GCGTTTCGACCTCCGCCTGCTGATCGTCGTCGGCTTTGCCCTTTTTGCCGCCTCGAATTTCATGAACGTGCATATGACCG
GCGATTATGCCAGCGATCAGCTGTTCTGGCCGAACATCGTGCGCGCCATCGGCCAGGCGCTGGTCTTCACGCCCCTTTCG
GCGATCGCCACATCCGGCATCGAGCCGGAGAATGCCGGTTCGGCGTCGTCGCTGTTCAACATGATGCGCAATCTCGGCGG
CGCGGTCGGCATCGCCTCGCTGCAGACCTTCCTGTCGAAGCGCGAGCAGTTCCACTCGAATATCCTGACCAACTCGGTCT
CGGTCTTCGAGGAAGCCACGCGCGATCGCATTGCCAGGTTGACGGCCTATTTCATGAGCCACGGCGTCAGCGATCAGGCG
CTCGCCGGCCACAAGGCCGTCGTCGCCATTGCGCTCAAAATACGCAAGCAGGCCAATATCATGGCCTTCAGCGACACCTT
CTTCCTGCTCGGCGTCGCCCTGGTCGTCGCCCTGCTGGCAAGCCTGCTTCTCAGCAAACCCGGTCAACTCTCCGGCGGCG
GCGCTCACTAG

Upstream 100 bases:

>100_bases
AGAATTATCCCGGCACGCCGGATAGCCCTTCCGCCTTTTCCCAAATTCGCCCGAACCCCGTCATGAAAGGTTTTCCGTGA
GAACCGGAAAGAGATCAGCC

Downstream 100 bases:

>100_bases
AGCTTGAAGGAGGAGAACGCCATGACCTCAGCGATCCATATCCCCGAAACCGGCCGGTTTCAGCCAAAGGCGGCTGGCAA
TGCCGTCAGCGCCGGCGCAC

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MATIQTAANSNSLTRPAAAAPAAINPVRMWMAVVGSTLGAFMAVLNIQIVNASLADIQGAIGAGTDDGGWISTSYLIAEI
VVIPLSGWLTRVFSLRNYLLVNAILFLIFSVACAFAANLQQMIVLRAIQGFSGGVLIPMAFTIIITLLPKARQPIGLALF
ALSATFAPAIGPTIGGYLTENWGWEYIFYVNLVPGALMIGLLFASLDRAPMNLKLLAKGDWPGIVTMAIGLAGLQTVLEE
GNKEDWFGSDFILRLTVIAAVSLTLFIVIELKTAHPLLNLRLLVRRNFGFGIVANFLLGIALYGSVFVLPIYLTRIQGYN
SEQIGMVLAWTGIPQLLLIPLVPRLMKRFDLRLLIVVGFALFAASNFMNVHMTGDYASDQLFWPNIVRAIGQALVFTPLS
AIATSGIEPENAGSASSLFNMMRNLGGAVGIASLQTFLSKREQFHSNILTNSVSVFEEATRDRIARLTAYFMSHGVSDQA
LAGHKAVVAIALKIRKQANIMAFSDTFFLLGVALVVALLASLLLSKPGQLSGGGAH

Sequences:

>Translated_536_residues
MATIQTAANSNSLTRPAAAAPAAINPVRMWMAVVGSTLGAFMAVLNIQIVNASLADIQGAIGAGTDDGGWISTSYLIAEI
VVIPLSGWLTRVFSLRNYLLVNAILFLIFSVACAFAANLQQMIVLRAIQGFSGGVLIPMAFTIIITLLPKARQPIGLALF
ALSATFAPAIGPTIGGYLTENWGWEYIFYVNLVPGALMIGLLFASLDRAPMNLKLLAKGDWPGIVTMAIGLAGLQTVLEE
GNKEDWFGSDFILRLTVIAAVSLTLFIVIELKTAHPLLNLRLLVRRNFGFGIVANFLLGIALYGSVFVLPIYLTRIQGYN
SEQIGMVLAWTGIPQLLLIPLVPRLMKRFDLRLLIVVGFALFAASNFMNVHMTGDYASDQLFWPNIVRAIGQALVFTPLS
AIATSGIEPENAGSASSLFNMMRNLGGAVGIASLQTFLSKREQFHSNILTNSVSVFEEATRDRIARLTAYFMSHGVSDQA
LAGHKAVVAIALKIRKQANIMAFSDTFFLLGVALVVALLASLLLSKPGQLSGGGAH
>Mature_535_residues
ATIQTAANSNSLTRPAAAAPAAINPVRMWMAVVGSTLGAFMAVLNIQIVNASLADIQGAIGAGTDDGGWISTSYLIAEIV
VIPLSGWLTRVFSLRNYLLVNAILFLIFSVACAFAANLQQMIVLRAIQGFSGGVLIPMAFTIIITLLPKARQPIGLALFA
LSATFAPAIGPTIGGYLTENWGWEYIFYVNLVPGALMIGLLFASLDRAPMNLKLLAKGDWPGIVTMAIGLAGLQTVLEEG
NKEDWFGSDFILRLTVIAAVSLTLFIVIELKTAHPLLNLRLLVRRNFGFGIVANFLLGIALYGSVFVLPIYLTRIQGYNS
EQIGMVLAWTGIPQLLLIPLVPRLMKRFDLRLLIVVGFALFAASNFMNVHMTGDYASDQLFWPNIVRAIGQALVFTPLSA
IATSGIEPENAGSASSLFNMMRNLGGAVGIASLQTFLSKREQFHSNILTNSVSVFEEATRDRIARLTAYFMSHGVSDQAL
AGHKAVVAIALKIRKQANIMAFSDTFFLLGVALVVALLASLLLSKPGQLSGGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=494, Percent_Identity=30.3643724696356, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1788710, Length=459, Percent_Identity=30.9368191721133, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1790195, Length=429, Percent_Identity=26.8065268065268, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081983, Length=421, Percent_Identity=26.1282660332542, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788392, Length=401, Percent_Identity=24.9376558603491, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6325455, Length=143, Percent_Identity=29.3706293706294, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=384, Percent_Identity=25, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=204, Percent_Identity=23.0392156862745, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57458; Mature: 57327

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIQTAANSNSLTRPAAAAPAAINPVRMWMAVVGSTLGAFMAVLNIQIVNASLADIQGA
CCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHH
IGAGTDDGGWISTSYLIAEIVVIPLSGWLTRVFSLRNYLLVNAILFLIFSVACAFAANLQ
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMIVLRAIQGFSGGVLIPMAFTIIITLLPKARQPIGLALFALSATFAPAIGPTIGGYLTE
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEC
NWGWEYIFYVNLVPGALMIGLLFASLDRAPMNLKLLAKGDWPGIVTMAIGLAGLQTVLEE
CCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
GNKEDWFGSDFILRLTVIAAVSLTLFIVIELKTAHPLLNLRLLVRRNFGFGIVANFLLGI
CCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHEECCCCCHHHHHHHHHHH
ALYGSVFVLPIYLTRIQGYNSEQIGMVLAWTGIPQLLLIPLVPRLMKRFDLRLLIVVGFA
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHH
LFAASNFMNVHMTGDYASDQLFWPNIVRAIGQALVFTPLSAIATSGIEPENAGSASSLFN
HHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
MMRNLGGAVGIASLQTFLSKREQFHSNILTNSVSVFEEATRDRIARLTAYFMSHGVSDQA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LAGHKAVVAIALKIRKQANIMAFSDTFFLLGVALVVALLASLLLSKPGQLSGGGAH
HCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ATIQTAANSNSLTRPAAAAPAAINPVRMWMAVVGSTLGAFMAVLNIQIVNASLADIQGA
CCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHH
IGAGTDDGGWISTSYLIAEIVVIPLSGWLTRVFSLRNYLLVNAILFLIFSVACAFAANLQ
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMIVLRAIQGFSGGVLIPMAFTIIITLLPKARQPIGLALFALSATFAPAIGPTIGGYLTE
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEC
NWGWEYIFYVNLVPGALMIGLLFASLDRAPMNLKLLAKGDWPGIVTMAIGLAGLQTVLEE
CCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
GNKEDWFGSDFILRLTVIAAVSLTLFIVIELKTAHPLLNLRLLVRRNFGFGIVANFLLGI
CCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHEECCCCCHHHHHHHHHHH
ALYGSVFVLPIYLTRIQGYNSEQIGMVLAWTGIPQLLLIPLVPRLMKRFDLRLLIVVGFA
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHH
LFAASNFMNVHMTGDYASDQLFWPNIVRAIGQALVFTPLSAIATSGIEPENAGSASSLFN
HHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
MMRNLGGAVGIASLQTFLSKREQFHSNILTNSVSVFEEATRDRIARLTAYFMSHGVSDQA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LAGHKAVVAIALKIRKQANIMAFSDTFFLLGVALVVALLASLLLSKPGQLSGGGAH
HCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]