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The map label for this gene is yfhQ [H]

Identifier: 209548258

GI number: 209548258

Start: 673402

End: 674499

Strand: Reverse

Name: yfhQ [H]

Synonym: Rleg2_0652

Alternate gene names: 209548258

Gene position: 674499-673402 (Counterclockwise)

Preceding gene: 209548263

Following gene: 209548257

Centisome position: 14.86

GC content: 65.3

Gene sequence:

>1098_bases
ATGACGATCACCACACCCGACACGCCCACCGCAAAGCCCCTGCTCGACTGGTACGACCGCCATCACCGCGATCTGCCCTG
GCGCATTTCGCCCGGCATGGCGGCCGGCGGCGTCAAACCCGATCCCTATCGCGTCTGGCTTTCCGAGGTGATGCTGCAGC
AGACGACGGTGCAGGCGGTCAAACCCTATTTCGCCAAGTTTCTGCAGCGCTGGCCTGAGGTGACCGATCTTGCCGCGGCC
GAAAACGATGCCGTGATGGCCGCCTGGGCCGGGCTCGGCTATTACGCGCGGGCCCGCAACCTGAAGAAATGCGCCGAAGC
GGTGGCGAAAGAGCATGGCGGGGTCTTTCCCGATACCGAGGAAGGGCTGAAGTCGCTCCCCGGCATCGGCGATTATACGG
CCGCCGCCGTCGCCGCCATCGCCTTCAACCGGCAGGCGGCTGTGATGGACGGCAATGTCGAGCGGGTGATCTCAAGGCTT
TATGCGATCGAAACGCCGCTTCCCGCGGGAAAGCCGCTGATGAAGGAAAAGGTGGCGCGGCTGACACCCGCGACGCGGCC
CGGCGATTTCGCCCAGGCGATGATGGATCTCGGGGCGACGATCTGCACGCCGAAGCGGCCGGCCTGTTCGCTCTGTCCCT
TCCGCGGCGCCTGCGCGGCGCTGAAACTTTCCGATCCCGAGCTATTTCCCGTCAAGGCGGCGAAGAAGGAGAAGCCGGTG
CGGCAGGGTGCGGCTTTCGTCGCGGTCACCGCAGACGGCGAGATCCTGCTCAGGCGGCGCGCCGAAAGCGGCCTGCTCGG
CGGCATGACCGAGGTGCCGACAACGGCCTGGACGGCGCGGCTCGACGGCGAAACCTCGGCTGCGGCCGCGCCCTTCGAGG
CGGCATGGCAGGCCTGCGGCACCGTCATCCATGTCTTCACCCATTTTGAACTCCGGCTGTTGATCTGGCGCGCGGCGATC
GCCGGCAAGGTGGATGACCGTCCGAATGACGGATGGTGGGAGCCGGTTACAAATCTTGAAGCGCAGGCCTTGCCGACCAT
CATGAAAAAAGCGATCGCAGCGGCTATTCCTCTCGCGTTCAAAACATCCAAGGGATGA

Upstream 100 bases:

>100_bases
TTGGACGCCCCTGCCAATCGCACAGGCAAATTGCAAAGCCGGCTTGATCGCGTGGCATTGCTTCGCCAAGTATAGGCACT
TCCCCCGATCCCGGCAAATC

Downstream 100 bases:

>100_bases
CCATGACTACGACTGAGATAAAACATATCGTTTTCGATATCGGCAAAGTCCTTATTCATTACGATCCGCATCTTGCCTTC
GCCCGCCTCATTCCCGACGA

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTITTPDTPTAKPLLDWYDRHHRDLPWRISPGMAAGGVKPDPYRVWLSEVMLQQTTVQAVKPYFAKFLQRWPEVTDLAAA
ENDAVMAAWAGLGYYARARNLKKCAEAVAKEHGGVFPDTEEGLKSLPGIGDYTAAAVAAIAFNRQAAVMDGNVERVISRL
YAIETPLPAGKPLMKEKVARLTPATRPGDFAQAMMDLGATICTPKRPACSLCPFRGACAALKLSDPELFPVKAAKKEKPV
RQGAAFVAVTADGEILLRRRAESGLLGGMTEVPTTAWTARLDGETSAAAAPFEAAWQACGTVIHVFTHFELRLLIWRAAI
AGKVDDRPNDGWWEPVTNLEAQALPTIMKKAIAAAIPLAFKTSKG

Sequences:

