Click here to switch to the map view.

The map label for this gene is ispH [H]

Identifier: 209548217

GI number: 209548217

Start: 635592

End: 636593

Strand: Direct

Name: ispH [H]

Synonym: Rleg2_0611

Alternate gene names: 209548217

Gene position: 635592-636593 (Clockwise)

Preceding gene: 209548216

Following gene: 209548218

Centisome position: 14.01

GC content: 64.17

Gene sequence:

>1002_bases
ATGAATATTGCGGCGAAACCTCCTTTGACGATCAGGCTCTGCGGGCCGCGCGGCTTCTGCGCCGGCGTCGACCGCGCCAT
CCAGATCGTCGTGCTGGCGCTGAAATCCTATGGCGCGCCTGTCTATGTGCGCCACGAGATCGTCCACAATCGCTATGTCG
TCGAGGGGCTGGAGGCCAAGGGCGCCGTCTTCGTCGAGGAGCTGGACGAAATCCCGGCCGAGCATCGCGCCCAGCCGGTG
GTCTTTTCAGCCCATGGCGTGCCGAAATCCGTGCCGGAAGATGCGGTAAGCCGCAATCTCTTTTACCTCGACGCCACCTG
CCCGCTGGTCTCCAAGGTCCACAAGCAGGCGATGCGCCACAATCGCCTTGGCCGCCATGTCGTCCTGATCGGCCATGCCG
GCCACCCCGAAGTGATCGGCACGATGGGCCAGCTGCCCGAGGGTTCGGTGTCGCTGATCGAGACGATCGAGGATGCCGAC
GCCTATATTCCTGTCGACGCCGACAATCTCGGTTACGTCACCCAGACGACGCTGTCGGTCGATGATACCGCCGGTGTCAT
CGCCCGCCTGCAGGAGCGTTTTCCCAACCTGACGGCGCCGGCCGCCGACTCGATCTGTTATGCGACGACCAACCGTCAGG
AAGTGGTGAAGCAGGCGGCCCCCGGCTGCGATCTCTTCATCATCGTCGGCGCGCCGAATTCCTCCAATTCCAAGCGCCTC
GTCGAAGTGGCGCTGCGGGCAGGGGCGAAGAAATCGATCCTGGTGCAGCGCGCCGCCGAGCTTGATTGGGATGAGATCGG
CGCGATTTCGACGCTCGGCCTTTCCGCCGGCGCCTCCGCCCCCGAGGTCATCGTCAACGAGATCATCGAGGCCTTCCGCG
CCCGCTTCGACGCCCGCGTCGAGCTGGCCGAAACGGTGCAGGAAACCGAGAATTTCCTGGTCAACCGCGAATTGCGCAGC
ATCGAGCTGACGGCGGCCGACATGGCCTTCGTCAACGGCTGA

Upstream 100 bases:

>100_bases
CTGGTTTCGCAAGCCGGGCAAGCAGCCGGCGCAATAGTATCGCTTCAATTTCCACCTATATTGGGCTAGAGGTCCCTAAA
TCTCTCAATCCGGAACAGAC

Downstream 100 bases:

>100_bases
TCCCCAGACGGAAATCCTATCGACGCCGGATGGTCGATCCGGTGGCCCAATAACCGCAATGCATGAAGGCGAGACCTCAC
TTGGCAGTCTATACCGATAT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNIAAKPPLTIRLCGPRGFCAGVDRAIQIVVLALKSYGAPVYVRHEIVHNRYVVEGLEAKGAVFVEELDEIPAEHRAQPV
VFSAHGVPKSVPEDAVSRNLFYLDATCPLVSKVHKQAMRHNRLGRHVVLIGHAGHPEVIGTMGQLPEGSVSLIETIEDAD
AYIPVDADNLGYVTQTTLSVDDTAGVIARLQERFPNLTAPAADSICYATTNRQEVVKQAAPGCDLFIIVGAPNSSNSKRL
VEVALRAGAKKSILVQRAAELDWDEIGAISTLGLSAGASAPEVIVNEIIEAFRARFDARVELAETVQETENFLVNRELRS
IELTAADMAFVNG

Sequences:

>Translated_333_residues
MNIAAKPPLTIRLCGPRGFCAGVDRAIQIVVLALKSYGAPVYVRHEIVHNRYVVEGLEAKGAVFVEELDEIPAEHRAQPV
VFSAHGVPKSVPEDAVSRNLFYLDATCPLVSKVHKQAMRHNRLGRHVVLIGHAGHPEVIGTMGQLPEGSVSLIETIEDAD
AYIPVDADNLGYVTQTTLSVDDTAGVIARLQERFPNLTAPAADSICYATTNRQEVVKQAAPGCDLFIIVGAPNSSNSKRL
VEVALRAGAKKSILVQRAAELDWDEIGAISTLGLSAGASAPEVIVNEIIEAFRARFDARVELAETVQETENFLVNRELRS
IELTAADMAFVNG
>Mature_333_residues
MNIAAKPPLTIRLCGPRGFCAGVDRAIQIVVLALKSYGAPVYVRHEIVHNRYVVEGLEAKGAVFVEELDEIPAEHRAQPV
VFSAHGVPKSVPEDAVSRNLFYLDATCPLVSKVHKQAMRHNRLGRHVVLIGHAGHPEVIGTMGQLPEGSVSLIETIEDAD
AYIPVDADNLGYVTQTTLSVDDTAGVIARLQERFPNLTAPAADSICYATTNRQEVVKQAAPGCDLFIIVGAPNSSNSKRL
VEVALRAGAKKSILVQRAAELDWDEIGAISTLGLSAGASAPEVIVNEIIEAFRARFDARVELAETVQETENFLVNRELRS
IELTAADMAFVNG

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=313, Percent_Identity=47.2843450479233, Blast_Score=287, Evalue=6e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35903; Mature: 35903

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAAKPPLTIRLCGPRGFCAGVDRAIQIVVLALKSYGAPVYVRHEIVHNRYVVEGLEAK
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHEEECCCCC
GAVFVEELDEIPAEHRAQPVVFSAHGVPKSVPEDAVSRNLFYLDATCPLVSKVHKQAMRH
CCHHHHHHHHCCHHHCCCCEEEECCCCCCCCCHHHHHCCEEEEECCCHHHHHHHHHHHHH
NRLGRHVVLIGHAGHPEVIGTMGQLPEGSVSLIETIEDADAYIPVDADNLGYVTQTTLSV
HCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHCCCCEEECCCCCCCCEEEEEEEC
DDTAGVIARLQERFPNLTAPAADSICYATTNRQEVVKQAAPGCDLFIIVGAPNSSNSKRL
CHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCHHH
VEVALRAGAKKSILVQRAAELDWDEIGAISTLGLSAGASAPEVIVNEIIEAFRARFDARV
HHHHHHCCCCHHHHHHHHHHCCHHHHCCHHEECCCCCCCCHHHHHHHHHHHHHHHHCCHH
ELAETVQETENFLVNRELRSIELTAADMAFVNG
HHHHHHHHHHHHHHCCCHHEEEEEEHHEEEECC
>Mature Secondary Structure
MNIAAKPPLTIRLCGPRGFCAGVDRAIQIVVLALKSYGAPVYVRHEIVHNRYVVEGLEAK
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCHHEEECCCCC
GAVFVEELDEIPAEHRAQPVVFSAHGVPKSVPEDAVSRNLFYLDATCPLVSKVHKQAMRH
CCHHHHHHHHCCHHHCCCCEEEECCCCCCCCCHHHHHCCEEEEECCCHHHHHHHHHHHHH
NRLGRHVVLIGHAGHPEVIGTMGQLPEGSVSLIETIEDADAYIPVDADNLGYVTQTTLSV
HCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHCCCCEEECCCCCCCCEEEEEEEC
DDTAGVIARLQERFPNLTAPAADSICYATTNRQEVVKQAAPGCDLFIIVGAPNSSNSKRL
CHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCHHH
VEVALRAGAKKSILVQRAAELDWDEIGAISTLGLSAGASAPEVIVNEIIEAFRARFDARV
HHHHHHCCCCHHHHHHHHHHCCHHHHCCHHEECCCCCCCCHHHHHHHHHHHHHHHHCCHH
ELAETVQETENFLVNRELRSIELTAADMAFVNG
HHHHHHHHHHHHHHCCCHHEEEEEEHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA