| Definition | Rhizobium leguminosarum bv. trifolii WSM2304 chromosome, complete genome. |
|---|---|
| Accession | NC_011369 |
| Length | 4,537,948 |
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The map label for this gene is ykvO [H]
Identifier: 209548285
GI number: 209548285
Start: 709803
End: 710570
Strand: Reverse
Name: ykvO [H]
Synonym: Rleg2_0680
Alternate gene names: 209548285
Gene position: 710570-709803 (Counterclockwise)
Preceding gene: 209548287
Following gene: 209548281
Centisome position: 15.66
GC content: 65.36
Gene sequence:
>768_bases ATGAGATTTGAAGGCAAGGTGGTCGCCATTACCGGCGGTGGATCCGGCATCGGCCGGGAAGCGGCAGCCCGTTTCGCGGC GGAAGGCGCAAAGGTCGCGATCAACGGACGCGATCGCGAAAAGCTCGAGGCGGCGGCCCGGGAGATCGATCCGAGCGGCG ACAATGTCGTCATTTCGGCGGGCGATATCGCCAGGCCGGAAACCGGCGCGGCCCTCGTCGACGCCGCCCTCTCCCATTTC GGCAGGCTCGATGTGCTCGTCAACAATGCCGGCGTCTTCAATCCGAAGCCGTTTCTGGAGCTGACCGAGGGCGATTACGA CTGGTATCTCGACACCATCCTCAAGGGCAAATTCTTCACCGCCCAGGCCGCGGCCAAGGCGATGAAGGAGACGGGCGGCG CCATCGTCCAGACCGGCTCGATGTGGGCGATCCAGGCGATCGGCGCTACCCCGTCTGCGGCCTATTCGGCAGCCAATGCC GGCGTCCATGCCATGGTCCGCAACCTCGCGATCGAGCTTGCGCCCTTTAATATCCGCATCAACGCCGTGGCGCCGGCCGT CGTCGAGACACCCGTCTACAACACCTTCCTCTCCGACGATCAGGTCAAGCAAGTGCTTCCGGGCTTCAACGCCTTCCACC CGCTCGGCCGCAACGGCCAGCCGCGTGATGTCGCCGAGGCGATCCTGTTCCTCGCCTCCGAACAAGCCTCGTGGATCACC GGCACGGTCCTGCCCGTCGACGGCGGCGTCACCGCCGGCCGGCAATAA
Upstream 100 bases:
>100_bases ATCTAATGTTAGATAGGGGTTGCGTTTACCCGAAGCAAGGACTATGTCTCCGTTGTCTATCTAACGTTAGATAGACAACG GAGACAAAACGGAGAGAACG
Downstream 100 bases:
>100_bases GGAGGGGCAAGACCGCTCAGCTGGAGCCGAAGATGCACGTTGCGATCACTACCCGCTAATGCGGCGTTGAGCTCGCGGCC ATGGGTCCTCGGGTCAAGCC
Product: short-chain dehydrogenase/reductase SDR
Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]
Alternate protein names: NA
Number of amino acids: Translated: 255; Mature: 255
Protein sequence:
>255_residues MRFEGKVVAITGGGSGIGREAAARFAAEGAKVAINGRDREKLEAAAREIDPSGDNVVISAGDIARPETGAALVDAALSHF GRLDVLVNNAGVFNPKPFLELTEGDYDWYLDTILKGKFFTAQAAAKAMKETGGAIVQTGSMWAIQAIGATPSAAYSAANA GVHAMVRNLAIELAPFNIRINAVAPAVVETPVYNTFLSDDQVKQVLPGFNAFHPLGRNGQPRDVAEAILFLASEQASWIT GTVLPVDGGVTAGRQ
Sequences:
>Translated_255_residues MRFEGKVVAITGGGSGIGREAAARFAAEGAKVAINGRDREKLEAAAREIDPSGDNVVISAGDIARPETGAALVDAALSHF GRLDVLVNNAGVFNPKPFLELTEGDYDWYLDTILKGKFFTAQAAAKAMKETGGAIVQTGSMWAIQAIGATPSAAYSAANA GVHAMVRNLAIELAPFNIRINAVAPAVVETPVYNTFLSDDQVKQVLPGFNAFHPLGRNGQPRDVAEAILFLASEQASWIT GTVLPVDGGVTAGRQ >Mature_255_residues MRFEGKVVAITGGGSGIGREAAARFAAEGAKVAINGRDREKLEAAAREIDPSGDNVVISAGDIARPETGAALVDAALSHF GRLDVLVNNAGVFNPKPFLELTEGDYDWYLDTILKGKFFTAQAAAKAMKETGGAIVQTGSMWAIQAIGATPSAAYSAANA GVHAMVRNLAIELAPFNIRINAVAPAVVETPVYNTFLSDDQVKQVLPGFNAFHPLGRNGQPRDVAEAILFLASEQASWIT GTVLPVDGGVTAGRQ
Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI15277342, Length=260, Percent_Identity=32.3076923076923, Blast_Score=122, Evalue=3e-28, Organism=Homo sapiens, GI40254992, Length=246, Percent_Identity=34.9593495934959, Blast_Score=119, Evalue=3e-27, Organism=Homo sapiens, GI7705925, Length=249, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=1e-26, Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=32, Blast_Score=111, Evalue=7e-25, Organism=Homo sapiens, GI19923817, Length=256, Percent_Identity=34.765625, Blast_Score=109, Evalue=2e-24, Organism=Homo sapiens, GI10190704, Length=246, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=3e-22, Organism=Homo sapiens, GI5031737, Length=245, Percent_Identity=30.2040816326531, Blast_Score=102, Evalue=4e-22, Organism=Homo sapiens, GI59889578, Length=232, Percent_Identity=31.4655172413793, Blast_Score=101, Evalue=6e-22, Organism=Homo sapiens, GI4504505, Length=221, Percent_Identity=33.4841628959276, Blast_Score=100, Evalue=2e-21, Organism=Homo sapiens, GI126723750, Length=251, Percent_Identity=26.6932270916335, Blast_Score=85, Evalue=7e-17, Organism=Homo sapiens, GI4758504, Length=260, Percent_Identity=29.6153846153846, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI33667109, Length=251, Percent_Identity=29.0836653386454, Blast_Score=82, Evalue=6e-16, Organism=Homo sapiens, GI31542939, Length=211, Percent_Identity=28.9099526066351, Blast_Score=79, Evalue=4e-15, Organism=Homo sapiens, GI66933014, Length=250, Percent_Identity=26.8, Blast_Score=78, Evalue=9e-15, Organism=Homo sapiens, GI4503301, Length=251, Percent_Identity=29.8804780876494, Blast_Score=76, Evalue=2e-14, Organism=Homo sapiens, GI126723191, Length=187, Percent_Identity=31.0160427807487, Blast_Score=75, Evalue=6e-14, Organism=Homo sapiens, GI209862901, Length=220, Percent_Identity=28.1818181818182, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI19923983, Length=220, Percent_Identity=28.1818181818182, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI109715829, Length=250, Percent_Identity=26.8, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI20149619, Length=198, Percent_Identity=31.3131313131313, Blast_Score=68, Evalue=6e-12, Organism=Homo sapiens, GI83715985, Length=188, Percent_Identity=30.8510638297872, Blast_Score=68, Evalue=7e-12, Organism=Homo sapiens, GI4503817, Length=193, Percent_Identity=27.4611398963731, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI186928839, Length=202, Percent_Identity=31.1881188118812, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI224922801, Length=173, Percent_Identity=30.0578034682081, Blast_Score=65, Evalue=6e-11, Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=33.7349397590361, Blast_Score=135, Evalue=3e-33, Organism=Escherichia coli, GI1787335, Length=251, Percent_Identity=34.2629482071713, Blast_Score=126, Evalue=1e-30, Organism=Escherichia coli, GI1789208, Length=250, Percent_Identity=32.8, Blast_Score=120, Evalue=1e-28, Organism=Escherichia coli, GI1788459, Length=249, Percent_Identity=32.9317269076305, Blast_Score=113, Evalue=1e-26, Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=31.496062992126, Blast_Score=112, Evalue=2e-26, Organism=Escherichia coli, GI1790717, Length=251, Percent_Identity=29.0836653386454, Blast_Score=104, Evalue=6e-24, Organism=Escherichia coli, GI1786812, Length=262, Percent_Identity=34.3511450381679, Blast_Score=102, Evalue=2e-23, Organism=Escherichia coli, GI87082160, Length=257, Percent_Identity=30.3501945525292, Blast_Score=100, Evalue=9e-23, Organism=Escherichia coli, GI1789378, Length=257, Percent_Identity=31.1284046692607, Blast_Score=96, Evalue=3e-21, Organism=Escherichia coli, GI2367175, Length=252, Percent_Identity=30.5555555555556, Blast_Score=92, Evalue=3e-20, Organism=Escherichia coli, GI1789057, Length=258, Percent_Identity=29.0697674418605, Blast_Score=91, Evalue=8e-20, Organism=Escherichia coli, GI2367365, Length=246, Percent_Identity=30.8943089430894, Blast_Score=83, Evalue=2e-17, Organism=Escherichia coli, GI1787820, Length=235, Percent_Identity=26.3829787234043, Blast_Score=68, Evalue=7e-13, Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=33.4600760456274, Blast_Score=141, Evalue=3e-34, Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=34.6613545816733, Blast_Score=133, Evalue=9e-32, Organism=Caenorhabditis elegans, GI17562904, Length=264, Percent_Identity=36.3636363636364, Blast_Score=130, Evalue=8e-31, Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=34, Blast_Score=128, Evalue=2e-30, Organism=Caenorhabditis elegans, GI17544670, Length=261, Percent_Identity=32.9501915708812, Blast_Score=128, Evalue=3e-30, Organism=Caenorhabditis elegans, GI72000259, Length=265, Percent_Identity=34.3396226415094, Blast_Score=127, Evalue=4e-30, Organism=Caenorhabditis elegans, GI17562906, Length=276, Percent_Identity=33.695652173913, Blast_Score=123, Evalue=1e-28, Organism=Caenorhabditis elegans, GI17559104, Length=261, Percent_Identity=35.2490421455939, Blast_Score=122, Evalue=2e-28, Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=33.2075471698113, Blast_Score=120, Evalue=6e-28, Organism=Caenorhabditis elegans, GI17531453, Length=261, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-27, Organism=Caenorhabditis elegans, GI17562990, Length=265, Percent_Identity=30.9433962264151, Blast_Score=115, Evalue=2e-26, Organism=Caenorhabditis elegans, GI17561402, Length=257, Percent_Identity=34.2412451361868, Blast_Score=115, Evalue=2e-26, Organism=Caenorhabditis elegans, GI17563726, Length=268, Percent_Identity=30.9701492537313, Blast_Score=115, Evalue=3e-26, Organism=Caenorhabditis elegans, GI17560332, Length=268, Percent_Identity=33.2089552238806, Blast_Score=114, Evalue=5e-26, Organism=Caenorhabditis elegans, GI17560150, Length=265, Percent_Identity=30.5660377358491, Blast_Score=114, Evalue=6e-26, Organism=Caenorhabditis elegans, GI17551412, Length=263, Percent_Identity=34.2205323193916, Blast_Score=112, Evalue=1e-25, Organism=Caenorhabditis elegans, GI17562908, Length=263, Percent_Identity=32.6996197718631, Blast_Score=111, Evalue=3e-25, Organism=Caenorhabditis elegans, GI17565030, Length=272, Percent_Identity=33.0882352941176, Blast_Score=111, Evalue=3e-25, Organism=Caenorhabditis elegans, GI17560220, Length=260, Percent_Identity=31.1538461538462, Blast_Score=110, Evalue=8e-25, Organism=Caenorhabditis elegans, GI17538480, Length=263, Percent_Identity=32.6996197718631, Blast_Score=110, Evalue=9e-25, Organism=Caenorhabditis elegans, GI17555706, Length=250, Percent_Identity=31.2, Blast_Score=109, Evalue=1e-24, Organism=Caenorhabditis elegans, GI17536651, Length=252, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=5e-24, Organism=Caenorhabditis elegans, GI17536025, Length=251, Percent_Identity=33.0677290836653, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI17560676, Length=255, Percent_Identity=29.4117647058824, Blast_Score=103, Evalue=9e-23, Organism=Caenorhabditis elegans, GI115534694, Length=250, Percent_Identity=31.6, Blast_Score=103, Evalue=9e-23, Organism=Caenorhabditis elegans, GI17538486, Length=263, Percent_Identity=31.5589353612167, Blast_Score=102, Evalue=1e-22, Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=29.4354838709677, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17568967, Length=195, Percent_Identity=32.8205128205128, Blast_Score=95, Evalue=4e-20, Organism=Caenorhabditis elegans, GI17561272, Length=251, Percent_Identity=32.6693227091633, Blast_Score=82, Evalue=3e-16, Organism=Caenorhabditis elegans, GI17508651, Length=254, Percent_Identity=27.5590551181102, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17538182, Length=264, Percent_Identity=28.030303030303, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17508895, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI71997099, Length=224, Percent_Identity=26.3392857142857, Blast_Score=64, Evalue=7e-11, Organism=Saccharomyces cerevisiae, GI6324126, Length=252, Percent_Identity=32.5396825396825, Blast_Score=115, Evalue=8e-27, Organism=Saccharomyces cerevisiae, GI6322861, Length=244, Percent_Identity=29.9180327868852, Blast_Score=94, Evalue=2e-20, Organism=Saccharomyces cerevisiae, GI6322067, Length=203, Percent_Identity=29.5566502463054, Blast_Score=70, Evalue=3e-13, Organism=Drosophila melanogaster, GI24644339, Length=251, Percent_Identity=37.4501992031873, Blast_Score=143, Evalue=9e-35, Organism=Drosophila melanogaster, GI23397609, Length=250, Percent_Identity=34.8, Blast_Score=142, Evalue=3e-34, Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=34.765625, Blast_Score=134, Evalue=6e-32, Organism=Drosophila melanogaster, GI21357041, Length=249, Percent_Identity=33.7349397590361, Blast_Score=124, Evalue=6e-29, Organism=Drosophila melanogaster, GI24644337, Length=253, Percent_Identity=35.5731225296443, Blast_Score=120, Evalue=9e-28, Organism=Drosophila melanogaster, GI24639444, Length=253, Percent_Identity=35.9683794466403, Blast_Score=115, Evalue=2e-26, Organism=Drosophila melanogaster, GI24643142, Length=247, Percent_Identity=33.1983805668016, Blast_Score=108, Evalue=4e-24, Organism=Drosophila melanogaster, GI21355319, Length=254, Percent_Identity=29.9212598425197, Blast_Score=103, Evalue=1e-22, Organism=Drosophila melanogaster, GI18859837, Length=195, Percent_Identity=33.8461538461538, Blast_Score=99, Evalue=4e-21, Organism=Drosophila melanogaster, GI17737361, Length=257, Percent_Identity=30.3501945525292, Blast_Score=76, Evalue=2e-14, Organism=Drosophila melanogaster, GI24580925, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=4e-13, Organism=Drosophila melanogaster, GI281364892, Length=194, Percent_Identity=28.8659793814433, Blast_Score=72, Evalue=5e-13, Organism=Drosophila melanogaster, GI24584752, Length=216, Percent_Identity=28.7037037037037, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI24584748, Length=205, Percent_Identity=30.7317073170732, Blast_Score=65, Evalue=5e-11, Organism=Drosophila melanogaster, GI21357673, Length=192, Percent_Identity=24.4791666666667, Blast_Score=65, Evalue=5e-11, Organism=Drosophila melanogaster, GI161076933, Length=205, Percent_Identity=30.7317073170732, Blast_Score=65, Evalue=5e-11, Organism=Drosophila melanogaster, GI21358495, Length=200, Percent_Identity=28.5, Blast_Score=64, Evalue=8e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.159 [C]
Molecular weight: Translated: 26634; Mature: 26634
Theoretical pI: Translated: 5.09; Mature: 5.09
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 1.6 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 1.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRFEGKVVAITGGGSGIGREAAARFAAEGAKVAINGRDREKLEAAAREIDPSGDNVVISA CCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEC GDIARPETGAALVDAALSHFGRLDVLVNNAGVFNPKPFLELTEGDYDWYLDTILKGKFFT CCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHCCCCCH AQAAAKAMKETGGAIVQTGSMWAIQAIGATPSAAYSAANAGVHAMVRNLAIELAPFNIRI HHHHHHHHHHCCCEEEEECCEEEEEEECCCCHHHHHHHCCCHHHHHHHHEEEEECEEEEE NAVAPAVVETPVYNTFLSDDQVKQVLPGFNAFHPLGRNGQPRDVAEAILFLASEQASWIT EEECCHHHCCCCHHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE GTVLPVDGGVTAGRQ EEEEEECCCCCCCCC >Mature Secondary Structure MRFEGKVVAITGGGSGIGREAAARFAAEGAKVAINGRDREKLEAAAREIDPSGDNVVISA CCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEC GDIARPETGAALVDAALSHFGRLDVLVNNAGVFNPKPFLELTEGDYDWYLDTILKGKFFT CCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHCCCCCH AQAAAKAMKETGGAIVQTGSMWAIQAIGATPSAAYSAANAGVHAMVRNLAIELAPFNIRI HHHHHHHHHHCCCEEEEECCEEEEEEECCCCHHHHHHHCCCHHHHHHHHEEEEECEEEEE NAVAPAVVETPVYNTFLSDDQVKQVLPGFNAFHPLGRNGQPRDVAEAILFLASEQASWIT EEECCHHHCCCCHHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE GTVLPVDGGVTAGRQ EEEEEECCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]
Metal ions: CaCl2; KCl; MnCl2; NaCl [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]
Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]
Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]
General reaction: Redox reaction [C]
Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]