>Translated_365_residues
MTITTPDTPTAKPLLDWYDRHHRDLPWRISPGMAAGGVKPDPYRVWLSEVMLQQTTVQAVKPYFAKFLQRWPEVTDLAAA
ENDAVMAAWAGLGYYARARNLKKCAEAVAKEHGGVFPDTEEGLKSLPGIGDYTAAAVAAIAFNRQAAVMDGNVERVISRL
YAIETPLPAGKPLMKEKVARLTPATRPGDFAQAMMDLGATICTPKRPACSLCPFRGACAALKLSDPELFPVKAAKKEKPV
RQGAAFVAVTADGEILLRRRAESGLLGGMTEVPTTAWTARLDGETSAAAAPFEAAWQACGTVIHVFTHFELRLLIWRAAI
AGKVDDRPNDGWWEPVTNLEAQALPTIMKKAIAAAIPLAFKTSKG
>Mature_364_residues
TITTPDTPTAKPLLDWYDRHHRDLPWRISPGMAAGGVKPDPYRVWLSEVMLQQTTVQAVKPYFAKFLQRWPEVTDLAAAE
NDAVMAAWAGLGYYARARNLKKCAEAVAKEHGGVFPDTEEGLKSLPGIGDYTAAAVAAIAFNRQAAVMDGNVERVISRLY
AIETPLPAGKPLMKEKVARLTPATRPGDFAQAMMDLGATICTPKRPACSLCPFRGACAALKLSDPELFPVKAAKKEKPVR
QGAAFVAVTADGEILLRRRAESGLLGGMTEVPTTAWTARLDGETSAAAAPFEAAWQACGTVIHVFTHFELRLLIWRAAIA
GKVDDRPNDGWWEPVTNLEAQALPTIMKKAIAAAIPLAFKTSKG

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8- oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal effici

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HhH domain [H]

Homologues:

Organism=Homo sapiens, GI115298648, Length=401, Percent_Identity=35.6608478802993, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI115298654, Length=401, Percent_Identity=35.6608478802993, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI115298652, Length=401, Percent_Identity=35.6608478802993, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI115298650, Length=401, Percent_Identity=35.6608478802993, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI6912520, Length=401, Percent_Identity=35.6608478802993, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI190358497, Length=401, Percent_Identity=35.6608478802993, Blast_Score=207, Evalue=8e-54,
Organism=Escherichia coli, GI1789331, Length=324, Percent_Identity=41.6666666666667, Blast_Score=226, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 39490; Mature: 39359

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITTPDTPTAKPLLDWYDRHHRDLPWRISPGMAAGGVKPDPYRVWLSEVMLQQTTVQAV
CCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KPYFAKFLQRWPEVTDLAAAENDAVMAAWAGLGYYARARNLKKCAEAVAKEHGGVFPDTE
HHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EGLKSLPGIGDYTAAAVAAIAFNRQAAVMDGNVERVISRLYAIETPLPAGKPLMKEKVAR
HHHHHCCCCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LTPATRPGDFAQAMMDLGATICTPKRPACSLCPFRGACAALKLSDPELFPVKAAKKEKPV
HCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCHH
RQGAAFVAVTADGEILLRRRAESGLLGGMTEVPTTAWTARLDGETSAAAAPFEAAWQACG
HCCCEEEEEECCCHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCCCCCCCCHHHHHHHHH
TVIHVFTHFELRLLIWRAAIAGKVDDRPNDGWWEPVTNLEAQALPTIMKKAIAAAIPLAF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHE
KTSKG
ECCCC
>Mature Secondary Structure 
TITTPDTPTAKPLLDWYDRHHRDLPWRISPGMAAGGVKPDPYRVWLSEVMLQQTTVQAV
CCCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KPYFAKFLQRWPEVTDLAAAENDAVMAAWAGLGYYARARNLKKCAEAVAKEHGGVFPDTE
HHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EGLKSLPGIGDYTAAAVAAIAFNRQAAVMDGNVERVISRLYAIETPLPAGKPLMKEKVAR
HHHHHCCCCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LTPATRPGDFAQAMMDLGATICTPKRPACSLCPFRGACAALKLSDPELFPVKAAKKEKPV
HCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCHH
RQGAAFVAVTADGEILLRRRAESGLLGGMTEVPTTAWTARLDGETSAAAAPFEAAWQACG
HCCCEEEEEECCCHHHHHHHHHCCCCCCCHHCCCCCEEEEECCCCCCCCCCHHHHHHHHH
TVIHVFTHFELRLLIWRAAIAGKVDDRPNDGWWEPVTNLEAQALPTIMKKAIAAAIPLAF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHE
KTSKG
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]