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The map label for this gene is emrA [H]

Identifier: 209548437

GI number: 209548437

Start: 852853

End: 854043

Strand: Direct

Name: emrA [H]

Synonym: Rleg2_0834

Alternate gene names: 209548437

Gene position: 852853-854043 (Clockwise)

Preceding gene: 209548435

Following gene: 209548438

Centisome position: 18.79

GC content: 65.58

Gene sequence:

>1191_bases
ATGGTTGAGTTGCCCCGCAAGGATGTTTTCGACACGGCCCGGCAGGCCGATGGCGCGCCTGCGACCGAAGCGGCTGTCGT
CGAAGCCCCAGCCGCGGCCGTGCCGAAGAAAACCGGCCGCAAGATTGTCAAGCGCGCGGTCATCGCCGCAGCCCTGCTTG
CCGGCGTCGGTTTATCAGGCGATTTCGGTTACCGCTATTGGACGGTCGGCCGCTTCATCGAATCCACCGACGATGCCTAT
GTGAAGGCCGATTACACCACCGTCGCCCCGAAGGTTGCCGGCTATATCAGGCAGGTGCTGGTCAACGACAACGACCCGGT
CAAGTCAGGCCAGGTTCTCGCTCGCATCGACGACCGCGACTTCCAGGCCGCATTGTCGCAGGCGAGGGCCGCCGTGAAGG
CGGCCGATGCCGCGATCGCCAATATCGACGCCCAGATCGCCTTGCAGCAGTCGGTGATCGGCCAGGCCAAGGCCACGATC
GATGCCTCGCAGGCCTCGCTCGATTTTGCCGTTTCGGATGCTGCCCGCTCGGCCCGGCTGATCACCAGCGGCGCCGGCAC
GCAATCGCGCGCCGAACAGAGCCAGTCGGCCCGCGACCAGGCCGCCGCCGCCGTCGAGCGCGACCGGGCAGCCCTCGTCG
CGGCTGAGAACAAGGTGCCGGTCCTTGAAACGCAGCGCCAGCAGGCAATTGCCGAGCGCGATCGGGCGGCAGCCGCCGCC
CAGCAGGCCGAACTCAACCTGTCCTATACTGATATCGTCGCCGCCGTCGACGGCACGGTCGGCGCCCGTTCGATCCGCGT
CGGCCAGTATGTCACCTCGGGCACGCAGCTGATGGCCGTCGTGCCGCTGCATGCCGTCTATGTCGTCGCCAATTTCAAGG
AGACGCAGCTGACCCATGTCCGCCCCGGCCAGCCGGTCGAGATCAAGGTGGACAGCTTTCCCGACATGGCGATCAAAGGC
CATGTCGACAGCGTTTCACCGGCGAGCGGCCTCGAATTCTCGCTGCTGCCACCTGACAACGCCACCGGCAATTTCACCAA
GATCGTCCAGCGCATCCCGGTCAAGATCGTCATCGACGACGAGGCGCTGAGCGGCCTGTTGCGCTCGGGCATGTCGGTCG
AGCCGGAGATCGATACCAAGGCTGCAGAGACCTCTGTGGCCGAGGAAGAATTATCCCGGCACGCCGGATAG

Upstream 100 bases:

>100_bases
TCCCGATATAAGCAGATTATCACAATGAACGGCAGGGTGCATTGTCCCTTCAATCGAACAGGGCCTCTGAAACGGAGGCC
GGCATTGAAGGAGAAACACA

Downstream 100 bases:

>100_bases
CCCTTCCGCCTTTTCCCAAATTCGCCCGAACCCCGTCATGAAAGGTTTTCCGTGAGAACCGGAAAGAGATCAGCCATGGC
CACTATTCAGACCGCCGCAA

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MVELPRKDVFDTARQADGAPATEAAVVEAPAAAVPKKTGRKIVKRAVIAAALLAGVGLSGDFGYRYWTVGRFIESTDDAY
VKADYTTVAPKVAGYIRQVLVNDNDPVKSGQVLARIDDRDFQAALSQARAAVKAADAAIANIDAQIALQQSVIGQAKATI
DASQASLDFAVSDAARSARLITSGAGTQSRAEQSQSARDQAAAAVERDRAALVAAENKVPVLETQRQQAIAERDRAAAAA
QQAELNLSYTDIVAAVDGTVGARSIRVGQYVTSGTQLMAVVPLHAVYVVANFKETQLTHVRPGQPVEIKVDSFPDMAIKG
HVDSVSPASGLEFSLLPPDNATGNFTKIVQRIPVKIVIDDEALSGLLRSGMSVEPEIDTKAAETSVAEEELSRHAG

Sequences:

>Translated_396_residues
MVELPRKDVFDTARQADGAPATEAAVVEAPAAAVPKKTGRKIVKRAVIAAALLAGVGLSGDFGYRYWTVGRFIESTDDAY
VKADYTTVAPKVAGYIRQVLVNDNDPVKSGQVLARIDDRDFQAALSQARAAVKAADAAIANIDAQIALQQSVIGQAKATI
DASQASLDFAVSDAARSARLITSGAGTQSRAEQSQSARDQAAAAVERDRAALVAAENKVPVLETQRQQAIAERDRAAAAA
QQAELNLSYTDIVAAVDGTVGARSIRVGQYVTSGTQLMAVVPLHAVYVVANFKETQLTHVRPGQPVEIKVDSFPDMAIKG
HVDSVSPASGLEFSLLPPDNATGNFTKIVQRIPVKIVIDDEALSGLLRSGMSVEPEIDTKAAETSVAEEELSRHAG
>Mature_396_residues
MVELPRKDVFDTARQADGAPATEAAVVEAPAAAVPKKTGRKIVKRAVIAAALLAGVGLSGDFGYRYWTVGRFIESTDDAY
VKADYTTVAPKVAGYIRQVLVNDNDPVKSGQVLARIDDRDFQAALSQARAAVKAADAAIANIDAQIALQQSVIGQAKATI
DASQASLDFAVSDAARSARLITSGAGTQSRAEQSQSARDQAAAAVERDRAALVAAENKVPVLETQRQQAIAERDRAAAAA
QQAELNLSYTDIVAAVDGTVGARSIRVGQYVTSGTQLMAVVPLHAVYVVANFKETQLTHVRPGQPVEIKVDSFPDMAIKG
HVDSVSPASGLEFSLLPPDNATGNFTKIVQRIPVKIVIDDEALSGLLRSGMSVEPEIDTKAAETSVAEEELSRHAG

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=336, Percent_Identity=35.1190476190476, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1789041, Length=323, Percent_Identity=34.984520123839, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790519, Length=300, Percent_Identity=31, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081951, Length=301, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789637, Length=330, Percent_Identity=28.7878787878788, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787013, Length=324, Percent_Identity=29.6296296296296, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789900, Length=332, Percent_Identity=25.3012048192771, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41696; Mature: 41696

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVELPRKDVFDTARQADGAPATEAAVVEAPAAAVPKKTGRKIVKRAVIAAALLAGVGLSG
CCCCCCHHHHHHHHHCCCCCCCCHHEEECCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
DFGYRYWTVGRFIESTDDAYVKADYTTVAPKVAGYIRQVLVNDNDPVKSGQVLARIDDRD
CCCCEEEEHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHH
FQAALSQARAAVKAADAAIANIDAQIALQQSVIGQAKATIDASQASLDFAVSDAARSARL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCEE
ITSGAGTQSRAEQSQSARDQAAAAVERDRAALVAAENKVPVLETQRQQAIAERDRAAAAA
EECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
QQAELNLSYTDIVAAVDGTVGARSIRVGQYVTSGTQLMAVVPLHAVYVVANFKETQLTHV
HHHHCCCCHHHHHHHHCCCCCCCEEEECCEECCCCEEEEEHHHEEEEEEECCCCCEEEEC
RPGQPVEIKVDSFPDMAIKGHVDSVSPASGLEFSLLPPDNATGNFTKIVQRIPVKIVIDD
CCCCCEEEEECCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECH
EALSGLLRSGMSVEPEIDTKAAETSVAEEELSRHAG
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVELPRKDVFDTARQADGAPATEAAVVEAPAAAVPKKTGRKIVKRAVIAAALLAGVGLSG
CCCCCCHHHHHHHHHCCCCCCCCHHEEECCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
DFGYRYWTVGRFIESTDDAYVKADYTTVAPKVAGYIRQVLVNDNDPVKSGQVLARIDDRD
CCCCEEEEHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHH
FQAALSQARAAVKAADAAIANIDAQIALQQSVIGQAKATIDASQASLDFAVSDAARSARL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCEE
ITSGAGTQSRAEQSQSARDQAAAAVERDRAALVAAENKVPVLETQRQQAIAERDRAAAAA
EECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
QQAELNLSYTDIVAAVDGTVGARSIRVGQYVTSGTQLMAVVPLHAVYVVANFKETQLTHV
HHHHCCCCHHHHHHHHCCCCCCCEEEECCEECCCCEEEEEHHHEEEEEEECCCCCEEEEC
RPGQPVEIKVDSFPDMAIKGHVDSVSPASGLEFSLLPPDNATGNFTKIVQRIPVKIVIDD
CCCCCEEEEECCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECH
EALSGLLRSGMSVEPEIDTKAAETSVAEEELSRHAG
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]